Hidden Power: Journalistic Representations of Mental Health Labels

Individuals with disabilities make up the largest minority group in the U.S., and the language used to construct representations of these individuals has the ability to perpetuate or diminish stereotypes about these individuals. The purpose of this case study was to explore and describe the representations of mental health in online newspaper articles published by three national publications – The Washington Post, The New York Times, and USA Today. Critical Discourse Analysis (CDA) was used as the methodological framework, including an analysis of semiotic choices, dominant perspectives, and causality. The case study allowed for data collection using the key terms mental health and mental illness from the three online newspapers, with a total of 33 articles published between July 1, 2018 and December 31, 2018. The findings identified that most discussions of mental health and mental illness align with a medical model frame and incorporate medicalized lexicon. Dominant perspectives of causality within articles remain with law enforcement, lawmakers, and legal advisors. Overlexicalization was evident, and the use of mental health and mental illness was more often stated with semi-formal or formal language. Findings also suggest that individuals with mental health labels are often labeled as an aggressor with specific individuals or local citizens as their victims. Potential ramifications of hidden power, as well as recommendations on altering the use of the key terms and sources used within an article are discussed

The genome of Leishmania panamensis: insights into genomics of the L. (Viannia) subgenus.

Kinetoplastid parasites of the Leishmania genus cause several forms of leishmaniasis. Leishmania species pathogenic to human are separated into two subgenera, Leishmania (Leishmania) and L. (Viannia). Species from the Viannia subgenus cause predominantly cutaneous leishmaniasis in Central and South America, occasionally leading to more severe clinical presentations. Although the genomes of several species of Leishmania have been sequenced to date, only one belongs to this rather different subgenus. Here we explore the unique features of the Viannia subgenus by sequencing and analyzing the genome of L. (Viannia) panamensis. Against a background of conservation in gene content and synteny, we found key differences at the genomic level that may explain the occurrence of molecular processes involving nucleic acid manipulation and differential modification of surface glycoconjugates. These differences may in part explain some phenotypic characteristics of the Viannia parasites, including their increased adaptive capacity and enhanced metastatic ability.Kinetoplastid parasites of the Leishmania genus cause several forms of leishmaniasis. Leishmania species pathogenic to human are separated into two subgenera, Leishmania (Leishmania) and L. (Viannia). Species from the Viannia subgenus cause predominantly cutaneous leishmaniasis in Central and South America, occasionally leading to more severe clinical presentations. Although the genomes of several species of Leishmania have been sequenced to date, only one belongs to this rather different subgenus. Here we explore the unique features of the Viannia subgenus by sequencing and analyzing the genome of L. (Viannia) panamensis. Against a background of conservation in gene content and synteny, we found key differences at the genomic level that may explain the occurrence of molecular processes involving nucleic acid manipulation and differential modification of surface glycoconjugates. These differences may in part explain some phenotypic characteristics of the Viannia parasites, including their increased adaptive capacity and enhanced metastatic ability

Análisis de propiedades de mezclas asfálticas modificadas en Panamá

This investigation proposes the determination and comparison of the technical and economic benefits that the polymers bring to asphaltic cement, by the analyzing of its performing in the asphaltic mixes against their deformation by high temperatures and loads. A laboratory study was made to determinate the asphaltic cement modification, by using polymers like: Butanol NX 1129, Muestra A1 and Elvaloy, which were added in different portions for the asphalt AC-30 that came from RefineríaTexaco/Chevron. Among the tests conducted we can mention: penetration (25 °C, 100 g, 5 s), rotational viscosity (135 °C and 175 °C), softening point and torsional elastic recovery at 25 °C. The properties of the modificate asphaltic cement were analyzed which improve significantly with polymers, in special the torsional elastic recovery. Some samples were took from the enlargement of CPA Santiago–David, because they were using polymers to modify its roads, to obtain the corresponding test tubs and compare the modified asphalt by the modulus resilient of the asphaltic mix. In addition, the volumetric behavior and Marshall parameters for modified mixtures were examined. In this evaluation it was observed that the resilient modulus of the mix is affected by the particle size, the bulk density of the mixture, unfilled spaces and that polymers reduced temperature susceptibility to deformation. Finally, an economic analysis was performed, showing that the reduction in thickness in the the AASHTO-93 design in asphalt by increasing the magnitude of the resilient modulus by addition of polymers, these results were compared used design temperatures of 25 °C and 40°C, and the minimum values and real project values of CBR.Esta investigación propone la determinación y comparación de los beneficios técnicos y económicos que aportan los polímeros al cemento asfáltico, analizando su desempeño en mezclas asfálticas frente a las deformaciones por altas temperaturas y cargas. Se realizó un estudio a nivel de laboratorio para determinar la modificación del cemento asfáltico, utilizando polímeros comercialmente conocidos como Butonal NX 1129, Muestra A1 y Elvaloy, los cuales fueron mezclados mediante aplicaciones de diferentes dosis al asfalto AC-30 proveniente de la Refinería Texaco/Chevron. Entre las pruebas realizadas al asfalto modificado están: penetración (25 °C, 100 g, 5 s), viscosidad rotacional (135 °C y 175 °C), punto de ablandamiento y recuperación elástica torsional a 25 °C. Se analizaron las propiedades del cemento asfáltico las cuales mejoran significativamente con los polímeros, en especial la recuperación elástica torsional. Tomando en cuenta que en la ampliación Santiago – David se utilizaron polímeros para modificar las rodaduras, se obtuvieron muestras de diferentes tramos para realizar las probetas correspondientes y comparar los asfaltos modificados mediante el módulo resiliente de las mezclas asfálticas modificadas. Además, se examinó el comportamiento volumétrico y los parámetros Marshall para mezclas modificadas. En esta evaluación se observó que el módulo Resiliente Ade la mezcla se ve afectado por la granulometría, la densidad aparente de la mezcla, los vacíos y los polímeros, que reducen la susceptibilidad a la temperatura y a la deformación. Por último, se realizó un análisis económico que muestra la reducción de espesores en el diseño AAST-HO-93 de la carpeta asfáltica al aumentar la magnitud del módulo resiliente mediante la adición de polímeros, se compararon dichos resultados utilizado temperaturas de diseño de 25 °C y 40 °C, y utilizando valores de CBR mínimos y los reales provenientes del proyecto ejecutado

Characterization Of Drug Interactions With Serum Proteins by Using High-Performance Affinity Chromatography

The binding of drugs with serum proteins can affect the activity, distribution, rate of excretion, and toxicity of pharmaceutical agents in the body. One tool that can be used to quickly analyze and characterize these interactions is high-performance affinity chromatography (HPAC). This review shows how HPAC can be used to study drug-protein binding and describes the various applications of this approach when examining drug interactions with serum proteins. Methods for determining binding constants, characterizing binding sites, examining drug-drug interactions, and studying drug-protein dissociation rates will be discussed. Applications that illustrate the use of HPAC with serum binding agents such as human serum albumin, α1-acid glycoprotein, and lipoproteins will be presented. Recent developments will also be examined, such as new methods for immobilizing serum proteins in HPAC columns, the utilization of HPAC as a tool in personalized medicine, and HPAC methods for the high-throughput screening and characterization of drug-protein binding

Characterization Of Drug Interactions With Serum Proteins by Using High-Performance Affinity Chromatography

The binding of drugs with serum proteins can affect the activity, distribution, rate of excretion, and toxicity of pharmaceutical agents in the body. One tool that can be used to quickly analyze and characterize these interactions is high-performance affinity chromatography (HPAC). This review shows how HPAC can be used to study drug-protein binding and describes the various applications of this approach when examining drug interactions with serum proteins. Methods for determining binding constants, characterizing binding sites, examining drug-drug interactions, and studying drug-protein dissociation rates will be discussed. Applications that illustrate the use of HPAC with serum binding agents such as human serum albumin, α1-acid glycoprotein, and lipoproteins will be presented. Recent developments will also be examined, such as new methods for immobilizing serum proteins in HPAC columns, the utilization of HPAC as a tool in personalized medicine, and HPAC methods for the high-throughput screening and characterization of drug-protein binding

Pharmaceutical And Biomedical Applications Of Affinity Chromatography: Recent Trends And Developments

Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically-related agent as a stationary phase to selectively retain analytes or to study biological interactions. This review discusses the basic principles behind affinity chromatography and examines recent developments that have occurred in the use of this method for biomedical and pharmaceutical analysis. Techniques based on traditional affinity supports are discussed, but an emphasis is placed on methods in which affinity columns are used as part of HPLC systems or in combination with other analytical methods. General formats for affinity chromatography that are considered include step elution schemes, weak affinity chromatography, affinity extraction and affinity depletion. Specific separation techniques that are examined include lectin affinity chromatography, boronate affinity chromatography, immunoaffinity chromatography, and immobilized metal ion affinity chromatography. Approaches for the study of biological interactions by affinity chromatography are also presented, such as the measurement of equilibrium constants, rate constants, or competition and displacement effects. In addition, related developments in the use of immobilized enzyme reactors, molecularly imprinted polymers, dye ligands and aptamers are briefly considered

Pharmaceutical And Biomedical Applications Of Affinity Chromatography: Recent Trends And Developments

Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically-related agent as a stationary phase to selectively retain analytes or to study biological interactions. This review discusses the basic principles behind affinity chromatography and examines recent developments that have occurred in the use of this method for biomedical and pharmaceutical analysis. Techniques based on traditional affinity supports are discussed, but an emphasis is placed on methods in which affinity columns are used as part of HPLC systems or in combination with other analytical methods. General formats for affinity chromatography that are considered include step elution schemes, weak affinity chromatography, affinity extraction and affinity depletion. Specific separation techniques that are examined include lectin affinity chromatography, boronate affinity chromatography, immunoaffinity chromatography, and immobilized metal ion affinity chromatography. Approaches for the study of biological interactions by affinity chromatography are also presented, such as the measurement of equilibrium constants, rate constants, or competition and displacement effects. In addition, related developments in the use of immobilized enzyme reactors, molecularly imprinted polymers, dye ligands and aptamers are briefly considered

Psicoterapia Integrativa Grupal para Potenciar Habilidades Emocionales. Adolescentes Femeninas en Transición. Aldeas SOS, David, Chiriquí

El presente informe plasma la labor realizada de la práctica profesional, dando a conocer los conocimientos y experiencias adquiridas durante el período de dos meses desarrollando psicoterapias de tipo integrativas en adolescentes de las Aldeas SOS de David, cumpliendo así con el objetivo de la institución de enseñanza superior UDELAS, de promover el aprendizaje significativo y construir competencias que favorezcan el ejercicio profesional. El objetivo particular de esta práctica es llevar la intervención psicoterapéutica integrativa grupal a adolescentes femeninas en riesgo social, que habitan en las Aldeas SOS

Affinity chromatographic studies of drug-protein binding in personalized medicine

In recent years there have been an increasing number of scientific advances dedicated to the transition from traditional medicine to a new era of data-driven personalized medicine. The customization of healthcare holds a great promise for providing faster diagnosis and more effective treatments for a variety of diseases and disorders, such as diabetes. The number of diabetic patients has increased at an alarming rate over the last 20 years, with an estimated 26 million children and adults in the United States suffering from this disease and related complications such as cardiovascular disease, kidney or liver damage, and blindness. Some of these complications have been associated with high levels of blood glucose and a process known as non-enzymatic glycation, which can produce structural and functional modifications of proteins in the human body. Among these proteins, human serum albumin (HSA) is an important transport protein for many drugs, hormones and fatty acids in the circulation. Therefore, a detailed discussion of the process of glycation on HSA is provided in this dissertation. One topic examined in this manuscript is the use of high-performance affinity chromatography (HPAC) and immobilized HSA microcolumns to examine the binding of various drugs to HSA as the glycation level is increased. This work was first performed by studying the binding of a number of sulfonylurea drugs to in vivo glycated HSA. Affinity microcolumns were prepared from only 20 μL of serum from individual patients with diabetes. The clinical samples were also analyzed using mass spectrometry to identify and quantify modifications of HSA that occur due to glycation. In addition, competition studies were used to investigate the effect of in vitro glycation and the presence of long chain fatty acids on the binding of drugs at the major binding sites of HSA. Frontal analysis and zonal elution experiments were also utilized to study the binding of drugs to alpha-acid glycoprotein (AGP), an acute phase protein. A novel on-column (in situ) protein entrapment approach was developed to prepare affinity microcolumns containing AGP. Lastly, the peak decay method and HPAC were used to examine the dissociation rate constants for several species of immunoglobulin G (IgG) from immobilized protein G supports. The approaches developed in this dissertation are not limited to glycated HSA and AGP but could be adapted to other modified proteins and disease states of interest to the scientific community and to the field of personalized medicine