Postexposure Treatment of Marburg Virus Infection

Rhesus monkeys are protected from disease when a recombinant vesicular stomatitis virus–based vaccine is administered 20–30 min after infection with Marburg virus. We protected 5/6 monkeys when this vaccine was given 24 h after challenge; 2/6 animals were protected when the vaccine was administered 48 h postinfection

A procedural development for the analysis of ^56/54Fe and ^57/54Fe isotope ratios with new generation IsoProbe MC-ICP-MS

We have developed a procedure for iron isotope analysis using a hexapole collision cell MC-ICP-MS which is capable of Fe isotope ratio analysis using two different extraction modes. Matrix effects were minimised and the signal-to-background ratio was maximised using high-concentration samples (~ 5μg Fe) and introducing 1.8 mL/min<sup>-1</sup> Ar and 2 mL/min H<sub>2</sub> into the collision cell to decrease polyatomic interferences. The use of large intensity on the faraday cups considerably decreases the internal error of the ratios and ultimately, improves the external precision of a run. Standard bracketing correction for mass bias was possible when using hard extraction. Mass bias in soft extraction mode seems to show temporal instability that makes the standard bracketing inappropriate. The hexapole rf amplitude was decreased to 50 % to further decrease polyatomic interferences and promote the transmission of iron range masses. We routinely measure Fe isotopes with a precision of ± 0.05 ‰ and ± 0.12 ‰ (2σ) for δ<sup>56</sup>Fe and δ<sup>57</sup>Fe respectively

The rise of the citizen author: Writing within social media

The concept of the citizen author is defined and explored within the publishing industry. In order to understand what positions the citizen author currently, and potentially could, hold it begins with a historical view of their rise, including concepts of their eighteenth century antecedents. But the focus of this research is on their growth alongside that of social media platforms. This allows for drawing out relationships between genre fiction, publishers, and the citizen author, which provides a more full understanding of the power dynamics involved when publishers, social media, and the citizen authors mix in the current industry climate.N/

Effects of radiation therapy on tissue and serum concentrations of tumour associated trypsin inhibitor and their prognostic significance in rectal cancer patients

<p>Abstract</p> <p>Background</p> <p>We have previously demonstrated that elevated concentrations of tumour-associated trypsin inhibitor (TATI) in both tumour tissue (t-TATI) and in serum (s-TATI) are associated with a poor prognosis in colorectal cancer patients. It was also found that s-TATI concentrations were lower in patients with rectal cancer compared to patients with colon cancer. In this study, we investigated the effects of neoadjuvant radiotherapy (RT) on concentrations of t-TATI and s-TATI in patients with rectal cancer.</p> <p>Methods</p> <p>TATI was analysed in serum, normal mucosa and tumour tissue collected at various time points in 53 rectal cancer patients enrolled in a case-control study where 12 patients received surgery alone, 20 patients 5 × 5 Gy (short-term) preoperative RT and 21 patients 25 × 2 Gy (long-term) preoperative RT. T-TATI was analysed by immunohistochemistry and s-TATI was determined by an immunofluorometric assay. Mann-Whitney U test and Wilcoxon Z (Z) test were used to assess t-TATI and s-TATI concentrations in relation to RT. Spearman's correlation (R) test was used to explore the associations between t-TATI, s-TATI and clinicopathological parameters. Overall survival (OS) according to high and low t-TATI and s-TATI concentrations was estimated by classification and regression tree analysis, Kaplan-Meier analysis and the log rank test.</p> <p>Results</p> <p>RT did not affect concentrations of t-TATI or s-TATI. In patients receiving short-term but not long-term RT, s-TATI concentrations were significantly higher 4 weeks post surgery than in serum drawn prior to surgery (Z = -3.366, P < 0.001). T-TATI expression correlated with male gender (R = 0.406, P = 0.008). High t-TATI expression in surgical specimens was associated with a significantly shorter OS (P = 0.045). S-TATI concentrations in serum drawn at all time points were associated with an impaired OS (P = 0.035 before RT, P = 0.001 prior to surgery, P = 0.043 post surgery). At all time points, s-TATI correlated with higher age (P < 0.001-0.021) and with increased s-creatinine concentrations assessed prior to surgery (P = 0.041).</p> <p>Conclusions</p> <p>The results presented here further validate the utility of t-TATI and s-TATI as prognostic biomarkers in patients with rectal cancer, independent of neoadjuvant RT.</p

Results from E-Mevva and Z-Mevva: Development of new sources of intense high charge state heavy-ion beams

The authors are exploring a new approach for heavy ion beam injection (e.g., into the Relativistic Heavy Ion Collider (RHIC) at BNL), as well as new sources of intense high charge state ions to be mounted on a relatively low voltage platform for high energy ion implantation. While conventional Metal Vapor Vacuum Arc (Mevva) ion sources can produce up to hundreds of milliamps or more of several-times-ionized metal ions (e.g., U3+), the recent results from Batalin et al. indicate that the addition of an energetic electron beam (E-Mevva) may lead to considerably higher charge states. An alternative way to produce the electron beam is Z-Mevva, in which a z-discharge plasma is used to enhance multiple ionization. As the vacuum arc plasma plume expands into a magnetized drift region, a z-discharge is triggered in the drifting metal plasma. The ions are then extracted and analyzed using a time-of-flight system. The authors report initial results using these schemes with applied discharge and electron beam voltages from 1 to 2 kV

The evolution of low-metallicity asymptotic giant branch stars and the formation of carbon-enhanced metal-poor stars

We investigate the behaviour of asymptotic giant branch (AGB) stars between metallicities Z = 10-4 and Z = 10-8 . We determine which stars undergo an episode of flash-driven mixing, where protons are ingested into the intershell convection zone, as they enter the thermally pulsing AGB phase and which undergo third dredge-up. We find that flash-driven mixing does not occur above a metallicity of Z = 10-5 for any mass of star and that stars above 2 M do not experience this phenomenon at any metallicity. We find carbon ingestion (CI), the mixing of carbon into the tail of hydrogen burning region, occurs in the mass range 2 M to around 4 M . We suggest that CI may be a weak version of the flash-driven mechanism. We also investigate the effects of convective overshooting on the behaviour of these objects. Our models struggle to explain the frequency of CEMP stars that have both significant carbon and nitrogen enhancement. Carbon can be enhanced through flash-driven mixing, CI or just third dredge up. Nitrogen can be enhanced through hot bottom burning and the occurrence of hot dredge-up also converts carbon into nitrogen. The C/N ratio may be a good indicator of the mass of the primary AGB stars.Comment: 15 pages, 13 figures, 1 table, accepted by MNRA

The puzzling dredge-up pattern in NGC 1978

Low-mass stars are element factories that efficiently release their products in the final stages of their evolution by means of stellar winds. Since they are large in number, they contribute significantly to the cosmic matter cycle. To assess this contribution quantitatively, it is crucial to obtain a detailed picture of the stellar interior, particularly with regard to nucleosynthesis and mixing mechanisms. We seek to benchmark stellar evolutionary models of low-mass stars. In particular, we measure the surface abundance of ^{12}C in thermally pulsing AGB stars with well-known mass and metallicity, which can be used to infer information about the onset and efficiency of the third dredge-up. We recorded high-resolution near-infrared spectra of AGB stars in the LMC cluster NGC 1978. The sample comprised both oxygen-rich and carbon-rich stars, and is well-constrained in terms of the stellar mass, metallicity, and age. We derived the C/O and ^{12}C/^{13}C ratio from the target spectra by a comparison to synthetic spectra. Then, we compared the outcomes of stellar evolutionary models with our measurements. The M stars in NGC 1978 show values of C/O and ^{12}C/^{13}C that can best be explained with moderate extra-mixing on the RGB coupled to a moderate oxygen enhancement in the chemical composition. These oxygen-rich stars do not seem to have undergone third dredge-up episodes (yet). The C stars show carbon-to-oxygen and carbon isotopic ratios consistent with the occurrence of the third dredge-up. We did not find S stars in this cluster. None of the theoretical schemes that we considered was able to reproduce the observations appropriately. Instead, we discuss some non-standard scenarios to explain the puzzling abundance pattern in NGC 1978.Comment: 16 pages, 9 figures, 4 tables, accepted for publication in A&A, language revise

Response to the COVID-19 Pandemic Among Posthospital Brain Injury Rehabilitation Providers

Rehabilitation after significant acquired brain injury (ABI) to address complex independent activities of daily living and return to family and community life is offered primarily after initial hospitalization in outpatient day treatment, group home, skilled nursing, and residential settings and in the home and community of the person served. The coronavirus 2019 pandemic threatened access to care and the health and safety of staff, persons served, and families in these settings. This article describes steps taken to contain this threat by 7 leading posthospital ABI rehabilitation organizations. Outpatient and day treatment facilities were temporarily suspended. In other settings, procedures for isolation, transportation, cleaning, exposure control, infection control, and use of personal protective equipment (PPE) were reinforced with staff. Visitation and community activities were restricted. Staff and others required to enter facilities were screened with symptom checklists and temperature checks. Individuals showing symptoms of infection were quarantined and tested, as possible. New admissions were carefully screened for infection and often initially quarantined. Telehealth played a major role in reducing direct interpersonal contact while continuing to provide services both to outpatients and within facilities. Salary, benefits, training, and managerial support were enhanced for staff. Despite early outbreaks, these procedures were generally effective, with preliminary initial infections rates of only 1.1% for persons served and 2.1% for staff. Reductions in admissions, services, and unanticipated expenses (eg, PPE, more frequent and thorough cleaning) had a major negative financial effect. Providers continue to be challenged to adapt rehabilitative approaches and to reopen services.Supported by the Foundation for the Advancement of Brain Rehabilitation (FABR)

Polymorphisms in Plasmodium falciparum chloroquine resistance transporter and multidrug resistance 1 genes: parasite risk factors that affect treatment outcomes for P. falciparum malaria after artemether-lumefantrine and artesunate-amodiaquine.

Adequate clinical and parasitologic cure by artemisinin combination therapies relies on the artemisinin component and the partner drug. Polymorphisms in the Plasmodium falciparum chloroquine resistance transporter (pfcrt) and P. falciparum multidrug resistance 1 (pfmdr1) genes are associated with decreased sensitivity to amodiaquine and lumefantrine, but effects of these polymorphisms on therapeutic responses to artesunate-amodiaquine (ASAQ) and artemether-lumefantrine (AL) have not been clearly defined. Individual patient data from 31 clinical trials were harmonized and pooled by using standardized methods from the WorldWide Antimalarial Resistance Network. Data for more than 7,000 patients were analyzed to assess relationships between parasite polymorphisms in pfcrt and pfmdr1 and clinically relevant outcomes after treatment with AL or ASAQ. Presence of the pfmdr1 gene N86 (adjusted hazards ratio = 4.74, 95% confidence interval = 2.29 - 9.78, P < 0.001) and increased pfmdr1 copy number (adjusted hazards ratio = 6.52, 95% confidence interval = 2.36-17.97, P < 0.001 : were significant independent risk factors for recrudescence in patients treated with AL. AL and ASAQ exerted opposing selective effects on single-nucleotide polymorphisms in pfcrt and pfmdr1. Monitoring selection and responding to emerging signs of drug resistance are critical tools for preserving efficacy of artemisinin combination therapies; determination of the prevalence of at least pfcrt K76T and pfmdr1 N86Y should now be routine

On the nature of the galactic early-B hypergiants

Despite their importance to a number of astrophysical fields, the lifecycles of very massive stars are still poorly defined. In order to address this shortcoming, we present a detailed quantitative study of the physical properties of four early-B hypergiants (BHGs); Cyg OB2 #12, zeta Sco, HD190603 and BP Cru. These are combined with an analysis of their long-term spectroscopic and photometric behaviour in order to determine their evolutionary status. The long-term datasets revealed that they are remarkably stable over long periods (>40yr), with the possible exception of zeta Sco prior to the 20th century, in contrast to the typical excursions that characterise luminous blue variables (LBVs). Zeta Sco, HD190603 and BP Cru possess physical properties intermediate between B supergiants and LBVs; we therefore suggest that BHGs are the immediate descendants and progenitors (respectively) of such stars (for initial masses in the range ~30-60Msun). In contrast, while the wind properties of Cyg OB2 #12 are consistent with this hypothesis, the combination of extreme luminosity and spectroscopic mass (~110Msun) and comparatively low temperature means it cannot be accommodated in such a scheme. Likewise, despite its co-location with several LBVs above the Humphreys-Davidson (HD) limit, the lack of long term variability and its unevolved chemistry apparently excludes such an identification. Since such massive stars are not expected to evolve to such cool temperatures, the properties of Cyg OB2 #12 are difficult to understand under current evolutionary paradigms. [ABRIDGED]Comment: 36 pages, 19 figures (of which 17 pages are online supplemental material). Accepted for publication in Astronomy and Astrophysic