Microbial content of abattoir wastewater and its contaminated soil in Lagos, Nigeria

Microbial content of wastewater in two abattoirs and the impact on microbial population of receiving soil was studied in Agege and Ojo Local Government Areas in Lagos State, Nigeria. Wastewatersamples were collected from each of the abattoirs over three months period and examined for microbial content. Soil samples contaminated with the wastewaters were also collected and analysed formicrobial content as compared to soil without wastewater contamination in the neighbourhood (control). Some physico-chemical parameters of the samples such as total dissolved solid, chemical oxygen demand etc were examined. The wastewater samples from both abattoirs were highlycontaminated; Agege abattoir showed mean bacterial count of 3.32x107 cfu/ml and Odo abattoir showed mean count of 2.7x107 cfu/ml. The mean fungal populations were 1.6x 105 and 1.2x05 cfu/ml for Agege and Odo abattoirs respectively. In the contaminated soil sample, mean bacterial count was 3.36x107 cfu/ml compared to the 1.74x106 cfu/ml of the control sample. High microbial load in abattoir wastewater with negative effects on microbial population in soil, in this study, further confirmed the need to treat wastewater rather than discharging it to the environment

Geographical information system (GIS) application for planning and improvement of public water supply in Ota, Ogun State, Nigeria

Geographical information system (GIS) technique is a useful tool for spatial analysis which has not been fully exploited in solving water issues in Ota hence the study. The study applied the tool of GIS in the planning and improvement of water supply system in Ota, Ogun state. This study harnessed the synergy of GIS and engineering designs to proffer an acceptable solution to the problems of water supply in the study area. Primary data generated on the field with GPS and spatial information were analysed with the aid of GIS to produce contour pattern and Digital Elevation Model (DEM) in form of maps. The study noted that the population would increase to almost double the present population by the year 2040 invariably resulting to an increase in water demand of the population beyond the capacity of the existing water supply facilities. Results also showed that water demand by the projected population by year 2040 will increase to 68.37 million litres per day while the existing facility has the capacity to produce about 9.2 million litres per day. Maps produced by the study included Contour, Digital Elevation and Existing and proposed pipeline network maps. These maps made available more information which can then be used for improving the water supply system. The study proffered a lasting solution through design of water scheme with capacity large enough to cater for water requirement of the population for the next twenty-five years irrespective of variation in water need of individual residents in the area with designs to improvedistribution system through extension of pipelines and identification of various locations for service reservoirs.Keywords: GIS, design period, per capita

Albiglutide, a Long Lasting Glucagon-Like Peptide-1 Analog, Protects the Rat Heart against Ischemia/Reperfusion Injury: Evidence for Improving Cardiac Metabolic Efficiency

BACKGROUND: The cardioprotective effects of glucagon-like peptide-1 (GLP-1) and analogs have been previously reported. We tested the hypothesis that albiglutide, a novel long half-life analog of GLP-1, may protect the heart against I/R injury by increasing carbohydrate utilization and improving cardiac energetic efficiency. METHODS/PRINCIPAL FINDINGS: Sprague-Dawley rats were treated with albiglutide and subjected to 30 min myocardial ischemia followed by 24 h reperfusion. Left ventricle infarct size, hemodynamics, function and energetics were determined. In addition, cardiac glucose disposal, carbohydrate metabolism and metabolic gene expression were assessed. Albiglutide significantly reduced infarct size and concomitantly improved post-ischemic hemodynamics, cardiac function and energetic parameters. Albiglutide markedly increased both in vivo and ex vivo cardiac glucose uptake while reducing lactate efflux. Analysis of metabolic substrate utilization directly in the heart showed that albiglutide increased the relative carbohydrate versus fat oxidation which in part was due to an increase in both glucose and lactate oxidation. Metabolic gene expression analysis indicated upregulation of key glucose metabolism genes in the non-ischemic myocardium by albiglutide. CONCLUSION/SIGNIFICANCE: Albiglutide reduced myocardial infarct size and improved cardiac function and energetics following myocardial I/R injury. The observed benefits were associated with enhanced myocardial glucose uptake and a shift toward a more energetically favorable substrate metabolism by increasing both glucose and lactate oxidation. These findings suggest that albiglutide may have direct therapeutic potential for improving cardiac energetics and function

Laparoscopy in management of appendicitis in high-, middle-, and low-income countries: a multicenter, prospective, cohort study.

BACKGROUND: Appendicitis is the most common abdominal surgical emergency worldwide. Differences between high- and low-income settings in the availability of laparoscopic appendectomy, alternative management choices, and outcomes are poorly described. The aim was to identify variation in surgical management and outcomes of appendicitis within low-, middle-, and high-Human Development Index (HDI) countries worldwide. METHODS: This is a multicenter, international prospective cohort study. Consecutive sampling of patients undergoing emergency appendectomy over 6 months was conducted. Follow-up lasted 30 days. RESULTS: 4546 patients from 52 countries underwent appendectomy (2499 high-, 1540 middle-, and 507 low-HDI groups). Surgical site infection (SSI) rates were higher in low-HDI (OR 2.57, 95% CI 1.33-4.99, p = 0.005) but not middle-HDI countries (OR 1.38, 95% CI 0.76-2.52, p = 0.291), compared with high-HDI countries after adjustment. A laparoscopic approach was common in high-HDI countries (1693/2499, 67.7%), but infrequent in low-HDI (41/507, 8.1%) and middle-HDI (132/1540, 8.6%) groups. After accounting for case-mix, laparoscopy was still associated with fewer overall complications (OR 0.55, 95% CI 0.42-0.71, p < 0.001) and SSIs (OR 0.22, 95% CI 0.14-0.33, p < 0.001). In propensity-score matched groups within low-/middle-HDI countries, laparoscopy was still associated with fewer overall complications (OR 0.23 95% CI 0.11-0.44) and SSI (OR 0.21 95% CI 0.09-0.45). CONCLUSION: A laparoscopic approach is associated with better outcomes and availability appears to differ by country HDI. Despite the profound clinical, operational, and financial barriers to its widespread introduction, laparoscopy could significantly improve outcomes for patients in low-resource environments. TRIAL REGISTRATION: NCT02179112

Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy

Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe

Global variation in anastomosis and end colostomy formation following left-sided colorectal resection

Background End colostomy rates following colorectal resection vary across institutions in high-income settings, being influenced by patient, disease, surgeon and system factors. This study aimed to assess global variation in end colostomy rates after left-sided colorectal resection. Methods This study comprised an analysis of GlobalSurg-1 and -2 international, prospective, observational cohort studies (2014, 2016), including consecutive adult patients undergoing elective or emergency left-sided colorectal resection within discrete 2-week windows. Countries were grouped into high-, middle- and low-income tertiles according to the United Nations Human Development Index (HDI). Factors associated with colostomy formation versus primary anastomosis were explored using a multilevel, multivariable logistic regression model. Results In total, 1635 patients from 242 hospitals in 57 countries undergoing left-sided colorectal resection were included: 113 (6·9 per cent) from low-HDI, 254 (15·5 per cent) from middle-HDI and 1268 (77·6 per cent) from high-HDI countries. There was a higher proportion of patients with perforated disease (57·5, 40·9 and 35·4 per cent; P < 0·001) and subsequent use of end colostomy (52·2, 24·8 and 18·9 per cent; P < 0·001) in low- compared with middle- and high-HDI settings. The association with colostomy use in low-HDI settings persisted (odds ratio (OR) 3·20, 95 per cent c.i. 1·35 to 7·57; P = 0·008) after risk adjustment for malignant disease (OR 2·34, 1·65 to 3·32; P < 0·001), emergency surgery (OR 4·08, 2·73 to 6·10; P < 0·001), time to operation at least 48 h (OR 1·99, 1·28 to 3·09; P = 0·002) and disease perforation (OR 4·00, 2·81 to 5·69; P < 0·001). Conclusion Global differences existed in the proportion of patients receiving end stomas after left-sided colorectal resection based on income, which went beyond case mix alone

Economic assessment of tillage systems and weed control methods in maize (Zea mays L.) cultivation in southwestern Nigeria

This study was conducted to appraise the economics of different land preparation systems and weed management options in maize cultivation in three ecological zones (Ikenne, Ibadan and Ilorin) of southwestern Nigeria. Four tillage systems as main treatments and six weed control methods as sub-treatments were accommodated in a split-block arrangement of randomized complete block design with three replications. The main treatments were no-tillage (NT), ploughing and harrowing (PH), ploughing twice (PP), ploughing twice and harrowing (PPH) while the sub-treatments were atrazine plus metolachlor (Primextra) at 2.5 and 3.5 kg a.i. ha-1, atrazine plus pendimethalin at 1.25 + 0.83 and 1.75 + 1.16 kg a.i. ha-1, handweeded and unweeded checks. Net benefits achieved from no-tillage system were lower than that of mechanical tillage in all the locations. Greater net returns were obtained from PPH and PH treatments where handweeding or chemical weeding was used. Increased herbicide rate was not economical under the mechanical tillage systems. Any of the mechanical tillage methods can be employed in the three locations. Atrazine plus metolachlor or atrazine plus pendimethalin mixtures are profitable for maize production in Ikenne and Ibadan while the latter is profitable in Ilorin. Keywords: Tillage; weed control; economic; herbicides; maizeMoor Journal of Agricultural Research Vol. 7 (1&2) 2006 pp. 15-2

Effect Of Fermentation Containers On The Chemical Composition Of Fermented Sesame ( Sesamum Indicum L) Seeds

The importance of utilizing oilseeds as complementary nutrient sources for human consumption has received considerable attention in recent years. There exists wide varieties of oil crops including sesame seed ( Sesamum indicum L.), which are reported in literature to be rich in oil and nutrients; however, the presence of anti nutritional factors limits its uses. Processing grain using fermentation results in enhanced nutrition, stabilization of original raw materials, and detoxification of anti nutritional factors. There was lack of adequate traditional fermentation containers hence the need for use of some modern containers. This work, therefore, studied the effects of using banana leaf and plastic (high density polyethylene) bowl as containers for fermentation on the nutrients and anti nutritional factors of sesame seed. Samples were fermented separately using banana leaf and plastic bowl for seven days at temperature of 35 ± 2°C. Samples were drawn at intervals during fermentation to determine proximate composition, elemental concentrations and anti-nutritional factors concentrations using standard procedures. The pH decreased in the first 5 days and then increased as fermentation progressed coupled with a consistent rise in titratable acidity. Proximate analysis showed an increase in ash and crude fat contents with corresponding decrease in the carbohydrate and protein contents during fermentation. Protein ranged between 15.25% and 15.37% in banana leaf and plastic bowl respectively, compared to raw seed (26.20%). Fat increased from 51.02% in raw sesame to 60.20% and 59.33% in banana leaf and plastic bowl, respectively. However, fermented samples obtained from the plastic bowl had higher vitamin (thiamine and riboflavin) and minerals (calcium, phosphorus, potassium, magnesium, iron, selenium, zinc and manganese) in comparison to samples fermented in banana leaf. Fermentation in banana leaf and plastic bowl significantly reduced oxalate content of raw sesame by 35.40% and 29.12% respectively. In addition, phytate content was significantly reduced by 36.37% and 34.43% respectively. The present study showed that fermentation container had significant effect on nutritional composition during the fermentation of sesame seed

Agroclimatological Indices and Performance of Okra in Mixtures with Sorghum and Maize in Forest-Savanna Transition Zone of Nigeria.

Agroclimatological indices and performance of okra in mixtures with sorghum and maize in a Forest-Savanna transition zone of Nigeria was investigated at the Experimental Research Farmland of the National Horticultural Research Institutes (NIHORT), Ibadan during the 2009 and 2010 cropping seasons. Plants phenological stages formed the basic unit of time for the investigation. During these phenological stages, agroclimatological thermal and moisture indices were measured daily and processed into ten-day (decadal) averages likewise selected agronomic growth and yield parameters of the components crops were taken fortnightly. The results showed that the 2010 season crops had relatively longer growth duration, received more rainfall than 2009 season (692mm vs 487.2mm) while 2009 experienced warmer temperature during establishment and early vegetative stage than 2010 season (33.2°C vs 32°C), and (28.5°C vs 27 °C) during the reproductive phase for 2009 and 2010 season respectively. The mean pod yields of okra in both seasons were dependent on crop combination since pod yield in sorghum cultivars mixtures (Farin Dawa and Janare) (97.33 and 93.67 pods) was significantly higher than in maize mixtures (58.33 and 49.65 pods) in 2009 season likewise in 2010 season when okra pods in sorghum mixtures (Farin Dawa and Janare) had (309.67 and 232.33 pods) against (162.67 and 67 pods) in maize mixtures for the two sorghum cultivars. Result of correlation analysis showed that growing period rainfall contributes more to plant height and leaf area of the components crops than mean temperature. Key words: Phenological stages, agroclimatological indices, okra, sorghum, maiz

Comparative Catalytic Properties of Ni(OH)2 and NiO Nanoparticles Towards the Degradation of Nitrite (NO2 - ) and Nitric Oxide (NO)

Nitrite (NO2 - ) and nitric oxide (NO) have been identified as an environmentally hazardous analytes from discharged industrial effluents. Thus in this study, nickel oxide (NiO) and nickel hydroxide (Ni(OH)2) nanoparticles were synthesized using the complexation-precipitation method and their catalytic properties towards NO2 - and NO investigated. The success of the synthesised nanoparticles was confirmed using characterisation techniques, such as X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and the Fourier transformed infrared (FTIR) spectroscopy. The sizes of the synthesized NiO and Ni(OH)2 nanoparticles were estimated to be 5.39 and 5.07 nm respectively. The catalytic behaviour of NiO and Ni(OH)2 nanoparticles towards the oxidative degradation of NO and NO2 - in acidic and neutral media respectively was studied using UV-Vis spectrophotometer. Result indicated that NiO nanoparticles demonstrated better catalytic properties at different reaction time towards NO2 - and NO oxidation compared to Ni(OH)2, while NiO and Ni(OH)2 at nano scale showed enhanced catalysis towards the analytes compared with the bulk Ni salt. The bulk Ni salt did not show any sensing properties towards NO2 - . However in NO, the absorbance intensity due to the generation of nitrate (NO2 - ) was five times higher in the presence of NiO nanoparticles compared with the bulk Ni salt. The improved catalysis of Ni(OH)2 and NiO nanoparticles in this study was attributed to effective pore sizes and large surface area which expose the analytes to more catalytic site. The nanoparticles are simple to prepare, therefore can be used for the fabrication of a simple, portable, miniaturized nitrite and nitric oxide nanosensor for potential clinical and analytical application