Experimental study on reperfusion of intraocular lens

作者简介: 祁明信, 男, 1945 年7 月 出生, 教授、主任医师、博士研究生 导师, 主要从事白内障的基础与临 床研究。联系电话: 0591-83570887; E-mail:qihuang@netease. com 通讯作者: 黄秀榕,E-mail:[email protected][中文文摘]目的开展晶状体再灌注的离体和动物实验研究,并对再灌注人工晶状体技术进行评价。方法采用新鲜离体幼兔眼、离体猪眼、新西兰白兔眼,应用自行研制的人工晶状体材料,进行以下实验:(1)体外固化实验;(2)晶状体前囊膜微型撕囊及其稳固性实验;(3)经微型前囊膜开口超声乳化吸出晶状体内容物实验;(4)活的新西兰白兔眼内人工晶状体再灌注实验。结果(1)按硅酮聚合物与固化剂50:1的比例可获得柔软、弹性好、固化时间短(完全固化时间为60min)的注入材料;(2)晶状体前囊膜1.8～2.0mm的连续环形撕囊口具有较好的稳定性,可经该微型开口吸出晶状体内容物并灌注材料;(3)超声能量18%、流量25mL·min-1、负压120mmHg(1kPa=7.5mmHg)为晶状体内容物经微型前囊膜开口吸出的最佳条件;(4)注入灌注材料后可形成由晶状体囊膜包裹的、置换原晶状体皮质和核的、新的再灌注人工晶状体。结论采用再灌注人工晶状体的方法可进行新型人工晶状体再灌注,可为治疗白内障和老视提供参考。[英文文摘]Objective To carry out the experimental study on reperfusion of intraocular lens(IOLs) in vitro or in animal,and to assess the technique of IOLs reperfusion.Methods The following experiments were performed by using self-developed materials in fresh rabbit eyes and pig eyes in vitro,as well as in eyes of alive New-Zea-land rabbits:(1)Solidification study of self-developed material in vitro;(2)Continuous circular capsulorhexis(CCC) in anterior capsule of lens and its stability;(3)Draw of lens contents via phaco through mini-CCC;(4)IOLs ref illing in the eyes of alive New-Zea land rabbits. Results(1) Thematerialwhich was soft, springy and short-term solidification(full solidification time was 60 minutes) were obtained in certain proportion of geland solidified agent(50:1) in vitro; ( 2)The CCC in anterior capsule of lens with 1.8-2.0 mm diameter had very good stability. The lens contents were drawn and the materialwere refilled through themini-CCC; (3) The best conditions of drawing out lens contents through m ini-CCC were phaco energy 18% , flow 25 mL·min- 1, and negative pressure 120 mmH g (1kPa=7.5 mmHg);(4) The new refilled IOLs, which were wrapped by capsule of lens and were replaced original cortex and nucleus of lens, were obtained after thematerial refillied. Conc lusion. New IOLs are refilled through this method, which can prov ide reference for the treatment of cataract and presbyopia.福建省科技三项费用;教育厅重点资助项目基金资助(编号:K98041

高速受电弓气动减阻的仿真优化研究

随着绿色环保的现代轨道交通设计理念发展，受电弓减阻成为制约高速列车提速的关键问题之一。高速列车运行时，受电弓暴露于流线型车体之外，是列车气动阻力的主要来源之一，随着列车速度的提高，受电弓的减阻问题亟待解决。本文针对某型高速受电弓，基于计算流体动力学仿真技术，分析了整弓气动阻力分布，确定滑板与底座的压差阻力是气动阻力的主要来源，提出了滑板流线型减阻外壳与底座包裹流线型减阻外壳的优化方案，并与原模型对比验证了减阻效果。计算表明，减阻模型在350 km/h运行时，整弓气动阻力在开口、闭口时分别降低25.13%与24.19%

基于模态实验的单滑板受电弓全柔模型修正方法

动车组通过受电弓从接触网上获取电能,良好的弓网接触是列车受流的重要保障.随着列车速度的提高,弓网动态特性问题日益突出.受电弓在高速或更高速运行时,接触网不平顺、气动效应等高频激励,将激发受电弓的高频弹性模态及富有高频成分的弓网相互作用力.只考虑受电弓3个垂向自由度的三质量块模型不再适用于高频弓网动力学分析,为进行更高速下的受电弓动力学参数设计和弓网受流质量评估,需建立反映结构弹性模态的受电弓全柔模型.文章提出基于模态实验的受电弓全柔模型的修正方法.首先,开展一款新型单滑板高速受电弓的模态实验,获得260 Hz以内的两阶垂向耦合振动模态参数和6阶垂向弹性模态参数.然后,进行受电弓模态频率对材料参数的灵敏度分析,研究得到弓头、上臂和下臂的弹性模量和密度及弓头弹簧刚度,对受电弓的8阶垂向模态频率的影响显著,确定了模型修正的参数.最后,利用粒子群优化算法,获得与模态实验结果吻合度较高的修正全柔模型,其与实验结果的误差仅为5.2%.此外,提出基于模态置信度的振型识别方法,实现了迭代寻优过程中正确率为100%的模态自动识别

Probing Target and Designing Nicotinoid Derivatives for Antitumor Leading Compound MED

通过变性荧光素酶的再复性实验发现真菌环氧二烯(MyCOEPOXydIEnE,MEd)对热休克蛋白(HEAT SHOCk PrOTEIn 90,HSP90)具有抑制作用.WESTErn blOT实验结果表明,MEd影响人宫颈癌细胞株HElA中HSP70及HSP90的客户蛋白质(AkT,rAf-1)的表达,表明MEd是HSP90的抑制剂.通过靶向对接技术预测了MEd与人HSP90 n端ATP结合位点的结合情况,并在此基础上发现,MEd的烟酸类衍生物4-ndM与HSP90的结合具有比MEd与HSP90更强的亲和作用.体外实验结果证明,MEd的烟酸类衍生物4-ndM及3-ndM对HElA细胞表现出比MEd更强的细胞毒活性;可通过上调HSP70,并下调AkT和rAf-1而影响HElA细胞中HSP90相关蛋白质的表达.由此推测,MEd及其烟酸类衍生物可以通过抑制HSP90,而使其客户蛋白AkT或rAf-1发生降解,发挥其抗肿瘤作用.Antitumor lead compound mycoepoxydiene(MED) was isolated from marine fungus in the year of 2002,but till now the mechanism of MED against cancer is still unclearly.Heat shock protein 90(Hsp90) has emerged in recent years as a promising new target for anticancer therapies,owing to a lot of tumorigenesis relative client proteins need the help of Hsp90 for folding and keeping stable.The experiment of denatural lucife-rase renature showed that MED is a potential inhibitor of Hsp90,which stimuli us to perform the research for MED both as a novel inhibitor of Hsp90 and the antitumor leading compound.The nicotinoid derivatives of MED(NDM) was designed based on the structure of MED and the prediction of molecular docking program.MTT assay showed that nicotinoid derivate 4-NDM exhibited antitumor activity against HeLa cells in vitro with IC50 4.7 μmol/L.The derivate 4-NDM also can induce deregulation of the client protein Akt,Raf-1 and P-Akt,when treated HeLa cell with the compound,just as MED done.Compare with MED,4-NDM not only showed stronger antitumor activity,but also exhibited higher affinity for Hsp90.The results illustrate that MED and its nicotinoid derivatives may be target for Hsp90 as the inhibitor has antitumor activity.国家自然科学基金(批准号:30873148;30973566);国家“八六三”计划项目(批准号:2007AA091503)资

2002–2010年中国典型生态系统辐射及光能利用效率数据集

辐射是陆地生态系统能量的主要来源,其利用效率表现为光能利用率,反映了生态系统转化光能、生成有机物质的能力。揭示典型生态系统的辐射及光能利用效率可以为评估区域光能资源及其利用效率提供参考,也为评估区域有机物质固定能力及碳吸收能力提供依据。基于中国陆地生态系统通量观测研究联盟(China FLUX)的长期观测结果及已发表文献的公开数据,构建了2002–2010年中国典型生态系统辐射及光能利用效率数据集,包含51个生态系统126个站点年辐射、光能利用效率及吸收光能利用效率的观测记录。另外,本数据集还包含生态系统代码、年份、经度、纬度、海拔、生态系统类型、年均气温、年总降水量、年均CO2质量浓度、年均叶面积指数、最大叶面积指数等生物气候信息。本数据集可以为评估生态系统生产能力、应对气候变化等方面的研究提供数据支持

大连极紫外相干光源

先进光源的发展在前沿科学研究中发挥的作用越来越重要。近十年来,飞速发展的自由电子激光技术为科学家们提供了探索未知世界、发现新科学规律和实现技术变革的重要工具。建成的大连极紫外(EUV)相干光源的运行波段为50~150nm,单脉冲能量大于100μJ,且可提供10-12 s和10-13 s量级的超快激光脉冲,是我国第一台自由电子激光用户装置,并且是国际上唯一运行在极紫外波段的自由电子激光用户装置,在世界范围内为用户提供具有高峰值亮度和超短脉冲的极紫外激光。大连EUV相干光源是由国家自然科学基金委资助、由中国科学院大连化学物理研究所和上海应用物理研究所共同承担的重大科学仪器研制项目,目标是打造一个以先进极紫外光源为核心、主要用于能源基础科学研究的光子科学平台

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel