Analysis of s-triazine-degrading microbial communities in soils using most-probable-number enumeration and tetrazolium-salt detection

A simple and sensitive method for the detection and enumeration of microbial s-triazine-degrading microorganisms in soil was designed. The procedure is based on the ability of some microbes to use s-triazines, such as simazine, atrazine, and cyanuric acid, as sole nitrogen source. It employs the respiration indicator 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) to detect metabolic activity and the most-probable-number (MPN) enumeration in microtiter plates. The method was used to identify simazine- and cyanuric acid-degrading activities in agricultural soils treated with the herbicide simazine. The MPN-TTC method showed that the number of simazine- and cyanuric acid-degrading microorganisms increased four weeks after the herbicide simazine had been applied. [Int Microbiol 2007; 10(3):209-215

Isolation of dental stem cell-enriched populations from continuously growing mouse incisors

Continuous growth of the rodent incisor is enabled by epithelial and mesenchymal stem cells (ESCs and MSCs) which unceasingly replenish enamel and dentin, respectively, that wear by persistent animal gnawing. Lineage tracing studies have provided evidence that ESCs contribute to all epithelial lineages of the tooth in vivo. Meanwhile, in the mouse incisor, MSCs continuously contribute to odontoblast lineage and tooth growth. However, in vitro manipulation of ESCs has shown little progress, mainly due to lack of appropriate protocol to successfully isolate, culture, expand, and differentiate ESCs in vitro without using the co-culture system. In this chapter we describe the isolation of the Sox2-GFP+ cell population that is highly enriched in ESCs. Isolated cells can be used for various types of analyses, including in vitro culture, single cell-related analyses, etc. Furthermore, we describe ways to obtain populations enriched in the incisor MSCs using FACS sorting of antibody-labeled cells. Easily accessible FACS sorting enables easy and relatively fast isolation of the cells labeled by the fluorescent protein. © Springer Science+Business Media, LLC, part of Springer Nature 2019.Peer reviewe

Eficacia desde posiciones 1 y 2 en situación de desigualdad en el 15º Campeonato del Mundo de Waterpolo

The aim of this study was twofold: to know if the left-handed players are more effective in the goal categories and getting positive actions, in 1 and 2 position, than the right-handed in the same position at numerical inequality. To ascertain if the shots in 1 and 2 position, are less effective than the rest of the positions in the goal categories. A nomothetic observational, multidimensional and punctual design was used. The sample was the 389 shots and the 182 positive actions of 24 games (12 male and 12 female) at 15th FINA World Championship held in Barcelona. To record the data was used an ad hoc instrument for observation through the Sport Code Version Pro V9. Descriptive and Chi-square test was applied to study the relationship between variables. Left-handed players are more effective than right-handed players are if they are related to the goal and positive action categories in position 1. Shots from position 1 are the second least effective while position 2 is the fifth most effective.El objetivo de este estudio fue doble: saber si los jugadores zurdos son más efectivos en las categorías de gol y obtener acciones positivas, en la posición 1 y 2, que los diestros en la misma posición en la desigualdad numérica. Determinar si los lanzamientos desde posición 1 y 2 son menos efectivos que desde el resto de posiciones en las categorías de gol. Se utilizó un diseño nomotético observacional, multidimensional y puntual. La muestra fueron los 389 tiros y las 182 acciones positivas de 24 juegos (12 masculinos y 12 femeninos) en el 15º Campeonato Mundial FINA celebrado en Barcelona. Para registrar los datos se utilizó un instrumento ad hoc para observación a través del software Sport Code Version Pro V9. Se aplicó la prueba descriptiva y de Chi-cuadrado para estudiar la relación entre las variables. Los jugadores zurdos son más efectivos que los diestros si están relacionados con el objetivo y las categorías de acción positiva en la posición 1. Los lanzamientos desde la posición 1 son los segundos menos efectivos, mientras que desde la posición 2 es la quinta más efectiv

Life-history trait of the Mediterranean keystone species Patella rustica: growth and microbial bioerosion

The age and shell growth patterns in populations of Patella rustica of the Adriatic Sea were determined by analyzing the inner growth lines visible in shell sections. Marginal increment analysis showed annual periodicity with annual growth line being deposited in May. The growth analysis of 120 individual shells showed that 90.8 % of collected individuals were less than 4 years of age and only two individuals (1.6 %) were older than 6 years. Population structure was described and the generalized von Bertalanffy growth parameters were calculated: asymptotic length (L∞) was 38.22 mm and the growth constant (K) was 0.30 year-1. Growth performance index value of P. rustica (Ø’) was 2.64 and is among the lowest ranges reported for limpet species. Patella rustica shells were degraded to different degrees by microbial bioerosion. Microboring organisms identified were pseudofilamentous and filamentous cyanobacteria Hormathonema paulocellulare, Hyella caespitosa, Mastigocoleus testarum and Leptolyngbya sp. The overall intensity of infestation was relatively low, but increased in severity with shell length. The damage was most often restricted to the oldest parts of the shell, i.e. apex of the shell, posing difficulties in determining the exact position of the first growth line. The present study is first to introduce the use of inner growth lines in Patella rustica shell sections as a reliable method for age determination and it provides the first insight into the growth patterns of this keystone species while taking the interference of microbial shell bioerosion in consideration

An energy budget for the subtidal bivalve Modiolus barbatus (Mollusca) at different temperatures

Clearance rates, respiration rates and food absorption efficiencies of the commercially interesting subtidal bivalve Modiolus barbatus were measured at different temperatures under laboratory conditions and scope for growth calculated. Clearance rates were highest at temperatures from 20°C to 28°C, whereas respiration rate was maximal at 9°C and minimal at 26°C. Highest mean values of absorbed energy occurred at 20°C and 26°C. Scope for growth trend had negative values at 9°C, 15°C and 28°C and positive values at temperatures 20°C and 26°C. The profitable thermal window for M. barbatus to have energy sufficient for growth and reproduction corresponded to <5 months per year. Seawater temperature increases will potentially impact the ecophysiological responses of subtidal M. barbatus causing life history traits to change with important repercussions for subtidal biodiversity in the Mediterranea

Effects of a long-term adapted judo program on the health-related physical fitness of children with ASD

[Abstract] Physical fitness is one of the most important physical and mental health aspects for children with Autism Spectrum Disorder (ASD). This study aimed to test the effects of a long-term adapted judo program on the health-related physical fitness of children with ASD. The participants were recruited from various associations of families and schools for children with special needs. Twenty-one children were assigned to an experimental group and nineteen to a control group. The experimental group participated in a six-month adapted judo program consisting of 90 min of practice each week. Health-related physical fitness was measured using the indicators obtained from the ALPHA-fitness battery, the estimated VO2max and the waist/height ratio0.5. Changes within and between groups were analyzed using linear mixed models for repeated measures designs and test-retest reliability of tests requiring a maximum score using the Intraclass Correlation Coefficient (ICC). A judo program tailored for children with ASD can improve the cardio-metabolic health and cardiorespiratory fitness of its participants. The problems involved with administering physical aptitude tests that involve maximum effort or performance in children with ASD cast serious doubts on the reproducibility of their results

Exercise-based interventions to enhance long-term sustainability of physical activity in older adults: a systematic review and meta-analysis of randomized clinical trials

Older adults; Physical activity; AdherenceAdultos mayores; Actividad física; AdherenciaAdults majors; Activitat física; AdherènciaExercise is a form of physical activity (PA). PA is an important marker of health and quality of life in older adults. The purpose of this study was to conduct a systematic review of the literature to assess the effect of exercise-based interventions on an at least six-month follow up PA measure, and to describe the specific strategies implemented during the intervention to strengthen the sustainability of PA in community-dwelling 65+ year-old adults. We registered and conducted a systematic review and meta-analysis (PROSPERO: CRD42017070892) of randomized clinical trials (RCT). We searched three electronic databases during January 2018 to identify RCT assessing any type of exercise-based intervention. Studies had to report a pre-, post-, and at least 6-month post-intervention follow-up. To be included, at least one PA outcome had to be assessed. The effect of exercise-based interventions was assessed compared to active (e.g., a low-intensity type of exercise, such as stretching or toning activities) and non-active (e.g., usual care) control interventions at several time points. Secondary analyses were conducted, restricted to studies that reported specific strategies to enhance the sustainability of PA. The intervention effect was measured on self-reported and objective measures of time spent in PA, by means of standardized mean differences. Standardized mean differences of PA level were pooled. Pooled estimates of effect were computed with the DerSimonian–Laird method, applying a random effects model. The risk of bias was also assessed. We included 12 studies, comparing 18 exercise intervention groups to four active and nine non-active control groups. Nine studies reported specific strategies to enhance the long-term sustainability of PA. The strategies were mostly related to the self-efficacy, self-control, and behavior capability principles based on the social cognitive theory. Exercise interventions compared to active control showed inconclusive and heterogeneous results. When compared to non-active control, exercise interventions improved PA time at the six-months follow up (standardized mean difference (SMD) 0.30; 95%CI 0.15 to 0.44; four studies; 724 participants; I2 0%), but not at the one- or two-years follow-ups. No data were available on the mid- and long-term effect of adding strategies to enhance the sustainability of PA. Exercise interventions have small clinical benefits on PA levels in community-dwelling older adults, with a decline in the observed improvement after six months of the intervention cessation.The present study was funded by United States Department of Health & Human Services National Institutes of Health (NIH), USA, and NIH National Institute on Aging (NIA), USA, (K24 AG057728)

Efficient all-optical switching using slow light within a hollow fiber

We demonstrate a fiber-optical switch that is activated at tiny energies corresponding to few hundred optical photons per pulse. This is achieved by simultaneously confining both photons and a small laser-cooled ensemble of atoms inside the microscopic hollow core of a single-mode photonic-crystal fiber and using quantum optical techniques for generating slow light propagation and large nonlinear interaction between light beams