Efecto de los descansos activos físicos en la comprensión lectora de la lengua extranjera

This work is funded by General Directorate of Teacher Training and Educational Innovation of the Andalusian Ministry of Education (Resolution: august 31, 2020) under the project “The inclusion of physical activity for foreign language learning in the CLIL classroom through the learning-HITT program”.The aim of the present study was to find out whether Active-Breaks (AB) improve the three levels of reading comprehension in a foreign language which could be a determining variable in the academic success of the implementation of CLIL programmes. This study involved a total of 50 healthy students [n=25: Control Condition (CC), and n= 25: Active-Break Condition (ABC)] from one secondary school in the Region of Andalucía (Spain). To investigate the acute effects of an AB on reading comprehension in a foreign language, those in the CC were asked to maintain their ordinary routines, while those in the ABC modified their class by introducing ten minutes of exercise. After that both groups complete a reading comprehension test. Significant different were found in both question of literal comprehension, p=0.03, d=0.38, p=0.001, d=0.11, and in both question of inferential comprehension, p=.003, d=0.35, p=0.03, d=0.47. Crucially, in both question of evaluation, p=0.26, d=0.20, p=0.41, d=0.40, no significant difference was appreciated. The present study demonstrated that overall student’s reading comprehension in a foreign language improves after of AB of 10 min (compared with CC) and concretely, improve the efficiency in question of literal comprehension and inferential comprehension, contributing to enhancement of quality of education.El objetivo de la presente investigación fue el de averiguar si los descansos activos mejoran los niveles de comprensión lectora en una lengua extranjera, lo que podría ser una variable determinante en el éxito académico en la implementación de los programas AICLE. En este estudio participaron un total de 50 estudiantes sanos (n=25: Condición Control, and n= 25: Condición descanso activo) de un instituto de secundaria de Andalucía (España). Para investigar los efectos agudos de un descanso activo en la comprensión de lectura en un idioma extranjero, se les pidió a los estudiantes de Condición control que mantuvieran sus rutinas diarias, mientras que a los de la condición de descanso activo modificaron su clase introduciendo 10 minutos de ejercicio. Después de eso, ambos grupos completaron una prueba de comprensión lectora. Se encontraron diferencias significativas tanto en las preguntas de comprensión literal, p=0.03, d=0.38, p=0.001, d=0.11, como en las preguntas de comprensión inferencia, p=.003, d=0.35, p=0.03, d=0.47. Hay que destacar que, en ambas cuestiones de evaluación, p=0.26, d=0.20, p=0.41, d=0.40, no se apreciaron diferencias significativas. El presente estudio demostró que la comprensión lectora general en un idioma extranjero mejoró después de un descanso activo de 10 minutos en comparación con el Grupo Control y en concreto, mejoró de manera eficiente en términos de comprensión literal e inferencial, contribuyendo a mejorar la calidad de la educación.Andalusian Ministry of Education (Resolution: august 31, 2020

Efecto de los descansos activos físicos en la comprensión lectora de la lengua extranjera

The aim of the present study was to find out whether Active-Breaks (AB) improve the three levels of reading comprehension in a foreign language which could be a determining variable in the academic success of the implementation of CLIL programmes.  This study involved a total of 50 healthy students [n=25: Control Condition (CC), and n= 25: Active-Break Condition (ABC)] from one secondary school in the Region of Andalucía (Spain). To investigate the acute effects of an AB on reading comprehension in a foreign language, those in the CC were asked to maintain their ordinary routines, while those in the ABC modified their class by introducing ten minutes of exercise. After that both groups complete a reading comprehension test. Significant different were found in both question of literal comprehension, p=0.03, d=0.38, p=0.001, d=0.11, and in both question of inferential comprehension, p=.003, d=0.35, p=0.03, d=0.47. Crucially, in both question of evaluation, p=0.26, d=0.20, p=0.41, d=0.40, no significant difference was appreciated. The present study demonstrated that overall student’s reading comprehension in a foreign language improves after of AB of 10 min (compared with CC) and concretely, improve the efficiency in question of literal comprehension and inferential comprehension.El objetivo de la presente investigación fue el de averiguar si los descansos activos mejoran los niveles de comprensión lectora en una lengua extranjera, lo que podría ser una variable determinante en el éxito académico en la implementación de los programas AICLE. En este estudio participaron un total de 50 estudiantes sanos (n=25: Condición Control, and n= 25: Condición descanso activo) de un instituto de secundaria de Andalucía (España). Para investigar los efectos agudos de un descanso activo en la comprensión de lectura en un idioma extranjero, se les pidió a los estudiantes de Condición control que mantuvieran sus rutinas diarias, mientras que a los de la condición de descanso activo modificaron su clase introduciendo 10 minutos de ejercicio. Después de eso, ambos grupos completaron una prueba de comprensión lectora. Se encontraron diferencias significativas tanto en las preguntas de comprensión literal, p=0.03, d=0.38, p=0.001, d=0.11, como en las preguntas de comprensión inferencia, p=.003, d=0.35, p=0.03, d=0.47.  Hay que destacar que, en ambas cuestiones de evaluación, p=0.26, d=0.20, p=0.41, d=0.40, no se apreciaron diferencias significativas. El presente estudio demostró que la comprensión lectora general en un idioma extranjero mejoró después de un descanso activo de 10 minutos

Highly selective and sensitive fluorescent determination of Fe3+ within alcoholic beverages with 1,5-diphenylcarbazone-functionalized graphene quantum dots

Herein, we report the synthesis of graphene quantum dots (GQDs) functionalized with 1,5-diphenylcarbazone for the selective quantification of Fe in wine, via front-face fluorescence. Crystalline GQDs are obtained via a clean and relatively size-controlled synthesis based on the electrochemical exfoliation from a graphene foam. The product of the synthesis was later functionalized to solely detect Fe3+ amongst various other ions present within the sample. The detection is based on quenching the fluorescence emission from the functionalized nanomaterial in the presence of the analyte, which follows a linear relationship with the concentration of the analyte, consistent with the Stern-Volmer model. Diverse parameters involved in the measurements, including the pH and excitation wavelength, were optimized, giving place to limits of detection (LOD) of 0.014 mg L−1 and 0.11 mg L−1 in waters and white wines, respectively. A soft UV-based digestion and a profound analysis of interferences were key factors to achieve such LODs. Furthermore, front-face fluorescence measurements improved the applicability of the method by avoiding the commonly occurring matrix shielding effects. We believe that the sensitive, selective, and fast detection of Fe3+ within real (i.e.; wine) samples represents a major step forward in the field.Fil: Llaver, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Laboratorio de Química Analítica para Investigación y Desarrollo; ArgentinaFil: Barrionuevo, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Troiani, Horacio Esteban. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Wuilloud, Rodolfo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Laboratorio de Química Analítica para Investigación y Desarrollo; ArgentinaFil: Ibañez, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentin

Medición de la motivación para aprender inglés como lengua extranjera: Validación del LLOS-IEA. Diferencias de Sexo y Edad en las regulaciones emocionales

El objetivo de este estudio ha sido traducir al español, adaptar a un contexto AICLE y analizar las propiedades psicométricas de la escala LLOS-IEA; Escala de la orientación del aprendizaje de idiomas – Subescalas de Motivación Intrínseca, Motivación Extrínseca y desmotivación. La versión adaptada del LLOS-IEA fue administrada a 3355 estudiantes de Andalucía con edades comprendidas entre los 11 y los 17 años. Tras los procesos de traducción y adaptación, se realizaron análisis de los ítems y de la estructura interna con el fin de comparar los modelos de 5 y 7 factores. Esto dio lugar a análisis CFA, fiabilidad y validez que concluyeron el mejor ajuste del modelo de 5 factores. También se realizó un análisis de modelos multiniveles para estudiar la validez de constructo obteniendo resultados similares a otros estudios.The object of this research was to translate into Spanish and adapt to a CLIL context the Language Learning Orientation Scale-Intrinsic Motivation, Extrinsic Motivation and Amotivation Subscales (LLOS-IEA) analysing its psychometric properties. The LLOS-IEA adaptation was administered to a total of 3355 students from Andalusia aged from 11 to 17 years. After the translation and adaptation processes, item and internal structure analyses were conducted comparing the 5 and 7-factor models. This led to a CFA and to reliability and validity analyses, which concluded that the 5-factor performed the best fit. Other analyses have shown this instrument to be sex-invariant and to have convergent validity. A multi-level model analysis was also conducted in order to study the construct validity concluding with similar results from similar studies

Sertoli cell-specific ablation of miR-17-92 cluster significantly alters whole testis transcriptome without apparent phenotypic effects

MicroRNAs are frequently organized into polycistronic clusters whose transcription is controlled by a single promoter. The miR-17-92 cluster is expressed in most embryonic and postnatal organs. It is a potent oncogene associated to several types of cancer and it is involved in several important developmental processes. In the testis, expression of the miR- 17-92 cluster in the germ cells is necessary to maintain normal spermatogenesis. This cluster is also expressed in Sertoli cells (the somatic cells of the seminiferous tubules), which require miRNAs for correct cell development and survival. To study the possible role of miR- 17-92 in Sertoli cell development and function and, in order to overcome the postnatal lethality of miR-17-92-/ mice, we conditionally deleted it in embryonic Sertoli cells shortly after the sex determination stage using an Amh-Cre allele. Mutant mice developed apparently normal testes and were fertile, but their testis transcriptomes contained hundreds of moderately deregulated genes, indicating that testis homeostasis is tightly controlled in mammals and that miR-17-92 expression in Sertoli cells contribute to maintain normal gene expression levels, but is unnecessary for testis development and function. Our results show that significant deregulation of hundreds of genes might have no functional consequences.This work was supported by grants from the Andalusian Government, Junta de Andalucía, BIO-109 to R. Jiménez and P11-CVI-7291 to M. Burgos and grants from the Spanish Ministry of Science and Innovation (CGL2011-23368 and CGL2015-67108-P) to R. Jiménez and F.J. Barrionuevo. The authors would like to thank the Spanish Ministry of Science and Innovation for the 'Ramón y Cajal' fellowship granted to F.D. Carmona (RYC-2014-16458) and the 'FPU' PhD fellowship granted to A. Hurtado

Disposición a comunicarse en un idioma extranjero en las cuatro destrezas. Diferencias de edad y sexo

The object of this research was to validate the Willingness to communicate in a foreign language in the four skills in the Classroom Scale to a Spanish speaking context. Taking the study of authors (in press) as a starting point, this instrument validation will allow to obtain information about the student FLL motivation in CLIL in regular schools. This would allow researchers and teachers to have a valid instrument that provides information about the four language skills in FLL or CLIL programmes. In addition, as part of the construct validity analysis, sex and age differences are explored in order to obtain a student profile regarding Willingness to communicate in a foreign language in the four skills. The WTC-FL in the four skills adaptation was administered to a total of 3355 students from Andalusia aged from 11 to 17 years. After the translation and adaptation processes, item and internal structure analyses were conducted. Other analyses have shown this instrument to be sex-invariant and to have convergent validity. A multi-level model analysis was also conducted in order to study the construct validity, concluding with similar results from similar studies

Interleukin 27 could be useful in the diagnosis of tuberculous pleural effusions

BACKGROUND: The diagnosis of tuberculous pleural effusion (TBPE) has some limitations. We studied the efficacy of interleukin-27 (IL-27) in the diagnosis of TBPE. METHODS: We measured IL-27, adenosine deaminase (ADA), ADA-2, interferon-gamma (IFNgamma), and the ADA.IL-27 and ADA-2.IL-27 products in all the pleural effusion fluids. The diagnostic yield of IL-27 was evaluated with receiver operating characteristic curves. RESULTS: Of 431 pleural effusions, 70 were tuberculous, 146 were neoplastic, 58 were parapneumonic, 28 were empyemas, 88 were transudates, and 41 were other types. With a cutoff point of 0.55 ng/mL, IL-27 had a sensitivity of 91.4% and a specificity of 85.1%, which were significantly less than ADA, ADA-2, IFNgamma, ADA.IL-27, or ADA-2.IL-27. The area under the receiver operating characteristic curve for IL-27 (0.963) was also significantly lower than that for the other markers, except for IFNgamma. However, IL-27 improved the sensitivity of ADA and ADA-2 through ADA.IL-27 and ADA-2.IL-27 products (100% for both). CONCLUSIONS: IL-27 is less efficient than ADA and ADA-2 in the diagnosis of TBPE. However, ADA.IL-27 and ADA-2.IL-27 improve the diagnostic sensitivity of ADA and ADA-2, and thus could be useful in situations of high clinical suspicion and low ADA level. A value above the cutoff point of the latter is practically diagnostic of TBPE

Evaluation of male fertility-associated loci in a european population of patients with severe spermatogenic impairment

Funding: This work was supported by the Spanish Ministry of Economy and Competitiveness through the Spanish State Plan for Scientific and Technical Research and Innovation (ref. SAF2016-78722-R), the “Ramón y Cajal” program (ref. RYC-2014-16458), and the “Juan de la Cierva Incorporación” program (ref. IJC2018-038026-I), which include FEDER funds. SLa received support from the Spanish Ministry of Science and Innovation (grants FIS-ISCIII DTS18/00101, co-funded by FEDER funds/European Regional Development Fund (ERDF)-a way to build Europe-), and from Generalitat de Catalunya (grant 2017SGR191). AG-J was recipient of a grant from the “Plan Propio” program of the University of Granada (“Becas de Iniciación a la Investigación para estudiantes de Grado”, conv.2019). SLa is sponsored by the “Researchers Consolidation Program” from the SNS-Dpt. Salut Generalitat de Catalunya (Exp. CES09/020). JG was partially funded by FCT/MCTES, through national funds attributed to Center for Toxicogenomics and Human Health—ToxOmics (UIDB/00009/2020). PIM is supported by the FCT post-doctoral fellowship (SFRH/BPD/120777/2016), financed from the Portuguese State Budget of the Ministry for Science, Technology and High Education and from the European Social Fund, available through the Programa Operacional do Capital Humano. AML is funded by the Portuguese Government through FCT (IF/01262/2014). IPATIMUP integrates the i3S Research Unit, which is partially supported by FCT in the framework of the project “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274).Infertility is a growing concern in developed societies. Two extreme phenotypes of male infertility are non-obstructive azoospermia (NOA) and severe oligospermia (SO), which are characterized by severe spermatogenic failure (SpF). We designed a genetic association study comprising 725 Iberian infertile men as a consequence of SpF and 1058 unaffected controls to evaluate whether five single-nucleotide polymorphisms (SNPs), previously associated with reduced fertility in Hutterites, are also involved in the genetic susceptibility to idiopathic SpF and specific clinical entities. A significant difference in the allele frequencies of USP8-rs7174015 was observed under the recessive model between the NOA group and both the control group (p = 0.0226, OR = 1.33) and the SO group (p = 0.0048, OR = 1.78). Other genetic associations for EPSTI1-rs12870438 and PSAT1-rs7867029 with SO and between TUSC1-rs10966811 and testicular sperm extraction (TESE) success in the context of NOA were observed. In silico analysis of functional annotations demonstrated cis-eQTL effects of such SNPs likely due to the modification of binding motif sites for relevant transcription factors of the spermatogenic process. The findings reported here shed light on the molecular mechanisms leading to severe phenotypes of idiopathic male infertility, and may help to better understand the contribution of the common genetic variation to the development of these conditions.publishersversionpublishe

Expresión génica durante el desarrollo gonadal de talpa occidentalis

Las hembras del topo iberico, Talpa occidentalis, presentan un caso de reversion sexual, ya que en lugar de ovarios normales presentan ovotestes bilaterales, con una region con caracteristcas de tejido ovarico normal y otra region de tamaño variable, con caracteristicas de tejido testicular disgenesico. Los testiculos masculinos son invariablemente normales. Dada las caracteristicas tan excepcionales que presentan estas hembras, en el presente trabajo se ha realizado el estudio de la organogenesis gonadal en esta especie, asi como la expresion de genes implicados en la diferenciacion determinacion sexual. Para ello hemos tenido que desarrollar tecnicas para la obtencion de embriones, crias y fetos de topo, asi como para el mantenimiento de estos animales en alojamientos artificiales. Asi, hemos comprobado que el desarrollo testicular de los machos del topo Talpa occidentalis se realiza según el patron comun en otras especies de mamiferos, y los genes SRY, DAXI y SOX9 se expresan de forma ininterrumpida durante todo el desarrollo prenatal de los testiculos XY de topo, un perfil de expresion bastante distinto del de especies tales como rata y raton. La expresion continuada de SRY explica que la de de DAXI no tenga ninguna consecuencia negativa en el desarrollo testicular. El topo es el unico mamifero en el que el primer signo de diferenciacion sexual aparece en las hembras, en lugar de en los machos, ya que en un estudio precoz se observa un septo de tejido que defiine una region cortical y otra medular, cosa que no ocurre en el macho del mismo estadio. El desarrollo del tejido ovarico de los ovotestes de las hembras de topo tiene un desarrollo normal al compararlo con otras especies de mamiferos. El tejido testicular de los ovotestes del topo es ontogeneticamente homologo del de los testiculos de los machos, y se desarrolla siguiendo un patron identico al del tejido testicular XY, aunque una velocidad inferior. En consecuencia, estos animales son hermafroditas verdaderos desde un punto de vista fenotipico. La presencia del tejido testicular XX en estas gonadas permite hablar de reversion sexual XX en las especies del genero Talpa. La expresion del gen SOX9 durante todo el proceso de formacion del tejido testicular de los ovotestes de las hembras de topo apunta hacia este gen como el responsable de la induccion del desarrollo testicular. La presencia de celulas germinales primordiales XX en la region cortical, pero no en la region medular de la gonada femenina de topo desde los primeros estadios del desarrollo, sugiere que la presencia de estas celulas germinales primordiales XX en la region cortical, pero no en la region medular de la gonada femenina de topo desde los primeros estadios del desarrollo, sugiere que la presencia de estas celulas conlleva la represion, en lascelulas preSertoli, de genes necesarios para el desarrollo testicular. Entre estos genes estaria SOX9. El patron de expresion de DAXI en las gonadas XX de topo sugiere que, en ausencia de SRY, DAXI es capaz de reprimir en las celulas pre-Sertoli su capacidad para indcir la diferenciacion de las celulas de leydig, pero no la de inducir la migracion, la formacion de cordones, la vascularizacion y la formacion de la tunica albugineaUniversidad de Granada, Departamento de Genética. Leída 21-12-0