Effect of Changing the Vocal Tract Shape on the Sound Production of the Recorder: An Experimental and Theoretical Study

Changing the vocal tract shape is one of the techniques which can be used by the players of wind instruments to modify the quality of the sound. It has been intensely studied in the case of reed instruments but has received only little attention in the case of air-jet instruments. This paper presents a first study focused on changes in the vocal tract shape in recorder playing techniques. Measurements carried out with recorder players allow to identify techniques involving changes of the mouth shape as well as consequences on the sound. A second experiment performed in laboratory mimics the coupling with the vocal tract on an artificial mouth. The phase of the transfer function between the instrument and the mouth of the player is identified to be the relevant parameter of the coupling. It is shown to have consequences on the spectral content in terms of energy distribution among the even and odd harmonics, as well as on the stability of the first two oscillating regimes. The results gathered from the two experiments allow to develop a simplified model of sound production including the effect of changing the vocal tract shape. It is based on the modification of the jet instabilities due to the pulsating emerging jet. Two kinds of instabilities, symmetric and anti-symmetric, with respect to the stream axis, are controlled by the coupling with the vocal tract and the acoustic oscillation within the pipe, respectively. The symmetry properties of the flow are mapped on the temporal formulation of the source term, predicting a change in the even / odd harmonics energy distribution. The predictions are in qualitative agreement with the experimental observations

Method to establish average relationships among Holstein bull populations over time

Average relationship coefficients between groups of animals were calculated by repeating three calculation steps until convergence was reached. The first step was a stratified sampling of pedigrees. Second, relationships between animals in the sample were calculated, using the group relationships computed in a previous round for unknown parent relationships. Third, results were accumulated by group. Average relationship coefficients calculated in this way can be used for such purposes as to better understand the structure of a population, to more accurately calculate inbreeding coefficients, and to assign unknown parent groups using clustering methods. This method was applied to the pedigree data for the worldwide Holstein sire population. The data was derived from Interbull and North American pedigree databases. Groups were defined by year of birth, sex, and country. Average relationships between US and Canadian bulls increased across time from 0.02 in 1960 to 0.12 in 1999. The increase was continuous except for a plateau in 1992, followed by a slight decrease until 1995 when the trend to increase resumed. Average relationships across time comparing Canadian with European bulls and US with European bulls are quite similar. They increased from about 0 in 1963 to 0.10 in 1999. After a peak of 0.05 in 1966, the relationships dropped to 0.03 in 1976 before starting to increase again. For bulls born in 1996 in the US, Canada, and Germany (representing a typical European country), the average relationships were: 0.116 (CanadaCanada), 0.112 (Canada-USA), 0.1 (Canada-Germany), 0.101 (USA-USA), 0.101 (USAGermany) and 0.084 (Germany-Germany). These results demonstrate the recent dramatic increase in relationships and inbreeding in the worldwide Holstein population, and show the power of this method of calculating relationship coefficients

Irreversible Adsorption from Dilute Polymer Solutions

We study irreversible polymer adsorption from dilute solutions theoretically. Universal features of the resultant non-equilibrium layers are predicted. Two cases are considered, distinguished by the value of the local monomer-surface sticking rate Q: chemisorption (very small Q) and physisorption (large Q). Early stages of layer formation entail single chain adsorption. While single chain physisorption times tau_ads are typically microsecs, for chemisorbing chains of N units we find experimentally accessible times tau_ads = Q^{-1} N^{3/5}, ranging from secs to hrs. We establish 3 chemisorption universality classes, determined by a critical contact exponent: zipping, accelerated zipping and homogeneous collapse. For dilute solutions, the mechanism is accelerated zipping: zipping propagates outwards from the first attachment, accelerated by occasional formation of large loops which nucleate further zipping. This leads to a transient distribution omega(s) \sim s^{-7/5} of loop lengths s up to a size s_max \approx (Q t)^{5/3} after time t. By tau_ads the entire chain is adsorbed. The outcome of the single chain adsorption episode is a monolayer of fully collapsed chains. Having only a few vacant sites to adsorb onto, late arriving chains form a diffuse outer layer. In a simple picture we find for both chemisorption and physisorption a final loop distribution Omega(s) \sim s^{-11/5} and density profile c(z) \sim z^{-4/3} whose forms are the same as for equilibrium layers. In contrast to equilibrium layers, however, the statistical properties of a given chain depend on its adsorption time; the outer layer contains many classes of chain, each characterized by different fraction of adsorbed monomers f. Consistent with strong physisorption experiments, we find the f values follow a distribution P(f) \sim f^{-4/5}.Comment: 18 pages, submitted to Eur. Phys. J. E, expanded discussion sectio

Rapid, room-temperature, solvent-free mechanochemical oxidation of elemental gold into organosoluble gold salts

Gold is highly valued for a wide range of commercial and technological applications but is processed exclusively through highly aggressive and toxic solvents and/or reagents, ultimately yielding water-soluble salts that are difficult to separate from inorganic reaction byproducts. As a result, development of safer, cleaner processes that would enable gold processing in non-aqueous, organic solvent is an attractive technological goal. Here, we describe a methodology that simultaneously avoids aggressive reagents and enables gold extraction into a safe organic solvent. The methodology is based on solventless, mechanochemical oxidation of metallic gold with Oxone® in the presence of tetraalkylammonium halide salts, to directly, rapidly (within 30–60 minutes) and at room temperature convert gold metal into solid salts that are immediately soluble in pure organic solvents and aqueous alcoholic media. The organosoluble gold salts are easily separated from sulfate byproducts by direct extraction into the benign solvent ethyl acetate, which is also easily recycled for re-use, providing a strategy for gold activation and dissolution without any additional reagents for purification, such as cation exchange resins, salts, or chelating agents. Besides enabling direct extraction of gold into an organic solvent, the mechanochemically obtained organosoluble gold salts can also be readily used for further syntheses, as shown here by a two-step one-pot route to prepare air- and moisture-resistant Au(i) salts, and an improved synthesis of gold nanoparticles from bulk gold

Electronic Circular Dichroism Spectroscopy of Proteins

Circular dichroism (CD) is an important spectroscopic technique that enables the characterization of protein secondary and tertiary structure. Proteins can undergo changes in their structure when they participate in processes, for example, ligand binding. CD, therefore, can be used to monitor secondary and tertiary structural changes when a protein (receptor) binds to a drug molecule (ligand).This review describes experimental studies of protein CD and theoretical and computational methods that compute spectra from structure or structure from spectra. CD is a technique that can be used to complement X-ray, NMR, and ultraviolet-visible (UV-vis) experiments on biomolecules and proteins, all of which can be assisted by molecular modeling, which has the capability of computing CD from first principles. A combination of experimental CD and molecular modeling has the capacity to greatly enhance future multi-disciplinary research to expand our knowledge of the structure, function, and dynamics of proteins

SNiP and cut: Quantifying the potential benefits of genomic selection tools for genetic fault elimination in sheep

ABSTRACT Whole genome single nucleotide polymorphism marker (SNP) technologies are rapidly being developed and could well play a role in the future selection of farmed animals. In sheep, work is being carried out to identify SNP's associated with economically important production traits and disease resistance. Stud sheep breeders routinely cull up to 30% of lambs born due to a variety of faults. Despite this culling pressure, the faults keep occurring, suggesting low heritability, recessive inheritance or incomplete penetration of fault causing genes. The objective of this paper was to identify the potential of SNP fault detection and elimination in sheep. Simulation was used to predict changes in gene frequencies and in an index of genetic merit of production traits over time when between two and 30 SNPs were used to aid selection. The SNPs are assumed to predict the presence of deleterious recessive genes. As selection pressure applied to individual or combination SNPs increased, the rate of increase in production trait genetic merit slowed down. Thus, a balance would be required between the emphasis on SNPs actively used to select against genetic faults, relative to emphasis on genetic merit. This work identified scope for substantial economic benefits from application of SNP technology for removal of faults to both stud breeders and commercial sheep farmers

Seeing ‘Where’ through the Ears: Effects of Learning-by-Doing and Long-Term Sensory Deprivation on Localization Based on Image-to-Sound Substitution

BACKGROUND: Sensory substitution devices for the blind translate inaccessible visual information into a format that intact sensory pathways can process. We here tested image-to-sound conversion-based localization of visual stimuli (LEDs and objects) in 13 blindfolded participants. METHODS AND FINDINGS: Subjects were assigned to different roles as a function of two variables: visual deprivation (blindfolded continuously (Bc) for 24 hours per day for 21 days; blindfolded for the tests only (Bt)) and system use (system not used (Sn); system used for tests only (St); system used continuously for 21 days (Sc)). The effect of learning-by-doing was assessed by comparing the performance of eight subjects (BtSt) who only used the mobile substitution device for the tests, to that of three subjects who, in addition, practiced with it for four hours daily in their normal life (BtSc and BcSc); two subjects who did not use the device at all (BtSn and BcSn) allowed assessment of its use in the tasks we employed. The impact of long-term sensory deprivation was investigated by blindfolding three of those participants throughout the three week-long experiment (BcSn, BcSn/c, and BcSc); the other ten subjects were only blindfolded during the tests (BtSn, BtSc, and the eight BtSt subjects). Expectedly, the two subjects who never used the substitution device, while fast in finding the targets, had chance accuracy, whereas subjects who used the device were markedly slower, but showed much better accuracy which improved significantly across our four testing sessions. The three subjects who freely used the device daily as well as during tests were faster and more accurate than those who used it during tests only; however, long-term blindfolding did not notably influence performance. CONCLUSIONS: Together, the results demonstrate that the device allowed blindfolded subjects to increasingly know where something was by listening, and indicate that practice in naturalistic conditions effectively improved "visual" localization performance

Field-effect transistors assembled from functionalized carbon nanotubes

We have fabricated field effect transistors from carbon nanotubes using a novel selective placement scheme. We use carbon nanotubes that are covalently bound to molecules containing hydroxamic acid functionality. The functionalized nanotubes bind strongly to basic metal oxide surfaces, but not to silicon dioxide. Upon annealing, the functionalization is removed, restoring the electronic properties of the nanotubes. The devices we have fabricated show excellent electrical characteristics.Comment: 5 pages, 6 figure

Sensory substitution information informs locomotor adjustments when walking through apertures

The study assessed the ability of the central nervous system (CNS) to use echoic information from sensory substitution devices (SSDs) to rotate the shoulders and safely pass through apertures of different width. Ten visually normal participants performed this task with full vision, or blindfolded using an SSD to obtain information regarding the width of an aperture created by two parallel panels. Two SSDs were tested. Participants passed through apertures of +0%, +18%, +35%, and +70% of measured body width. Kinematic indices recorded movement time, shoulder rotation, average walking velocity across the trial, peak walking velocities before crossing, after crossing and throughout a whole trial. Analyses showed participants used SSD information to regulate shoulder rotation, with greater rotation associated with narrower apertures. Rotations made using an SSD were greater compared to vision, movement times were longer, average walking velocity lower and peak velocities before crossing, after crossing and throughout the whole trial were smaller, suggesting greater caution. Collisions sometimes occurred using an SSD but not using vision, indicating that substituted information did not always result in accurate shoulder rotation judgements. No differences were found between the two SSDs. The data suggest that spatial information, provided by sensory substitution, allows the relative position of aperture panels to be internally represented, enabling the CNS to modify shoulder rotation according to aperture width. Increased buffer space indicated by greater rotations (up to approximately 35% for apertures of +18% of body width), suggests that spatial representations are not as accurate as offered by full vision

Modification of perceived beer bitterness intensity, character and temporal profile by hop aroma extract

The effect of hop aroma on perceived bitterness intensity, character and temporal profile of beer was investigated. A hop aroma extract was added at 3 levels (0, 245, 490 mg/L) to beers at low, medium and high bitterness. Beers were evaluated for perceived bitterness intensity, harshness, roundedness and linger by a trained panel using a rank-rating technique at each bitterness level, with and without nose clips. The use of nose clips enabled the olfactory aspect to be decoupled from taste and mouthfeel aspects of bitterness perception. Results showed significant modification of perceived bitterness in beer by hop aroma depending on the inherent level of bitter-ness. These modifications were mainly driven by olfaction – in an example of taste-aroma interactions, as well as certain tactile sensations elicited by the hop aroma extract in the oral cavity. At low bitterness, beers with hop aroma added were perceived as more bitter, and of ‘rounded’ bitterness character relative to those without hop aroma. When judges used nose clips, this effect was completely eliminated but the sample was perceived to have a ‘harsh’ bitterness character. Conversely, at high bitterness, even when nose clips were used, judges still perceived beers containing hop aroma to be more bitter. These increases in bitterness perception with nose clips indicates the stimulating of other receptors, e.g. trigeminal receptors by hop aroma extract, which in tandem with the high bitterness, cause perceptual interactions enhancing bitterness intensity and also affecting bitterness character. Bitterness character attributes such as ‘round’ and ‘harsh’ were found to significantly depend on bitterness and aroma levels, with the second level of aroma addition (245 mg/L) giving a ‘rounded’ bitterness in low bitterness beers but ‘harsh’ bitterness in high bitterness beers. The impact of aroma on temporal bitterness was also confirmed with time-intensity measurements, and found to be mostly significant at the highest level of hop aroma addition (490 mg/L) in low bitterness beers. These findings represent a significant step forward in terms of understanding bitterness flavour perception and the wider impact of hop compounds on sensory perception