Suelo, agua y manejo

- Suelo y agua: Indicadores de calidad y degradación. Manejo: control de la salinidad, alcalinidad y drenaje | Margarita M Alconada Magliano. - Experiencias en el manejo del drenaje. Subsolado y Cincel | Cecilia Cerisola. - Drenes corrugados, PVC | Marisol Cuellas y Margarita Alconada M.Facultad de Ciencias Agrarias y Forestale

Molecular profiling of circulating tumor cells links plasticity to the metastatic process in endometrial cancer

Background About 20% of patients diagnosed with endometrial cancer (EC) are considered high-risk with unfavorable prognosis. In the framework of the European Network for Individualized Treatment in EC (ENITEC), we investigated the presence and phenotypic features of Circulating Tumor Cells (CTC) in high-risk EC patients. Methods CTC isolation was carried out in peripheral blood samples from 34 patients, ranging from Grade 3 Stage IB to Stage IV carcinomas and recurrences, and 27 healthy controls using two methodologies. Samples were subjected to EpCAM-based immunoisolation using the CELLection™ Epithelial Enrich kit (Invitrogen, Dynal) followed by RTqPCR analysis. The phenotypic determinants of endometrial CTC in terms of pathogenesis, hormone receptor pathways, stem cell markers and epithelial to mesenchymal transition (EMT) drivers were asked. Kruskal-Wallis analysis followed by Dunn’s post-test was used for comparisons between groups. Statistical significance was set at p < 0.05. Results EpCAM-based immunoisolation positively detected CTC in high-risk endometrial cancer patients. CTC characterization indicated a remarkable plasticity phenotype defined by the expression of the EMT markers ETV5, NOTCH1, SNAI1, TGFB1, ZEB1 and ZEB2. In addition, the expression of ALDH and CD44 pointed to an association with stemness, while the expression of CTNNB1, STS, GDF15, RELA, RUNX1, BRAF and PIK3CA suggested potential therapeutic targets. We further recapitulated the EMT phenotype found in endometrial CTC through the up-regulation of ETV5 in an EC cell line, and validated in an animal model of systemic dissemination the propensity of these CTC in the accomplishment of metastasis. Conclusions Our results associate the presence of CTC with high-risk EC. Gene-expression profiling characterized a CTC-plasticity phenotype with stemness and EMT features. We finally recapitulated this CTC-phenotype by over-expressing ETV5 in the EC cell line Hec1A and demonstrated an advantage in the promotion of metastasis in an in vivo mouse model of CTC dissemination and homing

Arsenic and fluoride in water in northwestern Buenos Aires : their association with natural landscape elements

Numerous publications analyze the origin of Arsenic and Fluoride, their behavior and distribution in groundwater indicating the importance in understanding the relation between their occurrence with other physical and chemical properties. Here, such properties associated with elements of the landscape in the plain of the Chaco-Pampean region are discussed. The landscape is represented by hills, lagoons and plains used for agricultural purposes. Water was sampled in mills, piezometers and lagoons following sections with different topographic elevation, soil type and vegetation in nine field campaigns from 2011 to 2013. Results in Lincoln are compared to localities in Trenque Lauquen, Pehuajó and Carlos Casares. Hydro geochemical modeling assisted to define composition and origin of the water from local mineralogy in soil and subsoil. Three major water groups were defined HCO3-Na, HCO3>Cl-Na and HCO3>Cl>SO4-Na. Piezometers were located at the top and base of hills with the presence of Acuic Hapludolls – Natric thapto Hapludolls, and Typic Natraqualfs with halophytic vegetation, respectively. Arsenic and Fluoride presence was not fully explained by groundwater chemistry type and association with HCO3-, Na+, salinity and pH, although generally were linked to alkaline soil and halophyte vegetation. High Arsenic and Fluoride presence in groundwater are explained by discharge flows travelling long distances through a thick sedimentary sequence; relative low concentration is refereed to local travelling flows recharged in nearby high terrain. Groundwater origin and its relations with other elements of the landscape permitted an understanding of groundwater functioning and to anticipate a general situation with respect Arsenic and Fluoride contents. In the present study this criterion is applied to analyze the relation of the occurrence of Arsenic (as total Arsenic, TAs) and Fluoride (F-) content in water of the Lincoln region in the Buenos Aires province of Argentina.Inst. de Clima y AguaFil: Alconada Magliano, Margarita María. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Edafología; ArgentinaFil: Damiano, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; ArgentinaFil: Carrillo-Rivera, José Joel Universidad Nacional Autónoma de México. Instituto de Geografía; MéxicoFil: Fagundo-Castillo, Juan Reynerio. Centro Nacional de Medicina Natural y Tradicional; Cub

Arsenic and fluoride in water in northwestern Buenos Aires: their association with natural landscape elements

Numerous publications analyze the origin of Arsenic and Fluoride, their behavior and distribution in groundwater indicating the importance in understanding the relation between their occurrence with other physical and chemical properties. Here, such properties associated with elements of the landscape in the plain of the Chaco-Pampean region are discussed. The landscape is represented by hills, lagoons and plains used for agricultural purposes. Water was sampled in mills, piezometers and lagoons following sections with different topographic elevation, soil type and vegetation in nine field campaigns from 2011 to 2013. Results in Lincoln are compared to localities in Trenque Lauquen, Pehuajó and Carlos Casares. Hydro geochemical modeling assisted to define composition and origin of the water from local mineralogy in soil and subsoil. Three major water groups were defined HCO3- Na, HCO3>Cl-Na and HCO3>Cl>SO4-Na. Piezometers were located at the top and base of hills with the presence of Acuic Hapludolls – Natric thapto Hapludolls, and Typic Natraqualfs with halophytic vegetation, respectively. Arsenic and Fluoride presence was not fully explained by groundwater chemistry type and association with HCO3 -, Na+, salinity and pH, although generally were linked to alkaline soil and halophyte vegetation. High Arsenic and Fluoride presence in groundwater are explained by discharge flows travelling long distances through a thick sedimentary sequence; relative low concentration is refereed to local travelling flows recharged in nearby high terrain. Groundwater origin and its relations with other elements of the landscape permitted an understanding of groundwater functioning and to anticipate a general situation with respect Arsenic and Fluoride contents. In the present study this criterion is applied to analyze the relation of the occurrence of Arsenic (as total Arsenic, TAs) and Fluoride (F-) content in water of the Lincoln region in the Buenos Aires province of Argentina.Facultad de Ciencias Agrarias y Forestale

Molecular profiling of circulating tumor cells links plasticity to the metastatic process in endometrial cancer

Background: About 20% of patients diagnosed with endometrial cancer (EC) are considered high-risk with unfavorable prognosis. In the framework of the European Network for Individualized Treatment in EC (ENITEC), we investigated the presence and phenotypic features of Circulating Tumor Cells (CTC) in high-risk EC patients. Methods: CTC isolation was carried out in peripheral blood samples from 34 patients, ranging from Grade 3 Stage IB to Stage IV carcinomas and recurrences, and 27 healthy controls using two methodologies. Samples were subjected to EpCAM-based immunoisolation using the CELLection™ Epithelial Enrich kit (Invitrogen, Dynal) followed by RTqPCR analysis. The phenotypic determinants of endometrial CTC in terms of pathogenesis, hormone receptor pathways, stem cell markers and epithelial to mesenchymal transition (EMT) drivers were asked. Kruskal-Wallis analysis followed by Dunn's post-test was used for comparisons between groups. Statistical significance was set at p < 0.05. Results: EpCAM-based immunoisolation positively detected CTC in high-risk endometrial cancer patients. CTC characterization indicated a remarkable plasticity phenotype defined by the expression of the EMT markers ETV5, NOTCH1, SNAI1, TGFB1, ZEB1 and ZEB2. In addition, the expression of ALDH and CD44 pointed to an association with stemness, while the expression of CTNNB1, STS, GDF15, RELA, RUNX1, BRAF and PIK3CA suggested potential therapeutic targets. We further recapitulated the EMT phenotype found in endometrial CTC through the up-regulation of ETV5 in an EC cell line, and validated in an animal model of systemic dissemination the propensity of these CTC in the accomplishment of metastasis. Conclusions: Our results associate the presence of CTC with high-risk EC. Gene-expression profiling characterized a CTC-plasticity phenotype with stemness and EMT features. We finally recapitulated this CTC-phenotype by over-expressing ETV5 in the EC cell line Hec1A and demonstrated an advantage in the promotion of metastasis in an in vivo mouse model of CTC dissemination and homing

Neurogenesis Is Reduced at 48 h in the Subventricular Zone Independent of Cell Death in a Piglet Model of Perinatal Hypoxia-Ischemia

Cellular and tissue damage triggered after hypoxia-ischemia (HI) can be generalized and affect the neurogenic niches present in the central nervous system. As neuroregeneration may be critical for optimizing functional recovery in neonatal encephalopathy, the goal of the present work was to investigate the neurogenic response to HI in the neurogenic niche of the subventricular zone (SVZ) in the neonatal piglet. A total of 13 large white male piglets aged <24 h were randomized into two groups: i) HI group (n = 7), animals submitted to transient cerebral HI and resuscitation; and ii) Control group (n = 6), non-HI animals. At 48 h, piglets were euthanized, and the SVZ and its surrounding regions, such as caudate and periventricular white matter, were analyzed for histology using hematoxylin-eosin staining and immunohistochemistry by evaluating the presence of cleaved caspase 3 and TUNEL positive cells, together with the cell proliferation/neurogenesis markers Ki67 (cell proliferation), GFAP (neural stem cells processes), Sox2 (neural stem/progenitor cells), and doublecortin (DCX, a marker of immature migrating neuroblasts). Hypoxic-ischemic piglets showed a decrease in cellularity in the SVZ independent of cell death, together with decreased length of neural stem cells processes, neuroblast chains area, DCX immunoreactivity, and lower number of Ki67 + and Ki67 + Sox2 + cells. These data suggest a reduction in both cell proliferation and neurogenesis in the SVZ of the neonatal piglet, which could in turn compromise the replacement of the lost neurons and the achievement of global repair.This work was supported by the United Kingdom Medical Research Council (G0501259), Basque Government Postdoctoral Program (POS_2013_1_191), EITB Maratoia- BIOEF (BIO18/IC/003), and the Spanish Ministry of Science and Innovation (MINECOR20/P66/AEI/10.13039/50110001103). This study was undertaken at University College London Hospitals/University College London, which received a proportion of funding from the UK Department of Health’s National Institute for Health Research Biomedical Research Centers funding scheme

Neurogenesis Is Reduced at 48 h in the Subventricular Zone Independent of Cell Death in a Piglet Model of Perinatal Hypoxia-Ischemia

Cellular and tissue damage triggered after hypoxia-ischemia (HI) can be generalized and affect the neurogenic niches present in the central nervous system. As neuroregeneration may be critical for optimizing functional recovery in neonatal encephalopathy, the goal of the present work was to investigate the neurogenic response to HI in the neurogenic niche of the subventricular zone (SVZ) in the neonatal piglet. A total of 13 large white male piglets aged <24 h were randomized into two groups: i) HI group (n = 7), animals submitted to transient cerebral HI and resuscitation; and ii) Control group (n = 6), non-HI animals. At 48 h, piglets were euthanized, and the SVZ and its surrounding regions, such as caudate and periventricular white matter, were analyzed for histology using hematoxylin-eosin staining and immunohistochemistry by evaluating the presence of cleaved caspase 3 and TUNEL positive cells, together with the cell proliferation/neurogenesis markers Ki67 (cell proliferation), GFAP (neural stem cells processes), Sox2 (neural stem/progenitor cells), and doublecortin (DCX, a marker of immature migrating neuroblasts). Hypoxic-ischemic piglets showed a decrease in cellularity in the SVZ independent of cell death, together with decreased length of neural stem cells processes, neuroblast chains area, DCX immunoreactivity, and lower number of Ki67 + and Ki67 + Sox2 + cells. These data suggest a reduction in both cell proliferation and neurogenesis in the SVZ of the neonatal piglet, which could in turn compromise the replacement of the lost neurons and the achievement of global repair