Establishing What Constitutes a Healthy Human Gut Microbiome: State of the Science, Regulatory Considerations, and Future Directions.

On December 17, 2018, the North American branch of the International Life Sciences Institute (ILSI North America) convened a workshop "Can We Begin to Define a Healthy Gut Microbiome Through Quantifiable Characteristics?" with >40 invited academic, government, and industry experts in Washington, DC. The workshop objectives were to 1) develop a collective expert assessment of the state of the evidence on the human gut microbiome and associated human health benefits, 2) see if there was sufficient evidence to establish measurable gut microbiome characteristics that could serve as indicators of "health," 3) identify short- and long-term research needs to fully characterize healthy gut microbiome-host relationships, and 4) publish the findings. Conclusions were as follows: 1) mechanistic links of specific changes in gut microbiome structure with function or markers of human health are not yet established; 2) it is not established if dysbiosis is a cause, consequence, or both of changes in human gut epithelial function and disease; 3) microbiome communities are highly individualized, show a high degree of interindividual variation to perturbation, and tend to be stable over years; 4) the complexity of microbiome-host interactions requires a comprehensive, multidisciplinary research agenda to elucidate relationships between gut microbiome and host health; 5) biomarkers and/or surrogate indicators of host function and pathogenic processes based on the microbiome need to be determined and validated, along with normal ranges, using approaches similar to those used to establish biomarkers and/or surrogate indicators based on host metabolic phenotypes; 6) future studies measuring responses to an exposure or intervention need to combine validated microbiome-related biomarkers and/or surrogate indicators with multiomics characterization of the microbiome; and 7) because static genetic sampling misses important short- and long-term microbiome-related dynamic changes to host health, future studies must be powered to account for inter- and intraindividual variation and should use repeated measures within individuals

Higher In vitro Proliferation Rate of Rhizopus oryzae in Blood of Diabetic Individuals in Chronic Glycaemic Control Compared with Non-diabetic Individuals

We thank all members of the Laboratory of Clinical Microbiology in the Hospital General Dr. Manuel Gea González, Instituto Nacional de Rehabilitacion and Hospital General de México. Also, thanks to the Wellcome Trust Strategic Award, corresponding author’s scholarship sponsor. Financial Support This study did not have pharmaceutical or grant support, and resources were obtained from institutional budgets.Peer reviewedPublisher PD

From internet architecture research to standards

Many Internet architectural research initiatives have been undertaken over last twenty years. None of them actually reached their intended goal: the evolution of the Internet architecture is still driven by its protocols not by genuine architectural evolutions. As this approach becomes the main limiting factor of Internet growth and application deployment, this paper proposes an alternative research path starting from the root causes (the progressive depletion of the design principles of the Internet) and motivates the need for a common architectural foundation. For this purpose, it proposes a practical methodology to incubate architectural research results as part of the standardization process

Variation in honey bee gut microbial diversity affected by ontogenetic stage, age and geographic location

Social honey bees, Apis mellifera, host a set of distinct microbiota, which is similar across the continents and various honey bee species. Some of these bacteria, such as lactobacilli, have been linked to immunity and defence against pathogens. Pathogen defence is crucial, particularly in larval stages, as many pathogens affect the brood. However, information on larval microbiota is conflicting. Seven developmental stages and drones were sampled from 3 colonies at each of the 4 geographic locations of A. mellifera carnica, and the samples were maintained separately for analysis. We analysed the variation and abundance of important bacterial groups and taxa in the collected bees. Major bacterial groups were evaluated over the entire life of honey bee individuals, where digestive tracts of same aged bees were sampled in the course of time. The results showed that the microbial tract of 6-day-old 5th instar larvae were nearly equally rich in total microbial counts per total digestive tract weight as foraging bees, showing a high percentage of various lactobacilli (Firmicutes) and Gilliamella apicola (Gammaproteobacteria 1). However, during pupation, microbial counts were significantly reduced but recovered quickly by 6 days post-emergence. Between emergence and day 6, imago reached the highest counts of Firmicutes and Gammaproteobacteria, which then gradually declined with bee age. Redundancy analysis conducted using denaturing gradient gel electrophoresis identified bacterial species that were characteristic of each developmental stage. The results suggest that 3-day 4th instar larvae contain low microbial counts that increase 2-fold by day 6 and then decrease during pupation. Microbial succession of the imago begins soon after emergence. We found that bacterial counts do not show only yearly cycles within a colony, but vary on the individual level. Sampling and pooling adult bees or 6th day larvae may lead to high errors and variability, as both of these stages may be undergoing dynamic succession

Challenges of nematode control in ruminants : focus on Latin America

AbstractGastrointestinal nematodes (GIN) of ruminants (cattle, sheep and goats) are ubiquitous and can cause severe injuries to infected animals and significant losses in farming revenues. GIN are able to survive severe environmental and host conditions, but mankind has developed a number of ingenious methods for parasite control. The commerce and use of modern anthelmintic drugs with a broad spectrum of activity has been a solid tool for nearly 40 years, however the continuous use of these drugs, has led to the selection of populations of drug-resistant worms worldwide. At present, the ever-growing agricultural systems in Latin America are facing many challenges and cannot rely on the far-reaching objective of parasitic elimination from the host or the environment. The lack of extensive programs for monitoring drug resistance exacerbates the negative consequences of reduced efficacy, which is evident in some areas with the increase in mortality rate even after treatment. Experts agree that new schemes of parasitic control are needed and should be based on the strategy of targeted selective treatment where affected hosts are identified and treated accordingly. In this article, we will focus our discussion on the challenges for the control of GIN in Latin America by 2020 imposed by reduced drug efficacy. We will evaluate phenotypic and molecular markers, methods for single-animal evaluation, and the implementation of schemes for anthelmintic treatment that address parasites in refugia

Towards a mobile computing middleware: a synergy of reflection and mobile code techniques

The increasing popularity of wireless devices, such as mobile phones, personal digital assistants, watches and the like. is enabling new classes of applications that present challenging problems to designers. Applications have to be aware of, and adapt to, frequent variations in the context of execution, such as fluctuating network bandwidth, decreasing batten, power, changes in location or device capabilities, and so on. In this paper, we argue that middleware solutions for wired distributed systems cannot be used in a mobile setting, as the principle of transparency that has driven their design runs counter to the new degrees of awareness imposed by mobility: We propose a synergy of reflection and code mobility as a means for middleware to give applications the desired level of flexibility to react to changes happening in the environment, including those that have not necessarily been foreseen by middleware designers. We ruse the sharing and processing of images as an application scenario to highlight the advantages of our approach

Experimenting with Gnutella Communities

Computer networks and distributed systems in general may be regarded as communities where the individual components, be they entire systems, application software or users, interact in a shared environment. Such communities dynamically evolve with components or nodes joning and leaving the system. Their own individual activities affect the community's behaviour and vice-versa. This paper discusses various experiments undertaken to investigate the behaviour of a real system, the Gnutella network, which represents such a community. Gnutella is a distributed Peer-to-Peer data-sharing system without any central control. It turns out that most interactions between nodes do not last long and much of their activity is devoted to finding appropriate partners in the network. Good connections lasting longer appear only as rare events. For example, out of 42,000 connections only 57 hosts were found to available on a regular basis. This means that, in contrast to the common belief that this kind of peer-to-peer networks or sub-communities are always large, they are actually quite small. However, those sub-communities examplify very dynamic behaviour because their actual composition can change very quickly. The experimental results presented have been obtained from a Java implementation of Gnutella running in the open Internet environment, and thus in unknown and quickly changing network structures heavily dependent on chance. Les réseaux informatique ainsi que les systèmes distribués peuvent être considérés comme des communautés où les composantes - que ce soit des systèmes complets, des programmes ou des usagers - interagissent dans un environnement partagé. Ces communautés sont dynamiques car des éléments peuvent s'y joindre ou quitter en tout temps. L'article présente les résultats d'une suite d'expériences et de mesures faites sur Gnutella, un système peer-to-peer à grande échelle qui opère sans aucun contrôle centralisé. Nous avons remarqué qu'une grande partie des messages échangés sont erronés ou redondants et que les interactions entre n?uds ne durent pas très longtemps. En particulier, des connexions durant plus d'une minute sont des phénomènes rares. Les n?uds passent donc la majorité de leur temps à remplacer les partenaires perdus et, contrairement à l'idée répandue que les réseaux peer-to-peer sont immenses, nous avons noté que les communautés effectives étaient assez limitées. Gnutella est un environnement très dynamique avec peu de stabilité. Par exemple, de 42,000 sites avec lesquels nous avons établi une connexion, il a seulement été possible de re-communiquer de façon régulière avec 57. Dans un tel environnement, la chance joue un rôle important dans la performance observée; mais nous avons élaboré un protocole expérimental permettant de comparer diverses options.Gnutella, peer-to-peer networks, Internet communities, distributed systems, protocols, Gnutella, réseaux peer-to-peer, communautés virtuelles, internet, systèmes distribués, protocoles de télécommunication