A Administração estratégica no gerenciamento dos recursos humanos e materiais do 8º Batalhao de Polícia Militar de Joinville

Orientador: Marcio S. B. S. de OliveiraCo-orientador: Alvir Antonio SchneiderMonografia (Especialização) - Universidade Federal do Paraná, Setor de Ciências Sociais Aplicadas, Curso de Especialização em Planejamento e Controle em Segurança PúblicaResumo: Muitas das necessidades básicas da sociedade não podem ser supridas pelas organizações do setor privado. Por isso existem as organizações do setor público. Mas, assim como nas organizações privadas, as organizações públicas não podem se privar de cuidados quanto ao posicionamento e relacionamento com o ambiente interno e externo, de forma a assegurar resultados continuados e antecipar eventuais surpresas, sob pena de serem mal interpretadas pela sociedade. Nessa contexto, esta a Polícia Militar, com a função de preservar a ordem pública. Considerando que, para que a organizações possa otimizar seus resultados deve realizar urn ajustamento interativo entre as mudanças nos fatores ambientais os fatores internos, pode-se observar que, em se tratando da força policial, tal ajustamento não esta sendo realizado. Os recursos humanos e materiais, necessários para o born desempenho das atividades da Polícia Militar, não tem acompanhado os números apresentados de crescimento das cidades, da violência e da criminalidade. Como a administração estratégica envolve a interação entre as mudanças nos fatores ambientais e os fatores internos da organização, pretende-se com o trabalho, por meio de urn estudo desenvolvido no 8° Batalhão de Polícia Militar de Joinville, demonstrar que a administração estratégica pode contribuir no gerenciamento eficiente das Organizações da Polícia Militar, racionalizando e otimizando o uso dos recursos escasso

A new peptide from Jatropha curcas seeds: Unusual sequence and insights into its synthetic analogue that enhances proteolytic activity of papain

AbstractA new peptide (1341g/mol) from Jatropha curcas seeds was isolated. The linear sequence (APTLSGGSVPRDAD) was deduced by de novo peptide sequencing, and further used as scaffold for synthesis of linear (1342g/mol) and cyclic (1324g/mol) synthetic analogues. The full peptide sequence was identified as inserted in a putative conserved domain of late-embryogenesis proteins which produced a significant alignment hit (100% of identity and E-value of 1e−05) with a hypothetical protein JCGZ_12502 of J. curcas. Whereas in the linear peptide predominated the double charged ion state (m/z 671.68), in the cyclic form was observed the mono charged ion state (m/z of 1325.19) and an unusual MS/MS fragmentation pattern. The differences between the forms were discrete in terms of ionic mobility, retention time (reverse phase) and net charge as function of pH. Circular dichroism spectra presented an intense negative peak at 198nm which is assigned for its disordered contents. A negative peak at 222nm in the spectrum of the circular form suggested its structure was not as disordered as the linear form. The peptides were neither haemolytic nor cytotoxic and did not inhibit phytopathogenic fungi. Surprisingly, the circular but not the linear peptide increased the proteolytic activity of papain

Avaliação da toxicidade oral subcrônica da bixina para ratos

The aim of the investigation was to determine the possible health hazards of bixin (30%) from annatto (Bixa orellana L.) origin to rats. A concentration of 0.01±0.006%/day of bixin in corn oil was administered to 20 Wistar rats (10 per sex), through the oral route (gavage) over a period of 13 weeks. A group of untreated animal (10 per sex) acting as a control (corn oil) was used for comparision. Body weight, body weight gain, feed consumption and feed efficiency were determined. The health of the animals was checked. At the end of the study a biochemistry (glucose, creatinine, total cholesterol, triglyceride, asparagine transaminase and g-glutamyl transaminase) and hematological examination was carried out. All the animals were subjected to a gross-pathological assessment followed by liver, kidney, adrenals, spleen and tests weight. A histopathological analysis (liver and kidney) was performed. From the weight parameters clinical, clinical chemistry and haematology, necroscopy and histology viewpoints, it can be said that annatto, under the chosen test condition, was no toxic to the rat.A bixina em pó (30% de bixina), proveniente das sementes de urucum (Bixa orellana L.), foi administrada, por gavagem, a ratos Wistar, 10 animais de cada sexo, na concentração de 0,01±0,006% de bixina/dia, em óleo de milho, cinco dias por semana, durante 13 semanas, com o objetivo de verificar a toxicidade da substância-teste para essa espécie animal. A grupos controle (10 animais por sexo), foi administrado óleo de milho, para comparação. Durante o período de exposição, foram registrados o peso absoluto corpóreo, o ganho de peso, o consumo de ração e a eficiência alimentar, bem como realizadas as avaliações clínica e oftalmoscópica. Antes da eutanásia, os animais foram anestesiados (éter etílico) e submetidos a exames hematológicos de rotina e bioquímicos (glicose, creatinina, colesterol total, triglicérides, asparagina transaminase e g-glutamil transaminase). Durante o exame necroscópico, fígado, rins, baço, adrenais e testículos foram excisados e pesados. O estudo histológico foi realizado em amostras de fígado e rins dos animais expostos e respectivos controles. A análise estatística dos parâmetros de peso, hematológicos e bioquímicos mostrou algumas diferenças significativas entre os grupos teste e controle, as quais não parecem estar relacionadas à exposição. Não foram observadas alterações clínicas, comportamentais, necroscópicas e histológicas. Nas condições do estudo, a bixina não produziu efeitos tóxicos nos animais expostos

Alcohol Abuse Promotes Changes in Non-Synaptic Epileptiform Activity with Concomitant Expression Changes in Cotransporters and Glial Cells

Non-synaptic mechanisms are being considered the common factor of brain damage in status epilepticus and alcohol intoxication. the present work reports the influence of the chronic use of ethanol on epileptic processes sustained by non-synaptic mechanisms. Adult male Wistar rats administered with ethanol (1, 2 e 3 g/kg/d) during 28 days were compared with Control. Non-synaptic epileptiform activities (NEAs) were induced by means of the zero-calcium and high-potassium model using hippocampal slices. the observed involvement of the dentate gyrus (DG) on the neurodegeneration promoted by ethanol motivated the monitoring of the electrophysiological activity in this region. the DG regions were analyzed for the presence of NKCC1, KCC2, GFAP and CD11b immunoreactivity and cell density. the treated groups showed extracellular potential measured at the granular layer with increased DC shift and population spikes (PS), which was remarkable for the group E1. the latencies to the NEAs onset were more prominent also for the treated groups, being correlated with the neuronal loss. in line with these findings were the predispositions of the treated slices for neuronal edema after NEAs induction, suggesting that restrict inter-cell space counteracts the neuronal loss and subsists the hyper-synchronism. the significant increase of the expressions of NKCC1 and CD11b for the treated groups confirms the existence of conditions favorable to the observed edematous necrosis. the data suggest that the ethanol consumption promotes changes on the non-synaptic mechanisms modulating the NEAs. for the lower ethanol dosage the neurophysiological changes were more effective suggesting to be due to the less intense neurodegenertation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Joao del Rei, Dept Engn Biossistemas, Lab Neurociencia Expt & Computac, Sao Joao Del Rei, MG, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Neurol Expt, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Fisiol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Neurol Expt, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Fisiol, São Paulo, BrazilWeb of Scienc

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost