Variation in Assignment of the COPD Patients into a GOLD Group According to Symptoms Severity.

Introduction: The Global Organization of Lung Disease (GOLD) classifies patients with chronic obstructive pulmonary disease (COPD) taking into account the symptoms. The modified Medical Research Council’s dyspnea scale (mMRC) and the COPD assessment test (CAT) are used to assess these symptoms. In this study, we analyze the concordance of GOLD classification using mMRC and CAT. Patients and Methods: This is an observational study of a cohort of 169 patients with COPD, who were classified following the GOLD 2017 recommendations, using both mMRC and CAT. A concordance analysis was applied, and a ROC curve was generated to identify the CAT score that best concorded with the mMRC scale. Results: The concordance for the GOLD groups classified by CAT and mMRC was moderate (kappa 0.492). For mMRC score of 1 and 2, a CAT score of ≥9 and ≥16 showed the maximum value of the Youden index, respectively. By reclassifying the patients with the new cut-off points obtained, the best concordance was obtained between the cut-off point for CAT of 16 and for mMRC of 2, followed by CAT of 9 and mMRC of 1. Conclusion: Because of the deficient concordance between CAT and mMRC, we propose the use of new cut-off points in future updates of the GOLD strategy.post-print2909 K

An Overview of the IberSpeech-RTVE 2022 Challenges on Speech Technologies

Evaluation campaigns provide a common framework with which the progress of speech technologies can be effectively measured. The aim of this paper is to present a detailed overview of the IberSpeech-RTVE 2022 Challenges, which were organized as part of the IberSpeech 2022 conference under the ongoing series of Albayzin evaluation campaigns. In the 2022 edition, four challenges were launched: (1) speech-to-text transcription; (2) speaker diarization and identity assignment; (3) text and speech alignment; and (4) search on speech. Different databases that cover different domains (e.g., broadcast news, conference talks, parliament sessions) were released for those challenges. The submitted systems also cover a wide range of speech processing methods, which include hidden Markov model-based approaches, end-to-end neural network-based methods, hybrid approaches, etc. This paper describes the databases, the tasks and the performance metrics used in the four challenges. It also provides the most relevant features of the submitted systems and briefly presents and discusses the obtained results. Despite employing state-of-the-art technology, the relatively poor performance attained in some of the challenges reveals that there is still room for improvement. This encourages us to carry on with the Albayzin evaluation campaigns in the coming years.This work was partially supported by Radio Televisión Española through the RTVE Chair at the University of Zaragoza, and Red Temática en Tecnologías del Habla (RED2022-134270-T), funded by AEI (Ministerio de Ciencia e Innovación); It was also partially funded by the European Union’s Horizon 2020 research and innovation program under Marie Skłodowska-Curie Grant 101007666; in part by MCIN/AEI/10.13039/501100011033 and by the European Union “NextGenerationEU”/ PRTR under Grants PDC2021-120846C41 PID2021-126061OB-C44, and in part by the Government of Aragon (Grant Group T3623R); it was also partially funded by the Spanish Ministry of Science and Innovation (OPEN-SPEECH project, PID2019-106424RB-I00) and by the Basque Government under the general support program to research groups (IT-1704-22), and by projects RTI2018-098091-B-I00 and PID2021-125943OB-I00 (Spanish Ministry of Science and Innovation and ERDF) as well

Enhanced detection of viral RNA species using fokI-assisted digestion of DNA duplexes and DNA/RNA hybrids

The accurate detection of nucleic acids from certain biological pathogens is critical for the diagnosis of human diseases. However, amplified detection of RNA molecules from a complex sample by direct detection of RNA/DNA hybrids remains a challenge. Here, we show that type IIS endonuclease FokI is able to digest DNA duplexes and DNA/RNA hybrids when assisted by a dumbbell-like fluorescent sensing oligonucleotide. As proof of concept, we designed a battery of sensing oligonucleotides against specific regions of the SARS-CoV-2 genome and interrogated the role of FokI relaxation as a potential nicking enzyme for fluorescence signal amplification. FokI-assisted digestion of SARS-CoV-2 probes increases the detection signal of ssDNA and RNA molecules and decreases the limit of detection more than 3.5-fold as compared to conventional molecular beacon approaches. This cleavage reaction is highly specific to its target molecules, and no detection of other highly related B-coronaviruses was observed in the presence of complex RNA mixtures. In addition, the FokI-assisted reaction has a high multiplexing potential, as the combined detection of different viral RNAs, including different SARS-CoV-2 variants, was achieved in the presence of multiple combinations of fluorophores and sensing oligonucleotides. When combined with isothermal rolling circle amplification technologies, FokI-assisted digestion reduced the detection time of SARS-CoV-2 in COVID-19-positive human samples with adequate sensitivity and specificity compared to conventional reverse transcription polymerase chain reaction approaches, highlighting the potential of FokI-assisted signal amplification as a valuable sensing mechanism for the detection of human pathogens.Funding was provided by the ISCIII (COV00624 to J.R.T. andM.F.F., PI18/01527 and PI21/01067 to M.F.F.), CSIC (202020E092 to M.F.F), the European Commission NextGenerationEU, through CSIC’s Global Health Platform (PTI Salud Global) and the Spanish Ministry of Science and Innovation through the Recovery, Transformation and Resilience Plan (GL2021-03-39 and GL2021-03-040), the PCTI from the Asturias Government, co-funded by 2018−2022/FEDER (IDI/2018/146 to M.F.F.), the AECC (PROYE18061FERN to M.F.F), ISPA-Janssen (048-Intramural Nov-Tevar to J.R.T.) and the IUOPA. J.R.T is supported by a JdC fellowship from the Spanish Ministry of Science and Innovation (IJC2018-36825-I). R.F.P. and P.S.O. are supported by the Severo Ochoa program (BP17-114 and BP17-165). A.P. is supported by the PFIS program (ISCIII, FI19/ 00085). J.J.A.L. is supported by the AECC fellowship. C.M. and V.L. are supported by IUOPA, and R.G.U. is supported by CIBERER.Peer reviewe

Early high-titer plasma therapy to prevent severe Covid-19 in older adults

BACKGROUND: Therapies to interrupt the progression of early coronavirus disease 2019 (Covid-19) remain elusive. Among them, convalescent plasma administered to hospitalized patients has been unsuccessful, perhaps because antibodies should be administered earlier in the course of illness. METHODS We conducted a randomized, double-blind, placebo-controlled trial of convalescent plasma with high IgG titers against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in older adult patients within 72 hours after the onset of mild Covid-19 symptoms. The primary end point was severe respiratory disease, defined as a respiratory rate of 30 breaths per minute or more, an oxygen saturation of less than 93% while the patient was breathing ambient air, or both. The trial was stopped early at 76% of its projected sample size because cases of Covid-19 in the trial region decreased considerably and steady enrollment of trial patients became virtually impossible. RESULTS A total of 160 patients underwent randomization. In the intention-to-treat population, severe respiratory disease developed in 13 of 80 patients (16%) who received convalescent plasma and 25 of 80 patients (31%) who received placebo (relative risk, 0.52; 95% confidence interval [CI], 0.29 to 0.94; P = 0.03), with a relative risk reduction of 48%. A modified intention-to-treat analysis that excluded 6 patients who had a primary end-point event before infusion of convalescent plasma or placebo showed a larger effect size (relative risk, 0.40; 95% CI, 0.20 to 0.81). No solicited adverse events were observed. CONCLUSIONS Early administration of high-titer convalescent plasma against SARS-CoV-2 to mildly ill infected older adults reduced the progression of Covid-19. (Funded by the Bill and Melinda Gates Foundation and the Fundación INFANT Pandemic Fund; Dirección de Sangre y Medicina Transfusional del Ministerio de Salud number, PAEPCC19, Plataforma de Registro Informatizado de Investigaciones en Salud number, 1421, and ClinicalTrials.gov number, NCT04479163.).Fil: Libster, Romina Paula. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Pérez Marc, Gonzalo. Hospital Militar Central, Buenos Aires; ArgentinaFil: Wappner, Diego. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Coviello, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Bianchi, Alejandra. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Braem, Virginia. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Esteban, Ignacio. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Caballero, Mauricio Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wood, Cristian. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Berrueta, Mabel. Hospital Militar Central; ArgentinaFil: Rondan, Aníbal. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Lescano, Gabriela Mariel. Hospital Dr. Carlos Bocalandro; ArgentinaFil: Cruz, Pablo. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Ritou, Yvonne. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Fernández Viña, Valeria Silvina. Hospital Simplemente Evita; ArgentinaFil: Álvarez Paggi, Damián Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Esperante, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ferreti, Adrián. Hospital Dr. Carlos Bocalandro; ArgentinaFil: Ofman, Gaston. University of Oklahoma; Estados UnidosFil: Ciganda, Álvaro. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal Especializado de Agudos y Cronicos San Juan de Dios.; ArgentinaFil: Rodriguez, Rocío. Hospital Simplemente Evita; ArgentinaFil: Lantos, Jorge. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Valentini, Ricardo. No especifíca;Fil: Itcovici, Nicolás. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Hintze, Alejandra. No especifíca;Fil: Oyarvide, M. Laura. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Etchegaray, Candela. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Neira, Alejandra. Instituto de Efectividad Clínica y Sanitaria; ArgentinaFil: Name, Ivonne. Instituto de Efectividad Clínica y Sanitaria; ArgentinaFil: Alfonso, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Swiss Medical Group; ArgentinaFil: López Castelo, Rocío. Centro de Educación Médica e Investigaciones Clínicas "Norberto Quirno"; ArgentinaFil: Caruso, Gisela. Hospital Militar Central; ArgentinaFil: Rapelius, Sofía. Hospital Militar Central; ArgentinaFil: Alvez, Fernando. Hospital Militar Central; ArgentinaFil: Etchenique, Federico. Hospital Militar Central; ArgentinaFil: Dimase, Federico. Hospital Militar Central; ArgentinaFil: Alvarez, Darío. Hospital Militar Central; ArgentinaFil: Aranda, Sofía S.. Hospital Militar Central; ArgentinaFil: Sánchez Yanotti, Clara Inés. Hospital Militar Central; ArgentinaFil: De Luca, Julián. Hospital Militar Central; ArgentinaFil: Jares Baglivo, Sofía. Hospital Militar Central; ArgentinaFil: Laudanno, Sofía. Fundación Hematológica Sarmiento; ArgentinaFil: Nowogrodzki, Florencia. Swiss Medical Group; ArgentinaFil: Larrea, Ramiro. Hospital Municipal San Isidro; ArgentinaFil: Silveyra, María. Hospital Militar Central; ArgentinaFil: Leberzstein, Gabriel. No especifíca;Fil: Debonis, Alejandra. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Molinos, Juan. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: González, Miguel. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Perez, Eduardo. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Kreplak, Nicolás. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Pastor Argüello, Susana. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Gibbons, Luz. Hospital Municipal de San Isidro; ArgentinaFil: Althabe, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; ArgentinaFil: Bergel, Eduardo. Sanatorio Sagrado Corazón; ArgentinaFil: Polack, Fernando Pedro. Provincia de Buenos Aires. Ministerio de Salud; Argentin

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

Detectives de alimentos: un trabajo en equipo para la investigación de toxiinfecciones alimentarias

Proyecto de Innovación Docente en el que los estudiantes realizan un trabajo cooperativo mediante el método del puzle para diseñar fichas descriptivas de patógenos de transmisión alimentaria y resolver un caso de toxiinfección alimentari

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Plant diversity patterns in neotropical dry forests and their conservation implications

This is the author accepted manuscript. The final version is available from American Association for the Advancement of Science via the DOI in this record.Seasonally dry tropical forests are distributed across Latin America and the Caribbean and are highly threatened, with less than 10% of their original extent remaining in many countries. Using 835 inventories covering 4660 species of woody plants, we show marked floristic turnover among inventories and regions, which may be higher than in other neotropical biomes, such as savanna. Such high floristic turnover indicates that numerous conservation areas across many countries will be needed to protect the full diversity of tropical dry forests. Our results provide a scientific framework within which national decision-makers can contextualize the floristic significance of their dry forest at a regional and continental scale.This paper is the result of the Latin American and Caribbean Seasonally Dry Tropical Forest Floristic Network (DRYFLOR), which has been supported at the Royal Botanic Garden Edinburgh by a Leverhulme Trust International Network Grant (IN-074). This work was also supported by the U.K. Natural Environment Research Council grant NE/I028122/1; Colciencias Ph.D. scholarship 529; Synthesys Programme GBTAF-2824; the NSF (NSF 1118340 and 1118369); the Instituto Humboldt (IAvH)–Red colombiana de investigación y monitoreo en bosque seco; the Inter-American Institute for Global Change Research (IAI; Tropi-Dry, CRN2-021, funded by NSF GEO 0452325); Universidad Nacional de Rosario (UNR); and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). The data reported in this paper are available at www.dryflor.info. R.T.P. conceived the study. M.P., A.O.-F., K.B.-R., R.T.P., and J.W. designed the DRYFLOR database system. K.B.-R. and K.G.D. carried out most analyses. K.B.-R. R.T.P., and K.G.D. wrote the manuscript with substantial input from A.D.-S., R.L.-P., A.O.-F., D.P., C.Q., and R.R. All the authors contributed data, discussed further analyses, and commented on various versions of the manuscript. K.B.-R. thanks G. Galeano who introduced her to dry forest research. We thank J. L. Marcelo, I. Huamantupa, C. Reynel, S. Palacios, and A. Daza for help with fieldwork and data entry in Peru

Seroprevalence of canine leptospirosis in a rural community in the municipality of cienaga de oro, Córdoba (Colombia)

La leptospirosis representa un problema con implicaciones en salud pública. En los caninos es una enfermedad no siempre diagnosticada, a pesar de su difusión y el rol que desempeñan éstos como reservorios de leptospiras patógenas, convirtiéndose en una importante fuente de infección hacia el humano y otras especies de interés zootécnico. Se hace necesario estudiar la epidemiología de la leptospirosis en la especie canina, para contribuir al diseño de estrategias de prevención y de control. Para determinar la seroprevalencia de Leptospira interrogans y los serovares más frecuentes, se adelantó un estudio epidemiológico transversal que incluyó una muestra de 70, caninos mayores de cuatro meses, existentes en la comunidad rural del corregimiento de Berástegui, municipio de Ciénaga de Oro (Córdoba) y por medio de la aglutinación microscópica (MAT,) implementado con 14 serovares. Se consideró un suero positivo cuando se observó aglutinación del 50%, en una dilución ≥ 1:100. La seroprevalencia de leptospirosis en la población canina fue del 47,14% y la mayor frecuencia fue para los serovares grippotyphosa, con el 37,14%, seguido de icterohaemorrhagiae y pomona, con el 34,29 y 25,71%, respectivamente. Al discriminar la seroprevalencia por el número de serovares, se encontró que el 10,01% de las infecciones ocurrió por un sólo serovar; el 5,72%, por dos; el 4,24%, por tres y el 27,17%, por cuatro o más serovares. Los patrones de coaglutinación más frecuentes fueron icterohaemorrhagiae–grippotyphosa. La mayor seropositividad, se determinó en los machos, por ser este el mayor grupo muestreado en la población canina evaluada. Estos resultados sugieren que la población canina puede ser un posible reservorio de leptospirosis en la zona de estudio; es necesario ejecutar estudios que permitan el aislamiento y la tipificación de los serovares prevalentes en esta región.Leptospirosis is a problem with public health implications. In dogs the disease is not always diagnosed, despite its diffusion and the role that the canines have as reservoirs of pathogenic leptospires, becoming an important source of infections for humans and species of zootechnical interest. It is necessary to study the epidemiology of canine leptospirosis in order to design strategies for prevention and control. To determine the seroprevalence of Leptospira interrognas and the most frequent serovars, an advanced cross sectional epidemiological study was carried out. Samples of 70 dogs over four months in the rural community of Berástegui township, municipality of Ciénaga de Oro, Córdoba, were analyzed through MAT with 14 serovars. A serum was considered positive with 50% of agglutination at a dilution ≥ 1:100 Seroprevalence of leptospirosis in the dog population was 47.14% and the highest frequency was for serovar gryppotyphosa with 37.14%, followed by icterohaemorrhagiae and pomona with 34.29% and 25.71% respectively. Seroprevalence, discriminated by the number of serotypes, was found in 10.01% of the infections that occurred by a single serovar, 5.72% for two, 4.24% for three and 27.17% for four or more serovars. A most frequent coagglutination pattern was grippotyphosa-icterohaemorrhagiae. The highest seroprevalences was found in males since it was the largest group in the canine population tested. These results suggest that the canine population may be a possible reservoir of leptospirosis in the studied area. It is necessary to conduct studies that permit the isolation and characterization of the serovars prevalent in this region.Incluye referencias bibliográfica

SEROPREVALENCIA DE LEPTOSPIROSIS CANINA EN UNA COMUNIDAD RURAL DEL MUNICIPIO DE CIÉNAGA DE ORO, CÓRDOBA (COLOMBIA) SEROPREVALENCE OF CANINE LEPTOSPIROSIS IN A RURAL COMMUNITY IN THE MUNICIPALITY OF CIENAGA DE ORO, CÓRDOBA (COLOMBIA)

La leptospirosis representa un problema con implicaciones en salud pública. En los caninos es una enfermedad no siempre diagnosticada, a pesar de su difusión y el rol que desempeñan éstos como reservorios de leptospiras patógenas, convirtiéndose en una importante fuente de infección hacia el humano y otras especies de interés zootécnico. Se hace necesario estudiar la epidemiología de la leptospirosis en la especie canina, para contribuir al diseño de estrategias de prevención y de control. Para determinar la seroprevalencia de Leptospira interrogans y los serovares más frecuentes, se adelantó un estudio epidemiológico transversal que incluyó una muestra de 70, caninos mayores de cuatro meses, existentes en la comunidad rural del corregimiento de Berástegui, municipio de Ciénaga de Oro (Córdoba) y por medio de la aglutinación microscópica (MAT,) implementado con 14 serovares. Se consideró un suero positivo cuando se observó aglutinación del 50%, en una dilución &#8805; 1:100. La seroprevalencia de leptospirosis en la población canina fue del 47,14% y la mayor frecuencia fue para los serovares grippotyphosa, con el 37,14%, seguido de icterohaemorrhagiae y pomona, con el 34,29 y 25,71%, respectivamente. Al discriminar la seroprevalencia por el número de serovares, se encontró que el 10,01% de las infecciones ocurrió por un sólo serovar; el 5,72%, por dos; el 4,24%, por tres y el 27,17%, por cuatro o más serovares. Los patrones de coaglutinación más frecuentes fueron icterohaemorrhagiae-grippotyphosa. La mayor seropositividad, se determinó en los machos, por ser este el mayor grupo muestreado en la población canina evaluada. Estos resultados sugieren que la población canina puede ser un posible reservorio de leptospirosis en la zona de estudio; es necesario ejecutar estudios que permitan el aislamiento y la tipificación de los serovares prevalentes en esta región.Leptospirosis is a problem with public health implications. In dogs the disease is not always diagnosed, despite its diffusion and the role that the canines have as reservoirs of pathogenic leptospires, becoming an important source of infections for humans and species of zootechnical interest. It is necessary to study the epidemiology of canine leptospirosis in order to design strategies for prevention and control. To determine the seroprevalence of Leptospira interrognas and the most frequent serovars, an advanced cross sectional epidemiological study was carried out. Samples of 70 dogs over four months in the rural community of Berástegui township, municipality of Ciénaga de Oro, Córdoba, were analyzed through MAT with 14 serovars. A serum was considered positive with 50% of agglutination at a dilution &#8805; 1:100 Seroprevalence of leptospirosis in the dog population was 47.14% and the highest frequency was for serovar gryppotyphosa with 37.14%, followed by icterohaemorrhagiae and pomona with 34.29% and 25.71% respectively. Seroprevalence, discriminated by the number of serotypes, was found in 10.01% of the infections that occurred by a single serovar, 5.72% for two, 4.24% for three and 27.17% for four or more serovars. A most frequent coagglutination pattern was grippotyphosa-icterohaemorrhagiae. The highest seroprevalences was found in males since it was the largest group in the canine population tested. These results suggest that the canine population may be a possible reservoir of leptospirosis in the studied area. It is necessary to conduct studies that permit the isolation and characterization of the serovars prevalent in this region