Transcriptional regulation of wood formation in eucalyptus. Role of MYB transcription factors and protein-protein interactions

Notre objectif était de mieux comprendre la régulation de la biosynthèse des parois secondaires lors de la formation du bois chez l'Eucalyptus, le feuillu le plus planté au monde et le deuxième dont le génome est séquencé. Nous avons caractérisé trois facteurs de transcription de la famille MYB-R2R3 et montré que EgMYB137 était un nouveau régulateur de la biosynthèse des parois secondaires. Nous avons aussi démontré que l'activité transcriptionnelle de EgMYB1, un répresseur de la biosynthèse des lignines, était régulée par une interaction protéine-protéine impliquant une histone linker (EgH1.3). Enfin, nous avons mis au point une méthode de transformation homologue chez l'Eucalyptus via A. rhizogenes. Les " hairy roots " transgéniques sont adaptées à la caractérisation fonctionnelle de gènes reliés à la formation du xylème. Nos résultats ont permis de découvrir de nouveaux acteurs impliqués dans la régulation des parois secondaires, mettant en lumière la complexité de ce processus mais aussi offrant de nouvelles perspectives pour l'amélioration du bois pour des applications industrielles comme la production de bioéthanol de deuxième génération.Our objective was to better understand the regulation of the biosynthesis of the lignified secondary cell walls during wood formation in Eucalyptus, the most planted hardwood tree, and the second whose genome has been sequenced. We functionally characterized three Eucalyptus transcription factors of the R2R3-MYB family and identified EgMYB137 as a new regulator of secondary cell wall deposition. We also showed that the transcriptional activity of EgMYB1, a repressor of lignin biosynthesis was modulated by protein-protein interactions involving a linker histone (EgH1.3). Finally, we set up a homologous transformation system for Eucalyptus using Agrobacterium rhizogenes. The transgenic hairy roots are suitable for high throughput functional characterization of cell wall-related genes. Our findings not only allowed getting new insights into the complexity of the network regulating secondary cell walls but also open new avenues to improve wood quality for industrial applications such as second-generation bioethanol

Phylogenetic characterization and quantification of ammonia-oxidizing archaea and bacteria from Lake Kivu in a long-term microcosm incubation

A microcosm cultivation-based method was set up to investigate the growth of ammonia-oxidizing archaea (AOA), isolated from a water sample acquired at a depth of 50 m from the northern basin of Lake Kivu. For this purpose, both CARD-FISH and qPCR targeting of archaeal 16S rRNA and amoA genes were used. Archaeal cell growth at the end of the 246-day microcosm experiment accounted for 35 % of the SybrGold-stained cells, which corresponded to 6.61 × 106 cells/ml and 1.76 ± 0.09 × 106 archaeal 16S rRNA gene copies/ml. Clone libraries and DGGE fingerprinting confirmed the dominance of AOA phylotypes in the archaeal community microcosm. The majority of the identified archaeal 16S rRNA gene sequences in the clone libraries were affiliated with Thaumarchaeota Marine Group 1.1a. Subsequent cultivation of the AOA community on deep-well microtiter plates in medium containing different carbon sources to stimulate archaeal growth failed to show significant differences in archaeal abundance (ANOVA t14 = –1.058, P = 0.308 and ANOVA t14 = 1.584, P = 0.135 for yeast extract and simple organic acids, respectively). The lack of growth stimulation by organic compounds is in concordance with the oligotrophic status of Lake Kivu. Finally, the addition of antibiotics to the growth medium resulted in archaeal cell counts that were significantly lower than those obtained from cultures in antibiotic-free medium (ANOVA t14 = 12.12, P < 0.001). [Int Microbiol 2013; 16(3):177-189]Keywords: ammonia-oxidizing archaea and bacteria · ammonia monooxygenase alpha subunit (amoA) · Lake Kivu ·microcosm · multi-color CARD-FIS

Spot diagnosis

Question: A 40 year old male presented with a five week history of rash. His primary physician had initially treated him for eczema, without improvement

Heat identification by 17\u3b2-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay

Background: There is a substantial decline in first-service-pregnancy-rate in dairy cows. In this regard, future prospects are to measure milk hormones on-farm and progesterone levels in milk are not enough to precise ovulation unless connected to other data. The objectives of this study were to investigate whether 17\u3b2-estradiol could be measured from individual cow milk samples using a commercially available non-radiolabelled enzyme immunoassay kit (EIA) with no previously reported milk application, and whether those detections could precisely illustrate 17\u3b2-estradiol pre-ovulation peak in spite of its limited concentration and short manifestation in milk. Results: Milk sample treatments for progesterone and 17\u3b2-estradiol EIA measurements are described. Hormonal profiles from daily milk samples of six different cows were reported and 17\u3b2-estradiol pre-ovulation peak was visualized in all cases. Heat detection was possible by EIA using one every 2 days milking samples in almost all studied cases. Only in one case, morning and afternoon milking samples were required to visualize the 17\u3b2-estradiol pre-ovulation peak. Conclusions: 17\u3b2-estradiol EIA quantification in raw milk is a reliable, rapid, economic and a precise method to describe cow heat along with EIA progesterone determination

Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax)

Hydroxyproline (L-Hyp) is amply present in fishmeal but limited in plant-protein sources. This study was conducted to evaluate the effects of supplementation with dietary L-Hyp on the distribution of collagen types in the muscle, and on the texture, survival rate, growth rate, feed utilization, body composition as well as the expression of the gene that encodes the pro-alpha2 chains of type I collagen (Col Iα2) of large European sea bass (initial body weight 609.21 ± 75.39 g) from high plant-protein diets. Four isoproteic (42 % crude protein) and isolipidic (20 % crude lipid) experimental diets were formulated adding 0.6 (HL diet), 1.2 (HM diet), and 2% (HH diet) L-Hyp, respectively. Three periods of feeding of 45, 99 and 143 days were studied. L-Hyp supplementation at 1.2 % and 2 % significantly improved specific growth rate (SGR) and feed conversion ratio (FCR). In the white muscle, type I, IV collagen and trichromic stain were significantly higher in HH feed than the control diet. In the red muscle, only type I collagen was higher. HH diet, also increases Col Iα2 mRNA levels in muscle significantly. It can be concluded that the addition of crystalline l-Hyp at 1.2 % or 2 % in high plant-protein diets indicates positive effects on growth performance of adult European sea bass and increase in muscle total collagen deposition.info:eu-repo/semantics/publishedVersio

Modelos para avaliar a sustentabilidade das organizações

The complex nature of organizational sustainability has challenged researchers, scholars and international agencies and institutions when searching for a model. This is reflected in the wide variety of frameworks, methodologies and methods existing in current literature. This research study approaches the models that aim to assess enterprise sustainability relying on sustainable development principles as well as on ethical theories of social responsibility in the organizational realm. In order to achieve this goal, this paper identifies the main distinctive features and modifications introduced to each model since its emergence, which allows to classify them into three groups, and finally points out its advantages and disadvantages.La naturaleza compleja de la sostenibilidad organizacional ha desafiado a los investigadores, académicos, organismos e instituciones internacionales en la búsqueda de un modelo, muestra de esto es la gran variedad de marcos de trabajo, metodologías y métodos existentes en la literatura actual. La presente investigación abarca el estudio de los modelos para evaluar la sostenibilidad empresarial enmarcados en principios de desarrollo sostenible y teorías éticas de la responsabilidad social en el nivel empresarial. Para lograr lo anterior son identificadas las principales características distintivas y modificaciones de cada modelo desde su surgimiento, lo que permite su clasificación en tres grupos, para luego identificar sus ventajas y desventajas.A natureza complexa da sustentabilidade organizacional tem desafiado pesquisadores, acadêmicos, agências e instituições internacionais na busca de um modelo. Prova disso é a grande variedade de estruturas, metodologias e métodos existentes na literatura atual. A presente pesquisa inclui o estudo dos modelos para avaliar a sustentabilidade empresarial enquadrada em princípios de desenvolvimento sustentável e teorias éticas da responsabilidade social a nível gerencial. Para alcançar o que segue, são identificadas as principais características e mudanças de cada modelo desde a sua emergência, que permite a sua classificação em três grupos, para depois identificar suas vantagens e desvantagens

Concurs de la Ciutat Judicial

Concurs de la Ciutat JudicialPrimer premiPeer ReviewedAward-winningPostprint (published version

Molecular characterization of imported and autochthonous dengue in northeastern spain

Dengue is the most significant arbovirus worldwide and a public health threat to nonendemic areas in which Aedes vectors are present. Autochthonous dengue transmission has been reported in several European countries in the last decade. Infected travelers from endemic regions arriving to areas colonized by Aedes albopictus in Europe need to be monitored in surveillance and control programs. We aimed to perform molecular characterization of RT-PCR-positive dengue cases detected in Catalonia, northeastern Spain, from 2013 to 2018. The basic demographic information and the geographical regions of importation were also analyzed. One-hundred four dengue cases were studied (103 imported infections and the first autochthonous case in our region). The dengue virus strains detected were serotyped and genotyped using molecular methods, and phylogenetic analyses were conducted. All four dengue serotypes were detected in travelers, including up to 10 different genotypes, reflecting the global circulation of dengue in endemic areas. The primary travel-related case of the 2018 autochthonous transmission was not identified, but the molecular analysis revealed dengue serotype 1, genotype I of Asian origin. Our results highlight the diversity of imported dengue virus strains and the role of molecular epidemiology in supporting arbovirus surveillance programs

Intermediate Molecular Phenotypes to Identify Genetic Markers of Anthracycline-Induced Cardiotoxicity Risk.

Cardiotoxicity due to anthracyclines (CDA) affects cancer patients, but we cannot predict who may suffer from this complication. CDA is a complex trait with a polygenic component that is mainly unidentified. We propose that levels of intermediate molecular phenotypes (IMPs) in the myocardium associated with histopathological damage could explain CDA susceptibility, so variants of genes encoding these IMPs could identify patients susceptible to this complication. Thus, a genetically heterogeneous cohort of mice (n = 165) generated by backcrossing were treated with doxorubicin and docetaxel. We quantified heart fibrosis using an Ariol slide scanner and intramyocardial levels of IMPs using multiplex bead arrays and QPCR. We identified quantitative trait loci linked to IMPs (ipQTLs) and cdaQTLs via linkage analysis. In three cancer patient cohorts, CDA was quantified using echocardiography or Cardiac Magnetic Resonance. CDA behaves as a complex trait in the mouse cohort. IMP levels in the myocardium were associated with CDA. ipQTLs integrated into genetic models with cdaQTLs account for more CDA phenotypic variation than that explained by cda-QTLs alone. Allelic forms of genes encoding IMPs associated with CDA in mice, including AKT1, MAPK14, MAPK8, STAT3, CAS3, and TP53, are genetic determinants of CDA in patients. Two genetic risk scores for pediatric patients (n = 71) and women with breast cancer (n = 420) were generated using machine-learning Least Absolute Shrinkage and Selection Operator (LASSO) regression. Thus, IMPs associated with heart damage identify genetic markers of CDA risk, thereby allowing more personalized patient management.J.P.L.’s lab is sponsored by Grant PID2020-118527RB-I00 funded by MCIN/AEI/10.13039/ 501100011039; Grant PDC2021-121735-I00 funded by MCIN/AEI/10.13039/501100011039 and by the “European Union Next Generation EU/PRTR”, the Regional Government of Castile and León (CSI144P20). J.P.L. and P.L.S. are supported by the Carlos III Health Institute (PIE14/00066). AGN laboratory and human patients’ studies are supported by an ISCIII project grant (PI18/01242). The Human Genotyping unit is a member of CeGen, PRB3, and is supported by grant PT17/0019 of the PE I + D + i 2013–2016, funded by ISCIII and ERDF. SCLl is supported by MINECO/FEDER research grants (RTI2018-094130-B-100). CH was supported by the Department of Defense (DoD) BCRP, No. BC190820; and the National Cancer Institute (NCI) at the National Institutes of Health (NIH), No. R01CA184476. Lawrence Berkeley National Laboratory (LBNL) is a multi-program national laboratory operated by the University of California for the DOE under contract DE AC02-05CH11231. The Proteomics Unit belongs to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019/0023 of the PE I + D +i, 2017–2020, funded by ISCIII and FEDER. RCC is funded by fellowships from the Spanish Regional Government of Castile and León. NGS is a recipient of an FPU fellowship (MINECO/FEDER). hiPSC-CM studies were funded in part by the “la Caixa” Banking Foundation under the project code HR18-00304 and a Severo Ochoa CNIC Intramural Project (Exp. 12-2016 IGP) to J.J.S

Vigilància epidemiològica dels casos greus hospitalitzats confirmats de grip. Xarxa sentinella PIDIRAC (Catalunya 2010-2015)

Grip; Viigilància; Epidemiologia; Antivírics; VacunaGripe; Vigilancia; Epidemiología; Antivíricos; VacunaFlu; Surveillance; Epidemiology; Antivirals; VaccineIntroducció: el Pla d’informació de les infeccions respiratòries agudes a Catalunya (PIDIRAC) va incorporar la vigilància de casos greus hospitalitzats confirmats de grip (CGHCG) l’any 2009. L’objectiu de l’estudi és descriure les característiques clíniques, epidemiològiques i virològiques dels CGHCG registrats en 12 hospitals de la xarxa sentinella durant cinc temporades gripals. Mètode: la mostra consta dels CGHCG registrats durant les temporades que van de 2010-2011 a 2014-2015. La tècnica de confirmació emprada ha estat la PCR i/o l’aïllament viral en cultiu cel·lular a partir de mostra respiratòria. Resultats: es van registrar 1.400 CGHCG, dels quals un 33% van requerir ingrés a l’UCI i un 12% van ser èxitus. La mitjana d’edat dels casos va ser de 55,2 anys (DE: 26,7 anys), amb un rang de 0-101 anys. Un 70,8% no estaven vacunats; un 87% van rebre tractament antiviral en el 80,4% i el 24% dels casos abans de 48 hores d’ingrés i d’inici de símptomes, respectivament. En el 87,7% dels casos es va identificar el virus de la grip A (37,9% A(H1N1)pdm09 i 29,3% A(H3N2)). Conclusions: la vigilància de CGHCG proporciona una estimació de la gravetat de les epidèmies estacionals de grip i permet identificar i caracteritzar grups de risc per adoptar mesures preventives (vacunació) i tractament antiviral precoç.Introducción: el Plan de información de las infecciones respiratorias agudas en Cataluña (PIDIRAC) incorporó la vigilancia de casos graves hospitalizados confirmados de gripe (CGHCG) el año 2009. El objetivo del estudio es describir las características clínicas, epidemiológicas y virológicas de los CGHCG registrados en 12 hospitales de la red centinela durante cinco temporadas gripales. Método: la muestra consta de los CGHCG registrados durante las temporadas que van de 2010-2011 a 2014-2015. La técnica de confirmación utilizada ha sido la PCR y/o el aislamiento viral en cultivo celular a partir de muestra respiratoria. Resultados: se registraron 1.400 CGHCG, de los cuales un 33% requirieron ingreso a la UCI y un 12% fueron exitus. La media de edad de los casos fue de 55,2 años (DE: 26,7 años), con un rango de 0-101 años. Un 70,8% no estaban vacunados; un 87% recibieron tratamiento antiviral, en el 80,4% y el 24% de los casos antes de 48 horas de ingreso y de inicio de síntomas, respectivamente. En el 87,7% de los casos se identificó virus de la gripe A (37,9% A(H1N1)pdm09 y 29,3% A(H3N2)). Conclusiones: la vigilancia de CGHCG proporciona una estimación de la gravedad de las epidemias estacionales de gripe y permite identificar y caracterizar grupos de riesgo para adoptar medidas preventivas (vacunación) y tratamiento antiviral precoz.Introduction: the Information Plan for Acute Respiratory Infections in Catalonia (PIDIRAC) incorporated the surveillance of severe confirmed influenza hospitalized cases (CGHCG) in 2009. The objective of the study is to portray the clinical, epidemiological and virological features of the CGHCG registered in 12 sentinel hospitals during 5 influenza seasons. Method: the sample consists of the CGHCG registered during 2010-2011 to 2014-2015 influenza seasons. The confirmation technique used was PCR and/or viral isolation in cell culture from respiratory sample. Results: 1400 CGHCG were registered, of which 33% required admission to ICU and 12% were exitus. The mean age of cases was 55.2 years (SD: 26.7 years), range of 0-101 years. 70.8% were not vaccinated; 87% received antiviral treatment, in 80.4% and 24% of the cases before 48 hours of admission and of beginning of symptoms, respectively. 87,7% of the cases identified influenza virus A (37,9% AH1N1pdm09, 29,3% AH3N2). Conclusions: surveillance of CGHCG provides an estimation of the severity of seasonal influenza epidemics allows to identify and characterize at-risk groups to adopt preventive measures (vaccination) and early antiviral treatment