Direct Base-Assisted C‒H Cyclonickelation of 6-Phenyl-2,2'-bipyridine

The organonickel complexes [Ni(Phbpy)X] (X = Br, OAc, CN) were obtained for the first time in a direct base-assisted arene C(sp2)-H cyclometalation reaction from the rather unreactive precursor materials NiX2 and HPhbpy (6-phenyl-2,2'-bipyridine) or from the versatile precursor [Ni(HPhbpy)Br2]2. Different from previously necessary C‒Br oxidative addition at Ni(0), an extended scan of reaction conditions allowed quantitative access to the title compound from Ni(II) on synthetically useful timescales through base-assisted C‒H activation in nonpolar media at elevated temperature. Optimisation of the reaction conditions (various bases, solvents, methods) identified 1:2 mixtures of acetate and carbonate as unrivalled synergetic base pairs in the optimum protocol that holds promise as a readily usable and easily tuneable access to a wide range of direct nickelation products. While for the base-assisted C‒H metalation of the noble metals Ru, Ir, Rh, or Pd, this acetate/carbonate method has been established for a few years, our study represents the leap into the world of the base metals of the 3d series

The FLC-like gene BvFL1 is not a major regulator of vernalization response in biennial beets

Many plant species in temperate climate regions require vernalization over winter to initiate flowering. Flowering Locus C (FLC) and FLC-like genes are key regulators of vernalization requirement and growth habit in winter-annual and perennial Brassicaceae. In the biennial crop species Beta vulgaris ssp. vulgaris in the evolutionarily distant Caryophyllales clade of core eudicots growth habit and bolting time are controlled by the vernalization and photoperiod response gene BTC1 and the downstream BvFT1-BvFT2 module. B. vulgaris also contains a vernalization-responsive FLC homolog (BvFL1). Here, to further elucidate the regulation of vernalization response and growth habit in beet, we functionally characterized BvFL1 by RNAi and over-expression in transgenic plants. BvFL1 RNAi neither eliminated the requirement for vernalization of biennial beets nor had a major effect on bolting time after vernalization. Over-expression of BvFL1 resulted in a moderate late-bolting phenotype, with bolting after vernalization being delayed by approximately 1 week. By contrast, RNAi-induced down-regulation of the BvFT1-BvFT2 module led to a strong delay in bolting after vernalization by several weeks. The data demonstrate for the first time that an FLC homolog does not play a major role in the control of vernalization response in a dicot species outside the Brassicaceae

Comparative Analysis of Mesenchymal Stem Cell Cultivation in Fetal Calf Serum, Human Serum, and Platelet Lysate in 2D and 3D Systems

In vitro two-dimensional (2D) and three-dimensional (3D) cultivation of mammalian cells requires supplementation with serum. Mesenchymal stem cells (MSCs) are widely used in clinical trials for bioregenerative medicine and in most cases, in vitro expansion and differentiation of these cells are required before application. Optimized expansion and differentiation protocols play a key role in the treatment outcome. 3D cell cultivation systems are more comparable to in vivo conditions and can provide both, more physiological MSC expansion and a better understanding of intercellular and cell-matrix interactions. Xeno-free cultivation conditions minimize risks of immune response after implantation. Human platelet lysate (hPL) appears to be a valuable alternative to widely used fetal calf serum (FCS) since no ethical issues are associated with its harvest, it contains a high concentration of growth factors and cytokines and it can be produced from expired platelet concentrate. In this study, we analyzed and compared proliferation, as well as osteogenic and chondrogenic differentiation of human adipose tissue-derived MSCs (hAD-MSC) using three different supplements: FCS, human serum (HS), and hPL in 2D. Furthermore, online monitoring of osteogenic differentiation under the influence of different supplements was performed in 2D. hPL-cultivated MSCs exhibited a higher proliferation and differentiation rate compared to HS- or FCS-cultivated cells. We demonstrated a fast and successful chondrogenic differentiation in the 2D system with the addition of hPL. Additionally, FCS, HS, and hPL were used to formulate Gelatin-methacryloyl (GelMA) hydrogels in order to evaluate the influence of the different supplements on the cell spreading and proliferation of cells growing in 3D culture. In addition, the hydrogel constructs were cultivated in media supplemented with three different supplements. In comparison to FCS and HS, the addition of hPL to GelMA hydrogels during the encapsulation of hAD-MSCs resulted in enhanced cell spreading and proliferation. This effect was promoted even further by cultivating the hydrogel constructs in hPL-supplemented media

Photon-induced Nucleosynthesis: Current Problems and Experimental Approaches

Photon-induced reactions play a key role in the nucleosynthesis of rare neutron-deficient p-nuclei. The paper focuses on (gamma,alpha), (gamma,p), and (gamma,n) reactions which define the corresponding p-process path. The relation between stellar reaction rates and laboratory cross sections is analyzed for photon-induced reactions and their inverse capture reactions to evaluate various experimental approaches. An improved version S_C(E) of the astrophysical S-factor is suggested which is based on the Coulomb wave functions. S_C(E) avoids the apparent energy dependence which is otherwise obtained for capture reactions on heavy nuclei. It is found that a special type of synchrotron radiation available at SPring-8 that mimics stellar blackbody radiation at billions of Kelvin is a promising tool for future experiments. By using the blackbody synchrotron radiation, sufficient event rates for (gamma,alpha) and (gamma,p) reactions in the p-process path can be expected. These experiments will provide data to improve the nuclear parameters involved in the statistical model and thus reduce the uncertainties of nucleosynthesis calculations.Comment: 13 pages, 6 figures, EPJA, accepte

СУТНІСТЬ ТА ЗНАЧЕННЯ ПОЛІТИКИ СОЦІАЛЬНИХ ІНВЕСТИЦІЙ В ДІЯЛЬНОСТІ ПІДПРИЄМСТВ

Розглянуто сутність та суб’єкти соціальних інвестицій, напрямки соціально відповідального інвестування у світовій практиці, стан російського та українського ринків соціальних інвестицій. Зроблено висновок щодо проблем та перспектив розвитку політики соціальних інвестицій на підприємствах Україні. Essence and subjects of social investments, directions of the socially responsible investing in the world practice, and the state of russian and ukrainian social investments markets are reviewed. A conclusion about problems and prospects of social investments development at ukrainian enterprises is made

Spitzer IRS Spectroscopy of Intermediate Polars: Constraints on Mid-Infrared Cyclotron Emission

We present Spitzer Infrared Spectrograph (IRS) observations of eleven intermediate polars (IPs). Spectra covering the wavelength range from 5.2 to 14 $\mu$m are presented for all eleven objects, and longer wavelength spectra are presented for three objects (AE Aqr, EX Hya, and V1223 Sgr). We also present new, moderate resolution (R $\sim$ 2000) near-infrared spectra for five of the program objects. We find that, in general, the mid-infrared spectra are consistent with simple power laws that extend from the optical into the mid-infrared. There is no evidence for discrete cyclotron emission features in the near- or mid-infrared spectra for any of the IPs investigated. If cyclotron emission is occurring in the 5.2 to 14.0 $\mu$m bandpass it constitutes less than 1% of the bolometric luminosity of any of the IPs. We discuss our results in the context of the standard model for IPs.Comment: 43 pages, including 17 figures, accepted by the Astrophysical Journa

The Seventh Data Release of the Sloan Digital Sky Survey

This paper describes the Seventh Data Release of the Sloan Digital Sky Survey (SDSS), marking the completion of the original goals of the SDSS and the end of the phase known as SDSS-II. It includes 11663 deg^2 of imaging data, with most of the roughly 2000 deg^2 increment over the previous data release lying in regions of low Galactic latitude. The catalog contains five-band photometry for 357 million distinct objects. The survey also includes repeat photometry over 250 deg^2 along the Celestial Equator in the Southern Galactic Cap. A coaddition of these data goes roughly two magnitudes fainter than the main survey. The spectroscopy is now complete over a contiguous area of 7500 deg^2 in the Northern Galactic Cap, closing the gap that was present in previous data releases. There are over 1.6 million spectra in total, including 930,000 galaxies, 120,000 quasars, and 460,000 stars. The data release includes improved stellar photometry at low Galactic latitude. The astrometry has all been recalibrated with the second version of the USNO CCD Astrograph Catalog (UCAC-2), reducing the rms statistical errors at the bright end to 45 milli-arcseconds per coordinate. A systematic error in bright galaxy photometr is less severe than previously reported for the majority of galaxies. Finally, we describe a series of improvements to the spectroscopic reductions, including better flat-fielding and improved wavelength calibration at the blue end, better processing of objects with extremely strong narrow emission lines, and an improved determination of stellar metallicities. (Abridged)Comment: 20 pages, 10 embedded figures. Accepted to ApJS after minor correction