Preparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome c

PMID = 3042377

and photophysical investigations

A highly selective and sensitive fluorescent sensor for mercury ion has been obtained from a combination of Bodipy and Calix[4]arene derived by click chemistry. The structural characterization of all compounds had been carried out by FT-IR, H-1 NMR, C-13 NMR, MS, elemental analysis or melting point. Calix[4]-B including a calix[4]arene and two Bodipy units is a good fluorescent sensor for Hg(II). It can be explained by the coordination between mercury and the triazole rings. In a co-solvent of methanol and water, the complexation ratio for Calix[4]-B/Hg(II) was determined as 1:1 and the binding constant was found to be 3.42 x 10 (7) M-1. The detection of Hg(II) by Calix[4]-B works selectively in aqueous methanol mixture. (C) 2017 Elsevier B.V. All rights reserved

parameters

The aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze-thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10 mM raffinose, 5 mM hypotaurine, 5 mM raffinose +2.5 mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 degrees C for 25 s in a water bath for evaluation.The addition of raffinose led to higher percentages of subjective and CASA motilities (47.5 +/- 12.2%, 46.3 +/- 13.6%) compared to controls (38.8 +/- 13.8%, 30.5 +/- 11.7%, P < 0.05). For the CASA progressive motility, 5 mM raffinose (20.12 +/- 8.82%) had increasing effect in comparison to control (10 +/- 7.94%, P <0.05) following the freeze-thawing process. Raffinose and hypotaurine led to higher viability (40.8 +/- 4.68%, 40.8 +/- 4.7%), high sperm mitochondrial activity (29.5 +/- 5.4%, 27.3 +/- 4.9%) and acrosome integrity (50.8 +/- 8.1, 50.7 +/- 4.4) percentages, compared to control groups (31.5 +/- 3.5%, 9.5 +/- 8.2%, 42.8 +/- 7.3%, P < 0.05). H + R group only led to high sperm mitochondrial activity when compared to control group. In the comet test, raffinose and hypotaurine resulted in lower sperm with damaged DNA (6.2% and 3.9%) than that of control (9.1%), reducing the DNA damage. For TUNEL assay, The TUNEL-positive cell was distinguished by distinct nuclear staining. Raffinose and H + R groups resulted in lower sperm with TUNEL-positive cell (1.5 +/- 1.2% and 2.1 +/- 0.9%) than that of control (4.9 +/- 2.5%) (P < 0.05). In conclusion, findings of this study showed that raffinose and hypotaurine supplementation in semen extenders provided a better protection of sperm parameters against cryopreservation injury, in comparison to the control groups. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved

Sorption of heavy metal ions by glass beads-immobilized calix[4]arenes derivative

Glass beads (GB) immobilized, 5,11,17,23-tetra-tert-butyl-25,27-diethoxycarbonylmethoxy-26,28-dihydroxycalix[4]arene (CA) are prepared and used as a new sorbent in sorption study of removal heavy metal ions. A calixarene derivative bonded to amino-functionalized glass beads sorbent was synthesized via a self assembly technique for sorbent of selected heavy metal ions in aqueous. In order to absorb selected heavy metal ions in aqueous, a calixarene derivative bonded to amino-functionalized glass beads sorbent was synthesized via a self assembly technique. The sorbent which is named GB-APTS-CA was characterized using infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), elemental analysis and thermal analysis (TGA/DTG). The influences of some experimental parameters including pH of the sample solution, weight of sorbent, concentration and temperature have been investigated. The sorption data were evaluated using the Langmuir, Freundlich and Dubinin Radushkevich (D-R) isotherm. The obtained maximum sorption capacity for Cu(II), and Pb(II) is 0.06 mmol g−1 and 0.02 mmol g−1, respectively. Thermodynamic parameters such as the standard free energy change (ΔG○), enthalpy change (ΔH○) and entropy change (ΔS○) were calculated to determine the nature of sorption process. Thus, GB-APTS-CA is favorable and useful for the removal of Cu(II) and Pb(II) metal ions

Naked-eye fluorescent sensor for Cu(II) based on indole conjugate BODIPY dye

A novel on/off fluorescent indole/BODIPY-based Cu2+ chemosensor (3) was synthesized by Knoevenagel condensation of BODIPY derivative 1 with 2-methyl-indole-3-carbaldehyde 2. The identity of compound 3 was confirmed by H-1 NMR, C-13 NMR, FT-IR, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and single crystal X-ray diffraction techniques. Fluorescent chemosensor (3) is found to be highly selective and sensitive for detection of Cu2+ with a color change from purple to yellow. The optical sensor features for the Cu2+ complex of 3 were investigated by UV-Vis and fluorescence spectroscopy. The addition of Cu2+ caused quenching of fluorescence intensity and the detection limit was calculated to be 0.124 mu M. Also, the stoichiometry ratio of 3+Cu2+ was obtained 2:1 by Job's plot. Live cell image, flow cytometry and cytotoxic properties of compound 3 were examined. (C) 2016 Elsevier Ltd. All rights reserved