IMPLEMENTASI PENATAAN PEDAGANG KAKI LIMA DI KABUPATEN SEMARANG BERDASARKAN PERATURAN DAERAH NOMOR 3 TAHUN 2014 TENTANG PENATAAN DAN PEMBERDAYAAN PEDAGANG KAKI LIMA KABUPATEN SEMARANG

Penelitian ini bertujuan untuk mendeskripsikan Implementasi Penataan Pedagang Kaki Lima Di Kabupaten Semarang Berdasarkan Peraturan Daerah Nomor 3 Tahun 2014 Tentang Penataan Dan Pemberdayaan Pedagang Kaki Lima Kabupaten Semarang, dalam upaya pelaksanaan penataan dan ketertiban para pedagang (PKL) yang berada di kawasan Alun-Alun Lama Ungaran dan Pasar Bandarjo Ungaran. Jenis penelitian yang dipergunakan adalah yuridis sosiologis. Dengan spesifikasi penelitian yaitu penelitian deskriptif analitis. Menggunakan metode penentuan sampel yaitu purposive sampling, sedangkan dalam metode pengumpulan data yaitu data primer, data sekunder dan data tersier. Data primer yang diperoleh langsung dari penelitian di lapangan. Adapun terdapat metode analisis data yaitu dengan menggunakan analisis kualitatif. Berdasarkan hasil penelitian yang dilakukan, dapat disimpulkan bahwa mengenai Implementasi Penataan Pedagang Kaki Lima Di Kabupaten Semarang Berdasarkan Peraturan Daerah Nomor 3 Tahun 2014 pada lingkup Kabupaten Semarang terutama di Alun-Alun Lama Ungaran dan Pasar Bandarjo Ungaran, sudah cukup terlaksana dengan baik. Pedagang (PKL) sudah banyak mengetahui dan memahami adanya Perda Kabupaten Semarang Nomor 3 Tahun 2014 Tentang Penataan Dan Pemberdayaan Pedagang Kaki Lima Kabupaten Semarang. Kendala dalam melaksanakan penelitian yaitu saat melakukan penelitian di kantor Satpol PP, peneliti mendapatkan informasi melalui pesan whatsapp. Dan kendala saat di lapangan pada pedagang (PKL) yaitu pedagang yang masih belum bisa teratur atau kategori pedagang baru, pelanggaran lokasi yaitu menempatkan dagangannya di trotoar dan di sepanjang jalan atau kawasan tertib, untuk PKL yang berdagang di trotoar belum disediakan tempat dari pemerintah. Aturan ini berlaku pada siang hari, kalau malam hari diperbolehkan

The Effects of Eccentric and Plyometric Training Programs and Their Combination on Stability and the Functional Performance in the Post-ACL-Surgical Rehabilitation Period of Elite Female Athletes

BackgroundThe standard method to treat physically active patients with anterior cruciate ligament (ACL) rupture is ligament reconstruction surgery. The rehabilitation training program is very important to improve functional performance in recreational athletes following ACL reconstruction.ObjectivesThe aims of this study were to compare the effects of three different training programs, eccentric training (ECC), plyometric training (PLYO), or combined eccentric and plyometric training (COMB), on dynamic balance (Y-BAL), the Lysholm Knee Scale (LKS), the return to sport index (RSI), and the leg symmetry index (LSI) for the single leg hop test for distance in elite female athletes after ACL surgery.Materials and MethodsFourteen weeks after rehabilitation from surgery, 40 elite female athletes (20.3 ± 3.2 years), who had undergone an ACL reconstruction, participated in a short-term (6 weeks; two times a week) training study. All participants received the same rehabilitation protocol prior to the training study. Athletes were randomly assigned to three experimental groups, ECC (n = 10), PLYO (n = 10), and COMB (n = 10), and to a control group (CON: n = 10). Testing was conducted before and after the 6-week training programs and included the Y-BAL, LKS, and RSI. LSI was assessed after the 6-week training programs only.ResultsAdherence rate was 100% across all groups and no training or test-related injuries were reported. No significant between-group baseline differences (pre-6-week training) were observed for any of the parameters. Significant group-by-time interactions were found for Y-BAL (p < 0.001, ES = 1.73), LKS (p < 0.001, ES = 0.76), and RSI (p < 0.001, ES = 1.39). Contrast analysis demonstrated that COMB yielded significantly greater improvements in Y-BAL, LKS, and RSI (all p < 0.001), in addition to significantly better performances in LSI (all p < 0.001), than CON, PLYO, and ECC, respectively.ConclusionIn conclusion, combined (eccentric/plyometric) training seems to represent the most effective training method as it exerts positive effects on both stability and functional performance in the post-ACL-surgical rehabilitation period of elite female athletes

Exploring the genetic diversity and population structure of wheat landrace population conserved at ICARDA genebank

Landraces are considered a valuable source of potential genetic diversity that could be used in the selection process in any plant breeding program. Here, we assembled a population of 600 bread wheat landraces collected from eight different countries, conserved at the ICARDA's genebank, and evaluated the genetic diversity and the population structure of the landraces using single nucleotide polymorphism (SNP) markers. A total of 11,830 high-quality SNPs distributed across the genomes A (40.5%), B (45.9%), and D (13.6%) were used for the final analysis. The population structure analysis was evaluated using the model-based method (STRUCTURE) and distance-based methods [discriminant analysis of principal components (DAPC) and principal component analysis (PCA)]. The STRUCTURE method grouped the landraces into two major clusters, with the landraces from Syria and Turkey forming two clusters with high proportions of admixture, whereas the DAPC and PCA analysis grouped the population into three subpopulations mostly according to the geographical information of the landraces, i.e., Syria, Iran, and Turkey with admixture. The analysis of molecular variance revealed that the majority of the variation was due to genetic differences within the populations as compared with between subpopulations, and it was the same for both the cluster-based and distance-based methods. Genetic distance analysis was also studied to estimate the differences between the landraces from different countries, and it was observed that the maximum genetic distance (0.389) was between the landraces from Spain and Palestine, whereas the minimum genetic distance (0.013) was observed between the landraces from Syria and Turkey. It was concluded from the study that the model-based methods (DAPC and PCA) could dissect the population structure more precisely when compared with the STRUCTURE method. The population structure and genetic diversity analysis of the bread wheat landraces presented here highlight the complex genetic architecture of the landraces native to the Fertile Crescent region. The results of this study provide useful information for the genetic improvement of hexaploid wheat and facilitate the use of landraces in wheat breeding programs

Contribution to the study of the mechanisms of anti-oxidant and anti-inflammatory glioprotection on in vitro and in vivo models of neurodegeneration and cerebral ischemia : potential involvement of the endogenous globins of the system central nervous system

Le stress oxydatif joue un rôle majeur dans la mort des cellules neuronales dans diverses conditions neuropathologiques. Cependant, les astrocytes réactifs, en produisant des facteurs neuroprotecteurs et antioxydants, sont capables de protéger les neurones contre le stress oxydatif. De ce fait, la protection des cellules gliales contre les facteurs nocifs, s’avère indispensable pour prévenir les dommages des cellules nerveuses. Les globines du cerveau, en particulier, la neuroglobine (Ngb) et l’hémoglobine (Hb), exprimées dans les cellules nerveuses, jouent un rôle important dans le métabolisme de l’oxygène. Récemment, il a été démontré, que ces protéines exercent des effets neuroprotecteurs dans les modèles expérimentaux de maladies neurodégénératives. Cependant, aucun effet glioprotecteur n’a été rapporté. Les objectifs de ce travail de thèse sont, de mettre en évidence les effets protecteurs de l’Hb et la Ngb dans les astrocytes en culture en présence d’un stress oxydant, et d’élucider les mécanismes intracellulaires mis en jeu. Nous avons démontré que l’Hb et la Ngb sont capables de promouvoir la survie des astrocytes en condition de stress oxydatif, et ce en réduisant significativement la surproduction des ROS, la surexpression des gènes pro-inflammatoires (IL-6, IL-33, iNOS), le dysfonctionnement mitochondrial et la stimulation de l’activité de la caspase-3/7. Nous avons montré aussi que les effets anti-apoptotiques impliquent l’activation des voies de signalisation ERK-MAPK. En outre, nous avons vérifié les effets glioprotecteurs sur un modèle animal de stress oxydatif chronique, les souris KO TP53INP1, ainsi que sur un modèle animal d’hypoxie.Oxidative stress plays a major role in the death of neuronal cells under various neuropathological conditions. However, reactive astrocytes, by producing neuroprotective and antioxidant factors, are able to protect neurons against oxidative stress. Therefore, protecting glial cells from harmful factors is essential to prevent nerve cell damage. Brain globins, in particular, neuroglobin (Ngb) and hemoglobin (Hb), expressed in neurons and glial cells, play an important role in the metabolism of oxygen. Recently, it has been demonstrated that these proteins exert neuroprotective effects in experimental models of neurodegenerative diseases. However, no glioprotective effect has been reported. The objectives of this thesis work are to demonstrate the protective effects of Hb and Ngb in cultured astrocytes in the presence of oxidative stress and to elucidate the intracellular mechanisms involved. We have demonstrated That Hb and Ngb are able to promote the survival of astrocytes under oxidative stress conditions by significantly reducing over-production of ROS, overexpression of pro-inflammatory genes (IL-6, IL-33, iNOS) Mitochondrial dysfunction and stimulation of caspase-3/7 activity. We have also shown that anti-apoptotic effects involve the activation of ERK-MAPK signaling pathways. In addition, we verified the glioprotective effects on an animal model of chronic oxidative stress, KO mice TP53INP1, as well as on an animal model of hypoxia

Neuroglobin protects astroglial cells from hydrogen peroxide-induced oxidative stress and apoptotic cell death

International audienceOxidative stress, resulting from accumulation of reactive oxygen species, plays a critical role in astroglial cell death occurring in diverse neuropathological conditions. Numerous studies indicate that neuroglobin (Ngb) promotes neuron survival, but nothing is known regarding the action of Ngb in astroglial cell survival. Thus, the purpose of this study was to investigate the potential glioprotective effect of Ngb on hydrogen peroxide (H 2 O 2)-induced oxidative stress and apop-tosis in cultured mouse astrocytes. Incubation of cells with subnanomolar concentrations of Ngb (10 À14 –10 À10 M) was found to prevent both H 2 O 2-evoked reduction in surviving cells number and accumulation of reactive oxygen species in a concentration-dependent manner. Furthermore, Ngb treatment abolishes H 2 O 2-induced increase in mitochondrial oxygen consumption rates. Concomitantly, Ngb treatment rescues H 2 O 2-associated reduced expression of endogenous antiox-idant enzymes (superoxide dismutases and catalase) and prevents the stimulation of the expression of pro-inflammatory genes (inducible nitric oxide synthase, cyclooxygenase-2, and interleukin (IL) IL-6 and IL-33). Moreover, Ngb blocks the stimulation of Bax (pro-apoptotic) and the inhibition of Bcl-2 (anti-apoptotic) gene expression induced by H 2 O 2 , which in turn abolishes caspase 3 activation. The protective effect of Ngb upon H 2 O 2 induced activation of caspase 3 activity and cell death can be accounted for by activation of protein kinase A and mitogen-activated protein kinase transduction cascade. Finally, we demonstrate that Ngb increases Akt phosphoryla-tion and prevents H 2 O 2-provoked inhibition of ERK and Akt phosphorylation. Taken together, these data demonstrate for the first time that Ngb is a glioprotective agent that prevents H 2 O 2-induced oxidative stress and apoptotic astroglial cell death. Protection of astrocytes from oxidative insult may thus contribute to the neuroprotective effect of Ngb

Hemoglobin-Improved Protection in Cultured Cerebral Cortical Astroglial Cells: Inhibition of Oxidative Stress and Caspase Activation

International audienceOxidative stress plays a major role in triggering astroglial cell death in diverse neuropathological conditions such as ischemia and neurodegenerative diseases. Numerous studies indicate that hemoglobin (Hb) is expressed in both resting and reactive glia cells, but nothing is known regarding a possible role of Hb on astroglial cell survival. Thus, the purpose of the present study was to investigate the potential glioprotective effect of Hb on hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in cultured rat astrocytes. Our study demonstrates that administration of graded concentrations of Hb (10−12 to 10−6 M) to H2O2-treated astrocytes reduces cell death in a concentration-dependent manner. H2O2 treatment induces the accumulation of reactive oxygen species (ROS) and nitric oxide (NO), a drop of the mitochondrial membrane potential, and a stimulation of caspase-3/7 activity. Exposure of H2O2-treated cells to Hb was accompanied by marked attenuations of ROS and NO surproductions, mitochondrial membrane potential reduction, and caspase-3/7 activity increase. The protective action of Hb was blocked by the protein kinase A (PKA) inhibitor H89, the protein kinase C (PKC) inhibitor chelerythrine, and the mitogen-activated protein (MAP)-kinase kinase (MEK) inhibitor U0126. Taken together, these data demonstrate for the first time that Hb is a glioprotective factor that protects astrocytes from apoptosis induced by oxidative stress and suggest that Hb may confer neuroprotection in neurodegenerative diseases. The anti-apoptotic activity of Hb on astrocytes is mediated through the PKA, PKC, and MAPK transduction pathways and can be accounted for by inhibition of oxidative stress-induced mitochondrial dysfunctions and caspase activation

The impact of perceived corporate social responsibility on employee engagement: The case of Qatar

This work investigates the role of perceived corporate social responsibility (CSR) in enhancing employee engagement (EE) in the State of Qatar. We collected survey data from local and expatriate employees of government, semigovernment, and private sector Qatari firms on their perceptions of CSR activities undertaken by their employers. The scope of CSR included activities targeted at employees, customers, the government, and society at large (social and nonsocial stakeholders). We also measured two facets of EE: organizational engagement and job engagement. We examined how these dimensions of EE are associated with employees' perception of the firm's CSR activities, controlling for the type of firm (government, semigovernment, or private) and the type of employee (local or expatriate). Our results reveal that CSR to society and CSR to employees affect organizational engagement whereas CSR to customers affects job engagement. CSR to the government is not significantly related to either aspect of EE.Scopu

Effect of Gamma Irradiation on Pathogenic Staphylococcus aureus in Packaged Ready-to-Eat Salads Treated with Biological Extracts

Providing pathogen-free ready-to-eat (RTE) salads is critical for all consumers, especially individuals with weakened immunity. In this study, the efficacy of γ-irradiation on Staphylococcus aureus (S. aureus) in freshly packaged salads (4.24 log CFU/g) treated with essential oil (EO) and myrtle juice during 10 days of storage and their impact on organoleptic properties were investigated. EO was extracted by hydrodistillation and the chemical composition was analyzed by gas chromatography with Flame Ionization Detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS). Myrtle juice was prepared from fresh fruits. The cytotoxic effects of Thymus capitatus (T. capitatus) EO against a normal human umbilical vein endothelial cell (HUVEC) were assessed. GC/FID and GC–MS analysis of the thyme EO revealed the presence of 13 compounds, including carvacrol (79.55%) and p-cymene (7.93%) as major components. The EO was found to be noncytotoxic, with concentrations lower than 0.16 µL/mL. A reduction of more than 3 log CFU/g and a total inactivation of S. aureus were achieved with the combination of gamma irradiation at 0.5 kGy with myrtle juice at 6 µL/mL and EO at 0.08 µL/mL, respectively. The treatment of fresh RTE salads with thyme and myrtle juice was evaluated as acceptable by the sensory panel. The combined effect showed a synergistic potential on the inactivation of S. aureus

Effect of Benzothiadiazole and Salicylic Acid Resistance Inducers on Orobanche foetida Infestation in Vicia faba

The broomrape or orobanche (Orobanche foetida) is considered as an important agricultural problem of faba bean (Vicia faba var. minor) production in Tunisia. The effect of salicylic acid (SA) and benzothiadiazole (BTH) on the induction of faba bean resistance to O. foetida was studied. Three application methods (seed soaking, foliar spraying and watering) were used. SA and BTH treatments reduced broomrape infestation under controlled conditions in pot and Petri dish experiments. In pot experiment, SA and BTH treatments reduced broomrape total number. Seed soaking treatments were more effective than foliar spraying and watering. In Petri dish experiment, O. foetida seed germination and the number of orobanche tubercles were reduced. The most efficient method was watering for SA and BTH treatments. This reduction was associated to a delay in the tubercle formation. The different application methods of SA and BTH treatment attest that the induced systemic resistance to O. foetida can be used in integrated management of broomrapes