Non-Roundabout design of cancel the intersection signal light on horizontal plane

Traffic congestion is a world problem and an important factor restricting urban development. In order to solve the problem of urban traffic congestion, this paper takes the traffic flow theory and the intersection channel design theory as the research foundation, and conducts in-depth research on the causes of congestion at the intersection and the corresponding solutions, and proposes to cancel the traffic lights at the intersection without any stagnation. This paper proposes a new intersection design scheme, which is like the veins of the flower veins to channel the design intersection, cancel the signal light, and the vehicle can pass through the intersection without stagnation. It proposes a new solution to solve the traffic congestion problem. This new design allows the traffic flow to be spatially separated on the horizontal plane, and due to the cancellation of the signal lights, there is no signal waiting at the intersection, and the vehicle can travel without stopping at the intersection. At the same time, this paper also establishes a plane intersection service capability evaluation system based on simulation and quantitative calculation, which provides an evaluation index and proof basis for the non-stagnation driving channel design of the non-roundabout intersection

Non-Roundabout design of cancel the intersection signal light on horizontal plane

Traffic congestion is a world problem and an important factor restricting urban development. In order to solve the problem of urban traffic congestion, this paper takes the traffic flow theory and the intersection channel design theory as the research foundation, and conducts in-depth research on the causes of congestion at the intersection and the corresponding solutions, and proposes to cancel the traffic lights at the intersection without any stagnation. This paper proposes a new intersection design scheme, which is like the veins of the flower veins to channel the design intersection, cancel the signal light, and the vehicle can pass through the intersection without stagnation. It proposes a new solution to solve the traffic congestion problem. This new design allows the traffic flow to be spatially separated on the horizontal plane, and due to the cancellation of the signal lights, there is no signal waiting at the intersection, and the vehicle can travel without stopping at the intersection. At the same time, this paper also establishes a plane intersection service capability evaluation system based on simulation and quantitative calculation, which provides an evaluation index and proof basis for the non-stagnation driving channel design of the non-roundabout intersection. Document type: Articl

Biofilm formation and antibiotic resistance in staphylococcus isolated from different objects

Staphylococci have a wide range of pathogenic properties, among which stands increased resistance to antibiotics and the ability to form a biofilm. Being divided into coagulase-positive and coagulase-negative, they have different and, at the same time, common biological properties and may be a source of genetic material for each other. The aim of the study was to examine the properties of staphylococci, isolated from various objects (milk, pigs, companion animals, humans), their resistance to antibiotics, the ability to form a biofilm and the presence of genes, responsible for resistance to methicillin and biofilm formation. In this study we used 89 Staphylococci strains. 18 (20.2 %) strains were coagulase-positive, where 2 (2.2 %) strains, isolated from different animals and humans, had a complete set of phenotypic (plasma coagulation, biofilm formation, resistance to oxacillin and benzylpenicillin) and genotypic (mec A, fem B, ica A, ica D) signs of pathogenicity. Coagulase-negative staphylococci, isolated from various animals and humans, had resistance to oxacillin, benzylpenicillin and the ability to form biofilms, and also had the corresponding genes in their structure (mec A, ica AB, ica D) in 3.3 % of cases. Staphylococci, isolated from different animals and humans, were able to form a biofilm and had the appropriate set of genes (ica D, ica AB) in 5.6 % of cases

The development of the solution search method based on the improved bee colony algorithm

Active digitization of people's daily life leads to the use of the decision-making support systems (DMSS). DMSS is actively used in data processing, forecasting the course of various processes, providing informational support for the decision-making process by decision makers. However, a number of problems arise while evaluating monitoring objects, namely: a large number of destabilizing factors affecting the efficiency of the processes of information collection, processing and transmission; high dynamism of changes in the state and composition of heterogeneous monitoring objects during the conduct of hostilities (operations); high dynamism of conducting hostilities (operations); the uncertainty of the initial situation and the noise of the initial data. In this article, a method of finding solutions based on an improved bee colony algorithm was developed. The efficiency of information processing is achieved by learning the architecture of artificial neural networks; taking into account the type of uncertainty of the information to be evaluated; the use of an improved algorithm of the bee colony, the use of an unordered linguistic scale of measurements with adjustment coefficients for the degree of awareness and the degree of noise of the initial data. An approbation of the use of the proposed method was carried out on the example of assessing the state of the operational grouping of troops (forces). The method is proposed to be used in the development of software for automated systems of control of troops and weapons, namely, in the modernization of existing and development of new automated systems of control of troops and weapons. The evaluation of the effectiveness of the proposed method showed an increase in the efficiency of the evaluation at the level of 21–28 % in terms of the efficiency of information processin

Визначення раціональних параметрів ємнісного накопичувача енергії для рухомого складу метрополітену

An approach was proposed for determining rational parameters of the on-board capacitive energy storage for the underground railway rolling stock with recuperation systems. The essence of the approach is to determine the maximum power and energy intensity of the on-board CESS based on the analysis of the payback period of the storage systems. The main distinctive features of this approach include taking into account a number of operating conditions and technical features of the underground railway rolling stock.Using this approach, rational parameters of the on-board capacitive energy storage system for standard operating conditions of the experimental rolling stock with the recovery systems were determined at the Sviatoshyno ‒ Brovary line of the Kyiv Metropoliten. Based on the analysis of diagrams of the payback period of the chosen storage systems, it was established that for the given conditions of operation of the underground railway rolling stock, it is rational to introduce a capacitive storage with a maximum power of 1,000 kW and a working energy intensity of 3 kWh. It was calculated that for the given operating conditions, introduction of a storage system with rational parameters will save 16.1 % of the quantity of electric energy consumed for traction of the underground railway rolling stock.Предложен новый научный подход для определения рациональных параметров емкостного накопителя энергии (ЕНЭ) для подвижного состава метрополитена, суть которого заключается в определении максимальной мощности и энергоемкости бортового ЕНЭ по анализу термина окупаемости систем накопления. Установлено, что для заданных условий эксплуатации подвижного состава метрополитена рациональным является внедрение накопителя, максимальная мощность которого составляет 1000 кВт, а рабочая энергоемкость – 3 кВт·часЗапропоновано підхід для визначення раціональних параметрів ємнісного накопичувача енергії (ЄНЕ) для рухомого складу метрополітену, суть якого полягає у визначенні максимальної потужності та енергоємності бортового ЄНЕ за аналізом терміну окупності систем накопичення. Встановлено, що для заданих умов експлуатації рухомого складу метрополітену раціональним є впровадження ЄНЕ, максимальна потужність якого складає 1000 кВт, а робоча енергоємність – 3 кВт·го

Визначення раціональних параметрів ємнісного накопичувача енергії для рухомого складу метрополітену

An approach was proposed for determining rational parameters of the on-board capacitive energy storage for the underground railway rolling stock with recuperation systems. The essence of the approach is to determine the maximum power and energy intensity of the on-board CESS based on the analysis of the payback period of the storage systems. The main distinctive features of this approach include taking into account a number of operating conditions and technical features of the underground railway rolling stock.Using this approach, rational parameters of the on-board capacitive energy storage system for standard operating conditions of the experimental rolling stock with the recovery systems were determined at the Sviatoshyno ‒ Brovary line of the Kyiv Metropoliten. Based on the analysis of diagrams of the payback period of the chosen storage systems, it was established that for the given conditions of operation of the underground railway rolling stock, it is rational to introduce a capacitive storage with a maximum power of 1,000 kW and a working energy intensity of 3 kWh. It was calculated that for the given operating conditions, introduction of a storage system with rational parameters will save 16.1 % of the quantity of electric energy consumed for traction of the underground railway rolling stock.Предложен новый научный подход для определения рациональных параметров емкостного накопителя энергии (ЕНЭ) для подвижного состава метрополитена, суть которого заключается в определении максимальной мощности и энергоемкости бортового ЕНЭ по анализу термина окупаемости систем накопления. Установлено, что для заданных условий эксплуатации подвижного состава метрополитена рациональным является внедрение накопителя, максимальная мощность которого составляет 1000 кВт, а рабочая энергоемкость – 3 кВт·часЗапропоновано підхід для визначення раціональних параметрів ємнісного накопичувача енергії (ЄНЕ) для рухомого складу метрополітену, суть якого полягає у визначенні максимальної потужності та енергоємності бортового ЄНЕ за аналізом терміну окупності систем накопичення. Встановлено, що для заданих умов експлуатації рухомого складу метрополітену раціональним є впровадження ЄНЕ, максимальна потужність якого складає 1000 кВт, а робоча енергоємність – 3 кВт·го

Structural and functional investigation of flavin binding center of the NqrC subunit of sodium-translocating NADH:Quinone oxidoreductase from Vibrio harveyi

Na+-translocating NADH:quinone oxidoreductase (NQR) is a redox-driven sodium pump operating in the respiratory chain of various bacteria, including pathogenic species. The enzyme has a unique set of redox active prosthetic groups, which includes two covalently bound flavin mononucleotide (FMN) residues attached to threonine residues in subunits NqrB and NqrC. The reason of FMN covalent bonding in the subunits has not been established yet. In the current work, binding of free FMN to the apo-form of NqrC from Vibrio harveyi was studied showing very low affinity of NqrC to FMN in the absence of its covalent bonding. To study structural aspects of flavin binding in NqrC, its holo-form was crystallized and its 3D structure was solved at 1.56 Å resolution. It was found that the isoalloxazine moiety of the FMN residue is buried in a hydrophobic cavity and that its pyrimidine ring is squeezed between hydrophobic amino acid residues while its benzene ring is extended from the protein surroundings. This structure of the flavin-binding pocket appears to provide flexibility of the benzene ring, which can help the FMN residue to take the bended conformation and thus to stabilize the one-electron reduced form of the prosthetic group. These properties may also lead to relatively weak noncovalent binding of the flavin. This fact along with periplasmic location of the FMN-binding domains in the vast majority of NqrC-like proteins may explain the necessity of the covalent bonding of this prosthetic group to prevent its loss to the external medium

Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody

Highly selective monoclonal antibodies recognizing the extracellular 3D epitope of G protein-coupled receptors represent valuable tools for elucidating receptor function and localization in the cell and show promise for a range of therapeutic applications. Here we present the structure of a complex between the human serotonin 2B receptor, captured in an active-like state, and an antibody Fab fragment, bound to the extracellular side of the receptor. The structure uncovers the mechanisms of receptor activation and of extracellular receptor recognition by antibodies

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser.

G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin-arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a ∼20° rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. This structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology

Фундаментальні основи забезпечення стійкого розвитку при переході до інформаційного суспільства

Метою дослдіження є теоретичне обґрунтування та дослідження фундаментальних основ відтворення стійкого стану відкритих стаціонарних систем. При цитуванні документа, використовуйте посилання http://essuir.sumdu.edu.ua/handle/123456789/1347