Characterization of laurel fruit oil from Madeira Island, Portugal

The fixed oil extracted from Laurus spp. fruit from Madeira Island, Portugal, is used in local traditional medicine for a wide variety of health complaints. Physical properties, density and refractive index, as well as the TAG FA composition, sterols, and waxes were determined. The oil was found to have an un usually high content of volatiles (ca. 10%), with trans-ocimene and germacrene D predominating. Oleic (30%) and linoleic (20%) acids were the main unsaturated FA, whereas lauric (18%) and palmitic (up to 22.5%) acids were the main saturated FA in the neutral lipid fraction. The oil had a sterol content on the same order as olive oil, with β-sitosterol (84%) predominating. Two sesquiterpene lactones, dehydrocostuslactone and costunolide, accounted for 5% of the overall composition. Madeira laurel oil is not currently used as an edible oil because of its very strong flavor. Its claimed medicinal properties have not yet been vali dated, and this is the first report on the characterization of the commercial product.info:eu-repo/semantics/publishedVersio

Detection methods for aflatoxin M1 in dairy products

Mycotoxins are toxic compounds produced mainly by fungi of the genera Aspergillus, Fusarium and Penicillium. In the food chain, the original mycotoxin may be transformed in other toxic compounds, reaching the consumer. A good example is the occurrence of aflatoxin M1 (AFM1) in dairy products, which is due to the presence of aflatoxin B1 (AFB1) in the animal feed. Thus, milk-based foods, such as cheese and yogurts, may be contaminated with this toxin, which, although less toxic than AFB1, also exhibits hepatotoxic and carcinogenic effects and is relatively stable during pasteurization, storage and processing. For this reason, the establishment of allowed maximum limits in dairy products and the development of methodologies for its detection and quantification are of extreme importance. There are several methods for the detection of AFM1 in dairy products. Usually, the analytical procedures go through the following stages: sampling, extraction, clean-up, determination and quantification. For the extraction stage, the use of organic solvents (as acetonitrile and methanol) is still the most common, but recent advances include the use of the Quick, Easy, Cheap, Effective, Rugged, and Safe method (QuEChERS) and proteolytic enzymes, which have been demonstrated to be good alternatives. For the clean-up stage, the high selectivity of immunoaffinity columns is still a good option, but alternative and cheaper techniques are becoming more competitive. Regarding quantification of the toxin, screening strategies include the use of the enzyme-linked immunosorbent assay (ELISA) to select presumptive positive samples from a wider range of samples, and more reliable methods—high performance liquid chromatography with fluorescence detection or mass spectroscopy—for the separation, identification and quantification of the toxin.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. PR is grateful to FCT and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019). We would like to thank the Foundation for Science and Technology (FCT) for the Ph.D. scholarship given to Andreia Vaz (SFRH/BD/129775/2017).info:eu-repo/semantics/publishedVersio

Exploratory study on maturation and competition level in young karate practitioners

Emparelhar crianças propicia sucesso e reduz lesões no desporto. Apesar das diferenças de maturação entre crianças, a idade cronológica mantém-se o principal critério para equalizar os níveis de competição, mas com uma eficiência limitada. No entanto, no karaté, o peso também é considerado. Fomos verificar se existe associação entre a idade cronológica e os indicadores maturacionais e, encontrar modelos simples que permitam colocar em reflexão novas formas de avaliação da maturação. Foram recolhidos dados em 54 jovens praticantes de karaté (idade: 12,67 ± 2,49 anos; experiência: 4,99 ± 2,44 anos; altura: 150,45 ± 15,25cm; peso: 43,09 ± 14,17 kg, 20 raparigas). A idade correlacionou-se significativamente com auto-observação da maturação sexual (rho = 0,838; p < 0,01), e foi possível encontrar modelos de regressão simples que têm uma explicação moderada na maturação, apenas utilizando variáveis de medição simples, tal como a altura, o peso e o perímetro do braço. Estes resultados devem levar as entidades competentes a refletir acerca do modo de emparelhamento dos atletas nas competições de uma forma justa em termos de maturação.Paring children improve the chance for success and reduce injury in sport. Despite the maturity differences between children, chronological age remains the main criterion to equalize competition levels, but with limited efficiency. However, in Karate, weight is also considered. We went to investigate the association between chronological age and maturational indicators and find simple models to put on reflection new ways to assess the maturity. Data were collected in 54 young Karate practitioners (age: 12.67 ± 2.49 years, experience: 4.99 ± 2.44 years, height: 150.45 ± 15.25 cm, weight: 43.09 ± 14, 17 kg, 20 girls). Age correlated significantly with self-observation of sexual maturation (rho = 0.838; p < 0.01), and it was possible to find simple regression models that have a moderate explanation in maturation, using only simple measurable variables such as the height, weight and arm circumference. These results should lead the competent entities to reflect on the way of pairing of the athletes in the competitions, in a fair way in terms of maturation.info:eu-repo/semantics/publishedVersio

Developmental arrest in Drosophila melanogaster caused by mitochondrial DNA replication defects cannot be rescued by the alternative oxidase

The xenotopic expression of the alternative oxidase AOX from the tunicate Ciona intestinalis in diverse models of human disease partially alleviates the phenotypic effects of mitochondrial respiratory chain defects. AOX is a non-proton pumping, mitochondrial inner membrane-bound, single-subunit enzyme that can bypass electron transport through the cytochrome segment, providing an additional site for ubiquinone reoxidation and oxygen reduction upon respiratory chain overload. We set out to investigate whether AOX expression in Drosophila could counteract the effects of mitochondrial DNA (mtDNA) replication defects caused by disturbances in the mtDNA helicase or DNA polymerase gamma. We observed that the developmental arrest imposed by either the expression of mutant forms of these enzymes or their knockdown was not rescued by AOX. Considering also the inability of AOX to ameliorate the phenotype of tko(25t), a fly mutant with mitochondrial translation deficiency, we infer that this alternative enzyme may not be applicable to cases of mitochondrial gene expression defects. Finding the limitations of AOX applicability will help establish the parameters for the future putative use of this enzyme in gene therapies for human mitochondrial diseases.Peer reviewe

Characterization of triacylglycerols in Madeira laurel oil by HPLC-atmospheric pressure chemical ionization-MS

Madeira laurel oil was fractionated by liquid ex traction combined with TLC, and TAGs were analyzed by HPLC coupled with atmospheric pressure chemical ionization-MS (APCI-MS). Eluted molecular species compositions of the eluted TAG in the complex natural mixture were determined by GC identification of FAME and by LC-atmospheric pressure chemi cal ionization (APCI)-MS analysis of the lipid. The APCI-MS spectra of most TAG exhibited [M + H]+ and [M − RCOO]+ ions, which defined the M.W. and the molecular association of fatty acyl residues, respectively. Despite the relatively high de gree of saturation, with a saturated/unsaturated ratio of 0.70, no totally saturated TAG nor mixed asymmetric TAG with two sat urated FA (SSM or SSU, where S is saturated, M is monounsatu rated, and U is unsaturated) were found. This type of molecular structure provides a possible explanation for the relatively low m.p. (12–15°C) and also the high oxidative resistance observed.info:eu-repo/semantics/publishedVersio

Tarefas em geometria: da sala de aula para a formação de professores, descrição de um projeto

Neste poster, apresentamos um projeto que pretende abordar de forma conglomerada conhecimentos, raciocínios, argumentação e representações de alunos e professores dos primeiros anos. Perspetiva-se como uma das formas de contribuir para uma melhoria dos conhecimentos geométricos de alunos e professores bem como para uma possível mudança de foco na prática e na formação geométrica de professores

Effects of foliar and soil boron fertilization on yield, leaf physiological traits and fruit attributes in rainfed almond orchards

Boron (B) fertilizers are recognized as essential for ensuring yield and fruit quality. However, the importance of soil and foliar B fertilization in almond orchards under rainfed conditions is presently unclear. To address this literature gap, in the present study, the impact of soil and foliar application of B on leaf gas exchange, leaf photosynthetic pigments, yield, and fruit quality in almonds was investigated across three consecutive growing seasons. Boron fertilizer was applied to the soil at four rates (0, 1, 2, and 3 kg ha−1) in the presence or absence of foliar application (0.36 g L−1) of the same micronutrient. Borax pentahydrate was used as the B source. When compared to foliar B fertilization, the soil application of B positively affected the gas exchange parameters, mainly the net CO2 assimilation rate, stomatal conductance, and transpiration rate (percentage of gain between 15% and 80%), but did not influence the chlorophyll content. The almond yield and fruit characteristics were also enhanced (about 25–72%) in response to lower soil B fertilization rates. In the 3 kg ha−1 dose, B had an adverse effect on the yield and resulted in lighter fruits. On the other hand, foliar B fertilization did not benefit any of the evaluated parameters. Overall, these results suggest that, under the studied conditions, almond orchards do not respond to higher B rates in soil or foliar B fertilization.This research work was funded by the project “Estratégias integradas para o aumento da produtividade da amendoeira em Trás-os-Montes”, no. 54611, funded by the EAFRD (European Agricultural Fund for Rural Development), and by the Portuguese State through the “Medida 4.1. Cooperação para a Inovação do programa PRODER-Programa de Desenvolvimento Rural”. The authors also acknowledge to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UIDB/00690/2020) and CITAB (UIDB/04033/2020).info:eu-repo/semantics/publishedVersio

Enrichment of Grapes with Zinc-Efficiency of Foliar Fertilization with ZnSO4 and ZnO and Implications on Winemaking

Grapes and wine are widely consumed in the world, yet their mineral content can be influenced by many factors such as the mineral composition of soils, viticulture practices and environmental conditions. In this context, considering the importance of Zn in the human physiology, the enrichment of Moscatel and Castelão grapes (white and red variety, respectively) with this nutrient prompted this study; further assessment of tissue deposition and some implications for wine production. Using two foliar fertilizers (ZnO or ZnSO4, at 150, 450 and 900 g ha1), decreases in net photosynthesis and stomatal conductance occurred in both varieties, suggesting that the physiological threshold of Zn toxicity was reached without visible symptoms. Following foliar spraying with both fertilizers, the content of Zn in leaves of the Castelão and Moscatel varieties showed higher values in all treatments relative to the control. Moreover, in grapes this tendency occurred only in Castelão. Concerning Cu, Fe, Ca, K, S and P, some significant differences also happened in leaves and grapes among treatments. At harvest, the indexes of Zn enrichment in grapes increased between 2.14- and 8.38-fold and between 1.02- and 1.44-fold in Castelão and Moscatel varieties, respectively. Zinc in the dried skin of Castelão only increased with ZnO and ZnSO4 sprayed at 900 g ha1 (ca. 2.71- and 1.5-fold relative to the control, respectively), but in Moscatel a clear accumulation trend could not be found. The dry weight of grapes ranged (in %) between 16 and 23 (but did not vary significantly among treatments of each variety or in each treatment between varieties), and total soluble solids (e.g., mainly soluble sugars and proteins) and color parameters showed some significant variations. Through winemaking, the contents of Zn increased in both varieties (1.34- and 3.57-fold, in Castelão and Moscatel, respectively) and in all treatments, although non-significantly in Castelão. It is concluded that, to increase the contents of Zn in grapes without reaching the threshold of toxicity ZnO or ZnSO4 can be used for foliar spraying of Castelão and Moscatel varieties until 900 g ha1 and that winemaking augments the level of this nutrientinfo:eu-repo/semantics/publishedVersio

Zinc Biofortification in Vitis vinifera: Implications for Quality and Wine Production

Nowadays, there is a growing concern about micronutrient deficits in food products, with agronomic biofortification being considered a mitigation strategy. In this context, as Zn is essential for growth and maintenance of human health, a workflow for the biofortification of grapes from the Vitis vinifera variety Fernão Pires, which contains this nutrient, was carried out considering the soil properties of the vineyard. Additionally, Zn accumulation in the tissues of the grapes and the implications for some quality parameters and on winemaking were assessed. Vines were sprayed three times with ZnO and ZnSO4 at concentrations of 150, 450, and 900 g ha1 during the production cycle. Physiological data were obtained through chlorophyll a fluorescence data, to access the potential symptoms of toxicity. At harvest, treated grapes revealed significant increases of Zn concentration relative to the control, being more pronounced for ZnO and ZnSO4 in the skin and seeds, respectively. After winemaking, an increase was also found regarding the control (i.e., 1.59-fold with ZnSO4-450 g ha1). The contents of the sugars and fatty acids, as well as the colorimetric analyses, were also assessed, but significant variations were not found among treatments. In general, Zn biofortification increased with ZnO and ZnSO4, without significantly affecting the physicochemical characteristics of grapesinfo:eu-repo/semantics/publishedVersio