凡纳滨对虾(Litopenaeusvannamei)是全世界三大养殖对虾之一，具有重要的经济价值。N-乙酰-β-D-氨基葡萄糖苷酶(N-Acetyl-β-D-glucosaminidase或NAGase，EC.3.2.1.52)在对虾的幼体孵化，蜕壳发育，营养代谢，免疫防御中发挥着重要作用。探讨壳膜中NAGase的酶学特性、活力调控、催化作用机理并与内脏中NAGase性质与功能进行比较，能更全面地了解凡纳滨对虾不同组织来源的NAGase特点及作用。 以凡纳滨对虾壳膜为材料，经过0.01mol/LTris-HCl(pH7.5)缓冲液匀浆抽提处理，硫酸铵分级盐析纯化，获得粗酶。粗酶再经过Seph...Litopenaeus vannamei is one of the three most popular cultured prawns in the world,and has important economic value. N-Acetyl-β-D-glucosaminidase (NAGase, EC3.2.1.52) plays the important role in molting, nutrition assimilation and immunological defence of Litopenaeus vannamei. So, exploring NAGase’s enzymatic properties, activity control, catalyze mechanism from cuticle will beneficial to comprehe...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：2172008115266
