Principal variable selection to explain grain yield variation in winter wheat from features extracted from UAV imagery

Background: Automated phenotyping technologies are continually advancing the breeding process. However, collecting various secondary traits throughout the growing season and processing massive amounts of data still take great efforts and time. Selecting a minimum number of secondary traits that have the maximum predictive power has the potential to reduce phenotyping efforts. The objective of this study was to select principal features extracted from UAV imagery and critical growth stages that contributed the most in explaining winter wheat grain yield. Five dates of multispectral images and seven dates of RGB images were collected by a UAV system during the spring growing season in 2018. Two classes of features (variables), totaling to 172 variables, were extracted for each plot from the vegetation index and plant height maps, including pixel statistics and dynamic growth rates. A parametric algorithm, LASSO regression (the least angle and shrinkage selection operator), and a non-parametric algorithm, random forest, were applied for variable selection. The regression coefficients estimated by LASSO and the permutation importance scores provided by random forest were used to determine the ten most important variables influencing grain yield from each algorithm. Results: Both selection algorithms assigned the highest importance score to the variables related with plant height around the grain filling stage. Some vegetation indices related variables were also selected by the algorithms mainly at earlier to mid growth stages and during the senescence. Compared with the yield prediction using all 172 variables derived from measured phenotypes, using the selected variables performed comparable or even better. We also noticed that the prediction accuracy on the adapted NE lines (r = 0.58–0.81) was higher than the other lines (r = 0.21–0.59) included in this study with different genetic backgrounds. Conclusions: With the ultra-high resolution plot imagery obtained by the UAS-based phenotyping we are now able to derive more features, such as the variation of plant height or vegetation indices within a plot other than just an averaged number, that are potentially very useful for the breeding purpose. However, too many features or variables can be derived in this way. The promising results from this study suggests that the selected set from those variables can have comparable prediction accuracies on the grain yield prediction than the full set of them but possibly resulting in a better allocation of efforts and resources on phenotypic data collection and processing

High-throughput estimation of crop traits: A review of ground and aerial phenotyping platforms

Crop yields need to be improved in a sustainable manner to meet the expected worldwide increase in population over the coming decades as well as the effects of anticipated climate change. Recently, genomics-assisted breeding has become a popular approach to food security; in this regard, the crop breeding community must better link the relationships between the phenotype and the genotype. While high-throughput genotyping is feasible at a low cost, highthroughput crop phenotyping methods and data analytical capacities need to be improved. High-throughput phenotyping offers a powerful way to assess particular phenotypes in large-scale experiments, using high-tech sensors, advanced robotics, and imageprocessing systems to monitor and quantify plants in breeding nurseries and field experiments at multiple scales. In addition, new bioinformatics platforms are able to embrace large-scale, multidimensional phenotypic datasets. Through the combined analysis of phenotyping and genotyping data, environmental responses and gene functions can now be dissected at unprecedented resolution. This will aid in finding solutions to currently limited and incremental improvements in crop yields

Uumanned Aerial Vehicle Data Analysis For High-throughput Plant Phenotyping

The continuing population is placing unprecedented demands on worldwide crop yield production and quality. Improving genomic selection for breeding process is one essential aspect for solving this dilemma. Benefitted from the advances in high-throughput genotyping, researchers already gained better understanding of genetic traits. However, given the comparatively lower efficiency in current phenotyping technique, the significance of phenotypic traits has still not fully exploited in genomic selection. Therefore, improving HTPP efficiency has become an urgent task for researchers. As one of the platforms utilized for collecting HTPP data, unmanned aerial vehicle (UAV) allows high quality data to be collected within short time and by less labor. There are currently many options for customized UAV system on market; however, data analysis efficiency is still one limitation for the fully implementation of HTPP. To this end, the focus of this program was data analysis of UAV acquired data. The specific objectives were two-fold, one was to investigate statistical correlations between UAV derived phenotypic traits and manually measured sorghum biomass, nitrogen and chlorophyll content. Another was to conduct variable selection on the phenotypic parameters calculated from UAV derived vegetation index (VI) and plant height maps, aiming to find out the principal parameters that contribute most in explaining winter wheat grain yield. Corresponding, two studies were carried out. Good correlations between UAV-derived VI/plant height and sorghum biomass/nitrogen/chlorophyll in the first study suggested that UAV-based HTPP has great potential in facilitating genetic improvement. For the second study, variable selection results from the single-year data showed that plant height related parameters, especially from later season, contributed more in explaining grain yield. Advisor: Yeyin Sh

Combining Unmanned Aerial Vehicle (UAV)-Based Multispectral Imagery and Ground-Based Hyperspectral Data for Plant Nitrogen Concentration Estimation in Rice

Plant nitrogen concentration (PNC) is a critical indicator of N status for crops, and can be used for N nutrition diagnosis and management. This work aims to explore the potential of multispectral imagery from unmanned aerial vehicle (UAV) for PNC estimation and improve the estimation accuracy with hyperspectral data collected in the field with a hyperspectral radiometer. In this study we combined selected vegetation indices (VIs) and texture information to estimate PNC in rice. The VIs were calculated from ground and aerial platforms and the texture information was obtained from UAV-based multispectral imagery. Two consecutive years (2015 & 2016) of experiments were conducted, involving different N rates, planting densities and rice cultivars. Both UAV flights and ground spectral measurements were taken along with destructive samplings at critical growth stages of rice (Oryza sativa L.). After UAV imagery preprocessing, both VIs and texture measurements were calculated. Then the optimal normalized difference texture index (NDTI) from UAV imagery was determined for separated stage groups and the entire season. Results demonstrated that aerial VIs performed well only for pre-heading stages (R2 = 0.52–0.70), and photochemical reflectance index and blue N index from ground (PRIg and BNIg) performed consistently well across all growth stages (R2 = 0.48–0.65 and 0.39–0.68). Most texture measurements were weakly related to PNC, but the optimal NDTIs could explain 61 and 51% variability of PNC for separated stage groups and entire season, respectively. Moreover, stepwise multiple linear regression (SMLR) models combining aerial VIs and NDTIs did not significantly improve the accuracy of PNC estimation, while models composed of BNIg and optimal NDTIs exhibited significant improvement for PNC estimation across all growth stages. Therefore, the integration of ground-based narrow band spectral indices with UAV-based textural information might be a promising technique in crop growth monitoring

High-throughput phenotyping of plant leaf morphological, physiological, and biochemical traits on multiple scales using optical sensing

Acquisition of plant phenotypic information facilitates plant breeding, sheds light on gene action, and can be applied to optimize the quality of agricultural and forestry products. Because leaves often show the fastest responses to external environmental stimuli, leaf phenotypic traits are indicators of plant growth, health, and stress levels. Combination of new imaging sensors, image processing, and data analytics permits measurement over the full life span of plants at high temporal resolution and at several organizational levels from organs to individual plants to field populations of plants. We review the optical sensors and associated data analytics used for measuring morphological, physiological, and biochemical traits of plant leaves on multiple scales. We summarize the characteristics, advantages and limitations of optical sensing and data-processing methods applied in various plant phenotyping scenarios. Finally, we discuss the future prospects of plant leaf phenotyping research. This review aims to help researchers choose appropriate optical sensors and data processing methods to acquire plant leaf phenotypes rapidly, accurately, and cost-effectively

Monitoring Agronomic Parameters of Winter Wheat Crops with Low-Cost UAV Imagery

Monitoring the dynamics in wheat crops requires near-term observations with high spatial resolution due to the complex factors influencing wheat growth variability. We studied the prospects for monitoring the biophysical parameters and nitrogen status in wheat crops with low-cost imagery acquired from unmanned aerial vehicles (UAV) over an 11 ha field. Flight missions were conducted at approximately 50 m in altitude with a commercial copter and camera system—three missions were performed between booting and maturing of the wheat plants and one mission after tillage. Ultra-high resolution orthoimages of 1.2 cm·px−1 and surface models were generated for each mission from the standard red, green and blue (RGB) aerial images. The image variables were extracted from image tone and surface models, e.g., RGB ratios, crop coverage and plant height. During each mission, 20 plots within the wheat canopy with 1 × 1 m2 sample support were selected in the field, and the leaf area index, plant height, fresh and dry biomass and nitrogen concentrations were measured. From the generated UAV imagery, we were able to follow the changes in early senescence at the individual plant level in the wheat crops. Changes in the pattern of the wheat canopy varied drastically from one mission to the next, which supported the need for instantaneous observations, as delivered by UAV imagery. The correlations between the biophysical parameters and image variables were highly significant during each mission, and the regression models calculated with the principal components of the image variables yielded R2 values between 0.70 and 0.97. In contrast, the models of the nitrogen concentrations yielded low R2 values with the best model obtained at flowering (R2 = 0.65). The nitrogen nutrition index was calculated with an accuracy of 0.10 to 0.11 NNI for each mission. For all models, information about the surface models and image tone was important. We conclude that low-cost RGB UAV imagery will strongly aid farmers in observing biophysical characteristics, but it is limited for observing the nitrogen status within wheat crops

Crop Disease Detection Using Remote Sensing Image Analysis

Pest and crop disease threats are often estimated by complex changes in crops and the applied agricultural practices that result mainly from the increasing food demand and climate change at global level. In an attempt to explore high-end and sustainable solutions for both pest and crop disease management, remote sensing technologies have been employed, taking advantages of possible changes deriving from relative alterations in the metabolic activity of infected crops which in turn are highly associated to crop spectral reflectance properties. Recent developments applied to high resolution data acquired with remote sensing tools, offer an additional tool which is the opportunity of mapping the infected field areas in the form of patchy land areas or those areas that are susceptible to diseases. This makes easier the discrimination between healthy and diseased crops, providing an additional tool to crop monitoring. The current book brings together recent research work comprising of innovative applications that involve novel remote sensing approaches and their applications oriented to crop disease detection. The book provides an in-depth view of the developments in remote sensing and explores its potential to assess health status in crops

Principal variable selection to explain grain yield variation in winter wheat from features extracted from UAV imagery

Background: Automated phenotyping technologies are continually advancing the breeding process. However, collecting various secondary traits throughout the growing season and processing massive amounts of data still take great efforts and time. Selecting a minimum number of secondary traits that have the maximum predictive power has the potential to reduce phenotyping efforts. The objective of this study was to select principal features extracted from UAV imagery and critical growth stages that contributed the most in explaining winter wheat grain yield. Five dates of multispectral images and seven dates of RGB images were collected by a UAV system during the spring growing season in 2018. Two classes of features (variables), totaling to 172 variables, were extracted for each plot from the vegetation index and plant height maps, including pixel statistics and dynamic growth rates. A parametric algorithm, LASSO regression (the least angle and shrinkage selection operator), and a non-parametric algorithm, random forest, were applied for variable selection. The regression coefficients estimated by LASSO and the permutation importance scores provided by random forest were used to determine the ten most important variables influencing grain yield from each algorithm. Results: Both selection algorithms assigned the highest importance score to the variables related with plant height around the grain filling stage. Some vegetation indices related variables were also selected by the algorithms mainly at earlier to mid growth stages and during the senescence. Compared with the yield prediction using all 172 variables derived from measured phenotypes, using the selected variables performed comparable or even better. We also noticed that the prediction accuracy on the adapted NE lines (r = 0.58–0.81) was higher than the other lines (r = 0.21–0.59) included in this study with different genetic backgrounds. Conclusions: With the ultra-high resolution plot imagery obtained by the UAS-based phenotyping we are now able to derive more features, such as the variation of plant height or vegetation indices within a plot other than just an averaged number, that are potentially very useful for the breeding purpose. However, too many features or variables can be derived in this way. The promising results from this study suggests that the selected set from those variables can have comparable prediction accuracies on the grain yield prediction than the full set of them but possibly resulting in a better allocation of efforts and resources on phenotypic data collection and processing

Mehitamata õhusõiduki rakendamine põllukultuuride saagikuse ja maa harimisviiside tuvastamisel

A Thesis for applying for the degree of Doctor of Philosophy in Environmental Protection.Väitekiri filosoofiadoktori kraadi taotlemiseks keskkonnakaitse erialal.This thesis aims to examine how machine learning (ML) technologies have aided significant advancements in image analysis in the area of precision agriculture. These multimodal computing technologies extend the use of machine learning to a broader spectrum of data collecting and selection for the advancement of agricultural practices (Nawar et al., 2017) These techniques will assist complicated cropping systems with more informed decisions with less human intervention, and provide a scalable framework for incorporating expert knowledge of the PA system. (Chlingaryan et al., 2018). Complexity, on the other hand, can be seen as a disadvantage in crop trials, as machine learning models require training/testing databases, limited areas with insignificant sampling sizes, time and space-specificity, and environmental factor interventions, all of which complicate parameter selection and make using a single empirical model for an entire region impractical. During the early stages of writing this thesis, we used a relatively traditional machine learning method to address the regression problem of crop yield and biomass prediction [(i.e., random forest regression (RFR), support vector regression (SVR), and artificial neural network (ANN)] to predicted dry matter (DM) yields of red clover. It obtained favourable results, however, the choosing of hyperparameters, the lengthy algorithms selection process, data cleaning, and redundant collinearity issues significantly limited the way of the machine learning application. We will further discuss the recent trend of automated machine learning (AutoML) that has been driving further significant technological innovation in the application of artificial intelligence from its automated algorithm selection and hyperparameter optimization of the deployable pipeline model for unravelling substance problems. However, a present knowledge gap exists in the integration of machine learning (ML) technology with unmanned aerial systems (UAS) and hyperspectral-based imaging data categorization and regression applications. In this thesis, we explored a state-of-the-art (SOTA) and entirely open-source AutoML framework, Auto-sklearn, which was built on one of the most frequently used machine learning systems, Scikit-learn. It was integrated with two unique AutoML visualization tools to examine the recognition and acceptance of multispectral vegetation indices (VI) data collected from UAS and hyperspectral narrow-band VIs across a varied spectrum of agricultural management practices (AMP). These procedures incorporate soil tillage method (STM), cultivation method (CM), and manure application (MA), and are classified as four-crop combination fields (i.e., red clover-grass mixture, spring wheat, pea-oat mixture, and spring barley). Additionally, they have not been thoroughly evaluated and lack characteristics that are accessible in agriculture remote sensing applications. This thesis further explores the existing gaps in the knowledge base for several critical crop categories and cultivation management methods referring to biomass and yield analysis, as well as to gain a better understanding of the potential for remotely sensed solutions to field-based and multifunctional platforms to meet precision agriculture demands. To overcome these knowledge gaps, this research introduces a rapid, non-destructive, and low-cost framework for field-based biomass and grain yield modelling, as well as the identification of agricultural management practices. The results may aid agronomists and farmers in establishing more accurate agricultural methods and in monitoring environmental conditions more effectively.Doktoritöö eesmärk oli uurida, kuidas masinõppe (MÕ) tehnoloogiad võimaldavad edusamme täppispõllumajanduse valdkonna pildianalüüsis. Multimodaalsed arvutustehnoloogiad laiendavad masinõppe kasutamist põllumajanduses andmete kogumisel ja valimisel (Nawar et al., 2017). Selline täpsemal informatsioonil põhinev tehnoloogia võimaldab keerukate viljelussüsteemide puhul teha otsuseid inimese vähema sekkumisega, ja loob skaleeritava raamistiku täppispõllumajanduse jaoks (Chlingaryan et al., 2018). Põllukultuuride katsete korral on komplekssete masinõppemudelite kasutamine keerukas, sest alad on piiratud ning valimi suurus ei ole piisav; vaja on testandmebaase, kindlaid aja- ja ruumitingimusi ning keskkonnategureid. See komplitseerib parameetrite valikut ning muudab ebapraktiliseks ühe empiirilise mudeli kasutamise terves piirkonnas. Siinse uurimuse algetapis rakendati suhteliselt traditsioonilist masinõppemeetodit, et lahendada saagikuse ja biomassi prognoosimise regressiooniprobleem (otsustusmetsa regression, tugivektori regressioon ja tehisnärvivõrk) punase ristiku prognoositava kuivaine saagikuse suhtes. Saadi sobivaid tulemusi, kuid hüperparameetrite valimine, pikk algoritmide valimisprotsess, andmete puhastamine ja kollineaarsusprobleemid takistasid masinõpet oluliselt. Automatiseeritud masinõppe (AMÕ) uusimate suundumustena rakendatakse tehisintellekti, et lahendada põhiprobleemid automatiseeritud algoritmi valiku ja rakendatava pipeline-mudeli hüperparameetrite optimeerimise abil. Seni napib teadmisi MÕ tehnoloogia integreerimiseks mehitamata õhusõidukite ning hüperspektripõhiste pildiandmete kategoriseerimise ja regressioonirakendustega. Väitekirjas uuriti nüüdisaegset ja avatud lähtekoodiga AMÕ tehnoloogiat Auto-sklearn, mis on ühe enimkasutatava masinõppesüsteemi Scikit-learn edasiarendus. Süsteemiga liideti kaks unikaalset AMÕ visualiseerimisrakendust, et uurida mehitamata õhusõidukiga kogutud andmete multispektraalsete taimkatteindeksite ja hüperspektraalsete kitsaribaandmete taimkatteindeksite tuvastamist ja rakendamist põllumajanduses. Neid võtteid kasutatakse mullaharimisel, kultiveerimisel ja sõnnikuga väetamisel nelja kultuuriga põldudel (punase ristiku rohusegu, suvinisu, herne-kaera segu, suvioder). Neid ei ole põhjalikult hinnatud, samuti ei hõlma need omadusi, mida kasutatatakse põllumajanduses kaugseire rakendustes. Uurimus käsitleb biomassi ja saagikuse seni uurimata analüüsivõimalusi oluliste põllukultuuride ja viljelusmeetodite näitel. Hinnatakse ka kaugseirelahenduste potentsiaali põllupõhiste ja multifunktsionaalsete platvormide kasutamisel täppispõllumajanduses. Uurimus tutvustab kiiret, keskkonna suhtes kahjutut ja mõõduka hinnaga tehnoloogiat põllupõhise biomassi ja teraviljasaagi modelleerimiseks, et leida sobiv viljelusviis. Töö tulemused võimaldavad põllumajandustootjatel ja agronoomidel tõhusamalt valida põllundustehnoloogiaid ning arvestada täpsemalt keskkonnatingimustega.Publication of this thesis is supported by the Estonian University of Life Scieces and by the Doctoral School of Earth Sciences and Ecology created under the auspices of the European Social Fund

Wheat yellow rust monitoring by learning from multispectral UAV aerial imagery

The use of a low-cost five-band multispectral camera (RedEdge, MicaSense, USA) and a low-altitude airborne platform is investigated for the detection of plant stress caused by yellow rust disease in winter wheat for sustainable agriculture. The research is mainly focused on: (i) determining whether or not healthy and yellow rust infected wheat plants can be discriminated; (ii) selecting spectral band and Spectral Vegetation Index (SVI) with a strong discriminating capability; (iii) developing a low-cost yellow rust monitoring system for use at farmland scales. An experiment was carefully designed by infecting winter wheat with different levels of yellow rust inoculum, where aerial multispectral images under different developmental stages of yellow rust were captured by an Unmanned Aerial Vehicle at an altitude of 16–24m with a ground resolution of 1–1.5cm/pixel. An automated yellow rust detection system is developed by learning (via random forest classifier) from labelled UAV aerial multispectral imagery. Experimental results indicate that: (i) good classification performance (with an average Precision, Recall and Accuracy of 89.2%, 89.4% and 89.3%) was achieved by the developed yellow rust monitoring at a diseased stage (45 days after inoculation); (ii) the top three SVIs for separating healthy and yellow rust infected wheat plants are RVI, NDVI and OSAVI; while the top two spectral bands are NIR and Red. The learnt system was also applied to the whole farmland of interest with a promising monitoring result. It is anticipated that this study by seamlessly integrating low-cost multispectral camera, low-altitude UAV platform and machine learning techniques paves the way for yellow rust monitoring at farmland scales