Active-Region Tilt Angles: Magnetic Versus White-Light Determinations of Joy's Law

The axes of solar active regions are inclined relative to the east--west direction, with the tilt angle tending to increase with latitude ("Joy's law"). Observational determinations of Joy's law have been based either on white-light images of sunspot groups or on magnetograms, where the latter have the advantage of measuring directly the physically relevant quantity (the photospheric field), but the disadvantage of having been recorded routinely only since the mid-1960s. White-light studies employing the historical Mount Wilson (MW) database have yielded tilt angles that are smaller and that increase less steeply with latitude than those obtained from magnetic data. We confirm this effect by comparing sunspot-group tilt angles from the Debrecen Photoheliographic Database with measurements made by Li and Ulrich using MW magnetograms taken during cycles 21--23. Whether white-light or magnetic data are employed, the median tilt angles significantly exceed the mean values, and provide a better characterization of the observed distributions. The discrepancy between the white-light and magnetic results is found to have two main sources. First, a substantial fraction of the white-light "tilt angles" refer to sunspots of the same polarity. Of greater physical significance is that the magnetograph measurements include the contribution of plage areas, which are invisible in white-light images but tend to have greater axial inclinations than the adjacent sunspots. Given the large uncertainties inherent in both the white-light and the magnetic measurements, it remains unclear whether any systematic relationship exists between tilt angle and cycle amplitude during cycles 16--23.Comment: 35 pages, 13 figures, Accepted in Ap

Analysis of low Reynolds number flow around a heated circular cylinder

The objective of this study is to investigate the forced convection from and the flow around a heated cylinder. Experimental and computational results are presented for laminar flow around a heated circular cylinder with a diameter of 10 mm. The experiments were carried out using Particle Image Velocimetry (PIV) in a wind tunnel, and numerical simulations using an in-house code and a commercial software package, FLUENT. This paper pre-sents comparisons for vorticity and temperature contours in the wake of the cylinder. Experimental and computa-tional results are compared with those available in the literature for heated and unheated cylinders. An equation is suggested for a temperature-dependent coefficient defining a reference temperature to be used in place of the con-stant used in other studies. An attempt is also made to correct differences between average cylinder surface tem-perature and measured interior temperature of the cylinder

Incorporating prior knowledge improves detection of differences in bacterial growth rate

BACKGROUND: Robust statistical detection of differences in the bacterial growth rate can be challenging, particularly when dealing with small differences or noisy data. The Bayesian approach provides a consistent framework for inferring model parameters and comparing hypotheses. The method captures the full uncertainty of parameter values, whilst making effective use of prior knowledge about a given system to improve estimation. RESULTS: We demonstrated the application of Bayesian analysis to bacterial growth curve comparison. Following extensive testing of the method, the analysis was applied to the large dataset of bacterial responses which are freely available at the web-resource, ComBase. Detection was found to be improved by using prior knowledge from clusters of previously analysed experimental results at similar environmental conditions. A comparison was also made to a more traditional statistical testing method, the F-test, and Bayesian analysis was found to perform more conclusively and to be capable of attributing significance to more subtle differences in growth rate. CONCLUSIONS: We have demonstrated that by making use of existing experimental knowledge, it is possible to significantly improve detection of differences in bacterial growth rate

The impact of neighbourhood crime on mental health: a systematic review and meta-analysis

Background: Growing evidence indicates that the residential neighbourhood contributes to the complex aetiology of mental disorders. Although local crime and violence, key neighbourhood stressors, may be linked to mental health through direct and indirect pathways, studies are inconclusive. This systematic review and meta-analysis aimed to synthetize the evidence on the association between neighbourhood crime and individual-level mental health problems. Method: We searched 11 electronic databases, grey literature and reference lists to identify relevant studies published before September 14, 2020. Studies were included if they reported confounder-adjusted associations between objective or perceived area-level crime and anxiety, depression, psychosis or psychological distress/internalising symptoms in non-clinical samples. Effect measures were first converted into Fisher's z-s, pooled with three-level random-effects meta-analyses, and then transformed into Pearson's correlation coefficients. Univariate and multivariate mixed-effects models were used to explore between-study heterogeneity. Results: We identified 63 studies reporting associations between neighbourhood crime and residents’ mental health. Pooled associations were significant for depression (r = 0.04, 95% CI 0.03–0.06), psychological distress (r = 0.04, 95% CI 0.02–0.06), anxiety (r = 0.05, 95% CI 0.01–0.10), and psychosis (r = 0.04, 95% CI 0.01–0.07). Moderator analysis for depression and psychological distress identified stronger associations with perceived crime measurement and weaker in studies adjusted for area-level deprivation. Importantly, even after accounting for study characteristics, neighbourhood crime remained significantly linked to depression and psychological distress. Findings on anxiety and psychosis were limited due to low number of included studies. Conclusions: Neighbourhood crime is an important contextual predictor of mental health with implications for prevention and policy. Area-based crime interventions targeting the determinants of crime, prevention and service allocation to high crime neighbourhoods may have public mental health benefits. Future research should investigate the causal pathways between crime exposure and mental health, identify vulnerably groups and explore policy opportunities for buffering against the detrimental effect of neighbourhood stressors.Fil: Baranyi, Gergö. University of Edinburgh; Reino UnidoFil: Di Marco, Martín Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Sociales. Instituto de Investigaciones "Gino Germani"; ArgentinaFil: Russ, Tom C.. University of Edinburgh; Reino UnidoFil: Dibben, Chris. University of Edinburgh; Reino UnidoFil: Pearce, Jamie. University of Edinburgh; Reino Unid

Treg-Therapy Allows Mixed Chimerism and Transplantation Tolerance Without Cytoreductive Conditioning

Establishment of mixed chimerism through transplantation of allogeneic donor bone marrow (BM) into sufficiently conditioned recipients is an effective experimental approach for the induction of transplantation tolerance. Clinical translation, however, is impeded by the lack of feasible protocols devoid of cytoreductive conditioning (i.e. irradiation and cytotoxic drugs/mAbs). The therapeutic application of regulatory T cells (Tregs) prolongs allograft survival in experimental models, but appears insufficient to induce robust tolerance on its own. We thus investigated whether mixed chimerism and tolerance could be realized without the need for cytoreductive treatment by combining Treg therapy with BM transplantation (BMT). Polyclonal recipient Tregs were cotransplanted with a moderate dose of fully mismatched allogeneic donor BM into recipients conditioned solely with short-course costimulation blockade and rapamycin. This combination treatment led to long-term multilineage chimerism and donor-specific skin graft tolerance. Chimeras also developed humoral and in vitro tolerance. Both deletional and nondeletional mechanisms contributed to maintenance of tolerance. All tested populations of polyclonal Tregs (FoxP3-transduced Tregs, natural Tregs and TGF-β induced Tregs) were effective in this setting. Thus, Treg therapy achieves mixed chimerism and tolerance without cytoreductive recipient treatment, thereby eliminating a major toxic element impeding clinical translation of this approach

Paracatenula, an ancient symbiosis between thiotrophic Alphaproteobacteria and catenulid flatworms

Harnessing chemosynthetic symbionts is a recurring evolutionary strategy. Eukaryotes from six phyla as well as one archaeon have acquired chemoautotrophic sulfur-oxidizing bacteria. In contrast to this broad host diversity, known bacterial partners apparently belong to two classes of bacteria—the Gamma- and Epsilonproteobacteria. Here, we characterize the intracellular endosymbionts of the mouthless catenulid flatworm genus Paracatenula as chemoautotrophic sulfur-oxidizing Alphaproteobacteria. The symbionts of Paracatenula galateia are provisionally classified as “Candidatus Riegeria galateiae” based on 16S ribosomal RNA sequencing confirmed by fluorescence in situ hybridization together with functional gene and sulfur metabolite evidence. 16S rRNA gene phylogenetic analysis shows that all 16 Paracatenula species examined harbor host species-specific intracellular Candidatus Riegeria bacteria that form a monophyletic group within the order Rhodospirillales. Comparing host and symbiont phylogenies reveals strict cocladogenesis and points to vertical transmission of the symbionts. Between 33% and 50% of the body volume of the various worm species is composed of bacterial symbionts, by far the highest proportion among all known endosymbiotic associations between bacteria and metazoans. This symbiosis, which likely originated more than 500 Mya during the early evolution of flatworms, is the oldest known animal–chemoautotrophic bacteria association. The distant phylogenetic position of the symbionts compared with other mutualistic or parasitic Alphaproteobacteria promises to illuminate the common genetic predispositions that have allowed several members of this class to successfully colonize eukaryote cells

Active-region Tilt Angles: Magnetic versus White-light Determinations of Joy's Law

The axes of solar active regions are inclined relative to the east-west direction, with the tilt angle tending to increase with latitude ("Joy's law"). Observational determinations of Joy's law have been based either on white-light images of sunspot groups or on magnetograms, where the latter have the advantage of measuring directly the physically relevant quantity (the photospheric field), but the disadvantage of having been recorded routinely only since the mid-1960s. White-light studies employing the historical Mount Wilson (MW) database have yielded tilt angles that are smaller and that increase less steeply with latitude than those obtained from magnetic data. We confirm this effect by comparing sunspot-group tilt angles from the Debrecen Photoheliographic Database with measurements made by Li and Ulrich using MW magnetograms taken during cycles 21-23. Whether white-light or magnetic data are employed, the median tilt angles significantly exceed the mean values, and provide a better characterization of the observed distributions. The discrepancy between the white-light and magnetic results is found to have two main sources. First, a substantial fraction of the white-light "tilt angles" refer to sunspots of the same polarity. Of greater physical significance is that the magnetograph measurements include the contribution of plage areas, which are invisible in white-light images but tend to have greater axial inclinations than the adjacent sunspots. Given the large uncertainties inherent in both the white-light and the magnetic measurements, it remains unclear whether any systematic relationship exists between tilt angle and cycle amplitude during cycles 16-23

Tracking the Expression of Annoyance in Call Centers

Machine learning researchers have dealt with the identification of emo- tional cues from speech since it is research domain showing a large number of po- tential applications. Many acoustic parameters have been analyzed when searching for cues to identify emotional categories. Then classical classifiers and also out- standing computational approaches have been developed. Experiments have been carried out mainly over induced emotions, even if recently research is shifting to work over spontaneous emotions. In such a framework, it is worth mentioning that the expression of spontaneous emotions depends on cultural factors, on the particu- lar individual and also on the specific situation. In this work, we were interested in the emotional shifts during conversation. In particular we were aimed to track the annoyance shifts appearing in phone conversations to complaint services. To this end we analyzed a set of audio files showing different ways to express annoyance. The call center operators found disappointment, impotence or anger as expression of annoyance. However, our experiments showed that variations of parameters derived from intensity combined with some spectral information and suprasegmental fea- tures are very robust for each speaker and annoyance rate. The work also discussed the annotation problem arising when dealing with human labelling of subjective events. In this work we proposed an extended rating scale in order to include anno- tators disagreements. Our frame classification results validated the chosen annota- tion procedure. Experimental results also showed that shifts in customer annoyance rates could be potentially tracked during phone callsSpanish Mineco under grant TIN2014- 54288-C4-4-R H2020 EU under Empathic RIA action number 769872

Spontaneous mutation accumulation in multiple strains of the green alga, Chlamydomonas reinhardtii

Estimates of mutational parameters, such as the average fitness effect of a new mutation and the rate at which new genetic variation for fitness is created by mutation, are important for the understanding of many biological processes. However, the causes of interspecific variation in mutational parameters and the extent to which they vary within species remain largely unknown. We maintained multiple strains of the unicellular eukaryote Chlamydomonas reinhardtii, for approximately 1000 generations under relaxed selection by transferring a single cell every ∼10 generations. Mean fitness of the lines tended to decline with generations of mutation accumulation whereas mutational variance increased. We did not find any evidence for differences among strains in any of the mutational parameters estimated. The overall change in mean fitness per cell division and rate of input of mutational variance per cell division were more similar to values observed in multicellular organisms than to those in other single-celled microbes. However, after taking into account differences in genome size among species, estimates from multicellular organisms and microbes, including our new estimates from C. reinhardtii, become substantially more similar. Thus, we suggest that variation in genome size is an important determinant of interspecific variation in mutational parameters

Neurochemical Changes in the Mouse Hippocampus Underlying the Antidepressant Effect of Genetic Deletion of P2X7 Receptors.

Recent investigations have revealed that the genetic deletion of P2X7 receptors (P2rx7) results in an antidepressant phenotype in mice. However, the link between the deficiency of P2rx7 and changes in behavior has not yet been explored. In the present study, we studied the effect of genetic deletion of P2rx7 on neurochemical changes in the hippocampus that might underlie the antidepressant phenotype. P2X7 receptor deficient mice (P2rx7-/-) displayed decreased immobility in the tail suspension test (TST) and an attenuated anhedonia response in the sucrose preference test (SPT) following bacterial endotoxin (LPS) challenge. The attenuated anhedonia was reproduced through systemic treatments with P2rx7 antagonists. The activation of P2rx7 resulted in the concentration-dependent release of [3H]glutamate in P2rx7+/+ but not P2rx7-/- mice, and the NR2B subunit mRNA and protein was upregulated in the hippocampus of P2rx7-/- mice. The brain-derived neurotrophic factor (BDNF) expression was higher in saline but not LPS-treated P2rx7-/- mice; the P2rx7 antagonist Brilliant blue G elevated and the P2rx7 agonist benzoylbenzoyl ATP (BzATP) reduced BDNF level. This effect was dependent on the activation of NMDA and non-NMDA receptors but not on Group I metabotropic glutamate receptors (mGluR1,5). An increased 5-bromo-2-deoxyuridine (BrdU) incorporation was also observed in the dentate gyrus derived from P2rx7-/- mice. Basal level of 5-HT was increased, whereas the 5HIAA/5-HT ratio was lower in the hippocampus of P2rx7-/- mice, which accompanied the increased uptake of [3H]5-HT and an elevated number of [3H]citalopram binding sites. The LPS-induced elevation of 5-HT level was absent in P2rx7-/- mice. In conclusion there are several potential mechanisms for the antidepressant phenotype of P2rx7-/- mice, such as the absence of P2rx7-mediated glutamate release, elevated basal BDNF production, enhanced neurogenesis and increased 5-HT bioavailability in the hippocampus