Using high resolution tracer data to constrain water storage, flux and age estimates in a spatially distributed rainfall-runoff model

Acknowledgements The authors would like to thank Jonathan Dick, Josie Geris, Jason Lessels and Claire Tunaley for data collection and Audrey Innes for lab sample preparation. We also thank Christian Birkel for discussions about the model structure and comments on an earlier draft of the paper. Climatic data were provided by Iain Malcolm and Marine Scotland Fisheries at the Freshwater Lab, Pitlochry. Additional precipitation data were provided by the UK Meteorological Office and the British Atmospheric Data Centre (BADC). We thank the European Research Council ERC (project GA 335910 VEWA) for funding the VeWa project.Peer reviewedPublisher PD

The effects of diffuse ionized gas and spatial resolution on metallicity gradients: TYPHOON two-dimensional spectrophotometry of M83

We present a systematic study of the diffuse ionized gas (DIG) in M83 and its effects on the measurement of metallicity gradients at varying resolution scales. Using spectrophotometric data cubes of M83 obtained at the 2.5m duPont telescope at Las Campanas Observatory as part of the TYPHOON programme, we separate the H II regions from the DIG using the [S II]/H α ratio, HIIPHOT (H II-finding algorithm), and the H α surface brightness. We find that the contribution to the overall H α luminosity is approximately equal for the H II and DIG regions. The data is then rebinned to simulate low-resolution observations at varying resolution scales from 41 pc up to 1005 pc. Metallicity gradients are measured using five different metallicity diagnostics at each resolution. We find that all metallicity diagnostics used are affected by the inclusion of DIG to varying degrees. We discuss the reasons why the metallicity gradients are significantly affected by DIG using the H II dominance and emission line ratio radial profiles. We find that applying the [S II]/H α cut will provide a closer estimate of the true metallicity gradient up to a resolution of 1005 pc for all metallicity diagnostics used in this study.Parts of this research were conducted by the Australian Research Council Centre of Excellence for All Sky Astrophysics in 3 Dimensions (ASTRO 3D), through project number CE170100013. BG gratefully acknowledges the support of the Australian Research Council as the recipient of a Future Fellowship (FT140101202)

Beyond antibiotic prescribing rates: first-line antibiotic selection, prescription duration, and associated factors for respiratory encounters in urgent care

Abstract Objective: Assess urgent care (UC) clinician prescribing practices and factors associated with first-line antibiotic selection and recommended duration of therapy for sinusitis, acute otitis media (AOM), and pharyngitis. Design: Retrospective cohort study. Participants: All respiratory UC encounters and clinicians in the Intermountain Health (IH) network, July 1st, 2019–June 30th, 2020. Methods: Descriptive statistics were used to characterize first-line antibiotic selection rates and the duration of antibiotic prescriptions during pharyngitis, sinusitis, and AOM UC encounters. Patient and clinician characteristics were evaluated. System-specific guidelines recommended 5–10 days of penicillin, amoxicillin, or amoxicillin-clavulanate as first-line. Alternative therapies were recommended for penicillin allergy. Generalized estimating equation modeling was used to assess predictors of first-line antibiotic selection, prescription duration, and first-line antibiotic prescriptions for an appropriate duration. Results: Among encounters in which an antibiotic was prescribed, the rate of first-line antibiotic selection was 75%, the recommended duration was 70%, and the rate of first-line antibiotic selection for the recommended duration was 53%. AOM was associated with the highest rate of first-line prescriptions (83%); sinusitis the lowest (69%). Pharyngitis was associated with the highest rate of prescriptions for the recommended duration (91%); AOM the lowest (51%). Penicillin allergy was the strongest predictor of non–first-line selection (OR = 0.02, 95% CI [0.02, 0.02]) and was also associated with extended duration prescriptions (OR = 0.87 [0.80, 0.95]). Conclusions: First-line antibiotic selection and duration for respiratory UC encounters varied by diagnosis and patient characteristics. These areas can serve as a focus for ongoing stewardship efforts

Use of alternate coreceptors on primary cells by two HIV-1 isolates

AbstractTwo HIV-1 isolates (CM4 and CM9) able to use alternate HIV-1 coreceptors on transfected cell lines were tested for their sensitivity to inhibitors of HIV-1 entry on primary cells. CM4 was able to use CCR5 and Bob/GPR15 efficiently in transfected cells. The R5 isolate grew in Δ32/Δ32 CCR5 PBMC in the absence or presence of AMD3100, a CXCR4-specific inhibitor, indicating that it uses a receptor other than CCR5 or CXCR4 on primary cells. It was insensitive to the CCR5 entry inhibitors RANTES and PRO140, but was partially inhibited by vMIP-1, a chemokine that binds CCR3, CCR8, GPR15 and CXCR6. The coreceptor used by this isolate on primary cells is currently unknown. CM9 used CCR5, CXCR4, Bob/GPR15, CXCR6, CCR3, and CCR8 on transfected cells and was able to replicate in the absence or presence of AMD3100 in Δ32/Δ32 CCR5 PBMC. It was insensitive to eotaxin, vMIP-1 and I309 when tested individually, but was inhibited completely when vMIP-1 or I309 was combined with AMD3100. Both I309 and vMIP-1 bind CCR8, strongly suggesting that this isolate can use CCR8 on primary cells. Collectively, these data suggest that some HIV-1 isolates can use alternate coreceptors on primary cells, which may have implications for strategies that aim to block viral entry

Groundwater dynamics in a till hillslope: flow directions, gradients and delay

Knowledge of groundwater dynamics is important for the understanding of hydrological controls on chemical processes along the water flow pathways. To increase our knowledge of groundwater dynamics in areas with shallow groundwater, the groundwater dynamics along a hillslope were studied in a boreal catchment in Southern Sweden. The forested hillslope had a 1-2 m deep layer of sandy till above bedrock. The groundwater flow direction and slope were calculated under the assumption that the flow followed the slope of the groundwater table, which was computed for different triangles, each defined by three groundwater wells. The flow direction showed considerable variations over time, with a maximum variation of 75 degrees. During periods of high groundwater levels the flow was almost perpendicular to the stream, but as the groundwater level fell, the flow direction became gradually more parallel to the stream, directed in the downstream direction. These findings are of importance for the interpretation of results from hillslope transects, where the flow direction usually is assumed to be invariable and always in the direction of the hillslope. The variations in the groundwater flow direction may also cause an apparent dispersion for groundwater-based transport. In contrast to findings in several other studies, the groundwater level was most responsive to rainfall and snowmelt in the upper part of the hillslope, while the lower parts of the slope reached their highest groundwater level up to 40 hours after the upper parts. This can be explained by the topography with a wetter hollow area in the upper part

Response of Bortezomib Chemotherapy in Hepatic Amyloidosis

Amyloidosis is a rare disorder with a wide spectrum of presentations and anomalies. It is subdivided into 2 broad categories based on protein deposition; primary and secondary amyloidosis. It can present as a single-organ involvement or as a diffuse infiltrative multi-organ process. Isolated hepatic amyloidosis presentation is a rare phenomenon that develops due to insoluble amyloid deposition in liver. Its clinical presentation is usually vague and ranges from mild hepatomegaly with elevated liver enzymes to acute liver failure and hepatic rupture. Currently, there are scarce data available regarding treatment options for biopsy-proven hepatic amyloidosis. In this review article, we present an interesting case of hepatic amyloidosis and its poor outcome to new molecular targeted chemotherapy. Furthermore, we aim to review current and future diagnostic tools for early detection and advancements in targeted chemotherapeutics options available for hepatic amyloidosis

Multimodal imaging reveals the spatiotemporal dynamics of recollection.

Functional MRI research suggests that different frontal and parietal cortical regions support strategic processes that are engaged at different stages of recollection, from pre-retrieval processing of a cue to post-retrieval maintenance and evaluation of recollected information. Whereas some of these regions respond in a domain-general way, other regions are sensitive to the type of information being recollected. However, the low temporal resolution of fMRI cannot distinguish component processes at the time-scale at which recollection occurs. We therefore combined fMRI with the excellent temporal resolution of source localised EEG/MEG to investigate the spatiotemporal neural dynamics of recollection. fMRI and EEG/MEG data were collected from the same participants in two sessions while they retrieved different types of episodic information. This multimodal imaging approach revealed striking consistency between the regions identified with fMRI and EEG/MEG, providing novel evidence of how these brain areas interact over time to support source recollection. For domain-general recollection, results from both modalities converged in showing the strongest activations in medial parietal cortex, which according to EEG/MEG was reliable at a late retrieval stage. Domain-specific source recollection increased fMRI and EEG/MEG activation in the left lateral prefrontal cortex, which EEG/MEG indicated also to be recruited during a post-recollection stage. The findings suggest that although medial parietal and left lateral prefrontal regions mediate functionally different retrieval processes, they are both engaged at a late stage of episodic retrieval

Beyond antibiotic prescribing rates: first-line antibiotic selection, prescription duration, and associated factors for respiratory encounters in urgent care

OBJECTIVE: Assess urgent care (UC) clinician prescribing practices and factors associated with first-line antibiotic selection and recommended duration of therapy for sinusitis, acute otitis media (AOM), and pharyngitis. DESIGN: Retrospective cohort study. PARTICIPANTS: All respiratory UC encounters and clinicians in the Intermountain Health (IH) network, July 1st, 2019-June 30th, 2020. METHODS: Descriptive statistics were used to characterize first-line antibiotic selection rates and the duration of antibiotic prescriptions during pharyngitis, sinusitis, and AOM UC encounters. Patient and clinician characteristics were evaluated. System-specific guidelines recommended 5-10 days of penicillin, amoxicillin, or amoxicillin-clavulanate as first-line. Alternative therapies were recommended for penicillin allergy. Generalized estimating equation modeling was used to assess predictors of first-line antibiotic selection, prescription duration, and first-line antibiotic prescriptions for an appropriate duration. RESULTS: Among encounters in which an antibiotic was prescribed, the rate of first-line antibiotic selection was 75%, the recommended duration was 70%, and the rate of first-line antibiotic selection for the recommended duration was 53%. AOM was associated with the highest rate of first-line prescriptions (83%); sinusitis the lowest (69%). Pharyngitis was associated with the highest rate of prescriptions for the recommended duration (91%); AOM the lowest (51%). Penicillin allergy was the strongest predictor of non-first-line selection (OR = 0.02, 95% CI [0.02, 0.02]) and was also associated with extended duration prescriptions (OR = 0.87 [0.80, 0.95]). CONCLUSIONS: First-line antibiotic selection and duration for respiratory UC encounters varied by diagnosis and patient characteristics. These areas can serve as a focus for ongoing stewardship efforts