30 research outputs found
Graphene-Based Nanomaterials for Neuroengineering: Recent Advances and Future Prospective
Graphene unique physicochemical properties made it prominent among other allotropic forms of carbon, in many areas of research and technological applications. Interestingly, in recent years, many studies exploited the use of graphene family nanomaterials (GNMs) for biomedical applications such as drug delivery, diagnostics, bioimaging, and tissue engineering research. GNMs are successfully used for the design of scaffolds for controlled induction of cell differentiation and tissue regeneration. Critically, it is important to identify the more appropriate nano/bio material interface sustaining cells differentiation and tissue regeneration enhancement. Specifically, this review is focussed on graphene-based scaffolds that endow physiochemical and biological properties suitable for a specific tissue, the nervous system, that links tightly morphological and electrical properties. Different strategies are reviewed to exploit GNMs for neuronal engineering and regeneration, material toxicity, and biocompatibility. Specifically, the potentiality for neuronal stem cells differentiation and subsequent neuronal network growth as well as the impact of electrical stimulation through GNM on cells is presented. The use of field effect transistor (FET) based on graphene for neuronal regeneration is described. This review concludes the important aspects to be controlled to make graphene a promising candidate for further advanced application in neuronal tissue engineering and biomedical use
Remote Magnetic Orientation of 3D Collagen Hydrogels for Directed Neuronal Regeneration
Hydrogel matrices are valuable platforms
for neuronal tissue engineering. Orienting gel fibers to achieve a
directed scaffold is important for effective functional neuronal regeneration.
However, current methods are limited and require treatment of gels
prior to implantation, ex-vivo, without taking into consideration
the pathology in the injured site. We have developed a method to control
gel orientation dynamically and remotely in situ. We have mixed into
collagen hydrogels magnetic nanoparticles then applied an external
magnetic field. During the gelation period the magnetic particles
aggregated into magnetic particle strings, leading to the alignment
of the collagen fibers. We have shown that neurons within the 3D magnetically
induced gels exhibited normal electrical activity and viability. Importantly,
neurons formed elongated cooriented morphology, relying on the particle
strings and fibers as supportive cues for growth. The ability to inject
the mixed gel directly into the injured site as a solution then to
control scaffold orientation remotely opens future possibilities for
therapeutic engineered scaffolds
Remote Magnetic Orientation of 3D Collagen Hydrogels for Directed Neuronal Regeneration
Hydrogel matrices are valuable platforms
for neuronal tissue engineering. Orienting gel fibers to achieve a
directed scaffold is important for effective functional neuronal regeneration.
However, current methods are limited and require treatment of gels
prior to implantation, ex-vivo, without taking into consideration
the pathology in the injured site. We have developed a method to control
gel orientation dynamically and remotely in situ. We have mixed into
collagen hydrogels magnetic nanoparticles then applied an external
magnetic field. During the gelation period the magnetic particles
aggregated into magnetic particle strings, leading to the alignment
of the collagen fibers. We have shown that neurons within the 3D magnetically
induced gels exhibited normal electrical activity and viability. Importantly,
neurons formed elongated cooriented morphology, relying on the particle
strings and fibers as supportive cues for growth. The ability to inject
the mixed gel directly into the injured site as a solution then to
control scaffold orientation remotely opens future possibilities for
therapeutic engineered scaffolds
Graphene- Based Nanomaterials for Neuroengineering: Recent Advances and Future Prospective
Graphene unique physicochemical properties made it prominent among other allotropic forms of carbon, in many areas of research and technological applications. Interestingly, in recent years, many studies exploited the use of graphene family nanomaterials (GNMs) for biomedical applications such as drug delivery, diagnostics, bioimaging, and tissue engineering research. GNMs are successfully used for the design of scaffolds for controlled induction of cell differentiation and tissue regeneration. Critically, it is important to identify the more appropriate nano/bio material interface sustaining cells differentiation and tissue regeneration enhancement. Specifically, this review is focussed on graphene- based scaffolds that endow physiochemical and biological properties suitable for a specific tissue, the nervous system, that links tightly morphological and electrical properties. Different strategies are reviewed to exploit GNMs for neuronal engineering and regeneration, material toxicity, and biocompatibility. Specifically, the potentiality for neuronal stem cells differentiation and subsequent neuronal network growth as well as the impact of electrical stimulation through GNM on cells is presented. The use of field effect transistor (FET) based on graphene for neuronal regeneration is described. This review concludes the important aspects to be controlled to make graphene a promising candidate for further advanced application in neuronal tissue engineering and biomedical use.Interactions of cells through graphene nanomaterials is presented. The article concludes that graphene is a promising candidate for neural tissue engineering.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/171044/1/adfm202104887.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/171044/2/adfm202104887_am.pd