“Nanopartículas de Hierro con Tamaño Controlado Depositadas sobre Clinoptilolita por la Técnica de Electroless Plating Method- Coprecipitación: Síntesis y Caracterización”

En el presente trabajo se ha abordado el estudio de la concentración de HCl sobre la activación con SnCl2 de una Zeolita sintetica tipo A de Sodio, que denominaremos Clinoptilolita (CLT), para la depositación en su superficie de óxidos de hierro de tamaño nanomaetrico mediante una técnica conjunta de Electroless Plating Method-Coprecipitación. La razón de utilizar una zeolita como sustrato surge de querer aprovechar sus dimensiones para obtener depósitos de tamaño nanométrico, esto con el fin de que el sustrato sea evaluado a futuro como un material compuesto que presente propiedades magnéticas, de catálisis heterogénea, conducción térmica o de reforzamiento mecánico, esta ultima si se le llegase a utilizar como carga en polímeros

The social neuroscience and the theory of integrative levels

Frontiers in Integrative Neuroscience 9 (2015): A054 This Document is Protected by copyright and was first published by Frontiers. All rights reserved. it is reproduced with permissionThe theory of integrative levels provides a general description of the evolution of matter through successive orders of complexity and integration. Along its development, material forms pass through different levels of organization, such as physical, chemical, biological or sociological. The appearance of novel structures and dynamics during this process of development of matter in complex systems has been called emergence. Social neuroscience (SN), an interdisciplinary field that aims to investigate the biological mechanisms that underlie social structures, processes, and behavior and the influences between social and biological levels of organization, has affirmed the necessity for including social context as an essential element to understand the human behavior. To do this, SN proposes a multilevel integrative approach by means of three principles: multiple determinism, nonadditive determinism and reciprocal determinism. These theoretical principles seem to share the basic tenets of the theory of integrative levels but, in this paper, we aim to reveal the differences among both doctrines. First, SN asserts that combination of neural and social variables can produce emergent phenomena that would not be predictable from a neuroscientific or social psychological analysis alone; SN also suggests that to achieve a complete understanding of social structures we should use an integrative analysis that encompasses levels of organization ranging from the genetic level to the social one; finally, SN establishes that there can be mutual influences between biological and social factors in determining behavior, accepting, therefore, a double influence, upward from biology to social level, and downward, from social level to biology. In contrast, following the theory of integrative levels, emergent phenomena are not produced by the combination of variables from two levels, but by the increment of complexity at one level. In addition, the social behavior and structures might be contemplated not as the result of mixing or summing social and biological influences, but as emergent phenomena that should be described with its own laws. Finally, following the integrative levels view, influences upward, from biology to social level, and downward, from social level to biology, might not be equivalent, since the bottom-up processes are emergent and the downward causation (DC) is notThis work was funded by the Spanish Ministry of Science (BFU2011-29089) to JMD-

Propuesta de reforma al Artículo 31 fracción IV de la Constitución Política de los Estados Unidos Mexicanos por ausencia de algunos principios de justicia tributaria

Tesis (Doctor en Derecho) U.A.N.L.UANLhttp://www.uanl.mx

Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews)

As an alternative to conventional lighting systems, light emitting diode (LED) has been demonstrated to be an artificial flexible lighting source for commercial micropropagation. The objective of this study was to determine the effects of different LED light quality on in vitro shoot proliferation and growth of Vanilla planifolia. To evaluate shoot proliferation, axillary bud cuttings (3 to 5 mm in diameter) of V. planifolia were used as explants and cultivated on Murashige and Skoog basal medium supplemented with 9.55 μM of 6-benzylaminopurine. To evaluate in vitro growth, unrooted shoots (2 cm in length) were used as explants cultivated on Murashige and Skoog basal medium without plant growth regulators. All explants were exposed to a 16 h photoperiod for 60 days under five different lights: fluorescent lamp, white LED, blue LED, red LED and blue plus red LED mixtures (Blue + Red = 1:1). The results indicated a clear increase in the number of shoots per explant under Fluorescent Lamp, White LED and Blue+Red LED light; these treatments produced more than 10 shoots. Shoot length was more than 3 cm in cultures under Blue, Red and Blue+Red (1:1) LEDs, and less than 3 cm in Fluorescent Lamp and White LEDs. Our results also showed that fresh weight, dry weight and dry matter were greatest in shoots under Blue+Red LED light. For shoot growth, plant height, number of leaves, number and length of roots, fresh weight, dry weight, dry matter and chlorophyll content were greater under Fluorescent Lamp and White LED. In conclusion, White or Blue+Red LED light may be used as an alternative light source for shoot proliferation, while White LED may be used for growth in vitro. These results demonstrate the effectiveness of light qualities using LEDs for micropropagation of V. planifolia.Key words: Light quality, micropropagation, orchid, chlorophyll

Nuevos criterios estético-funcionales de las pantallas antirruido construidas de hormigón

The noise produced by road traffic can be abated either at the source (the traffic) or at the receiver (housing) or in external space. From the operational point of view, the most efficient and inmediat way of achieving this acoustic abatement is to situate some barriers near the road that interrumpt the path of the sonic waves, lessening the impact on the receiver until it reaches acceptable levels. An antinoise screen, because of its physical characteristics must fullfil two funtions: acoustic and aesthetic. The first one justifies its own viability and the second requieres an adequate insertion in urban spaces. In this work some basic action criteria that serves to guide in the obtaining an efficient aesthetic-funtional position of the anti-noise screens by the sides of roads or railways are developped.El ruido producido por el tráfico vial puede atenuarse actuando en la fuente (el tráfico), en el receptor (las viviendas) o en los espacios exteriores. Desde el aspecto operativo, la vía más eficaz e inmediata de conseguir esta atenuación acústica es situar unas pantallas próximas a las vías que, interrumpiendo la trayectoria de la onda sónica, amortigüe el impacto sobre el receptor hasta niveles aceptables. Una pantalla antirruido, por sus características físicas, debe cumplir dos funciones: acústica y estética. La primera justifica su propia viabilidad y la segunda exige una adecuada inserción en los espacios urbanos. En este trabajo se exponen unos criterios básicos de actuación que sirvan de orientación para conseguir una eficaz ubicación estético funcional de las pantallas antirruido en las márgenes de las carreteras o de los ferrocarriles

New insights into the regulation of NADPH oxidase dependent reactive oxygen species signaling during the plant immune response

Las NADPH oxidasas de plantas, denominadas “respiratory burst oxidase homologues” (RBOHs), producen especies reactivas del oxígeno (ROS) que median un amplio rango de funciones. En la célula vegetal, el ajuste preciso de la producción de ROS aporta la especificidad de señal para generar una respuesta apropiada ante las amenazas ambientales. RbohD y RbohF, dos de los diez genes Rboh de Arabidopsis, son pleiotrópicos y median diversos procesos fisiológicos en respuesta a patógenos. El control espacio-temporal de la expresión de los genes RbohD y RbohF podría ser un aspecto crítico para determinar la multiplicidad de funciones de estas oxidasas. Por ello, generamos líneas transgénicas de Arabidopsis con fusiones de los promoters de RbohD y RbohF a los genes delatores de la B-glucuronidasa y la luciferasa. Estas líneas fueron empleadas para revelar el patrón de expresión diferencial de RbohD y RbohF durante la respuesta inmune de Arabidopsis a la bacteria patógena Pseudomonas syringae pv. tomato DC3000, el hongo necrótrofo Plectosphaerella cucumerina y en respuesta a señales relacionadas con la respuesta inmune. Nuestros experimentos revelan un patrón de expresión diferencial de los promotores de RbohD y RbohF durante el desarrollo de la planta y en la respuesta inmune de Arabidopsis. Además hemos puesto de manifiesto que existe una correlación entre el nivel de actividad de los promotores de RbohD y RbohF con la acumulación de ROS y el nivel de muerte celular en respuesta a patógenos. La expression de RbohD y RbohF también es modulada de manera diferencial en respuesta a patrones moleculares asociados a patógenos (PAMPs) y por ácido abscísico (ABA). Cabe destacar que, mediante una estrategia de intercambio de promotores, hemos revelado que la región promotora de RbohD, es necesaria para dirigir la producción de ROS en respuesta a P. cucumerina. Adicionalmente, la activación del promotor de RbohD en respuesta al aislado de P. cucumerina no adaptado a Arabidopsis 2127, nos llevó a realizar ensayos de susceptibilidad con el doble mutante rbohD rbohF que han revelado un papel desconocido de estas oxidasas en resistencia no-huesped. La interacción entre la señalización dependiente de las RBOHs y otros componentes de la respuesta inmune de plantas podría explicar también las distintas funciones que median estas oxidasas en relación con la respuesta inmune. Entre la gran cantidad de señales coordinadas con la actividad de las RBOHs, existen evidencias genéticas y farmacológicas que indican que las proteínas G heterotriméricas están implicadas en algunas de las rutas de señalización mediadas por ROS derivadas de los RBOHs en respuesta a señales ambientales. Por ello hemos estudiado la relación entre estas RBOH-NADPH oxidasas y AGB1, la subunidad β de las proteínas G heterotriméricas en la respuesta inmune de Arabidopsis. Análisis de epistasis indican que las proteínas G heterotriméricas están implicadas en distintas rutas de señalización en defensa mediadas por las RBOHs. Nuestros resultados ilustran la relación compleja entre la señalización mediada por las RBOHs y las proteínas G heterotriméricas, que varía en función de la interacción planta-patógeno analizada. Además, hemos explorado la posible asociación entre AGB1 con RBOHD y RBOHF en eventos tempranos de la respuesta immune. Cabe señalar que experimentos de coímmunoprecipitación apuntan a una posible asociación entre AGB1 y la kinasa citoplasmática reguladora de RBOHD, BIK1. Esto indica un posible mecanismo de control de la función de esta NADPH oxidase por AGB1. En conjunto, estos datos aportan nuevas perspectivas sobre cómo, a través del control transcripcional o mediante la interacción con las proteínas G heterotriméricas, las NADPH oxidases de plantas median la producción de ROS y la señalización por ROS en la respuesta inmune. Nuestro trabajo ejemplifica cómo la regulación diferencial de dos miembros de una familia multigénica, les permite realizar distintas funciones fisiológicas especializadas usando un mismo mecanismo enzimático. ABSTRACT The plant NADPH oxidases, termed respiratory burst oxidase homologues (RBOHs), produce reactive oxygen species (ROS) which mediate a wide range of functions. Fine tuning this ROS production provides the signaling specificity to the plant cell to produce the appropriate response to environmental threats. RbohD and RbohF, two of the ten Rboh genes present in Arabidopsis, are pleiotropic and mediate diverse physiological processes in response to pathogens. One aspect that may prove critical to determine the multiplicity of functions of RbohD and RbohF is the spatio-temporal control of their gene expression. Thus, we generated Arabidopsis transgenic lines with RbohD- and RbohF-promoter fusions to the β-glucuronidase and the luciferase reporter genes. These transgenics were employed to reveal RbohD and RbohF promoter activity during Arabidopsis immune response to the pathogenic bacterium Pseudomonas syringae pv tomato DC3000, the necrotrophic fungus Plectosphaerella cucumerina and in response to immunity-related cues. Our experiments revealed a differential expression pattern of RbohD and RbohF throughout plant development and during Arabidopsis immune response. Moreover, we observed a correlation between the level of RbohD and RbohF promoter activity, the accumulation of ROS and the amount of cell death in response to pathogens. RbohD and RbohF gene expression was also differentially modulated by pathogen associated molecular patterns and abscisic acid. Interestingly, a promoter-swap strategy revealed the requirement for the promoter region of RbohD to drive the production of ROS in response to P. cucumerina. Additionally, since the RbohD promoter was activated during Arabidopsis interaction with a non-adapted P. cucumerina isolate 2127, we performed susceptibility tests to this fungal isolate that uncovered a new role of these oxidases on non-host resistance. The interplay between RBOH-dependent signaling with other components of the plant immune response might also explain the different immunity-related functions mediated by these oxidases. Among the plethora of signals coordinated with RBOH activity, pharmacological and genetic evidence indicates that heterotrimeric G proteins are involved in some of the signaling pathways mediated by RBOH–derived ROS in response to environmental cues. Therefore, we analysed the interplay between these RBOH-NADPH oxidases and AGB1, the Arabidopsis β-subunit of heterotrimeric G proteins during Arabidopsis immune response. We carried out epistasis studies that allowed us to test the implication of AGB1 in different RBOH-mediated defense signaling pathways. Our results illustrate the complex relationship between RBOH and heterotrimeric G proteins signaling, that varies depending on the type of plant-pathogen interaction. Furthermore, we tested the potential association between AGB1 with RBOHD and RBOHF during early immunity. Interestingly, our co-immunoprecipitation experiments point towards an association of AGB1 and the RBOHD regulatory kinase BIK1, thus providing a putative mechanism in the control of the NADPH oxidase function by AGB1. Taken all together, these studies provide further insights into the role that transcriptional control or the interaction with heterotrimeric G-proteins have on RBOH-NADPH oxidase-dependent ROS production and signaling in immunity. Our work exemplifies how, through a differential regulation, two members of a multigenic family achieve specialized physiological functions using a common enzymatic mechanism

Infección de células oligodendrocíticas con el virus Herpes simplex tipo 1: efecto sobre el proteolípido MAL2

Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular . Fecha de lectura: 22-10-200

A homopolar disc dynamo experiment with liquid metal contacts

We present experimental results of a homopolar disc dynamo constructed at CICATA-Quer\'etaro in Mexico. The device consists of a flat, multi-arm spiral coil which is placed above a fast-spinning metal disc and connected to the latter by sliding liquid-metal electrical contacts. Theoretically, self-excitation of the magnetic field is expected at the critical magnetic Reynolds number Rm~45, which corresponds to a critical rotation rate of about 10 Hz. We measured the magnetic field above the disc and the voltage drop on the coil for the rotation rate up to 14 Hz, at which the liquid metal started to leak from the outer sliding contact. Instead of the steady magnetic field predicted by the theory we detected a strongly fluctuating magnetic field with a strength comparable to that of Earth's magnetic field which was accompanied by similar voltage fluctuations in the coil. These fluctuations seem to be caused by the intermittent electrical contact through the liquid metal. The experimental results suggest that the dynamo with the actual electrical resistance of liquid metal contacts could be excited at the rotation rate of around 21 Hz provided that the leakage of liquid metal is prevented.Comment: 6 pages, 5 figures (to appear in Magnetohydrodynamics