Attitudes of venture capital investors towards entrepreneurs with previous business failure, Belfast (June)

Business failure represents a significant outcome of entrepreneurial activity and yet remains an underdeveloped area of research. This article focuses on the attitudes of VC investors towards entrepreneurs with a previous failure experience. It illustrates that VCs recognise the complex, contextual nature of failure and do not necessarily perceive the entrepreneur to be the primary cause of the venture's demise. Consequently, the article differentiates between 'business', 'entrepreneurial' and 'venture capitalist' failure. The article demonstrates that VCs often adopt a tolerant, flexible and open-minded attitude to failure and are keen to understand the circumstances in which it occurred. The majority of the VCs in the study emphasise that their decision to invest in an entrepreneur is not negatively affected to any significant degree by a previous experience of failure. A number of influential factors are presented, such as a high quality concept, which can offset this aspect of the entrepreneur's track record. The article concludes that business failure is not automatically considered a 'black mark' by VCs. It is important for entrepreneurs involved in business failure to be aware of these positive and sympathetic attitudes when considering putting forward new proposals to the VC community

Management Strategies Of Non-Profit Community Sport Facilities In An Era Of Austerity

Research Question: This qualitative research explores the impact of austerity on community sport facilities across England (United Kingdom), drawing upon resource dependence theory (RDT) embedded within network theory. Research Methods: In-depth semi-structured interview data were collected from 24 stakeholders related to community sport facilities (n=12 facility managers, n=6 regional grant managers, n=6 national funders both third sector and corporate). The qualitative data were thematically analysed to understand the impact of austerity on how community sport facilities managed their organisations and operations. Results and Findings: The findings from this research offer insight into the challenges that community sport facilities are encountering which have resulted from austerity, and a shrinking of the funding from central Government to local public services. Furthermore, different community sport facilities have navigated these challenges to maintain sustainability, essentially through adapting network structure and through income dynamism. In addition, using a network theory approach alongside RDT within a sporting context, has allowed us to address issues on how network flow and structure impact sustainability and operations within and between organisations. Implications: The article offers managerial recommendations for community sport facility managers, practitioners and policy makers who operate in times of fiscal constraint. It recommends that future sport research utilises and applies both RDT and network theory to examine these changes and the subsequent management strategies adopted to overcome the associated challenges of fiscal constraint

Analysis of Affymetrix GeneChip Data Using Amplified RNA

The standard method of target synthesis for hybridization to Affymetrix GeneChip® expression microarrays requires a relatively large amount of input total RNA (1-15 micrograms). When small biological samples are collected by microdissection or other methods, amplification techniques are required to provide sufficient target for hybridization to expression arrays. One amplification technique used is to perform two successive rounds of T7-based in vitro transcription. However, the use of random primers required to re-generate cDNA from the first round transcription reaction results in shortened copies of the cDNA, and ultimately the cRNA, transcripts from which the 5\u27 end is missing. In this paper we describe an experiment designed to compare the quality of data obtained from labeling small RNA samples using the Affymetrix Small Sample Target Labeling Protocol V 2 to that of data obtained using the standard protocol. We utilized different preprocessing algorithms to compare the data generated using both labeling methods and present a new algorithm that improves upon existing ones in this setting

An analytical and experimental assessment of flexible road ironwork support structures

This paper describes work undertaken to investigate the mechanical performance of road ironwork installations in highways, concentrating on the chamber construction. The principal aim was to provide the background research which would allow improved designs to be developed to reduce the incidence of failures through improvements to the structural continuity between the installation and the surrounding pavement. In doing this, recycled polymeric construction materials (Jig Brix) were studied with a view to including them in future designs and specifications. This paper concentrates on the Finite Element (FE) analysis of traditional (masonry) and flexible road ironwork structures incorporating Jig Brix. The global and local buckling capacity of the Jig Brix elements was investigated and results compared well with laboratory measurements. FE models have also been developed for full-scale traditional (masonry) and flexible installations in a surrounding flexible (asphalt) pavement structure. Predictions of response to wheel loading were compared with full-scale laboratory measurements. Good agreement was achieved with the traditional (masonry) construction but poorer agreement for the flexible construction. Predictions from the FE model indicated that the use of flexible elements significantly reduces the tensile horizontal strain on the surface of the surrounding asphaltic material which is likely to reduce the incidence of surface cracking

Evaluating the integrity of forested riparian buffers over a large area using LiDAR data and Google Earth Engine

Spatial and temporal changes in the land cover affect water quality in the streams and other water bodies. Stream riparian areas are increasingly relevant as the human modification of the landscape continue unabated. The objectives of this study were to: (1) determine the classes and the distribution of land cover in stream riparian areas; (2) examine the accuracy of the existing land cover data National Land Cover Database (NLCD) using high-resolution imagery NAIIP and LiDAR data; and (3) evaluate the integrity of forested riparian buffers areas in the Lower Savannah River basin. The land cover map was produced using a Support Vector Machine (SVM) algorithm supervised classification through the cloud-based Google Earth Engine platform with an overall accuracy assessment of 83.66%. LiDAR data analysis were implemented using ArcGIS 10.6. The result of this study demonstrates that LiDAR data can be used to accurately map the vegetation width, height and canopy cover within the riparian buffer over wide areas to support ecological-based management. It is also highlighted that the open-access imagery and the efficient geospatial analysis GEE provides a reliable methodology to remotely monitor forest cover and land use in the riparian buffer areas

Enhanced immunogenicity of an HIV-1 DNA vaccine delivered with electroporation via combined intramuscular and intradermal routes

It is accepted that an effective prophylactic HIV-1 vaccine is likely to have the greatest impact on viral transmission rates. As previous reports have implicated DNA-priming, protein boost regimens to be efficient activators of humoral responses, we sought to optimize this regimen to further augment vaccine immunogenicity. Here we evaluated single versus concurrent intradermal (i.d.) and intramuscular (i.m.) vaccinations as a DNA-priming strategy for their abilities to elicit humoral and cellular responses against a model HIV-1 vaccine antigen, CN54-gp140. To further augment vaccine-elicited T and B cell responses, we enhanced cellular transfection with electroporation and then boosted the DNA-primed responses with homologous protein delivered subcutaneously (s.c.), intranasally (i.n.), i.m., or transcutaneously (t.c.). In mice, the concurrent priming regimen resulted in significantly elevated gamma interferon T cell responses and high-avidity antigen-specific IgG B cell responses, a hallmark of B cell maturation. Protein boosting of the concurrent DNA strategy further enhanced IgG concentrations but had little impact on T cell reactivity. Interestingly protein boosting by the subcutaneous route increased antibody avidity to a greater extent than protein boosting by either the i.m., i.n., or t.c. route, suggesting that this route may be preferential for driving B cell maturation. Using an alternative and larger animal model, the rabbit, we found the concurrent DNA-priming strategy followed by s.c. protein boosting to again be capable of eliciting high-avidity humoral responses and to also be able to neutralize HIV-1 pseudoviruses from diverse clades (clades A, B, and C). Taken together, we show that concurrent multiple-route DNA vaccinations induce strong cellular immunity, in addition to potent and high-avidity humoral immune responses. IMPORTANCE The route of vaccination has profound effects on prevailing immune responses. Due to the insufficient immunogenicity and protection of current DNA delivery strategies, we evaluated concurrent DNA delivery via simultaneous administration of plasmid DNA by the i.m. and i.d. routes. The rationale behind this study was to provide clear evidence of the utility of concurrent vaccinations for an upcoming human clinical trial. Furthermore, this work will guide future preclinical studies by evaluating the use of model antigens and plasmids for prime-boost strategies. This paper will be of interest not only to virologists and vaccinologists working in the HIV field but also to researchers working in other viral vaccine settings and, critically, to the wider field of vaccine delivery

Comparative efficacy of long-acting bronchodilators for COPD - a network meta-analysis

Background: Clinicians are faced with an increasingly difficult choice regarding the optimal bronchodilator for patients with chronic obstructive pulmonary disease (COPD) given the number of new treatments. The objective of this study is to evaluate the comparative efficacy of indacaterol 75/150/300 μg once daily (OD), glycopyrronium bromide 50 μg OD, tiotropium bromide 18 μg/5 μg OD, salmeterol 50 μg twice daily (BID), formoterol 12 μg BID, and placebo for moderate to severe COPD. Methods: Forty randomized controlled trials were combined in a Bayesian network meta-analysis. Outcomes of interest were trough and post-dose forced expiratory volume in 1 second (FEV1), St. George’s Respiratory Questionnaire (SGRQ) score and responders (≥4 points), and Transition Dyspnea Index (TDI) score and responders (≥1 point) at 6 months. Results: Indacaterol was associated with a higher trough FEV1 than other active treatments (difference for indacaterol 150 μg and 300 μg versus placebo: 152 mL (95% credible interval (CrI): 126, 179); 160 mL (95% CrI: 133, 187)) and the greatest improvement in SGRQ score (difference for indacaterol 150 μg and 300 μg versus placebo: -3.9 (95% CrI -5.2, -2.6); -3.6 (95% CrI -4.8, -2.3)). Glycopyrronium and tiotropium 18 μg resulted in the next best estimates for both outcomes with minor differences (difference for glycopyrronium versus tiotropium for trough FEV1 and SGRQ: 18 mL (95% CrI: -16, 51); -0.55 (95% CrI: -2.04, 0.92). Conclusion: In terms of trough FEV1 and SGRQ score indacaterol, glycopyrronium, and tiotropium are expected to be the most effective bronchodilators

Roll-out of SARS-CoV-2 testing for healthcare workers at a large NHS foundation trust in the United Kingdom, March 2020

Healthcare workers (HCW) are potentially at increased risk of infection with coronavirus disease (COVID-19) and may transmit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to vulnerable patients. We present results from staff testing at Sheffield Teaching Hospitals NHS Foundation Trust, United Kingdom. Between 16 and 29 March 2020, 1,533 symptomatic HCW were tested, of whom 282 (18%) were positive for SARS-CoV-2. Testing HCW is a crucial strategy to optimise staffing levels during this outbreak