446 research outputs found

    First Experience with a Camera Collar in a Free-Ranging Przewalski’s Horse Group in the Mongolian Gobi

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    Remote sensing and satellite telemetry have allowed to greatly expanding the understanding of how species use various landscapes, even in remote settings. However, remotely collecting data also harbors the risk of losing “touch with the ground.” We explore the possibility of the additional insight cameras integrated in GPS-satellite collars can provide for the behavior and ecology of free-ranging Przewalski’s horse in the remote Great Gobi B Strictly Protected Area in southeastern Mongolia. Over a 91-day period, the camera collected 1,080 images. 62% of the images showed Przewalski’s horses and provided insights into behavior and grouping patterns and can supplement indirect measures of behavior from acceleration sensors. Other images provided first information on insect harassment and show the potential of images for ground-truthing environmental conditions, for example, the occurrence of rainfall. The potential for camera collars as an additional tool to study large-bodied ungulates in remote ecosystems seems really promising, although this relatively new technology seems still prone to technical failures

    A High Density SNP Array for the Domestic Horse and Extant Perissodactyla: Utility for Association Mapping, Genetic Diversity, and Phylogeny Studies

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    An equine SNP genotyping array was developed and evaluated on a panel of samples representing 14 domestic horse breeds and 18 evolutionarily related species. More than 54,000 polymorphic SNPs provided an average inter-SNP spacing of ∌43 kb. The mean minor allele frequency across domestic horse breeds was 0.23, and the number of polymorphic SNPs within breeds ranged from 43,287 to 52,085. Genome-wide linkage disequilibrium (LD) in most breeds declined rapidly over the first 50–100 kb and reached background levels within 1–2 Mb. The extent of LD and the level of inbreeding were highest in the Thoroughbred and lowest in the Mongolian and Quarter Horse. Multidimensional scaling (MDS) analyses demonstrated the tight grouping of individuals within most breeds, close proximity of related breeds, and less tight grouping in admixed breeds. The close relationship between the Przewalski's Horse and the domestic horse was demonstrated by pair-wise genetic distance and MDS. Genotyping of other Perissodactyla (zebras, asses, tapirs, and rhinoceros) was variably successful, with call rates and the number of polymorphic loci varying across taxa. Parsimony analysis placed the modern horse as sister taxa to Equus przewalski. The utility of the SNP array in genome-wide association was confirmed by mapping the known recessive chestnut coat color locus (MC1R) and defining a conserved haplotype of ∌750 kb across all breeds. These results demonstrate the high quality of this SNP genotyping resource, its usefulness in diverse genome analyses of the horse, and potential use in related species

    A Massively Parallel Sequencing Approach Uncovers Ancient Origins and High Genetic Variability of Endangered Przewalski's Horses

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    The endangered Przewalski's horse is the closest relative of the domestic horse and is the only true wild horse species surviving today. The question of whether Przewalski's horse is the direct progenitor of domestic horse has been hotly debated. Studies of DNA diversity within Przewalski's horses have been sparse but are urgently needed to ensure their successful reintroduction to the wild. In an attempt to resolve the controversy surrounding the phylogenetic position and genetic diversity of Przewalski's horses, we used massively parallel sequencing technology to decipher the complete mitochondrial and partial nuclear genomes for all four surviving maternal lineages of Przewalski's horses. Unlike single-nucleotide polymorphism (SNP) typing usually affected by ascertainment bias, the present method is expected to be largely unbiased. Three mitochondrial haplotypes were discovered—two similar ones, haplotypes I/II, and one substantially divergent from the other two, haplotype III. Haplotypes I/II versus III did not cluster together on a phylogenetic tree, rejecting the monophyly of Przewalski's horse maternal lineages, and were estimated to split 0.117–0.186 Ma, significantly preceding horse domestication. In the phylogeny based on autosomal sequences, Przewalski's horses formed a monophyletic clade, separate from the Thoroughbred domestic horse lineage. Our results suggest that Przewalski's horses have ancient origins and are not the direct progenitors of domestic horses. The analysis of the vast amount of sequence data presented here suggests that Przewalski's and domestic horse lineages diverged at least 0.117 Ma but since then have retained ancestral genetic polymorphism and/or experienced gene flow

    Horses as Sources of Proprietary Information: Commercialization, Conservation, and Compensation Pursuant to the Convention on Biological Diversity

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    Horses indigenous to East and Southeast (E/SE) Asia, including native, landrace, feral, and wild populations, embody valuable genetic diversity. Conservation efforts for animals have largely been driven by humane altruism, with little consideration for the information value of genomes. Yet, if horses are viewed as archives of information as well as objects of affection, their conservation shifts to a market-based paradigm. Horse genetic resources (GR) likely contain significant value to the lucrative global horse industry, including veterinary applications such as diagnostics, therapeutics, genetic markers, gene therapies, and cloning technologies. As biotechnology becomes increasingly sophisticated, mining of horse GR will accelerate, thus facilitating identification, inventorying, bioprospecting, and commercialization of genetic information. Yet, establishing a value chain that balances equitable compensation for commercial applications while promoting conservation of horse populations remains a challenge. Recommendations presented here include establishing regional and national human resource and institutional capacity (competent national authorities), that catalog eco-geographical inventories of horse GR; monitor, manage, market and direct equitable value chains from horse to genetic information to commercial products; and ensure revenue flow back to support conservation. This system will foster market incentives to build capacity for sustainable conservation of the diverse horse populations of E/SE Asia

    On the Move – Do Domestic and Wild Ungulate Species Distributions Overlap in the Mongolian Gobi?

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    In the Great Gobi B Strictly Protected Area (Great Gobi B), wild and domestic ungulates seasonally share the forage of the semi-desert and desert habitat. Around 130 herder families are grazing their livestock, mainly goats and sheep, in the protected area in winter. Wild ungulates of global significance in Great Gobi B include the reintroduced Przewalski’s horse (Equus ferus przewalskii),which had previously been extinct in the wild. To determine potential habitat overlaps between Przewalski’s horses and livestock, we mapped the movements of 19 livestock herds monitored via GPS collars and ranger observations of Przewalski’s horse herds over a one year period from September 2018 to August 2019. We additionally conducted focus group interviews with nomadic herders about their rangeland management. We found that pasture use in and around the Great Gobi B is still following the nomadic tradition, with herders moving camp locations on average eleven times per year, depending on forage availability. Our results show that the range of Przewalski’s horses and livestock mostly overlap around permanent and ephemeral water points. However, the same resources are used in different seasons. The protected area was recently expanded to twice its size, now also including additional herder households and traditional pastures. For the ongoing discussion about concerning the new zonation of the enlarged protected area it is important to consider both, herder and wildlife movements patterns, to meet the conservation goals of the protected area but also meet the needs of the traditional pastoral herding community

    Identification of Herder-Wild Equid Conflicts in the Great Gobi B Strictly Protected Area in SW Mongolia

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    In protected areas of Mongolian overgrazing, competition with wild ungulates and poaching are important management concerns. The Great Gobi B Strictly Protected Area (SPA) in SW Mongolia is a re-introduction site for the Przewalski’s horse (Equus ferus przewalskii), a stronghold of the Asiatic wild ass (Equus hemionus), and remains an important grazing area for seminomadic herders. We show the power of simple inventory and monitoring methods to assess herder-wildlife conflicts, by combining data of: (1) human and livestock demographic data, (2) migration patterns, of semi-nomadic herders, (3) monthly surveys of wild- and domestic ungulates, and (4) observations of re-introduced, free-ranging Przewalski’s horses. A total of 111 semi-nomadic families with 57,657 head of livestock use the park, mainly in winter. Grazing impact of small stock affects 33% of the park area and is virtually absent in the core area. However, due to the unequal distribution of open water, livestock is present at almost all water points. Seasonal wild horse and wild ass distribution seems to be positively linked to water availability and negatively to herder presence. We documented several cases of wild ungulate poaching, but the magnitude of the problem remains unknown. There are still many knowledge gaps and local people need to be more actively involvement in park management. As this is a rather new approach in Mongolia, we suggest park management to move towards adaptive co-management, accompanied by simple, but sound monitoring and evaluation schemes

    Identification of Herder-Wild Equid Conflicts in the Great Gobi B Strictly Protected Area in SW Mongolia

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    In protected areas of Mongolian overgrazing, competition with wild ungulates and poaching are important management concerns. The Great Gobi B Strictly Protected Area (SPA) in SW Mongolia is a re-introduction site for the Przewalski’s horse (Equus ferus przewalskii), a stronghold of the Asiatic wild ass (Equus hemionus), and remains an important grazing area for seminomadic herders. We show the power of simple inventory and monitoring methods to assess herder-wildlife conflicts, by combining data of: (1) human and livestock demographic data, (2) migration patterns, of semi-nomadic herders, (3) monthly surveys of wild- and domestic ungulates, and (4) observations of re-introduced, free-ranging Przewalski’s horses. A total of 111 semi-nomadic families with 57,657 head of livestock use the park, mainly in winter. Grazing impact of small stock affects 33% of the park area and is virtually absent in the core area. However, due to the unequal distribution of open water, livestock is present at almost all water points. Seasonal wild horse and wild ass distribution seems to be positively linked to water availability and negatively to herder presence. We documented several cases of wild ungulate poaching, but the magnitude of the problem remains unknown. There are still many knowledge gaps and local people need to be more actively involvement in park management. As this is a rather new approach in Mongolia, we suggest park management to move towards adaptive co-management, accompanied by simple, but sound monitoring and evaluation schemes

    Understanding the Reproductive Biology of the Przewalski's Horse (Equus ferus przewalskii)

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    The Przewalski's horse (Equus ferus przewalskii) once roamed the Eurasian Steppe but is now considered Critically Endangered with only 1872 individuals remaining in the world, representing progeny from only 14 founder animals (Lee and Boyd, 2008). Genetic diversity needs to be optimal for long term survival of this species. Unfortunately, increasing genetic diversity of the captive population in North America has been hindered by a decrease in fertility. Therefore, the main focus of this research was to characterize reproductive parameters in Przewalski's horse, including estrus cycle in mares and seminal traits in stallions, and determining whether age or inbreeding had an impact on these traits. A secondary focus was to determine whether hormone manipulation of the estrous cycle in mares could be utilized for the long-term goal of using artificial insemination as a breeding management tool for this species. To facilitate these studies, a technique for palpation of Przewalski's mares was developed; the first application of such a procedure in a wild equid. Subsequently, we were able to describe follicular changes in relation to urinary hormone patterns. Fifty percent of the mares had either irregular or acyclic hormonal and follicular patterns. These patterns were directly correlated with inbreeding which is the first time such a correlation has been described in this species. Estrous manipulation was possible using an injectable biorelease form of the progestagen, altrenogest. In stallions, we developed a reliable method of semen collection for Przewalski's stallions and, as a result, describe seminal traits from 98 semen collections from 14 stallions. Based on these collections, we were able to show that sub-fertility in this population could be due to the low percentage of normal spermatozoa. Based on variable analysis, seminal traits total concentration, volume and morphology showed variable changes through the year. Traits also varied on an individual stallion basis. Together, these studies demonstrated that inbreeding is detrimentally affecting the reproductive fitness of this species and that aggressive management is needed for long term sustainability of the captive population
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