Automated Extraction Improves Multiplex Molecular Detection of Infection in Septic Patients

Sepsis is one of the leading causes of morbidity and mortality in hospitalized patients worldwide. Molecular technologies for rapid detection of microorganisms in patients with sepsis have only recently become available. LightCycler SeptiFast test Mgrade (Roche Diagnostics GmbH) is a multiplex PCR analysis able to detect DNA of the 25 most frequent pathogens in bloodstream infections. The time and labor saved while avoiding excessive laboratory manipulation is the rationale for selecting the automated MagNA Pure compact nucleic acid isolation kit-I (Roche Applied Science, GmbH) as an alternative to conventional SeptiFast extraction. For the purposes of this study, we evaluate extraction in order to demonstrate the feasibility of automation. Finally, a prospective observational study was done using 106 clinical samples obtained from 76 patients in our ICU. Both extraction methods were used in parallel to test the samples. When molecular detection test results using both manual and automated extraction were compared with the data from blood cultures obtained at the same time, the results show that SeptiFast with the alternative MagNA Pure compact extraction not only shortens the complete workflow to 3.57 hrs., but also increases sensitivity of the molecular assay for detecting infection as defined by positive blood culture confirmation

Deciduous and evergreen oaks show contrasting adaptive responses in leaf mass per area across environments

Increases in leaf mass per area (LMA) are commonly observed in response to environmental stresses and are achieved through increases in leaf thickness and/or leaf density. Here, we investigated how the two underlying components of LMA differ in relation to species native climates and phylogeny, across deciduous and evergreen species. Using a phylogenetic approach, we quantified anatomical, compositional and climatic variables from 40 deciduous and 45 evergreen Quercus species from across the Northern Hemisphere growing in a common garden. Deciduous species from shorter growing seasons tended to have leaves with lower LMA and leaf thickness than those from longer growing seasons, while the opposite pattern was found for evergreens. For both habits, LMA and thickness increased in arid environments. However, this shift was associated with increased leaf density in evergreens but reduced density in deciduous species. Deciduous and evergreen oaks showed fundamental leaf morphological differences that revealed a diverse adaptive response. While LMA in deciduous species may have diversified in tight coordination with thickness mainly modulated by aridity, diversification of LMA within evergreens appears to be dependent on the infrageneric group, with diversification in leaf thickness modulated by both aridity and cold, while diversification in leaf density is only modulated by aridity

Patients receiving a high burden of antibiotics in the community in Spain: a cross-sectional study

Some patients in the community receive a high burden of antibiotics. We aimed at describing the characteristics of these patients, antibiotics used, and conditions for which they received antibiotics. We carried out a cross-sectional study. Setting: Thirty Health Primary Care Areas from 12 regions in Spain, covering 5, 960, 191 inhabitants. Patients having at least 30 packages of antibacterials for systemic use dispensed in 2017 were considered. Main outcome measures: Prevalence of antibiotic use, conditions for which antibiotics were prescribed, clinical characteristics of patients, comorbidities, concomitant treatments, and microbiological isolates. Patient''s average age was 70 years; 52% were men; 60% smokers/ex-smokers; 54% obese. Overall, 93% of patients had, at least, one chronic condition, and four comorbidities on average. Most common comorbidities were cardiovascular and/or hypertension (67%), respiratory diseases (62%), neurological/mental conditions (32%), diabetes (23%), and urological diseases (21%); 29% were immunosuppressed, 10% were dead at the time of data collection. Patients received three antibiotic treatments per year, mainly fluoroquinolones (28%), macrolides (21%), penicillins (19%), or cephalosporins (12%). Most frequently treated conditions were lower respiratory tract (infections or prophylaxis) (48%), urinary (27%), and skin/soft tissue infections (11%). Thirty-five percent have been guided by a microbiological diagnosis, being Pseudomonas aeruginosa (30%) and Escherichia coli (16%) the most frequent isolates. In conclusion, high antibiotic consumers in the community were basically elder, with multimorbidity and polymedication. They frequently received broad-spectrum antibiotics for long periods of time. The approach to infections in high consumers should be differentiated from healthy patients receiving antibiotics occasionally

First observation of Bs -> D_{s2}^{*+} X mu nu decays

Using data collected with the LHCb detector in proton-proton collisions at a centre-of-mass energy of 7 TeV, the semileptonic decays Bs -> Ds+ X mu nu and Bs -> D0 K+ X mu nu are detected. Two structures are observed in the D0 K+ mass spectrum at masses consistent with the known D^+_{s1}(2536) and $D^{*+}_{s2}(2573) mesons. The measured branching fractions relative to the total Bs semileptonic rate are B(Bs -> D_{s2}^{*+} X mu nu)/B(Bs -> X mu nu)= (3.3\pm 1.0\pm 0.4)%, and B(Bs -> D_{s1}^+ X munu)/B(Bs -> X mu nu)= (5.4\pm 1.2\pm 0.5)%, where the first uncertainty is statistical and the second is systematic. This is the first observation of the D_{s2}^{*+} state in Bs decays; we also measure its mass and width.Comment: 8 pages 2 figures. Published in Physics Letters

The syndrome of central hypothyroidism and macroorchidism: IGSF1 controls TRHR and FSHB expression by differential modulation of pituitary TGFβ and Activin pathways

IGSF1 (Immunoglobulin Superfamily 1) gene defects cause central hypothyroidism and macroorchidism. However, the pathogenic mechanisms of the disease remain unclear. Based on a patient with a full deletion of IGSF1 clinically followed from neonate to adulthood, we investigated a common pituitary origin for hypothyroidism and macroorchidism, and the role of IGSF1 as regulator of pituitary hormone secretion. The patient showed congenital central hypothyroidism with reduced TSH biopotency, over-secretion of FSH at neonatal minipuberty and macroorchidism from 3 years of age. His markedly elevated inhibin B was unable to inhibit FSH secretion, indicating a status of pituitary inhibin B resistance. We show here that IGSF1 is expressed both in thyrotropes and gonadotropes of the pituitary and in Leydig and germ cells in the testes, but at very low levels in Sertoli cells. Furthermore, IGSF1 stimulates transcription of the thyrotropin-releasing hormone receptor (TRHR) by negative modulation of the TGFβ1-Smad signaling pathway, and enhances the synthesis and biopotency of TSH, the hormone secreted by thyrotropes. By contrast, IGSF1 strongly down-regulates the activin-Smad pathway, leading to reduced expression of FSHB, the hormone secreted by gonadotropes. In conclusion, two relevant molecular mechanisms linked to central hypothyroidism and macroorchidism in IGSF1 deficiency are identified, revealing IGSF1 as an important regulator of TGFβ/Activin pathways in the pituitary

Powder Compaction: Compression Properties of Cellulose Ethers

Effective development of matrix tablets requires a comprehensive understanding of different raw material attributes and their impact on process parameters. Cellulose ethers (CE) are the most commonly used pharmaceutical excipients in the fabrication of hydrophilic matrices. The innate good compression and binding properties of CE enable matrices to be prepared using economical direct compression (DC) techniques. However, DC is sensitive to raw material attributes, thus, impacting the compaction process. This article critically reviews prior knowledge on the mechanism of powder compaction and the compression properties of cellulose ethers, giving timely insight into new developments in this field

A new method to detect globular clusters with the S-PLUS survey

In this paper, we describe a new method to select globular cluster (GC) candidates, including galaxy subtraction with unsharp masking, template fitting techniques, and the inclusion of Gaia’s proper motions. We report the use of the 12-band photometric system used by S-PLUS to determine radial velocities and stellar populations of GCs around nearby galaxies. Specifically, we assess the effectiveness of identifying GCs around nearby and massive galaxies (D 200 km s-1) in a multiband survey such as S-PLUS by using spectroscopically confirmed GCs and literature GC candidate lists around the bright central galaxy in the Fornax cluster, NGC 1399 (D = 19 Mpc), and the isolated lenticular galaxy NGC 3115 (D = 9.4 Mpc). Despite the shallow survey depth, which limits this work to r < 21.3 mag, we measure reliable photometry and perform robust SED fitting for a sample of 115 GCs around NGC 1399 and 42 GCs around NGC 3115, recovering radial velocities, ages, and metallicities for the GC populations. © 2021 The Author(s).MLB and CMdO acknowledge the financial support of the Sao Paulo Research Foundation (FAPESP) under grant 2019/23388-0. CEB acknowledges FAPESP, grant2016/12331-0. PC acknowledges support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) under grant 310041/2018-0. DdBS also acknowledges Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) process number 2017/00204-6 for the financial support. AC-S acknowledge funding from the brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação de Amparo à Pesquisa do Estado do RS (FAPERGS) through grants CNPq-403580/2016-1, CNPq-11153/2018-6, PqG/FAPERGS-17/2551-0001, FAPERGS/CAPES 19/2551-0000696-9, L’Oréal UNESCO ABC Para Mulheres na Ciência and the Chinese Academy of Sciences (CAS) President’s International Fellowship Initiative (PIFI) through grant E085201009. AA-C acknowledges support from the State Agency for Research of the Spanish MCIU through the ‘Center of Excellence Severo Ochoa’ award to the Instituto de Astrofísica de Andalucía (SEV-2017-0709). This work was funded with grants from Consejo Nacional de Investigaciones Científicas y Técnicas and Universidad Nacional de La Plata (Argentina). The S-PLUS project, including the T80-South robotic telescope and the S-PLUS scientific survey, was founded as a partnership between the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), the Observatório Nacional (ON), the Federal University of Sergipe (UFS), and the Federal University of Santa Catarina (UFSC), with important financial and practical contributions from other collaborating institutes in Brazil, Chile (Universidad de La Serena), and Spain (Centro de Estudios de Física del Cosmos de Aragón, CEFCA). We further acknowledge financial support from the São Paulo Research Foundation (FAPESP), the Brazilian National Research Council (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), the Carlos Chagas Filho Rio de Janeiro State Research Foundation (FAPERJ), and the Brazilian Innovation Agency (FINEP). The authors are grateful for the contributions from CTIO staff in helping in the construction, commissioning, and maintenance of the T80-South telescope and camera. We are also indebted to Rene Laporte and INPE, as well as Keith Taylor, for their important contributions to the project. We also thank CEFCA staff for their help with T80-South, specifically we thank Antonio Marín-Franch for his invaluable contributions in the early phases of the project, David Cristóbal-Hornillos and his team for their help with the installation of the data reduction package jype version 0.9.9, César Íãiguez for providing 2D measurements of the filter transmissions, and all other staff members for their support.Peer reviewe

A new method to detect globular clusters with the S-PLUS survey

In this paper, we describe a new method to select globular cluster (GC) candidates, including galaxy subtraction with unsharp masking, template fitting techniques, and the inclusion of Gaia’s proper motions. We report the use of the 12-band photometric system used by S-PLUS to determine radial velocities and stellar populations of GCs around nearby galaxies. Specifically, we assess the effectiveness of identifying GCs around nearby and massive galaxies (D 200 km s-1) in a multiband survey such as S-PLUS by using spectroscopically confirmed GCs and literature GC candidate lists around the bright central galaxy in the Fornax cluster, NGC 1399 (D = 19 Mpc), and the isolated lenticular galaxy NGC 3115 (D = 9.4 Mpc). Despite the shallow survey depth, which limits this work to r < 21.3 mag, we measure reliable photometry and perform robust SED fitting for a sample of 115 GCs around NGC 1399 and 42 GCs around NGC 3115, recovering radial velocities, ages, and metallicities for the GC populations. © 2021 The Author(s).MLB and CMdO acknowledge the financial support of the Sao Paulo Research Foundation (FAPESP) under grant 2019/23388-0. CEB acknowledges FAPESP, grant2016/12331-0. PC acknowledges support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) under grant 310041/2018-0. DdBS also acknowledges Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) process number 2017/00204-6 for the financial support. AC-S acknowledge funding from the brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação de Amparo à Pesquisa do Estado do RS (FAPERGS) through grants CNPq-403580/2016-1, CNPq-11153/2018-6, PqG/FAPERGS-17/2551-0001, FAPERGS/CAPES 19/2551-0000696-9, L’Oréal UNESCO ABC Para Mulheres na Ciência and the Chinese Academy of Sciences (CAS) President’s International Fellowship Initiative (PIFI) through grant E085201009. AA-C acknowledges support from the State Agency for Research of the Spanish MCIU through the ‘Center of Excellence Severo Ochoa’ award to the Instituto de Astrofísica de Andalucía (SEV-2017-0709). This work was funded with grants from Consejo Nacional de Investigaciones Científicas y Técnicas and Universidad Nacional de La Plata (Argentina). The S-PLUS project, including the T80-South robotic telescope and the S-PLUS scientific survey, was founded as a partnership between the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), the Observatório Nacional (ON), the Federal University of Sergipe (UFS), and the Federal University of Santa Catarina (UFSC), with important financial and practical contributions from other collaborating institutes in Brazil, Chile (Universidad de La Serena), and Spain (Centro de Estudios de Física del Cosmos de Aragón, CEFCA). We further acknowledge financial support from the São Paulo Research Foundation (FAPESP), the Brazilian National Research Council (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), the Carlos Chagas Filho Rio de Janeiro State Research Foundation (FAPERJ), and the Brazilian Innovation Agency (FINEP). The authors are grateful for the contributions from CTIO staff in helping in the construction, commissioning, and maintenance of the T80-South telescope and camera. We are also indebted to Rene Laporte and INPE, as well as Keith Taylor, for their important contributions to the project. We also thank CEFCA staff for their help with T80-South, specifically we thank Antonio Marín-Franch for his invaluable contributions in the early phases of the project, David Cristóbal-Hornillos and his team for their help with the installation of the data reduction package jype version 0.9.9, César Íãiguez for providing 2D measurements of the filter transmissions, and all other staff members for their support.Peer reviewe

Diverse Applications of Nanomedicine

The design and use of materials in the nanoscale size range for addressing medical and health-related issues continues to receive increasing interest. Research in nanomedicine spans a multitude of areas, including drug delivery, vaccine development, antibacterial, diagnosis and imaging tools, wearable devices, implants, high-throughput screening platforms, etc. using biological, nonbiological, biomimetic, or hybrid materials. Many of these developments are starting to be translated into viable clinical products. Here, we provide an overview of recent developments in nanomedicine and highlight the current challenges and upcoming opportunities for the field and translation to the clinic. \ua9 2017 American Chemical Society