ACOLHIMENTO PRESTADO AOS USUÁRIOS DO CAPS AD NA PERCEPÇÃO DOS TRABALHADORES DE SAÚDE: UMA REVISÃO INTEGRATIVA

A dependência química e o abuso de substâncias psicoativas tornou-se um grande problema de saúde pública (SANTANA., et al, 2018). Segundo relatório divulgado pela OMS em conjunto com A United Nations Office on Drugs and Crime (UNODC) cerca de 205 milhões de pessoas consomem drogas ilegais em todo o mundo das quais 25 milhões são dependentes. (UNODC, 2014)

É Possível Integrar, em um mesmo Conceito, os Vínculos Afetivo e Instrumental? O olhar de gestores sobre o comprometimento com a organização

O objetivo do presente estudo é compreender qual a visão que os gestores possuem do que é um trabalhador comprometido com a organização, explorando a pertinência de se tratar como integrantes do mesmo construto as dimensões afetiva e instrumental, conforme proposto por Meyer e Allen (1991). Para tanto, definiu-se caracterizar os elementos esquemáticos que compõem o conceito de trabalhador comprometido e explorar o peso dos elementos afetivos e instrumentais que integram este esquema. Os elementos esquemáticos foram identificados por meio da atribuição de rótulo verbal a dois per s distintos de trabalhador comprometido; a resposta espontânea a questionamento acerca do que é comprometimento e suas principais características e escolha de características de comprometimento disponíveis na literatura sobre o tema. Duas empresas de médio/grande porte da região metropolitana de Salvador-BA participaram do estudo por meio de oito gestores (quatro de cada organização). Os dados foram tratados por meio da análise de conteúdo e os resultados apresentados com o uso de técnicas de mapeamento cognitivo. Concluiu-se que os gestores pesquisados relacionam o conceito de comprometimento à sua base afetiva e consideram o comprometimento instrumental como sendo a antítese do comprometimento. Consideram que esforço-extra e contribuição adicional são importantes para que um trabalhador possa ser considerado como comprometido

Remodeling of gingival contour in the rehabilitation with fixed partial dentures – case report / Remodelação do contorno gengival em reabilitação com prótese parcial fixa – relato de caso

One of the most challenging aspects in rehabilitations is the correct manipulation of gingival tissues, aiming to achieve a natural contour, with interdental papillae and emergence profile, compatible with a natural tooth. The rehabilitation is impaired when there is loss of the interproximal papilla. To solve this problem, it is possible to use the gingival conditioning technique, which comprises relining the provisional crown with acrylic resin, applying gradual pressure, leading to formation of a gingival papilla. This paper presents a case of esthetic and functional recovery in a fixed partial denture by remodeling of the gingival contour by the gradual pressure technique, by gradual relining of the provisional crown. It is concluded that this gingival conditioning technique is simple, easy to accomplish and very effective for esthetic-functional reestablishment in rehabilitations with fixed partial dentures, provided the patient maintains correct hygiene and plaque control. 

Effects of Maté Tea Intake on ex Vivo LDL Peroxidation Induced by Three Different Pathways

Yerba maté (Ilex paraguariensis) is a native South America plant widely consumed as different beverages. Yerba maté leaves contains high concentrations of polyphenols that are responsible for its high in vitro and in vivo antioxidant activity. The in vivo antioxidant properties vis a vis LDL particles has not yet been studied for maté tea, the roasted yerba maté product. The aim of this study was to evaluate the antioxidant activity of maté tea ingestion ex vivo on human LDL. Fasting peripheral venous blood samples of healthy women were taken in three different times: before drinking the tea, one hour later and after one week (7 days) of daily consumption of maté tea. The isolated LDL was oxidized by three different pathways [copper (CuSO4), lipoxygenase and peroxynitrite (SIN-1)]. Conjugated dienes and structural modifications on LDL were evaluated. Ingestion of maté tea increased LDL resistance towards ex vivo copper oxidation, but did not alter the peroxidation pattern when SIN-1 or lipoxygenase were used as oxidant

Expression of osteoblastic phenotype in periodontal ligament fibroblasts cultured in three-dimensional collagen gel

Objective : To investigate the influence of a three-dimensional cell culture model on the expression of osteoblastic phenotype in human periodontal ligament fibroblast (hPDLF) cultures. Material and Methods : hPDLF were seeded on bi-dimensional (2D) and three-dimensional (3D) collagen type I (experimental groups) and and on a plastic coverslip (control) for up to 14 days. Cell viability and alkaline phosphatase (ALP) activity were performed. Also, cell morphology and immunolabeling for alkaline phosphatase (ALP) and osteopontin (OPN) were assessed by epifluorescence and confocal microscopy. The expression of osteogenic markers, including alkaline phosphatase, osteopontin, osteocalcin (OC), collagen I (COL I) and runt-related transcription factor 2 (RUNX2), were analyzed using real-time polymerase chain reaction (RT-PCR). Mineralized bone-like nodule formation was visualized by microscopy and calcium content was assessed quantitatively by alizarin red assay. Results : Experimental cultures produced an increase in cell proliferation. Immunolabeling for OPN and ALP in hPDLF were increased and ALP activity was inhibited by three-dimensional conditions. OPN and RUNX2 gene expression was significantly higher on 3D culture when compared with control surface. Moreover, ALP and COL I gene expression were significantly higher in three-dimensional collagen than in 2D cultures at 7 days. However, at 14 days, 3D cultures exhibited ALP and COL I gene expression significantly lower than the control, and the COL I gene expression was also significantly lower in 3D than in 2D cultures. Significant calcium mineralization was detected and quantified by alizarin red assay, and calcified nodule formation was not affected by tridimensionality. Conclusion : This study suggests that the 3D cultures are able to support hPDLF proliferation and favor the differentiation and mineralized matrix formation, which may be a potential periodontal regenerative therapy

Citogenética Comparativa das Famílias Leptodactylidae e Hylidae do Cerrado Goiano

Resumo: este estudo descreve a diversidade citogenética de anfíbios anuros com ênfase nas famílias Hylidae e Leptodactylidae de diferentes localidades do cerrado goiano. Foram analisadas 10 diferentes espécies coletadas nos municípios de Rio Verde, Bela Vista, Serranópolis, Barro Alto, Pirenópolis e Nazário. Foram obtidos cariótipos com número diplóide variando de 2n=22 a 2n=24. Marcações para Regiões Organizadoras de Nucléolo foram obtidas no cromossomo 8 da espécie Leptodactylus fuscus. A grande variação cromossômica pode ser explicada pelo padrão reprodutivo diversificado destes animais. Palavras-chave: Citogenética. Anfíbios. Cariótipo. Cerrado. Nucléolo

Parasite load in the blood and skin of dogs naturally infected by Leishmania infantum is correlated with their capacity to infect sand fly vectors

AbstractThe sand fly Lutzomyia longipalpis is primarily responsible for the transmission of visceral leishmaniasis (VL) in the New World, and dogs are considered to be the main urban reservoir of this disease. In order to improve the efficacy of control measures, it is essential to assess the transmission capacity of Leishmania infantum to the sand fly vector by naturally infected dogs. The present study investigated the existence of correlations between canine clinical presentation and the intensity of parasite load in the blood, skin and spleen of naturally infected dogs. In addition, we also attempted to establish correlations between the intensity of parasite load in canine tissue and the parasite load detected in sandflies five days after feeding on naturally infected dogs. A total of 23 dogs were examined and classified according to clinical manifestation of canine VL. Blood samples, splenic aspirate and skin biopsies were collected and parasite DNA was quantified by qPCR. Canine capacity to infect Lu. longipalpis with parasites was evaluated by xenodiagnosis and parasite loads were measured five days after feeding. No significant differences were observed with respect to canine clinical manifestation and the parasite loads detected in the blood, skin and spleen samples obtained from naturally infected dogs. Regardless of clinical canine visceral leishmaniasis (CVL) presentation and the degree of parasite burden, almost half of the dogs successfully infected sandflies with parasites, albeit to a low number of sandflies with correspondingly low parasite loads. Parasite loads in both canine blood and skin were shown to be positively correlated with the canine infectiousness to the sand fly vector, and positive correlations were also observed with respect to these tissues and the sand fly infection rate, as well as the parasite load detected in sandflies following xenodiagnosis. In conclusion, this indicates that parasite loads in both blood and skin can function as potentially reliable markers of canine capacity to infect sand fly vector

Ascorbate-glutathione cycle genes families in Euphorbiaceae : characterization and evolutionary analysis

Ascorbate peroxidase (APX), Monodehydroascorbate Reductase (MDAR), Dehydroascorbate Reductase (DHAR) and Glutathione Reductase (GR) enzymes participate in the ascorbateglutathione cycle, which exerts a central role in the antioxidant metabolism in plants. Despite the importance of this antioxidant system in different signal transduction networks related to development and response to environmental stresses, the pathway has not yet been comprehensively characterized in many crop plants. Among different eudicotyledons, the Euphorbiaceae family is particularly diverse with some species highly tolerant to drought. Here the APX, MDAR, DHAR, and GR genes in Ricinus communis, Jatropha curcas, Manihot esculenta, and Hevea brasiliensis were identified and characterized. The comprehensive phylogenetic and genomic analyses allowed the classification of the genes into different classes, equivalent to cytosolic, peroxisomal, chloroplastic, and mitochondrial enzymes, and revealed the duplication events that contribute to the expansion of these families within plant genomes. Due to the high drought stress tolerance of Ricinus communis, the expression patterns of ascorbate-glutathione cycle genes in response to drought were also analyzed in leaves and roots, indicating a differential expression during the stress. Altogether, these data contributed to the characterization of the expression pattern and evolutionary analysis of these genes, filling the gap in the proposed functions of core components of the antioxidant mechanism during stress response in an economically relevant group of plants