Properties and Activity Regulation of N-Acetyl-β-D-glucosaminidase from Prawn Cuticle (Litopenaeus vannamei)

凡纳滨对虾(Litopenaeusvannamei)是全世界三大养殖对虾之一，具有重要的经济价值。N-乙酰-β-D-氨基葡萄糖苷酶(N-Acetyl-β-D-glucosaminidase或NAGase，EC.3.2.1.52)在对虾的幼体孵化，蜕壳发育，营养代谢，免疫防御中发挥着重要作用。探讨壳膜中NAGase的酶学特性、活力调控、催化作用机理并与内脏中NAGase性质与功能进行比较，能更全面地了解凡纳滨对虾不同组织来源的NAGase特点及作用。 以凡纳滨对虾壳膜为材料，经过0.01mol/LTris-HCl(pH7.5)缓冲液匀浆抽提处理，硫酸铵分级盐析纯化，获得粗酶。粗酶再经过Seph...Litopenaeus vannamei is one of the three most popular cultured prawns in the world,and has important economic value. N-Acetyl-β-D-glucosaminidase (NAGase, EC3.2.1.52) plays the important role in molting, nutrition assimilation and immunological defence of Litopenaeus vannamei. So, exploring NAGase’s enzymatic properties, activity control, catalyze mechanism from cuticle will beneficial to comprehe...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：2172008115266

养殖常用消毒药物对凡纳滨对虾NAGase活力的影响

研究几种消毒药物对来源于凡纳滨对虾壳膜与内脏的N-乙酰-β-D-氨基葡萄糖苷酶(NAGase,EC3.2.1.52)活力的影响。结果表明,次氯酸钠和三氯异氰尿酸对2种来源的NAGase活性均有较强的不可逆抑制作用;双链季铵盐对壳膜NAGase活性起强的激活作用,而对内脏NAGase有轻微的抑制作用;戊二醛对壳膜NAGase没有明显作用,对内脏NAGase具有轻微的激活作用

Developments and Applications of Paper-based Microfluidics

纸芯片微流控技术是一种新型微流控技术。相比于以玻璃、石英、高聚物等为基底的传统微流控芯片,纸芯片具有成本低、易操作、可携带、耗样量小等优点。该文介绍了纸芯片的发展及常用的制作方法,并举例说明了光度法、荧光法、化学发光及电化学发光法和电化学法在纸芯片检测中的应用;归纳了纸芯片技术在临床诊断、环境监控以及食品安全分析等方面的应用;最后对纸芯片微流控的应用前景进行了展望。Paper-based microfluidics stand out as a new class of microfluidic technology,and present distinguishing features such as low cost,ease of use,portability,and low reagent consumption compared with the conventional microfluidic devices.In this paper,the development of μPADs was first introduced,and the common fabrication techniques were presented.Then the methods for quantitative analysis on μPADs were summarized including colorimetry,fluorescence,chemiluminescence,electrochemiluminescence and electrochemistry with their applications in clinical diagnostics,environmental monitoring as well as food quality control.Finally,the potential and future outlooks ofμPADs were discussed.国家重点基础研究发展计划项目(2010CB732402;2013CB933703); 国家自然科学基金项目(91313302;21205100;21275122); 国家杰出青年科学基金项目(21325522

患红体病凡纳滨对虾肝胰腺NAGase基本性质的改变研究

[目的]为虾养殖过程中红体病的诊断和控制提供重要的理论依据。[方法]分别测定健康和患红体病的凡纳滨对虾(Litopenaeus vanname)的肝胰腺N-乙酰-β-D-氨基葡萄糖苷酶(EC3.2.1.52,NAGase)的比活力及其性质差异。[结果]2种来源NAGase的比活力、基本酶学性质等均存在差异。对虾患红体病后,肝胰腺NAGase的比活力和最适温度下降,但催化反应动力学常数Km、Vm值和活化能上升。[结论]该研究表明NAGase与对虾患病可能存在着密切关系

四苯基铁卟啉双氧加合物的密度泛函理论研究

采用密度泛函理论方法计算了自旋多重度为1、3、5的四苯基铁卟啉双氧加合物(FeTPPO2)的几何构型及电子性质。考察了四苯基铁卟啉对分子氧的活化。研究发现,FeTPPO2能量随自旋多重度升高而降低。五重态FeTPPO2的能量最低,但其铁卟啉对分子氧的活化效果最差。FeTPPO2中Oa—Ob键键长随自旋多重度变化的规律为三重态>单重态>五重态,说明三重态FeTPPO2中分子氧活化最明显。Fe—Oa键键长和Oa—Ob键键长呈负相关关系。电荷布居分析表明,FeTPPO2中分子氧活化程度与分子氧上所带负电荷多少有关,所带负电荷越多,分子氧活化越明显。分析自旋密度得知,三重态FeTPPO2中分子氧为单线态分子氧。三重态和五重态FeTPPO2中,铁离子均向分子氧转移了β电子,有利于铁氧键的形成

Ti-48Al-2Nb-2Cr合金铸造收缩特性研究

目的研究Ti Al合金铸造过程的收缩特性。方法采用Ti4822合金,通过设计长方体、圆柱体、回字形和环形4种典型结构,开展了熔模精密铸造工艺实验。结果基础尺寸在10~80 mm时,Ti4822合金的自由线收缩率在3.11%~2.09%之间,受阻收缩比自由收缩低30%~50%左右,同时随着基础尺寸的增加,自由线收缩量增加,而收缩率呈降低趋势。结论收缩数据和规律的获得,为Ti Al合金铸件尺寸精度控制提供了基础数据

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel