Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex

Epithelial monolayers are one-cell thick tissue sheets that separate internal and external environments. As part of their function, they have to withstand extrinsic mechanical stresses applied at high strain rates. However, little is known about how monolayers respond to mechanical deformations. Here, by subjecting suspended epithelial monolayers to stretch, we find that they dissipate stresses on a minute time-scale in a process that involves an increase in monolayer length, pointing to active remodelling of cell architecture during relaxation. Strikingly, monolayers consisting of tens of thousands of cells relax stress with similar dynamics to single rounded cells and both respond similarly to perturbations of actomyosin. By contrast, cell-cell junctional complexes and intermediate filaments do not relax tissue stress, but form stable connections between cells, allowing monolayers to behave rheologically as single cells. Taken together our data show that actomyosin dynamics governs the rheological properties of epithelial monolayers, dissipating applied stresses, and enabling changes in monolayer length.Peer ReviewedPostprint (published version

Analysis of Tyrosine Kinase Inhibitor-Mediated Decline in Contractile Force in Rat Engineered Heart Tissue

Introduction Left ventricular dysfunction is a frequent and potentially severe side effect of many tyrosine kinase inhibitors (TKI). The mode of toxicity is not identified, but may include impairment of mitochondrial or sarcomeric function, autophagy or angiogenesis, either as an on-target or off-target mechanism. Methods and Results We studied concentration-response curves and time courses for nine TKIs in three-dimensional, force generating engineered heart tissue (EHT) from neonatal rat heart cells. We detected a concentration- and time-dependent decline in contractile force for gefitinib, lapatinib, sunitinib, imatinib, sorafenib, vandetanib and lestaurtinib and no decline in contractile force for erlotinib and dasatinib after 96 hours of incubation. The decline in contractile force was associated with an impairment of autophagy (LC3 Western blot) and appearance of autophagolysosomes (transmission electron microscopy). Conclusion This study demonstrates the feasibility to study TKI-mediated force effects in EHTs and identifies an association between a decline in contractility and inhibition of autophagic flux

Evolution of millimeter-wave communications toward next generation in wireless technologies

Next generation in wireless communication systems being deployed in the world, 5G/6G mobile and wireless communication technologies has been widely studied. This work clarifies that Millimeter-Wave (mm-Wave) is in its early stages and will be driven by consumers who keep on desire higher information rates for the consumption of media. Millimeter-Wave innovation represents for next generation cellular technology and includes a wide range of advanced features which make next innovation most dominant technology in near future, these abilities incorporate high achievable information rates in addition to lower delays and constant connectivity on wireless devices

Performance analysis of IEEE 802.11ac based WLAN in wireless communication systems

IEEE 802.11ac based wireless local area network (WLAN) is emerging WiFi standard at 5 GHz, it is new gigabit-per-second standard providing premium services. IEEE 802.11ac accomplishes its crude speed increment by pushing on three distinct measurements firstly is more channel holding, expanded from a maximum of 80 MHz up to 160 MHz modes. Secondly, the denser modulation, now using 256-QAM, it has the ability to increase the data rates up to 7 Gbps using an 8×8 multiple input multiple output (MIMO). Finally, it provides high resolution for both narrow and medium bandwidth channels. This work presents a study to improve the performance of IEEE 802.11ac based WLAN system

Performance improvement of fractional N-PLL synthesizers for digital communication applications

Loop filter with two order was designed to improve the performance of the fractional N-phase locked loop (PLL) circuit (reference spurs noise and switching time), decreasing these two factors give good characteristic to fractional N-PLL circuit, the second order and third order loop filters are widely used in frequency synthesizer because they give good stability tolerance and for their simple architecture. They are designed at bandwidth B=125 KHz and its multipoles, at two values of the phase margin (pm)= 35°, 57°. MATLAB program was used to find the lock time, the component values for each element in the loop filter, also the filter impedance T(s), the bode plot of frequency response for close loop (CL) and open loop gain (OL). It is found by comparing the result of the frequency response for the 2nd order loop filter and 3rd order loop filter, that increasing the order of the filter will reduce the spurs noise that destroy the received signal at receiving side

Clinical presentation of Parkinson's disease among Sudanese patients

Parkinson Disease (PD) is a neurodegenerative disorder affecting motor system. It is a chronic progressive disorder leading to long standing disability.Objective: To study the clinical presentation of PD among Sudanese patients seen at Elshaab Teaching Hospital during the period from May2004-April 2008.Methodology: In this descriptive prospective, cross sectional hospital based study, 94 patients were studied using standardized questionnaire including history and clinical examination.Result: The total number diagnosed to have PD was 94 patients. Male to female ratio was found to be1.5:1.Common age group affected was 70-80 years (24.47%).The common presenting symptom was found to be poverty of movement (93.6%) followed by tremor (82.98%). On neurologicalexamination; rigidity, dyskinesia and festinate gait were the common signs. Primitive reflexes were found in significant number of patients. Idiopathic PD was found to be the common type (75.53%). Of the side effects of benzhexol, 66.67% of our patients developed dry mouth. Postural hypotension was seen in 10.42% of the patients who were taking levodopa.Conclusion: The clinical presentations of our patients does not differ from what was mentioned in the literatureKeywords: rigidity, dyskinesia, festinate gait, benzhexol, levodopa.

Slac2-b Coordinates Extracellular Vesicle Secretion to Regulate Keratinocyte Adhesion and Migration

Slac2-b, also known as exophilin-5, is a Rab27b effector protein with a role in exosome transport and is encoded by the EXPH5 gene. We previously described biallelic loss-of-function mutations in EXPH5 in an autosomal recessive form of epidermolysis bullosa simplex. However, how the loss of Slac2-b expression leads to skin fragility and erosions is unknown. In this study, we demonstrate that keratinocytes (KCs) isolated from two different individuals with mutations in EXPH5 have significant defects in cell‒matrix adhesion. EXPH5-mutant KCs also showed increased perinuclear accumulation and significantly reduced trafficking of CD63+ vesicles. These phenotypes were also seen in Slac2-b‒deficient KCs. This was coincident with a reduction in Rab27a protein expression in Slac2-b‒mutant KCs as well as reduced secretion of extracellular vesicles containing extracellular matrix proteins. Live imaging analysis revealed a strong correlation between CD63+ vesicle trafficking to the plasma membrane and focal adhesion dynamics. These findings support a role for Slac2-b in regulating local focal adhesion dynamics to support effective KC adhesion and provide insight into the underlying pathophysiology of inherited skin blistering.</p

An investigation study for risk calculation of security vulnerabilities on android applications

Applications within mobile devices, although useful and entertaining, come with security risks to private information stored within the device such as name, address, and date of birth. Standards, frameworks, models, and metrics have been proposed and implemented to combat these security vulnerabilities, but they remain to persist today. In this review, we discuss the risk calculation of android applications which is used to determine the overall security of an application. Besides, we also present and discuss the permission-based access control models that can be used to evaluate application access to user data. The study also focuses on examining the predictive analysis of security risks using machine learning. We conduct a comprehensive review of the leading studies accomplished on investigating the vulnerabilities of the applications for the Android mobile platform. The review examines various well-known vulnerabilities prediction models and highlights the sources of the vulnerabilities, prediction technique, applications and the performance of these models. Some models and frameworks prove to be promising but there is still much more research needed to be done regarding security for Android applications

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac