98 research outputs found
Active Transcriptional Repression by the Rb–E2F Complex Mediates G1 Arrest Triggered by p16INK4a, TGFβ, and Contact Inhibition
AbstractRb inhibits progression from G1 to S phase of the cell cycle. It associates with a number of cellular proteins; however, the nature of these interactions and their relative significance in cell cycle regulation are still unclear. We present evidence that Rb must normally interact with the E2F family of transcription factors to arrest cells in G1, and that this arrest results from active transcriptional repression by the Rb–E2F complex, not from inactivation of E2F. Thus, a major role of E2F in cell cycle regulation is assembly of this repressor complex. We demonstrate that active repression by Rb–E2F mediates the G1 arrest triggered by TGFβ, p16INK4a, and contact inhibition
A spectroscopic look at the gravitationally lensed type Ia SN 2016geu at z=0.409
The spectacular success of type Ia supernovae (SNe Ia) in SN-cosmology is
based on the assumption that their photometric and spectroscopic properties are
invariant with redshift. However, this fundamental assumption needs to be
tested with observations of high-z SNe Ia. To date, the majority of SNe Ia
observed at moderate to large redshifts (0.4 < z < 1.0) are faint, and the
resultant analyses are based on observations with modest signal-to-noise ratios
that impart a degree of ambiguity in their determined properties. In rare cases
however, the Universe offers a helping hand: to date a few SNe Ia have been
observed that have had their luminosities magnified by intervening galaxies and
galaxy clusters acting as gravitational lenses. In this paper we present
long-slit spectroscopy of the lensed SNe Ia 2016geu, which occurred at a
redshift of z=0.409, and was magnified by a factor of ~55 by a galaxy located
at z=0.216. We compared our spectra, which were obtained a couple weeks to a
couple months past peak light, with the spectroscopic properties of
well-observed, nearby SNe Ia, finding that SN 2016geu's properties are
commensurate with those of SNe Ia in the local universe. Based primarily on the
velocity and strength of the Si II 6355 absorption feature, we find that SN
2016geu can be classified as a high-velocity, high-velocity gradient and
"core-normal" SN Ia. The strength of various features (measured though their
pseudo-equivalent widths) argue against SN 2016geu being a faint, broad-lined,
cool or shallow-silicon SN Ia. We conclude that the spectroscopic properties of
SN 2016geu imply that it is a normal SN Ia, and when taking previous results by
other authors into consideration, there is very little, if any, evolution in
the observational properties of SNe Ia up to z~0.4. [Abridged]Comment: 12 pages, 5 figures, 4 tables. Submitted to MNRAS. Comments welcome
El análisis proteómico mediante 2D-DIGE y MS/MS permite discriminar en un mismo experimento entre ratones silvestres (WT) o deficientes CD38 (CD38ko) y entre ratones afectados o no por la artritis inducida por colágeno
Comunicaciones a congreso
The host-galaxy response to the afterglow of GRB 100901A
For Gamma-Ray Burst 100901A, we have obtained Gemini-North and Very Large
Telescope optical afterglow spectra at four epochs: one hour, one day, three
days and one week after the burst, thanks to the afterglow remaining unusually
bright at late times. Apart from a wealth of metal resonance lines, we also
detect lines arising from fine-structure levels of the ground state of Fe II,
and from metastable levels of Fe II and Ni II at the host redshift (z =
1.4084). These lines are found to vary significantly in time. The combination
of the data and modelling results shows that we detect the fall of the Ni II 4
F9/2 metastable level population, which to date has not been observed. Assuming
that the population of the excited states is due to the UV-radiation of the
afterglow, we estimate an absorber distance of a few hundred pc. This appears
to be a typical value when compared to similar studies. We detect two
intervening absorbers (z = 1.3147, 1.3179). Despite the wide temporal range of
the data, we do not see significant variation in the absorption lines of these
two intervening systems.Comment: 17 pages, 9 figures. Accepted by Monthly Notices of the Royal
Astronomical Society on Jan 11th 201
A decade of GRB follow-up by BOOTES in Spain (2003-2013)
This article covers ten years of GRB follow-ups by the Spanish BOOTES
stations: 71 follow-ups providing 23 detections. Follow-ups by BOOTES-1B from
2005 to 2008 were given in the previous article, and are here reviewed,
updated, and include additional detection data points as the former article
merely stated their existence. The all-sky cameras CASSANDRA have not yet
detected any GRB optical afterglows, but limits are reported where available
Allergic hypersensitivity to cannabis in patients with allergy and illicit drug users
ProducciĂłn CientĂfica: Cannabis is the illicit drug most widely used by young people in high-income countries. Allergy symptoms have only occasionally been reported as one of the adverse health effects of cannabis use.
Objectives: To study IgE-mediated response to cannabis in drug users, atopic patients, and healthy controls.
Methods: Asthmatic patients sensitised to pollen, and all patients sensitised to tobacco, tomato and latex, considered as cross-reacting allergens, were selected from a data base of 21,582 patients. Drug users attending a drug-rehabilitation clinic were also included. Controls were 200 non-atopic blood donors. Specific IgE determination, prick tests and specific challenge with cannabis extracts were performed in patients and controls
Astrobiological field campaign to a volcanosedimentary mars analogue methane producing subsurface protected ecosystem: Imuruk Lake (Alaska)
Viking missions reported adverse conditions for life in Mars surface. High hydrogen signal obtained by Mars orbiters has increased the interest in subsurface prospection as putative protected Mars environment with life potential. Permafrost has attracted considerable interest from an astrobiological point of view due to the recently reported results from the Mars exploration rovers. Considerable studies have been developed on extreme ecosystems and permafrost in particular, to evaluate the possibility of life on Mars and to test specific automated life detection instruments for space missions. The biodiversity of permafrost located on the Bering Land Bridge National Preserve has been studied as an example of subsurface protected niche of astrobiological interest. Different conventional (enrichment and isolation) and molecular ecology techniques (cloning, fluorescence "in situ" probe hybridization, FISH) have been used for isolation and bacterial identificationThe expedition to Imuruk Lake was supported by Centro de AstrobiologĂa-INTA (Spain). The laboratory experimental procedures were supported by Grant AYA 2010–20213 “Desarrollo de TecnologĂa para la identificaciĂłn de vida de forma automática” from the Spanish Governmen
The EMT factor ZEB1 paradoxically inhibits EMT in BRAF-mutant carcinomas
Despite being in the same pathway, mutations of KRAS and BRAF in colorectal carcinomas (CRCs) determine distinct progression courses. ZEB1 induces an epithelial-to-mesenchymal transition (EMT) and is associated with worse progression in most carcinomas. Using samples from patients with CRC, mouse models of KrasG12D and BrafV600E CRC, and a Zeb1-deficient mouse, we show that ZEB1 had opposite functions in KRAS-and BRAF-mutant CRCs. In KrasG12D CRCs, ZEB1 was correlated with a worse prognosis and a higher number of larger and undifferentiated (mesenchymal or EMT-like) tumors. Surprisingly, in BrafV600E CRC, ZEB1 was associated with better prognosis; fewer, smaller, and more differentiated (reduced EMT) primary tumors; and fewer metastases. ZEB1 was positively correlated in KRAS-mutant CRC cells and negatively in BRAF-mutant CRC cells with gene signatures for EMT, cell proliferation and survival, and ERK signaling. On a mechanistic level, ZEB1 knockdown in KRAS-mutant CRC cells increased apoptosis and reduced clonogenicity and anchorage-independent growth; the reverse occurred in BRAFV600E CRC cells. ZEB1 is associated with better prognosis and reduced EMT signature in patients harboring BRAF CRCs. These data suggest that ZEB1 can function as a tumor suppressor in BRAF-mutant CRCs, highlighting the importance of considering the KRAS/BRAF mutational background of CRCs in therapeutic strategies targeting ZEB1/EMT
- …