Secondary Metabolites of Aspergillus fumigatus var. fumigatus

目的:研究西双版纳粗榧共生真菌Aspergillus fumigatusvar .fumigatus的次生代谢产物。方法:通过柱层析法分离发酵产物的化学成分,并利用波谱数据和文献数据对照鉴定化合物的结构。结果:分离到2个化合物,分别鉴定为trypacidin (1)及其衍生物1 ,2-seco-trypacidin (2)。结论:化合物2是新结构;并对文献中关于trypacidin的C-4和C-7a位碳谱数据的指定进行了更正。AIM: To study the secondary metabolites of the commensal microorganism (Aspergillus fumigatusvar. fumigatus) of C ephalotaxus mannii. METHOD: Chemical constituents were isolated by column chromatography and structures were elucidated based on spectral analysis. RESULT: Two compounds were purified from the fermented extracts and i dentified to be trypacidin (1) and 1,2-seco-trypacidin ( 2), respectively. CONCLUSION: Compound 2 is a new compound, and the 13C NMR assignments for the C-4 and C- 7a of trypacidin were revised based on HMQC and HMBC experiments.国家杰出青年科学基金(No.30325044)资助项目~

双歧杆菌的遗传学研究

双歧杆菌的遗传学研究厦门大学生物系厦门361005杨智源黄耀坚苏文金1前言双歧杆菌（bIfIdObACTErIuM）于1899年由巴斯德研究所的TISSIEr从母乳婴儿粪便中发现，是定植于人类或某些哺乳动物体内的革兰氏阳性生理细菌，在微生态学上...福建省自然科学基金课题资

虫草属真菌经植物环节“宿主跳跃”的初步研究

从酿酒葡萄(Vitis vinifera L.)和番荔枝(Annona squamosa L.)中分离并鉴定两株植物内生来源的虫草属真菌和一株拟青霉,并就已知昆虫、块菌来源的虫草属真菌ITS分子序列建立了在不同宿主体内的虫草属真菌系统发育树,重现了其物种在协同进化历程中在不同宿主体内发育的现象.发现虫草属真菌在进化过程中除了昆虫与块菌外可能还经由植物的"宿主跳跃"环节

Studies on Arbuscular Mycorrhiza infection to Mangrove Plants in Fujian

2010年5月和12月,对福建沿海3个红树林生长区(洛阳江、九龙江口、漳江口)的红树林植物丛枝菌根(AM)侵染状况进行研究。结果表明:(1)红树林生长区中6种植物根内均发现AMf侵染结构,其中桐花树、秋茄、鱼藤和芦苇的丛枝为AruM(疆南星)型;(2)6种植物的丛枝菌根侵染率差异较大,老鼠簕的侵染率最高,鱼藤最低;(3)桐花树和秋茄的丛枝菌根侵染率呈显著差异,而其在不同生长区之间无差异;(4)桐花树和秋茄的丛枝菌根侵染率在不同时间呈显著差异,而钝草的丛枝菌根侵染率在不同时间的差异不显著。To study the symbiosis between arbuscular mycorrhizal fungi(AMF) and mangrove plant species,102 root and soil samples from 6 plant species were collected in three main mangroves growth zone in Fujian,in May and December of 2010.The main results showed that:(1)AMF infection structures were mostly found in the form of curled and expanded intracellular hyphae,vesicles,which were commonly associated with all the mangrove species we investigated.'Arum-type' arbuscules were found in Parmentiera cerifera,Kandelia obovata,Derris trifoliata and Phragmites australis;(2)AM infection rate was different in mangrove plant species,with Acanthus ilicifolius the highest and Derris trifoliate the lowest;(3) There were significant differences of AM infection rate existing between Parmentiera cerifera and Kandelia obovata,but no difference among three sampling sites;(4) There were also significant differences of AM infection rate in different period in Parmentiera cerifera and Kandelia obovata,but no significant difference in Stenotaphrum helferi in different period.厦门市科技项目(3502Z20072010、3502Z20102004);厦门市园林植物园园长基金(BG201101)资

STUDIES ON THE CHEMICAL CONSTITUENT FROM SEA CUCUMBER MENSAMARIA INTERCEDENS──Ⅱ.PMI-1, A NOVEL FUCOSAN

利用乙醇沉淀分级技术从二色桌片参双酶水解液里分离到一种纯的活性多糖，定名PMI-1。用高效薄层层析法鉴定其组成的单糖，发现PMI-1只含有L-岩藻糖。根据其分析结果和理化特性，PMI－1应是由若藻糖基和岩藻糖-4硫酸酯基组成的均多糖。研究PMI－1的核磁共振谱，可认定它是一种具有直链的和α1－α联接的均多糖。因此它是一种新的岩藻聚糖。Using double enzymes digestion and fractionations by precipitation with alcohol, a pure active polysaccharide PMI-1 was obtained from the dried body wall of Mensamaria intercedens. Monosaccharide composition was identified by thin-layer chromatography, the results indicate that PMI-1 only consisits of L-fucose. According to analytical and chemicophysical data, PMI-1 seemed to be homosaccharide consisted of fucose and fllcose-4sulfate with the molar ratio of 1: 1. Studied on the 1 HNMR and 13MNMR data of PMI-1 showed that PMI-1 had a straightsaccharide chain and α1-α linkage.福建省重中之重项

STUDY ON THE ANTIFUNGAL ACTIVITIES OF ENDOPHYTIC FUNGI ISOLATED FROM SEVERAL PHARMACEUTICAL PLANTS

从三尖杉 ,南方红豆杉及香榧中分离出 1 72株内生真菌 ,对其进行抗菌活性检测 ,结果表明共 90株内生真菌对一种或多种植物病原真菌 ,如红色面孢霉 (Neurosporasp .) ,木霉 (Trichodermasp .) ,镰刀菌 (Fusariumsp .)等有抑制作用 ,来自三尖杉、南方红豆杉和香榧的抗菌活性菌株比例分别为 40 %,5 4 2 %及 5 7 1 %。其中平板抑菌圈直径大于1 5mm的高抗菌株有 3 5株。按Ainsworth等鉴定系统和方法进行鉴定 ,具有抗菌活性的内生真菌主要分布于拟青霉属、镰孢菌属等 1 8个属中。教育部高等学校博士学科点专项科研基金资助项目 (No .96 0 384 0 2

Medium Optimization for Antitumor Agent Mycoepoxydiene by Marine Lignicolous Fungi Diaporthe sp.

从海洋木栖真菌HLY-2分离得到的真菌环氧二烯(Mycoepoxydiene)是新发现的高抗肿瘤活性化合物。通过正交设计,改良普通半海水PD培养基的配方,比较固体和液体两种发酵方法,得到了使该化合物产量大幅提高的最佳培养条件:在固体发酵条件下土豆250g/L,海水300mL/L,葡萄糖30g/L,乳糖50g/L,磷酸二氢钾0·65mmol/L,硫酸铵1g/L,产量高达543mg/L,比普通的PD培养基提高了43倍,也比液体发酵的最高产量提高了近15倍。同时还对固体发酵和液体发酵造成的产量差异进行了分析。并检验了发酵产物的抗肿瘤活性。Mycoepoxydiene is a novel antitumor agent extracted from marine lignicolous fungi HLY-2, which is Diaporthe phaseolorum by molecule identification. The medium optimization for mycoepoxydiene by orthogonal design and the comparison of submerged fermentation and solid state fermentation were studied. The rusult is that the maximal yield of the compound is 543mg/L, which is 43 times compared to the customary half-seawater PD medium and 15 times to the best submerged condition. This optimum culture medium included potato 250g/L, seawater 300mL/L, glucose 30g/L, lactose 50g/L, KH_ 2 PO_ 4 0.65mmol/L and (NH_ 4 )_ 2 SO_ 4 1g/L in the solid state condition. Differentiation analysis between submerged and solid state fermentation, and antitumor activity of these ferment products were also studied. The antitumor activity of products of the optimum medium approached the pure compound.国家自然科学基金资助项目(No.2003AA624010);; 福建省科技项目基金(No.2004N003);; 福建省自然科学基金(No.C0210001

A TWO-PARTICLE TURBIDIMETRIC LATEX IMMUNOASSAY FOR THE DETECTION OF SPECIFIC ANTIBODIES OF TRICHINELLA SPIRALIS

目的　建立旋毛虫病的快速检测技术。方法　基因工程抗原包被有色乳胶颗粒，抗抗体包被磁性颗粒，在抗体存在下形成抗原－ 乳胶－ 抗体－ 抗抗体－ 磁性颗粒的复合场，在磁场作用下沉淀下来，从而达到快速检测旋毛虫相关抗体的目的。结果　１９ 份人工感染鼠血清、５ 份人工感染猪血清、３ 份旋毛虫病猪血清、４ 份病人血清均呈阳性反应，而对照血清均为阴性反应；该方法在鼠感染旋毛虫后第五天可测出ＩｇＭ 抗体，第九天可测出ＩｇＧ抗体。结论　本检测技术简便易行，不需专用设备，可望成为一种快速诊断旋毛虫病的有效方法。Aim To establish a rapidly detecting method for the specific antibodies of Trichinella spiralis Method Latex was coated with p49/GST and polystyrene beads(Dynabeads)were coated with anti-antibodies SAM-IgG、SAH-IgG、RAP-IgG or SAM-IgM Then the test serum was incubated with two particles for 30 min at room temperature with slowly shaking After sedimentation of the polystyrene beads with a magnet,the turbidity of the resultant latex suspension was estimated with eyes or measured spectrophotometrically at a wavelength of 400nm Results Positive result of IgG antibody were found in 19 sera of experimental infected mice,3 sera of experimental infected swine and 4 sera of patients The IgM and IgG can be check up in mice after 5 and 9 days after experimental infection The result agreed with those obtained by ELISA Conclusion A two-particle turbidimetric latex immunoassy established by this study is a rapid,easy and precise method for the diagnosis of the trichinosis福建省科委(农医)项目!(95-Z150

产胞外布雷菲德菌素A青霉发酵条件的研究

通过正交设计试验,优化了青霉(Penicillium)属真菌SHZK-15产胞外布雷菲德菌素(B refeld in-A,简称BFA)的液体发酵条件。最佳的BFA发酵条件为:培养基含马铃薯200g(煮汁),葡萄糖20g,(NH4)2SO44.0g,KH2PO41.0g,MgSO4.7H2O 2.0g,CaCO35.0g,pH自然,瓶装量为100mL/250 mL,培养温度为28℃,转速为120 r/m in,培养时间为7d,BFA的最高产量可达151.6mg/L