Discriminating between viable and membrane-damaged cells of the plant pathogen Xylella fastidiosa.

Xylella fastidiosa is a plant pathogenic bacterium with devastating consequences to several crops of economic importance across the world. While this pathogen has been studied for over a century in the United States, several aspects of its biology remain to be investigated. Determining the physiological state of bacteria is essential to understand the effects of its interactions with different biotic and abiotic factors on cell viability. Although X. fastidiosa is culturable, its slow growing nature makes this technique cumbersome to assess the physiological state of cells present in a given environment. PMA-qPCR, i.e. the use of quantitative PCR combined with the pre-treatment of cells with the dye propidium monoazide, has been successfully used in a number of studies on human pathogens to calculate the proportion of viable cells, but has less frequently been tested on plant pathogens. We found that the use of a version of PMA, PMAxx, facilitated distinguishing between viable and non-viable cells based on cell membrane integrity in vitro and in planta. Additional experiments comparing the number of culturable, viable, and total cells in planta would help further confirm our initial results. Enhancers, intended to improve the efficacy of PMAxx, were not effective and appeared to be slightly toxic to X. fastidiosa

Asymmetries of the Stokes V profiles observed by HINODE SOT/SP in the quiet Sun

We present the first classification of SOT/SP circular polarization measurements with the aim of highlighting exhaustively the whole variety of Stokes V shapes emerging from the quiet Sun. k-means is used to classify HINODE SOT/SP Stokes V profiles observed in the quiet Sun network and internetwork (IN). We analyze a 302 x 162 square arcsec field-of-view (FOV) which can be considered a complete sample of quiet Sun measurements performed at at the disk center with 0.32 arcsec angular resolution and 0.001 polarimetric sensitivity. Such a classification allows us to divide the whole dataset in classes, with each class represented by a cluster profile, i.e., the average of the profiles in the class. The set of 35 cluster profiles derived from the analysis completely characterizes SOT/SP quiet Sun measurements. The separation between network and IN profile shapes is evident - classes in the network are not present in the IN, and vice versa. Asymmetric profiles are approximatively 93 % of the total number of profiles. Among these, approximatively 34 % of the profiles are strongly asymmetric profiles, and they can be divided in three families: blue-lobe, red-lobe, and Q-like profiles. The blue-lobe profiles tend to be associated with upflows (granules), whereas the red-lobe and Q-like ones appear in downflows (intergranular lanes). Such profiles need to be interpreted considering model atmospheres different from a uniformly magnetized Milne-Eddington (ME) atmosphere, i.e., characterized by gradients and/or discontinuities in the magnetic field and velocity along the line-of-sight (LOS).Comment: 11 pages, 4 figures, accepted for publication in Astronomy and Astrophysic

The Efficacy of Therapeutic Respiratory Muscle Training Interventions in People with Bronchiectasis: A Systematic Review and Meta-Analysis

Background: Respiratory muscle dysfunction is an important health problem with high morbidity and mortality and associated costs in patients with bronchiectasis (BC). The aim of this study was to analyse the effects of therapeutic respiratory muscle training (RMT) interventions on improving sputum clearance, ventilator function, muscle strength and functional capacity in BC. Methods: Systematic review and meta-analysis were conducted following PRISMA guidelines. Two independent investigators searched using several electronic databases. The methodological quality of nine studies was assessed using the PEDro scale. Study selection/eligibility criteria: The following were included: randomised controlled trials, randomised crossover trials and pilot studies of patients with BC that used the intervention as RMT (inspiratory/expiratory) and evaluations of respiratory muscle strength (maximal expiratory pressure/maximal inspiratory pressure). This systematic review was registered in PROSPERO (CRD42017075101). Nine studies were included, five of which obtained an A recommendation grade, three with B, and one with C. Study quality was poor to good (mean PEDro Score of 6.375 out of 10). Studies had small sample sizes (8-98). Results show improvements on PImax in favour of therapeutic respiratory muscle training intervention (MD = 6.08; 95% CI = 1.38, 10.77; p < 0.01; I-2 = 92%). However, high heterogeneity was identified on meta-analysis

Plasma free amino acid profiles of Boer goat bucks as influenced by two feeding regimens

The aim of the study was to monitor the physiological changes in plasma free amino acid profiles in Boer goats under nutritional restriction. Fifteen Boer goat bucks were allocated to two experimental groups: CG (control), fed Themeda trianda hay, supplemented with maize, molasses and urea ad libitum; and RG (restricted feeding) fed the same hay (80 and 86% of the daily NRC energy and protein requirements, respectively) for 29 days. Animals were weighed and blood collected weekly for determination of plasma free amino acid profiles and the results compared between experimental groups. At the end of the experimental period the CG animals had higher concentrations of alanine, tyrosine and citrulline amino acids. The RG group had higher concentrations of valine, isoleucine, leucine, threonine, methionine, lysine, taurine, ornithine, hydroxyproline and tri-methyl-histidine, while glycine, serine, aspartic acid, glutamic acid, arginine, histidine and proline levels were similar in both groups. From the results it can be concluded that plasma amino acid profiles in the Boer goat were significantly affected by nutritional restriction. The degradation of short carbon chain amino acids is preferred to long carbon chain amino acids for gluconeogenesis in the physiological mechanisms of response to nutritional restriction in Boer goats. Keywords: Boer goat; nutrition; restricted feeding; amino acids South African Journal of Animal Science Vol. 36 (5) 2006: pp.14-1

Caracterização de Doentes com Espondilite Anquilosante em Hidrocinesiterapia – Uma Avaliação Multidimensional

OBJECTIVES: Clinical, functional and working characterization of an Ankylosing Spondylitis (AS) group of patients that perform hydrotherapy regularly in a physical and rehabilitation department. Assessment of the benefit of hydrotherapy in symptom relief, function and consumption of analgesics and non-steroidal anti-inflammatory drugs (NSAIDs). MATERIAL AND METHODS: A transversal characterization of a group of patients with SA undergoing hydrotherapy was performed. Demographic, clinical (including disease activity, function and health-related quality of life), radiographic and laboratorial data was collected. A questionnaire about working situation, presence of dyspnoea, smoking, number of sessions and benefit of hydrotherapy was applied. RESULTS: 22 patients (73% males) were enrolled in the study, mean age 55.6 ± 8.8 years, mean duration of the disease 28.0 ± 13.13 years. Apart from the axial involvement, 50% had a previous history of enthesitis, 54.5% peripheral arthritis, 18% dactylitis and 36% uveitis. At the day of assessment, 81% had low-back pain complaints (39% inflammatory rhythm), 18% oligoarthritis, 9.1% had total hip and/or knee prosthesis. The majority of the patients had active disease, accentuated functional deterioration and reduced health related quality of life. About 54.5% were retired due to disability, 18.2% were smokers and 36.4% had dyspnoea; 31.8% presented restrictive ventilatory alterations; 36.4% obstructive (predominance in the small airways); 13.6% mixed and in 18.2% the spiromety was normal. The mean total time of hydrotherapy was 13 ± 6.8 years. The patients attended a mean of 3 sessions per week and 3 series of 15 sessions per year. Of the 22.7% that performed another physical activity, 80% walked and 20% cycled. The ingestions of analgesics (p < 0.05) and NSAIDs (p < 0.01) were also reduced. CONCLUSION: A high percentage of spyrometric changes were identified. The majority of the patients are retired due to disability. The patients feel benefit with hydrotherapy, that contributed to reduction of the analgesic and NSAIDs consumption. The importance of the global systemic evaluation and multidisciplinary of the SA to optimize the therapeuthics and improve the quality of life of the patients is pointed out

Association between sleep-disordered breathing and breast cancer aggressiveness

Background Sleep-disordered breathing (SDB) has been associated with cancer aggressiveness, but studies focused on specific tumors are lacking. In this pilot study we investigated whether SDB is associated with breast cancer (BC) aggressiveness. Methods 83 consecutive women <65 years diagnosed with primary BC underwent a home respiratory polygraphy. Markers of SDB severity included the apnea-hypopnea index (AHI) and the 4% oxygen desaturation index (ODI4). The Ki67 proliferation index, lack of hormone receptors (HR-), Nottingham Histological Grade (NHG), and tumor stage were used as markers of BC aggressiveness. The association between SDB and molecular subtypes of BC was also assessed. Results The mean (SD) age was 48.8 (8.8) years and body mass index was 27.4 (5.4) Kg/m2. 42 women (50.6%) were post-menopausal. The median (IQR) AHI was 5.1 (2–9.4), and ODI4 was 1.5 (0.5–5.8). The median (IQR) AHI did not differ between the groups with Ki67>28% and Ki6728% and Ki67<29% (51.2% vs 52.3%, p = 0.90), HR- and HR+ (58.3% vs 49.1%, p = 0.47), NHG categories (p = 0.89), different tumor stages (p = 0.71), or molecular subtypes (p = 0.73). These results did not change when the ODI4 was used instead of the AHI. Conclusion Our results do not support an association between the presence or severity of SDB and BC aggressiveness.Asociación de Neumología y Cirugía Torácica del Sur (NEUMOSUR) 1/201

A Visual Zooarchaeology Guide for Florida Artifacts

One key factor of archeology research is discovering the faunal bones at excavation sites and seeing what lived many years ago. Faunal remains are bones, hair, shells, and more, any piece of animal anatomy. Discovering the different types of bones at excavations in Florida can give archeologists a sense of the different species in this state that were utilized by humans in the past. This study is creating a visual guide and key for faunal bone artifacts in south Florida. The key will consist of photos of bone artifacts excavated in south Florida of different types of fish and other animals. Also, the key will describe the bones excavated so that people and scientists can know what the defining characteristics of different bones are. Creating this key will be helpful for any other researcher to teach how to identify archaeological bone

Diseño de recursos didácticos para aulas virtuales

1 archivo PDF (21 páginas)Texto que hace referencia a las dificultades a las que se enfrentan los docentes de ciencias básicas al utilizar plataformas a distancia, al diseñar y utilizar cursos, ya sea en e-learning o enseñanza combinada, y la forma en que se puede desarrollar la habilidad de resolver problemas, pues existen numerosas dificultades y obstáculos en este proceso, incluso en enseñanza presencial