A method for Cloud Mapping in the Field of View of the Infra-Red Camera during the EUSO-SPB1 flight

EUSO-SPB1 was released on April 24th, 2017, from the NASA balloon launch site in Wanaka (New Zealand) and landed on the South Pacific Ocean on May 7th. The data collected by the instruments onboard the balloon were analyzed to search UV pulse signatures of UHECR (Ultra High Energy Cosmic Rays) air showers. Indirect measurements of UHECRs can be affected by cloud presence during nighttime, therefore it is crucial to know the meteorological conditions during the observation period of the detector. During the flight, the onboard EUSO-SPB1 UCIRC camera (University of Chicago Infra-Red Camera), acquired images in the field of view of the UV telescope. The available nighttime and daytime images include information on meteorological conditions of the atmosphere observed in two infra-red bands. The presence of clouds has been investigated employing a method developed to provide a dense cloudiness map for each available infra-red image. The final masks are intended to give pixel cloudiness information at the IR-camera pixel resolution that is nearly 4-times higher than the one of the UV-camera. In this work, cloudiness maps are obtained by using an expert system based on the analysis of different low-level image features. Furthermore, an image enhancement step was needed to be applied as a preprocessing step to deal with uncalibrated data

La flora vascolare del Lazio.

The vascular flora of Lazio (Central Italy) - The long and meticulous data raising on the vascular flora of Lazio region, started by Giuseppe Lusina, then carried on by Bruno Anzalone and finally inherited by Edda Lattanzi and Mauro Iberite, represents a first working phase lasted 60 years and is briefly introduced in this paper. The vascular flora of Lazio region currently consists of 3330 units and 3146 species, divided into 150 families and 896 genera. For each unit the following information is provided: synonym(s), commonness in the regional territory, IUCN threatening status, geographical areas in which it occurs, ideal altitude range and habitat. Since the publication of the latest regional checklist (1996-98), the total amount of units increased from 3185 to 3330 (+4.46%); this is mainly due to the substantial and lively contribution of floristic and taxonomic studies provided both by researchers and by amateurs. Due to the complex geological and environmental patch as well as to the geographical position of Lazio region, this flora is to be considered one of the richest in Italy. Finally, a summarizing outline is given of the priority vascular plants of the region: 1) very rare units, 2) possibly extinct units, 3) threatened units, 4) units uniquely occurring in Lazio region, 5) non-native units; an outline that can serve as an up-to-date scientific tool in managing the floristic heritage of Lazio region

Cloud distribution evaluated by the WRF model during the EUSO-SPB1 flight

EUSO-SPB1 was a balloon-borne mission of the JEM-EUSO (Joint Experiment Missions for Ex-treme Universe Space Observatory) Program aiming at the ultra-high energy cosmic ray (UHECR) observationsfrom space. We operated the EUSO-SPB1 telescope consisting of 1 m2Fresnel refractive optics and multi-anode photomultiplier tubes. With a total of 2304 channels, each performed the photon counting every 2.5 s,allowing for spatiotemporal imaging of the air shower events in an 11◦ 11◦field of view. EUSO-SPB1was the first balloon-borne fluorescence detector with a potential to detect air shower events initiated by theEeV energy cosmic rays. On 24 April 2017 UTC, EUSO-SPB1 was launched on the NASA’s Super PressureBalloon that flew at 16 – 33 km flight height for 12 days. Before the flight was terminated, 27 hours ofdata acquired in the air shower detection mode were transmitted to the ground. In the present work, we aim atevaluating the role of the clouds during the operation of EUSO-SPB1. We employ the WRF (Weather Researchand Forecasting) model to numerically simulate the cloud distribution below EUSO-SPB1. We discuss the keyresults of the WRF model and the impact of the clouds on the air shower measurement and the efficiency of thecosmic ray observation. The present work is a part of the collaborative effort to estimate the exposure for airshower detections

Mediastinal syndrome from plasmablastic lymphoma in human immunodeficiency virus and human herpes virus 8 negative patient with polycythemia vera: a case report

Background: Plasmoblastic lymphoma is a rare and aggressive subtype of diffuse large B cell lymphoma, which occurs usually in the jaw of immunocompromised subjects. Case presentation: We describe the occurrence of plasmoblastic lymphoma in the mediastinum and chest wall skin of an human immunodeficiency virus-negative 63-year-old Caucasian man who had had polycytemia vera 7 years before. At admission, the patient showed a superior vena cava syndrome, with persistent dyspnoea, cough, and distension of the jugular veins. Imaging findings showed a 9.7 × 8 × 5.7 cm mediastinal mass. A chest wall neoformation biopsy and ultrasound-guided fine-needle aspiration biopsy of the mediastinal mass allowed diagnosis of plasmoblastic lymphoma and establishment of an immediate chemotherapeutic regimen, with rapid remission of compression symptoms. Conclusions: Plasmoblastic lymphoma is a very uncommon, difficult to diagnose, and aggressive disease. The presented case represents the first rare mediastinal plasmoblastic lymphoma in a human immunodeficiency virus-/human herpesvirus-8-negative patient. Pathologists should be aware that this tumor does appear in sites other than the oral cavity. Fine-needle aspiration biopsy is a low-cost, repeatable, easy-to-perform technique, with a high diagnostic accuracy and with very low complication and mortality rates. Fine-needle aspiration biopsy could represent the right alternative to surgery in those patients affected by plasmoblastic lymphoma, being rapid and minimally invasive. It allowed establishment of prompt medical treatment with subsequent considerable reduction of the neoplastic tissue and resolution of the mediastinal syndrome

Estimation of the exposure for the air shower detection mode of EUSO-SPB1

EUSO-SPB1 was a balloon-borne pathfinder mission of the JEM-EUSO (Joint Experiment Missions for the Extreme Universe Space Observatory) program. A 12-day long flight started from New Zealand on April 25th, 2017 on-board the NASA's Super Pressure Balloon. With capability of detecting EeV energy air showers, the data acquisition was performed using a 1 m^2 two-Fresnel-lens UV-sensitive telescope with fast readout electronics in the air shower detection mode over ~30 hours at ~16--30 km above South Pacific. Using a variety of approaches, we searched for air shower events. Up to now, no air shower events have been identified. The effective exposure, regarding the role of the clouds in particular, was estimated based on the air shower and detector simulations together with a numerical weather forecast model. Compared with the case assuming the fully clear atmosphere conditions, more than ~60% of showers are detectable regardless the presence of the clouds. The studies in the present work will be applied in the follow-up pathfinders and in the future full-scale missions in the JEM-EUSO program

Projected Quasi-particle Perturbation theory

The BCS and/or HFB theories are extended by treating the effect of four quasi-particle states perturbatively. The approach is tested on the pairing hamiltonian, showing that it combines the advantage of standard perturbation theory valid at low pairing strength and of non-perturbative approaches breaking particle number valid at higher pairing strength. Including the restoration of particle number, further improves the description of pairing correlation. In the presented test, the agreement between the exact solution and the combined perturbative + projection is almost perfect. The proposed method scales friendly when the number of particles increases and provides a simple alternative to other more complicated approaches

Isotope correlations as a probe for freeze-out characterization: central 124Sn+64Ni, 112Sn+58Ni collisions

124Sn+64Ni and 112Sn+58Ni reactions at 35 AMeV incident energy were studied with the forward part of CHIMERA multi-detector. The most central collisions were selected by means of a multidimensional analysis. The characteristics of the source formed in the central collisions, as size, temperature and volume, were inspected. The measured isotopes of light fragments (3 <= Z <=8) were used to examine isotope yield ratios that provide information on the free neutron to proton densities.Comment: 4 pages, Contribution to 8th International Conference on Nucleus-Nucleus Collisions, Moscow 200

Mass and charge identification of fragments detected with the Chimera Silicon-CsI(Tl) telescopes

Mass and charge identification of charged products detected with Silicon-CsI(Tl) telescopes of the Chimera apparatus is presented. An identification function, based on the Bethe-Bloch formula, is used to fit empirical correlation between Delta E and E ADC readings, in order to determine, event by event, the atomic and mass numbers of the detected charged reaction products prior to energy calibration.Comment: 24 pages, 7 .jpg figures, submitted to Nucl.Instr.

Novel in vitro and in vivo data on the cellular localization of Hsp10 in smokers affected by COPD and in lung-derived cell lines exposed to cigarette smoke extract as stressor

Cigarette smoke is a potent stressor for the respiratory system, contributing to pathogenesis, for instance in chronic obstructive pulmonary disease (COPD), but its effects on the expression, function, and cellular localization of mitochondrial chaperonins are still largely unknown. We studied in vivo (airways biopsies) the localization of Hsp10 and Hsp60 in patients (smokers and non-smokers) affected by mild-moderate COPD, and characterized the effects of non-lethal doses of cigarette smoke extract (CSE) on the expression of these molecules in two human cell lines: lung fibroblasts (HFL-1) and bronchial epithelial (16HBE). We applied various in vitro methods: immunohistochemistry (IHC), subcellular fractionation analyses (SFA), Western blotting (WB), immunocytochemistry (ICC), and transmission electron microscopy (TEM) immunogold, and used bioinformatics and databases searches to gather structural in silico data for interpreting and complementing the in vitro results. IHC showed that in smokers and non-smokers COPD patients Hsp10 was localized in both, the cytoplasm and the nucleus of epithelial and lamina propria cells, while Hsp60 was present only in the cytosol. ICC, SFA, and WB on both CSE-exposed cell lines confirmed the presence of nuclear Hsp10, with an increasing trend in parallel to CSE concentration. TEM immunogold further confirmed Hsp10 in the nucleus, in addition to its presence in the cytoplasm and mitochondria, on both cell lines. Bioinformatics and in silico structural analyses indicated that Hsp10 can localize in extramitochondrial sites, such as the nucleus, even if Hsp10 lacks known DNA-binding motifs or nuclear import signals in its primary sequence. Our data suggest a link between exposure to exogenous oxidative stress and cell response, involving Hsp10, which would play roles different from its canonical functions. It is known that Hsp10 can display an array of functions depending on its location: cytoplasm, mitochondria, or extracellular. Here, we show for the first time the presence of Hsp10 in the nucleus of epithelial and stromal human-lung cell lines, paralleling the observations in vivo in COPD patients, and indicating that intranuclear Hsp10 levels are affected by oxidative stress due to an exogenous stressor like cigarette-smoke. The questions now are by what mechanism Hsp10 becomes a resident of the nucleus and what are its functions there.

Nuclear localization and new isoforms detection give new insights on Hsp10 functions in normal and cigarette smoke-stressed lung cells

Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular dis- tribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Cigarette smoke (CS) is a potent stressor for the respiratory system, but its effects on the expression, function, and cellular locali- zation of mitochondrial chaperonins are still largely unknown. We studied in vivo (airways biopsies) the localization of Hsp10 and Hsp60 in patients (smokers and non-smokers) affected by mild-moderate COPD, and charac- terized the effects of non-lethal doses of CS extract (CSE) on the expression of these molecules in two human cell lines: lung fibroblasts (HFL-1) and bronchial epithelial cells (16HBE). We applied various in vitro methods: IHC, subcellular fractionation analyses (SFA), western blotting (WB), ICC, transmission electron microscopy (TEM) immunogold, chromati protein extracts (CPE), as well as 2D-gel based proteomics analyses. Bioinformatics was used to gather structural in silico data. IHC showed that Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers. ICC, SFA, and WB showed that 16HBE and HFL-1 cells featured nuclear Hsp10, before and after CSE exposure; TEM immunogold further confirmed this observation. Proteomics data showed that CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts. Bioinformatics analyses indicated that Hsp10 can localize in extramitochondrial sites, such as the nucleus, even if Hsp10 lacks known DNA-binding motifs or nuclear import signals. Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein as suggested by CPE/gel retardation experiments. Data reported here indicate that in human cells of the respiratory mucosa there are at least three different intracellular locales for Hsp10: mitochondrial, nuclear, and cyto- solic. Further experiments are en route for the definition of the mechanisms underlying the transfer of Hsp10 to the nucleus and other cellular/extracellular compartments. This work was supported by grants from University of Palermo (FFR 2012) to GLR