Quantitative Detection of Genetically Modified Insecticidal Maize by ELISA

应用纯化的Bt1杀虫晶体蛋白质作为标准蛋白和免疫抗原 ,通过抗体 抗原 酶标抗体反应 ,建立了间接酶联免疫吸附测定法 (ELISA) ,以快速检测转基因抗虫玉米中的Bt1表达蛋白 .用建立的ELISA法对 4种进口玉米实物样品进行了测定 ,实验结果得到了免疫印迹分析的验证 ,并与进口试剂盒方法的定量分析结果相一致Based on the reaction among antibody antigen enzyme antibody, using purified Bt1 insecticide crystal protein as standard protein and immunity antigen, a quantitative indirect enzyme linked immunosorbent assay (ELISA) method was established to detect Bt1 protein expressed in genetically modified maize.4 import maize samples were detected by the ELISA, compared to western blotting & KIT assay methods. and results were agreeable.厦门市科技计划资助项目 (35 0 2Z2 0 0 110 9

Further Discussions on Various Problems Concerning "the State of Xindu was Styled the State of Guangchuan in the Second Year of Emperor Jing"——Doubled as a consultation with Mr. Wang Wentao

对班固与颜师古所处时代及立场加以综合分析,并通过对“广川国“沿革的准确爬梳,得出《汉书.地理志》中关于“景帝二年为广川国“的论断无疑是正确的,而后半句“宣帝甘露三年复故“实应为“甘露四年“。A comprehensive analysis is made of the times and standpoints of Ban Gu and Yan Shigu.Through the exact clarifications of the origin of the state of Guangchuan,it is concluded that the statement of "being styled the state of Guangchuan in the second year of Emperor Jing"is unquestionably correct,whereas the latter half of the sentence "being styled its original name in the third year of Emperor Xuan" should have been "in the fourth year of Emperor Xuan"

AERODYNAMIC MOMENTS CONTROL OF WING MODEL USING PLASMA JET

为考察火花放电等离子体射流控制机翼气动力矩的效果,在NACA0021平直机翼模型上安装火花放电等离子体射流发生器,通过改变射流发生器安装位置、射流角度及加载电参数,研究其控制机翼模型气动力矩的性能及机理。在NACA0021机翼模型近前缘处,布置2个火花放电等离子体射流发生器,采用气动力测量技术,在来流风速为20 m/s时测得,攻角-4°~10°时,滚转力矩系数最大减小了0.0024,攻角为12°~16°时,滚转力矩系数最大增加了0.0021;偏航力矩系数最大减小了0.00097。实验研究结果表明:等离子体射流可改变机翼模型横航向气动力矩,并可通过改变射流角度和加载电压频率调节等离子体射流控制横向气动力矩的效果。To investigate the control effect of Spark Discharge Plasma Jets(SPJs) on the aerodynamic moments of a wing, SPJ generators were used for active flow control experimental study on an NACA0021 straight wing model. The location of SPJ generators along the chord of the airfoil, the jet flow direction relative to the chord, and the driving voltage parameters were changed to research the control effect and mechanism of SPJ generators on the aerodynamic moments of a wing model. The aerodynamic moments were measured with a six-component balance at a wind speed of 20 m/s. Two SPJ generators, arranged near the leading edge, reduced the rolling moment coefficient by a maximum of 0.0024 for angles of attack-4°~10°, but increased the rolling moment coefficient by a maximum of 0.0021 for angles of attack 12°~16°. The yaw moment coefficient was reduced by a maximum of 0.00097. The results show that aerodynamic moments control of wings can be realized using SPJs. The control effect of SPJs on the aerodynamic moments is changeable by adjusting the driving voltage frequency and the jet flow direction relative to the chord.航空科学基金项目(20141368007);; 福建省自然科学基金项目(2010J01014

旋毛虫病的免疫诊断研究

旋毛虫病的免疫诊断研究刘光明蔡海松综述宋思扬苏文金审校厦门大学生物学系（厦门361005）旋毛虫病的临床表现错综复杂，临床诊断较为困难，易与其它传染病相混淆。肌肉活检发现幼虫或囊包虽可确诊，但在轻度感染和感染早期往往不易检出，即使是感染晚期，因受摘取..

PURIFICATION of P49 ANTIGEN GENE PRODUCT of TRICHINELLA SPIRALLS EXPRESSED IN ESCHERICHIA COLI

目的建立旋毛虫P49抗原基因原校表达产物的纯化方法。方法菌体经冻融、超声破菌及提取包涵体后，用离子交换和凝胶过滤层析纯化蛋白质。结果纯化后的融合蛋白P49/gST经SdS-PAgE电冰鉴定达电泳纯。双抗体夹心ElISA法检测结果表明纯化的P49/gST融合蛋白能与旋毛虫感染的鼠血清和纯化融合蛋白免疫的兔血清反应，而不与正常民和兔血清反应。结论纯化后的融合蛋白P49/gST具有较高的纯度及较强的免疫活性，可望作为旅毛虫病的免疫诊断抗原。Aim To establish a purification procedure for p49 antigen gene product of Trichinella spiralis expressed inEschedchia coli.Methods After centrifugation of the bacterial culture, the bacterial pellet was resuspended and lysed byfreezing-thawing treatment and ultrasonication.Ion exchanged and gel filtration chromatography were used to purify the fu-sion protein p49/GST from the inclusion bodies- Re8ultS The purity of the fusion protein p49/GST was verified using SDS-PAGE.The purified p49/GST protein could react with mouse antisera against Tricinella sPiralis and rabbit antisera againstpurified p49/GST protein, but not with normal mouse and rabbit sera by sandwich EL1SA.Concluslon The purified fusionprotein p49/GST with high purity and good immune activity could be used for the immune aasay of trichinellosis.福建省重点（农医）项目!95－Z－15

Study on the Model both Cutting Variable-speed of the Log-core Veneer Lathe and Moving Locus Model with Constant-speed Peeling

详细分析了无卡轴旋切机的工作原理,推导了旋切过程中刀刃进给速度与圆木直径的数学模型,同时得出旋切时间的近似计算式。从中可知,旋切过程中刀刃进给速度必须按一定规律变化才能保证旋切机正常工作。建立了旋切机恒线速旋切的运动轨迹模型,推导出旋切机恒线速旋切时间的精确计算式,并在此基础上提出了旋切时间简便的近似计算式。这些研究结果均可用于指导设计和进行实际生产。Working principle of log-core veneer lathe had been analyzed in detail,and the mathematical model between the knife-carriage feeding speed and the diameter of the log core was established,in the meantime,the approximation cutting time formula was also derived.The feeding speed must be changed with continuousness and a rule,which can let the lathe work well.Moving locus model of invariable-speed peeling was also founded,precision calculating formula of invariable-speed peeling was deduced.In the same time,the simplification cutting-speed formula was also derived.These results of the research will provide a guide for the designing and the producing practice

应用ELISA 定量检测转基因玉米中

[中文文摘]应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。用建立的ELISA法对4种进口玉米产品进行了测定,实验结果得到了免疫印迹分析的验证,并与进口试剂盒方法的定量分析结果相一致,因而建立的ELISA法具有操作简便、快速特异、定量准确、经济实惠的优点,特别适合于大批量检测,有着良好的应用前景。[英文文摘]Basedontheantibody-antigen-enzymeantibodyreaction andbyusingpuriftiedBt1insecticidalcrystalproteinas both standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was established to detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, In comparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISA methodmightbeusefulforeasy,rapid,reliableandeffectiveassayinlargesamples.厦门科技计划资助项目(350222001109

Transcription of turbot Scophthalmus maximus Mx gene induced by polyinosinic-polycytidylic acid given in different ways

以POlyI∶C(又称聚肌胞)为诱导剂,分别通过腹腔注射、浸泡和投喂3种途径诱导大菱鲆SCOPHTHAlMuS MAXIMuS体内抗病毒蛋白MX基因的转录,利用半定量rT-PCr方法检测干扰素下游基因-MX基因的转录水平来确定该诱导剂的诱导效果。结果显示,以上3种途径都能高效诱导MX蛋白MrnA的转录,均在48H之后达到高峰,其中以浸泡的方式更容易诱导MX基因转录,且在120H时仍保持较高水平。MX基因转录的时相变化证明了国产POlyI∶C可以通过多种途径诱导抗病毒蛋白MX的表达,为实际应用中确定用药途径提供了理论依据。另外,实验初步建立了半定量rT-PCr方法,为检测鱼体内干扰素的表达提供了技术方法。Transcription of turbot Mx protein confirmed to be an antivirus factor was induced by PolyI∶C with intraperitoneal injection,immersion and oral administration.Mx mRNA was tested by semi-quantitative RT-PCR to validate the effect of PolyI∶C’s induction.The results illustrated that all the three induction ways could effectively induce the transcription of Mx gene and the peak of Mx mRNA quantity appeared at 48h after PolyI∶C application.The immersion method seemed to be the most effective way to induce Mx mRNA,as Mx mRNA could last more than 120h at a high level.This experiment proved that the homemade PolyI∶C could effectively induce Mx mRNA transcription in different applications,which provided a reference to use PolyI∶C in aquaculture practice.国家863计划(2003AA622070);国家十一五科技支撑计划(2006BAD09A11);行业专项(nyhyzx07-046)共同资

多重荧光PCR同时检测转基因成分35S和Nos方法的建立

根据商品化转基因作物中常用的花椰菜花叶病毒启动子 (CaMV 35S)和根癌农杆菌终止子 (Nos)的序列特点 ,设计并合成了两对引物和相对应的荧光双链探针 ,建立一种应用荧光双链探针的多重荧光PCR同时检测转基因成分 35S启动子和Nos终止子的方法 .并利用该方法对马铃薯、大豆、玉米、甜椒、番茄等 11份实物样品进行了检测 ,其中有 5份样品结果阳性 .结果表明所建立的多重荧光PCR方法能同时检测出 35S和Nos双组分 ,较常规PCR技术更为简便、快速、准确 ,有很好的应用前景

中国海及邻近区域碳库与通量综合分析

中国海总面积约470万平方公里,纵跨热带、亚热带、温带、北温带等多个气候带.其中,南海北依\"世界第三极\"青藏高原、南邻\"全球气候引擎\"西太平洋暖池,东海拥有全球最宽的陆架之一,跨陆架物质运输显著,黄海是冷暖流交汇区域,渤海则是受人类活动高度影响的内湾浅海.中国海内有长江、黄河、珠江等大河输入,外邻全球两大西边界流之一的黑潮.这些鲜明的特色赋予了中国海碳储库和通量研究的典型代表意义.文章从不同海区（渤海、黄海、东海、南海）、不同界面（陆-海、海-气、水柱-沉积物、边缘海-大洋等）,以及不同生态系统（红树林、盐沼湿地、海草床、海藻养殖、珊瑚礁、水柱生态系统等）多层面对海洋碳库与通量进行了较系统地综合分析,初步估算了各个碳库的储量与不同碳库间的通量.就海气通量而言,渤海向大气中释放CO2约0.22Tg Ca-1,黄海吸收CO2约1.15Tg Ca-1,东海吸收CO2约6.92～23.30Tg Ca-1,南海释放CO2约13.86～33.60Tg Ca-1.如果仅考虑海-气界面的CO2交换,中国海总体上是大气CO2的\"源\",净释放量约6.01～9.33Tg Ca-1.这主要是由于河流输入以及邻近大洋输入所致.河流输入渤黄海、东海、南海的溶解无机碳（DIC）分别为5.04、14.60和40.14Tg Ca-1,而邻近大洋输入DIC更是高达144.81Tg Ca-1,远超中国海向大气释放的碳量.渤海、黄海、东海、南海的沉积有机碳通量分别为2.00、3.60、7.40、7.49Tg Ca-1.东海和南海向邻近大洋输送有机碳通量分别为15.25～36.70和43.39Tg Ca-1.就生态系统而言,中国沿海红树林、盐沼湿地、海草床有机碳埋藏通量为0.36Tg Ca-1,海草床溶解有机碳（DOC）输出通量为0.59Tg Ca-1;中国近海海藻养殖移出碳通量0.68Tg Ca-1,沉积和DOC释放通量分别为0.14和0.82Tg Ca-1.总计,中国海有机碳年输出通量为81.72～103.17Tg Ca-1.中国海的有机碳输出以DOC形式为主,东海向邻近大洋输出的DOC通量约15.00～35.00Tg Ca-1,南海输出约31.39Tg Ca-1.综上,尽管从海-气通量看中国海是大气CO2的\"源\",但考虑了河流、大洋输入、沉积输出以及微型生物碳泵（DOC转化输出）作用后,中国海是重要的储碳区.需要指出的是,文章数据是基于中国海各海区碳循环研究报道,鉴于不同研究方法上的差异,所得数据难免有一定的误差范围,亟待将来统一方法标准下的更多深入研究和分析.国家重点研发计划项目(编号:2016YFA0601400);;国家自然科学基金项目(批准号:91751207、91428308、41722603、41606153、41422603);;中央高校基础研究项目(编号:20720170107);;中海油项目(编号:CNOOC-KJ125FZDXM00TJ001-2014、CNOOCKJ125FZDXM00ZJ001-2014)资