Bodenorganismen mögen es ruhig

Im Ackerbau sitzt man in Sachen Bodenbearbeitung zwischen zwei Stühlen. Zum einen bringt eine intensive Bodenbearbeitung zumindest kurzfristig oft agronomische Vorteile, zum anderen birgt sie ein hohes Erosionsrisiko und beeinträchtigt das Bodenleben stark. Es gilt, einen Mittelweg zu finden, der eine nachhaltige und bodenschonende Bewirtschaftung ermöglicht, ohne die momentane landwirtschaftliche Produktivität zu reduzieren

High‐Resolution Capillary Printing of Eutectic Gallium Alloys for Printed Electronics

A versatile liquid metal (LM) printing process enabling the fabrication of various fully printed devices such as intra- and interconnect wires, resistors, diodes, transistors, and basic circuit elements such as inverters which are process compatible with other digital printing and thin film structuring methods for integration is presented. For this, a glass capillary-based direct-write method for printing LMs such as eutectic gallium alloys, exploring the potential for fully printed LM-enabled devices is demonstrated. Examples for successful device fabrication include resistors, p–n diodes, and field effect transistors. The device functionality and easiness of one integrated fabrication flow shows that the potential of LM printing is far exceeding the use of interconnecting conventional electronic devices in printed electronics

BP180-specific IgG is associated with skin adverse events, therapy response and overall survival in non-small cell lung cancer patients treated with checkpoint inhibitors

BACKGROUND: Anti-PD1/PD-L1 therapy frequently entails immune-related adverse events (irAEs) and biomarkers to predict irAEs are lacking. While checkpoint inhibitors have been found to re-invigorate T-cells, the relevance of autoantibodies remains elusive. OBJECTIVE: Our aim was to explore whether IgG autoantibodies directed against co-expressed antigens by tumor tissue and healthy skin correlate with skin irAEs and therapy outcome. METHODS: We measured skin-specific IgG via ELISA in non-small cell lung cancer (NSCLC) patients, who received anti-PD1/PD-L1 treatment between July 2015 and September 2017 at the Kantonsspital St. Gallen. Sera were sampled at baseline and during therapy after 8 weeks. RESULTS: Analysis of publicly available tumor expression data revealed that NSCLC and skin co-express BP180, BP230 and type VII collagen. Of 40 recruited patients, 16 (40%) developed a skin irAE. Only elevated anti-BP180 IgG at baseline significantly correlated with the development of skin irAEs (P=.04), therapy response (P=.01) and overall survival (P=.04). LIMITATIONS: The patients were recruited in a single tertiary care center. CONCLUSIONS: Our data suggest that the level of anti-BP180 IgG of NSCLC patients at baseline is associated with better therapy response, overall survival and a higher probability to develop skin irAEs during anti-PD1/PD-L1 treatment

Základy půdní úrodnosti

Zvyšování půdní úrodnosti bylo pro průkopníky ekologického zemědělství základem veškerého jejich úsilí. Přesto zachování úrodné půdy mnohdy nebyla věnována dostatečná pozornost. Ekologické zemědělství je však na přirozené půdní úrodnosti závislé. Oslabená a poškozená půda nám nemůže poskytnout to, co od ní očekáváme. Udržet úrodnost půdy vyžaduje velkou péči. Předkládaná brožura ukazuje půdní úrodnost z různých úhlů pohledu. Naším záměrem však nebylo vytvořit obecně platný „návod k použití“. Informace mají být mnohem spíše podnětem k tomu, aby se o vztahu člověka k půdě smýšlelo jinak a aby se tento vztah utvářel ve prospěch budoucnosti

Grundlagen zur Bodenfruchtbarkeit - Die Beziehung zum Boden gestalten

Die Broschüre beleuchtet die Bodenfruchtbarkeit aus verschiedenen wissenschaftlichen und bäuerlichen Blickwinkeln. Die Informationen wollen praktische Beobachtungen der Landwirte ergänzen und dazu anregen, die Beziehung zum Boden zu überdenken und eine wirklich nachhaltige Bodenkultur zu praktizieren

Immune signatures predict development of autoimmune toxicity in patients with cancer treated with immune checkpoint inhibitors.

BACKGROUND Immune checkpoint inhibitors (ICIs) are among the most promising treatment options for melanoma and non-small cell lung cancer (NSCLC). While ICIs can induce effective anti-tumor responses, they may also drive serious immune-related adverse events (irAEs). Identifying biomarkers to predict which patients will suffer from irAEs would enable more accurate clinical risk-benefit analysis for ICI treatment and may also shed light on common or distinct mechanisms underpinning treatment success and irAEs. METHODS In this prospective multi-center study, we combined a multi-omics approach including unbiased single-cell profiling of over 300 peripheral blood mononuclear cell (PBMC) samples and high-throughput proteomics analysis of over 500 serum samples to characterize the systemic immune compartment of patients with melanoma or NSCLC before and during treatment with ICIs. FINDINGS When we combined the parameters obtained from the multi-omics profiling of patient blood and serum, we identified potential predictive biomarkers for ICI-induced irAEs. Specifically, an early increase in CXCL9/CXCL10/CXCL11 and interferon-γ (IFN-γ) 1 to 2 weeks after the start of therapy are likely indicators of heightened risk of developing irAEs. In addition, an early expansion of Ki-67+ regulatory T cells (Tregs) and Ki-67+ CD8+ T cells is also likely to be associated with increased risk of irAEs. CONCLUSIONS We suggest that the combination of these cellular and proteomic biomarkers may help to predict which patients are likely to benefit most from ICI therapy and those requiring intensive monitoring for irAEs. FUNDING This work was primarily funded by the European Research Council, the Swiss National Science Foundation, the Swiss Cancer League, and the Forschungsförderung of the Kantonsspital St. Gallen

Value of ultrasonography as a marker of early response to abatacept in patients with rheumatoid arthritis and an inadequate response to methotrexate: results from the APPRAISE study

Objectives: To study the responsiveness of a combined power Doppler and greyscale ultrasound (PDUS) score for assessing synovitis in biologic-naïve patients with rheumatoid arthritis (RA) starting abatacept plus methotrexate (MTX). Methods: In this open-label, multicentre, single-arm study, patients with RA (MTX inadequate responders) received intravenous abatacept (∼10 mg/kg) plus MTX for 24 weeks. A composite PDUS synovitis score, developed by the Outcome Measures in Rheumatology–European League Against Rheumatism (OMERACT–EULAR)-Ultrasound Task Force, was used to evaluate individual joints. The maximal score of each joint was added into a Global OMERACT–EULAR Synovitis Score (GLOESS) for bilateral metacarpophalangeal joints (MCPs) 2–5 (primary objective). The value of GLOESS containing other joint sets was explored, along with clinical efficacy. Results: Eighty-nine patients completed the 24-week treatment period. The earliest PDUS sign of improvement in synovitis was at week 1 (mean change in GLOESS (MCPs 2–5): −0.7 (95% CIs −1.2 to −0.1)), with continuous improvement to week 24. Early improvement was observed in the component scores (power Doppler signal at week 1, synovial hyperplasia at week 2, joint effusion at week 4). Comparable changes were observed for 22 paired joints and minimal joint subsets. Mean Disease Activity Score 28 (C reactive protein) was significantly reduced from weeks 1 to 24, reaching clinical meaningful improvement (change ≥1.2) at week 8. Conclusions: In this first international prospective study, the composite PDUS score is responsive to abatacept. GLOESS demonstrated the rapid onset of action of abatacept, regardless of the number of joints examined. Ultrasound is an objective tool to monitor patients with RA under treatment. Trial registration number: NCT00767325

Crop yield and soil fertility response to reduced tillage under organic management

Conservation tillage (no-till and reduced tillage) brings many benefits with respect to soil fertility and energy use, but it also has drawbacks regarding the need for synthetic fertilizers and herbicides. Our objective was to adapt reduced tillage to organic farming by quantifying effects of tillage (plough versus chisel), fertilization (slurry versus manure compost) and biodynamic preparations (with versus without) on soil fertility indicators and crop yield. The experiment was initiated in 2002 on a Stagnic Eutric Cambisol (45% clay content) near Frick (Switzerland) where the average annual precipitation is 1000 mm. This report focuses on the conversion period and examines changes as tillage intensity was reduced. Soil samples were taken from the 0-10 cm and 10-20 cm depths and analysed for soil organic carbon (Corg), microbial biomass (Cmic), dehydrogenase activity (DHA) and earthworm density and biomass. Among the components tested, only tillage had any influence on these soil fertility indicators. Corg in the 0-10 cm soil layer increased by 7.4% (1.5 g Corg kg-1 soil, p < 0.001) with reduced tillage between 2002 and 2005, but remained constant with conventional tillage. Similarly, Cmic was 28% higher and DHA 27% (p < 0.001) higher with reduced than with conventional tillage in the soil layer 0-10 cm. In the 10-20 cm layer, there were no significant differences for these soil parameters between the tillage treatments. Tillage had no significant effect on total earthworm density and biomass. The abundance of endogeic, horizontally burrowing adult earthworms was 70% higher under reduced than conventional tillage but their biomass was 53% lower with reduced tillage. Wheat (Triticum aestivum L.) and spelt (Triticum spelta L.) yield decreased by 14% (p < 0.001) and 8% (p < 0.05), respectively, with reduced tillage, but sunflower (Helianthus annuus L.) yield was slightly higher with reduced tillage. Slurry fertilization enhanced wheat yield by 5% (p < 0.001) compared to compost fertilization. Overall, Corg, Cmic, and DHA improved and yields showed only a small reduction with reduced tillage under organic management, but long-term effects such as weed competition remain unknown

Greedy optimization of resistance-based graph robustness with global and local edge insertions

The total effective resistance, also called the Kirchhoff index, provides a robustness measure for a graph G. We consider two optimization problems of adding k new edges to G such that the resulting graph has minimal total effective resistance (i.e., is most robust)—one where the new edges can be anywhere in the graph and one where the new edges need to be incident to a specified focus node. The total effective resistance and effective resistances between nodes can be computed using the pseudoinverse of the graph Laplacian. The pseudoinverse may be computed explicitly via pseudoinversion, yet this takes cubic time in practice and quadratic space. We instead exploit combinatorial and algebraic connections to speed up gain computations in an established generic greedy heuristic. Moreover, we leverage existing randomized techniques to boost the performance of our approaches by introducing a sub-sampling step. Our different graph- and matrix-based approaches are indeed significantly faster than the state-of-the-art greedy algorithm, while their quality remains reasonably high and is often quite close. Our experiments show that we can now process larger graphs for which the application of the state-of-the-art greedy approach was impractical before.Quantum & Computer Engineerin

Influence of reduced tillage on earthworm and microbial communities under organic arable farming

Although reduced tillage is an agricultural practice reported to decrease soil erosion and external inputs while enhancing soil fertility it has still rarely been adopted by European organic farmers. The objective of this study was to assess the long-term interactive effects of tillage [conventional (CT) versus reduced (RT)] and fertilization [slurry (S) versus composted manure/slurry (MCS)] on earthworms and microbial communities in a clay soil under spelt in an organic 6-year crop rotation. Earthworm populations (species, density and biomass, cocoons) were investigated by handsorting the soil nine years after initial implementation of the treatments. Soil microbial carbon (Cmic) and nitrogen (Nmic) were measured by chloroform-fumigation extraction and a simplified phospholipid fatty acid (PLFA) analysis was used to separate for populations of bacteria, fungi and protozoa. Total earthworm density was significantly increased in RT plots mainly being related to increased numbers of juveniles. Moreover, we found five times more cocoons with RT. Species richness was not affected by the treatments, but tillage treatments have differentially altered populations on species-level. In addition, cluster analysis on community level revealed two distinct groups of plots in relation to tillage treatments. In RT plots Cmic increased in the 0-10 cm and 10-20 cm soil layers, while PLFA concentrations indicative of Gram-negative bacteria, fungi and protozoa only increased in the topsoil. Lower bacteria-to-fungi ratios in the upper soil layer of RT plots indicated a shift to fungalbased decomposition of organic matter whereas a higher Cmic-to-Corg ratio pointed towards enhanced substrate availability. Slurry application decreased microbial biomass and enhanced density of juvenile anecic earthworms but overall fertilization effect was weak and no interactions with tillage were found. In conclusion, tillage is a major driver in altering communities of earthworms and microorganisms in arable soils. The use of reduced tillage provides an approach for eco-intensification by enhancing inherent soil biota functions under organic arable farming