Effect of Organic Nutrition in the Nursery Growth and Nutrimental Content of Native Avocados of Ometepec, Guerrero, Mexico

In Mexico, there are several types of wild and criollo avocados that constitute a genetic heritage of this species; these avocados currently grow in an unordered manner on farmer's lands and in backyards, and they need to be studied as they are being lost because of agricultural activities and edaphoclimatic and phytosanitary factors. On the other hand, in orchards and avocado nurseries, high amounts of chemical fertilizers and pesticides are used affecting the physicochemical and microbiological properties of the soil, modifying the flora and fauna and polluting aquifers and springs that cause health problems among consumers. Therefore, it is very important to have a more friendly agriculture with the nature. The aim of this work was to evaluate under nursery conditions, the effect of organic fertilizers on 12 genotypes (rootstocks) of native avocados of Ometepec, Guerrero, Mexico, under an experimental design of random blocks, with four treatments: T1: sheep manure,T2: Bovine manure, T3: mycorrhizae and T4 (control: water) in four replicates. The variables were: plant height (PH), stem diameter (SD), number of leaves: young (NYL) and mature (NML) per plant; and the content of NO3-, K+, Ca2+ and Na+ ions obtained by petiole extraction, and the chlorophyll content measured with SPAD, in young (CYL) and mature (CML) leaves. Additionally, an analysis of variance and Tukey mean tests (P ≤ 0.01 and 0.05) and LSD (P ≤ 0.05) were done. It was found that sheep manure was superior to other treatments in PH (76.7 cm), SD (7.2 mm), NYL (6.5 leaves/plant), NML (18.4 leaves/plant), CML (40.2 SPAD) and Ca2+(1495ppm). In conclusion, the sheep manure was better than the bovine, mycorrhiza and control (water) as it affected positively the behavior of rootstocks in plant height, stem diameter and number of young and mature leaves. In addition, organic nutrition showed no significant response in the chlorophyll content of young and mature leaves. Young leaves only reached 50% of the chlorophyll content compared to mature leaves

Estimation of the vertical distribution of the fine canopy fuel in Pinus sylvestris stands using low density LiDAR data

[EN] Canopy fuel load, canopy bulk density and canopy base height are structural variables used to predict crown fire initiation and spread. Direct measurement of these variables is not functional, and they are usually estimated indirectly by modelling. Advances in fire behaviour modelling require accurate and landscape scale estimates of the complete vertical distribution of canopy fuels. The goal of the present study is to model the vertical profile of available canopy fuels in Scots pine stands by using data from the Spanish national forest inventory and low-density LiDAR data (0.5 first returns m–2) provided by Spanish PNOA project (Plan Nacional de Ortofotografía Aérea). In a first step, the vertical distribution of the canopy fuel load was modelled using the Weibull probability density function. In a second step, a system of models was fitted to relate the canopy variables to Lidar-derived metrics. Models were fitted simultaneously to compensate the effects of the inherent cross-model correlation between errors. Heteroscedasticity was also analyzed, but correction in the fitting process was not necessary. The estimated canopy fuel load profiles from LiDAR-derived metrics explained 41% of the variation in canopy fuel load in the analysed plots. The proposed models can be used to assess the effectiveness of different forest management alternatives for reducing crown fire hazard.[ES] La altura de la base de la copa, la carga de combustible disponible y la densidad aparente son características estructurales del dosel de copas utilizadas para predecir la actividad de fuego de copas. La medición directa en campo de estas variables es impráctica y por tanto sus valores se estiman habitualmente mediante el empleo de modelos predictivos. Avances en la modelización del comportamiento del fuego hacen que sea de gran interés explorar la posibilidad de estimar de forma precisa y a escala de paisaje la distribución vertical de los combustibles en el dosel de copas. En este sentido, este estudio pretende analizar el potencial de los datos obtenidos de sensores LiDAR (Light Detection and Ranging) aerotransportados para modelizar dicha distribución vertical en masas de pino silvestre en Galicia. Para ello se usaron datos del vuelo LiDAR del PNOA (Plan Nacional de Ortofotografía Aérea) con una densidad de 0,5 primeros retornos m–2 y datos de campo procedentes del Cuarto Inventario Forestal Nacional (IFN4). En un primer paso, la distribución vertical fue caracterizada empleando la función de densidad de probabilidad de Weibull para, en un segundo paso, ajustar un sistema de ecuaciones que relacionan las variables del dosel con métricas derivadas de los datos LiDAR. Las ecuaciones se ajustaron simultáneamente para corregir los posibles problemas de correlación entre errores. Las distribuciones verticales finalmente estimadas explicaron el 41% de la variabilidad observada en las parcelas de estudio. El sistema de ecuaciones propuesto puede ser usado también para evaluar la efectividad de diferentes alternativas de gestión del combustible para reducir el riesgo de fuego de copa en rodales de pino silvestre.Fidalgo-González, LA.; Arellano-Pérez, S.; Álvarez-González, JG.; Castedo-Dorado, F.; Ruiz-González, AD.; González-Ferreiro, E. (2019). Estimación de la distribución vertical de combustibles finos del dosel de copas en masas de Pinus sylvestris empleando datos LiDAR de baja densidad. Revista de Teledetección. (53):1-16. https://doi.org/10.4995/raet.2019.11241SWORD1165

Estimación de la distribución vertical de combustibles finos del dosel de copas en masas de Pinus sylvestris empleando datos LiDAR de baja densidad

[ES] La altura de la base de la copa, la carga de combustible disponible y la densidad aparente son características estructurales del dosel de copas utilizadas para predecir la actividad de fuego de copas. La medición directa en campo de estas variables es impráctica y por tanto sus valores se estiman habitualmente mediante el empleo de modelos predictivos. Avances en la modelización del comportamiento del fuego hacen que sea de gran interés explorar la posibilidad de estimar de forma precisa y a escala de paisaje la distribución vertical de los combustibles en el dosel de copas. En este sentido, este estudio pretende analizar el potencial de los datos obtenidos de sensores LiDAR (Light Detection and Ranging) aerotransportados para modelizar dicha distribución vertical en masas de pino silvestre en Galicia. Para ello se usaron datos del vuelo LiDAR del PNOA (Plan Nacional de Ortofotografía Aérea) con una densidad de 0,5 primeros retornos m–2 y datos de campo procedentes del Cuarto Inventario Forestal Nacional (IFN4). En un primer paso, la distribución vertical fue caracterizada empleando la función de densidad de probabilidad de Weibull para, en un segundo paso, ajustar un sistema de ecuaciones que relacionan las variables del dosel con métricas derivadas de los datos LiDAR. Las ecuaciones se ajustaron simultáneamente para corregir los posibles problemas de correlación entre errores. Las distribuciones verticales finalmente estimadas explicaron el 41% de la variabilidad observada en las parcelas de estudio. El sistema de ecuaciones propuesto puede ser usado también para evaluar la efectividad de diferentes alternativas de gestión del combustible para reducir el riesgo de fuego de copa en rodales de pino silvestre[EN] Canopy fuel load, canopy bulk density and canopy base height are structural variables used to predict crown fire initiation and spread. Direct measurement of these variables is not functional, and they are usually estimated indirectly by modelling. Advances in fire behaviour modelling require accurate and landscape scale estimates of the complete vertical distribution of canopy fuels. The goal of the present study is to model the vertical profile of available canopy fuels in Scots pine stands by using data from the Spanish national forest inventory and low-density LiDAR data (0.5 first returns m–2) provided by Spanish PNOA project (Plan Nacional de Ortofotografía Aérea). In a first step, the vertical distribution of the canopy fuel load was modelled using the Weibull probability density function. In a second step, a system of models was fitted to relate the canopy variables to Lidar-derived metrics. Models were fitted simultaneously to compensate the effects of the inherent cross-model correlation between errors. Heteroscedasticity was also analyzed, but correction in the fitting process was not necessary. The estimated canopy fuel load profiles from LiDAR-derived metrics explained 41% of the variation in canopy fuel load in the analysed plots. The proposed models can be used to assess the effectiveness of different forest management alternatives for reducing crown fire hazardS

Efecto de la temperatura y pH sobre la actividad y estabilidad de pectinasas producidas por Bacillus spp.

    Las pectinasas se encuentran ampliamente distribuidas en la naturaleza, como por ejemplo en productos de desecho, y su utilidad industrial es de gran importancia. En la presente investigación se trata de establecer algunos parámetros cinéticos a nivel de laboratorio para la producción de pectinasas por Bacillus spp. Se aisló y seleccionó cultivos puros de Bacillus spp. productores de la enzima a partir de muestras de tierra de cultivo de papa y naranja en medio agar nutritivo con sales y pectina como sustrato, se incubó y se realizó la lectura agregando alícuotas de una solución de lugol y aquellos cultivos que presentaban mayor halo de hidrólisis de pectina fueron seleccionados. Estos cultivos se sembraron en medio líquido nutritivo más sales y pectina y se incubaron por 24 – 48 horas; luego se centrifugaron y se obtuvo el sobrenadante denominado extracto crudo de pectinasa (ECP), el cual sirvió para realizar los ensayos de actividad y estabilidad del ECP a pHs y temperaturas variables.  Se encontró que el pH y temperatura de máxima actividad del ECP está alrededor de 6,5 y 35°C, respectivamente. El ECP es estable a pH 6,5 y 8,5 a 35°C por 120 minutos, lo mismo es cierto a temperatura de 50°C hasta por 129 minutos y pierde su estabilidad a 70°C a los 120 minutos.Palabras clave: Bacillus, pectinasas, pH, temperatura, actividad y estabilidad

Effect of having private health insurance on the use of health care services: the case of Spain

Background: Several stakeholders have undertaken initiatives to propose solutions towards a more sustainable health system and Spain, as an example of a European country affected by austerity measures, is looking for ways to cut healthcare budgets. Methods: The aim of this paper is to study the effect of private health insurance on health care utilization using the latest micro-data from the European Community Household Panel (ECHP), the Spanish National Health Survey (SNHS) and the European Union Statistics on Income and Living Conditions (EU-SILC). We use matching techniques based on propensity score methods: single match, four matches, bias-adjustment and allowing for heteroskedasticity. Results: The results demonstrate that people with a private health insurance, use the public health system less than individuals without double health insurance coverage. Conclusions: Our conclusions are useful when policy makers design public-private partnership policie

Yeasts associated with the production of distilled alcoholic beverages

Distilled alcoholic beverages are produced firstly by fermenting sugars emanating from cereal starches (in the case of whiskies), sucrose-rich plants (in the case of rums), fructooligosaccharide-rich plants (in the case of tequila) or from fruits (in the case of brandies). Traditionally, such fermentations were conducted in a spontaneous fashion, relying on indigenous microbiota, including wild yeasts. In modern practices, selected strains of Saccharomyces cerevisiae are employed to produce high levels of ethanol together with numerous secondary metabolites (eg. higher alcohols, esters, carbonyls etc.) which greatly influence the final flavour and aroma characteristics of spirits following distillation of the fermented wash. Therefore, distillers, like winemakers, must carefully choose their yeast strain which will be very important in providing the alcohol content and the sensory profiles of spirit beverages. This Chapter discusses yeast and fermentation aspects associated with the production of selected distilled spirits and highlights similarities and differences with the production of wine

Assessment of a New ROS1 Immunohistochemistry Clone (SP384) for the Identification of ROS1 Rearrangements in Patients with Non–Small Cell Lung Carcinoma: the ROSING Study

Introduction: The ROS1 gene rearrangement has become an important biomarker in NSCLC. The College of American Pathologists/International Association for the Study of Lung Cancer/Association for Molecular Pathology testing guidelines support the use of ROS1 immunohistochemistry (IHC) as a screening test, followed by confirmation with fluorescence in situ hybridization (FISH) or a molecular test in all positive results. We have evaluated a novel anti-ROS1 IHC antibody (SP384) in a large multicenter series to obtain real-world data. Methods: A total of 43 ROS1 FISH-positive and 193 ROS1 FISH-negative NSCLC samples were studied. All specimens were screened by using two antibodies (clone D4D6 from Cell Signaling Technology and clone SP384 from Ventana Medical Systems), and the different interpretation criteria were compared with break-apart FISH (Vysis). FISH-positive samples were also analyzed with next-generation sequencing (Oncomine Dx Target Test Panel, Thermo Fisher Scientific). Results: An H-score of 150 or higher or the presence of at least 70% of tumor cells with an intensity of staining of 2+ or higher by the SP384 clone was the optimal cutoff value (both with 93% sensitivity and 100% specificity). The D4D6 clone showed similar results, with an H-score of at least 100 (91% sensitivity and 100% specificity). ROS1 expression in normal lung was more frequent with use of the SP384 clone (p < 0.0001). The ezrin gene (EZR)-ROS1 variant was associated with membranous staining and an isolated green signal FISH pattern (p = 0.001 and p = 0.017, respectively). Conclusions: The new SP384 ROS1 IHC clone showed excellent sensitivity without compromising specificity, so it is another excellent analytical option for the proposed testing algorithm

Impact of mediterranean diet promotion on environmental sustainability: a longitudinal analysis

[EN]This article aims to estimate the differences in environmental impact (greenhouse gas [GHG] emissions, land use, energy used, acidification and potential eutrophication) after one year of promoting a Mediterranean diet (MD). Methods Baseline and 1-year follow-up data from 5800 participants in the PREDIMED-Plus study were used. Each participant's food intake was estimated using validated semi-quantitative food frequency questionnaires, and the adherence to MD using the Dietary Score. The influence of diet on environmental impact was assessed through the EAT-Lancet Commission tables. The influence of diet on environmental impact was assessed through the EAT-Lancet Commission tables. The association between MD adherence and its environmental impact was calculated using adjusted multivariate linear regression models.SIPublicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCL

RICORS2040 : The need for collaborative research in chronic kidney disease

Chronic kidney disease (CKD) is a silent and poorly known killer. The current concept of CKD is relatively young and uptake by the public, physicians and health authorities is not widespread. Physicians still confuse CKD with chronic kidney insufficiency or failure. For the wider public and health authorities, CKD evokes kidney replacement therapy (KRT). In Spain, the prevalence of KRT is 0.13%. Thus health authorities may consider CKD a non-issue: very few persons eventually need KRT and, for those in whom kidneys fail, the problem is 'solved' by dialysis or kidney transplantation. However, KRT is the tip of the iceberg in the burden of CKD. The main burden of CKD is accelerated ageing and premature death. The cut-off points for kidney function and kidney damage indexes that define CKD also mark an increased risk for all-cause premature death. CKD is the most prevalent risk factor for lethal coronavirus disease 2019 (COVID-19) and the factor that most increases the risk of death in COVID-19, after old age. Men and women undergoing KRT still have an annual mortality that is 10- to 100-fold higher than similar-age peers, and life expectancy is shortened by ~40 years for young persons on dialysis and by 15 years for young persons with a functioning kidney graft. CKD is expected to become the fifth greatest global cause of death by 2040 and the second greatest cause of death in Spain before the end of the century, a time when one in four Spaniards will have CKD. However, by 2022, CKD will become the only top-15 global predicted cause of death that is not supported by a dedicated well-funded Centres for Biomedical Research (CIBER) network structure in Spain. Realizing the underestimation of the CKD burden of disease by health authorities, the Decade of the Kidney initiative for 2020-2030 was launched by the American Association of Kidney Patients and the European Kidney Health Alliance. Leading Spanish kidney researchers grouped in the kidney collaborative research network Red de Investigación Renal have now applied for the Redes de Investigación Cooperativa Orientadas a Resultados en Salud (RICORS) call for collaborative research in Spain with the support of the Spanish Society of Nephrology, Federación Nacional de Asociaciones para la Lucha Contra las Enfermedades del Riñón and ONT: RICORS2040 aims to prevent the dire predictions for the global 2040 burden of CKD from becoming true

Search for Eccentric Black Hole Coalescences during the Third Observing Run of LIGO and Virgo

Despite the growing number of confident binary black hole coalescences observed through gravitational waves so far, the astrophysical origin of these binaries remains uncertain. Orbital eccentricity is one of the clearest tracers of binary formation channels. Identifying binary eccentricity, however, remains challenging due to the limited availability of gravitational waveforms that include effects of eccentricity. Here, we present observational results for a waveform-independent search sensitive to eccentric black hole coalescences, covering the third observing run (O3) of the LIGO and Virgo detectors. We identified no new high-significance candidates beyond those that were already identified with searches focusing on quasi-circular binaries. We determine the sensitivity of our search to high-mass (total mass $M>70$ $M_\odot$) binaries covering eccentricities up to 0.3 at 15 Hz orbital frequency, and use this to compare model predictions to search results. Assuming all detections are indeed quasi-circular, for our fiducial population model, we place an upper limit for the merger rate density of high-mass binaries with eccentricities $0 < e \leq 0.3$ at $0.33$ Gpc$^{-3}$ yr$^{-1}$ at 90\% confidence level.Comment: 24 pages, 5 figure