1,794 research outputs found
Rate of adaptation in sexuals and asexuals: A solvable model of the Fisher-Muller effect
The adaptation of large asexual populations is hampered by the competition
between independently arising beneficial mutations in different individuals,
which is known as clonal interference. Fisher and Muller proposed that
recombination provides an evolutionary advantage in large populations by
alleviating this competition. Based on recent progress in quantifying the speed
of adaptation in asexual populations undergoing clonal interference, we present
a detailed analysis of the Fisher-Muller mechanism for a model genome
consisting of two loci with an infinite number of beneficial alleles each and
multiplicative fitness effects. We solve the infinite population dynamics
exactly and show that, for a particular, natural mutation scheme, the speed of
adaptation in sexuals is twice as large as in asexuals. Guided by the infinite
population result and by previous work on asexual adaptation, we postulate an
expression for the speed of adaptation in finite sexual populations that agrees
with numerical simulations over a wide range of population sizes and
recombination rates. The ratio of the sexual to asexual adaptation speed is a
function of population size that increases in the clonal interference regime
and approaches 2 for extremely large populations. The simulations also show
that the imbalance between the numbers of accumulated mutations at the two loci
is strongly suppressed even by a small amount of recombination. The
generalization of the model to an arbitrary number of loci is briefly
discussed. If each offspring samples the alleles at each locus from the gene
pool of the whole population rather than from two parents, the ratio of the
sexual to asexual adaptation speed is approximately equal to in large
populations. A possible realization of this scenario is the reassortment of
genetic material in RNA viruses with genomic segments.Comment: Title has been changed. Supporting Information (animation) can be
found in the source file. 53 pages. 10 figures. To appear in Genetic
Adaptive evolution of transcription factor binding sites
The regulation of a gene depends on the binding of transcription factors to
specific sites located in the regulatory region of the gene. The generation of
these binding sites and of cooperativity between them are essential building
blocks in the evolution of complex regulatory networks. We study a theoretical
model for the sequence evolution of binding sites by point mutations. The
approach is based on biophysical models for the binding of transcription
factors to DNA. Hence we derive empirically grounded fitness landscapes, which
enter a population genetics model including mutations, genetic drift, and
selection. We show that the selection for factor binding generically leads to
specific correlations between nucleotide frequencies at different positions of
a binding site. We demonstrate the possibility of rapid adaptive evolution
generating a new binding site for a given transcription factor by point
mutations. The evolutionary time required is estimated in terms of the neutral
(background) mutation rate, the selection coefficient, and the effective
population size. The efficiency of binding site formation is seen to depend on
two joint conditions: the binding site motif must be short enough and the
promoter region must be long enough. These constraints on promoter architecture
are indeed seen in eukaryotic systems. Furthermore, we analyse the adaptive
evolution of genetic switches and of signal integration through binding
cooperativity between different sites. Experimental tests of this picture
involving the statistics of polymorphisms and phylogenies of sites are
discussed.Comment: published versio
Role of Proteome Physical Chemistry in Cell Behavior.
We review how major cell behaviors, such as bacterial growth laws, are derived from the physical chemistry of the cell's proteins. On one hand, cell actions depend on the individual biological functionalities of their many genes and proteins. On the other hand, the common physics among proteins can be as important as the unique biology that distinguishes them. For example, bacterial growth rates depend strongly on temperature. This dependence can be explained by the folding stabilities across a cell's proteome. Such modeling explains how thermophilic and mesophilic organisms differ, and how oxidative damage of highly charged proteins can lead to unfolding and aggregation in aging cells. Cells have characteristic time scales. For example, E. coli can duplicate as fast as 2-3 times per hour. These time scales can be explained by protein dynamics (the rates of synthesis and degradation, folding, and diffusional transport). It rationalizes how bacterial growth is slowed down by added salt. In the same way that the behaviors of inanimate materials can be expressed in terms of the statistical distributions of atoms and molecules, some cell behaviors can be expressed in terms of distributions of protein properties, giving insights into the microscopic basis of growth laws in simple cells
Learning dynamical information from static protein and sequencing data
Many complex processes, from protein folding to neuronal network dynamics, can be described as stochastic exploration of a high-dimensional energy landscape. While efficient algorithms for cluster detection in high-dimensional spaces have been developed over the last two decades, considerably less is known about the reliable inference of state transition dynamics in such settings. Here, we introduce a flexible and robust numerical framework to infer Markovian transition networks directly from time-independent data sampled from stationary equilibrium distributions. We demonstrate the practical potential of the inference scheme by reconstructing the network dynamics for several protein folding transitions, gene-regulatory network motifs and HIV evolution pathways. The predicted network topologies and relative transition time scales agree well with direct estimates from time-dependent molecular dynamics data, stochastic simulations and phylogenetic trees, respectively. Owing to its generic structure, the framework introduced here will be applicable to high-throughput RNA and protein sequencing datasets and future cryo-electronmicroscopy data
Magnitude and sign epistasis among deleterious mutations in a positive-sense plant RNA virus
How epistatic interactions between mutations determine the genetic architecture of fitness is of central importance in evolution. The study of epistasis is particularly interesting for RNA viruses because of their genomic compactness, lack of genetic redundancy, and apparent low complexity. Moreover, interactions between mutations in viral genomes determine traits such as resistance to antiviral drugs, virulence and host range. In this study we generated 53 Tobacco etch potyvirus genotypes carrying pairs of single-nucleotide substitutions and measured their separated and combined deleterious fitness effects. We found that up to 38% of pairs had significant epistasis for fitness, including both positive and negative deviations from the null hypothesis of multiplicative effects. Interestingly, the sign of epistasis was correlated with viral protein-protein interactions in a model network, being predominantly positive between linked pairs of proteins and negative between unlinked ones. Furthermore, 55% of significant interactions were cases of reciprocal sign epistasis (RSE), indicating that adaptive landscapes for RNA viruses maybe highly rugged. Finally, we found that the magnitude of epistasis correlated negatively with the average effect of mutations. Overall, our results are in good agreement to those previously reported for other viruses and further consolidate the view that positive epistasis is the norm for small and compact genomes that lack genetic robustness.Spanish Ministry of Science and Innovation
BFU2009-06993
JAE program from CSICPeer reviewe
On the networked architecture of genotype spaces and its critical effects on molecular evolution
Evolutionary dynamics is often viewed as a subtle process of change accumulation that causes a divergence among organisms and their genomes. However, this interpretation is an inheritance of a gradualistic view that has been challenged at the macroevolutionary, ecological and molecular level. Actually, when the complex architecture of genotype spaces is taken into account, the evolutionary dynamics of molecular populations becomes intrinsically non-uniform, sharing deep qualitative and quantitative similarities with slowly driven physical systems: nonlinear responses analogous to critical transitions, sudden state changes or hysteresis, among others. Furthermore, the phenotypic plasticity inherent to genotypes transforms classical fitness landscapes into multiscapes where adaptation in response to an environmental change may be very fast. The quantitative nature of adaptive molecular processes is deeply dependent on a network-of-networks multilayered structure of the map from genotype to function that we begin to unveil.This work has been supported by the Spanish Ministerio de Economía y Competitividad and FEDER funds of the EU through grants ViralESS (FIS2014-57686-P) and VARIANCE (FIS2015-64349-P). J.A. is supported through grant no. SEV-2013-0347. P.C. is supported through the European Union's YEI funds
The impact of population size on the evolution of asexual microbes on smooth versus rugged fitness landscapes
<p>Abstract</p> <p>Background</p> <p>It is commonly thought that large asexual populations evolve more rapidly than smaller ones, due to their increased rate of beneficial mutations. Less clear is how population size influences the level of fitness an asexual population can attain. Here, we simulate the evolution of bacteria in repeated serial passage experiments to explore how features such as fitness landscape ruggedness, the size of the mutational target under selection, and the mutation supply rate, interact to affect the evolution of microbial populations of different sizes.</p> <p>Results</p> <p>We find that if the fitness landscape has many local peaks, there can be a trade-off between the rate of adaptation and the potential to reach high fitness peaks. This result derives from the fact that whereas large populations evolve mostly deterministically and often become trapped on local fitness peaks, smaller populations can follow more stochastic evolutionary paths and thus locate higher fitness peaks. We also find that the target size of adaptation and the mutation rate interact with population size to influence the trade-off between rate of adaptation and final fitness.</p> <p>Conclusion</p> <p>Our study suggests that the optimal population size for adaptation depends on the details of the environment and on the importance of either the ability to evolve rapidly or to reach high fitness levels.</p
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