1,260 research outputs found

    Image Processing Applications in Real Life: 2D Fragmented Image and Document Reassembly and Frequency Division Multiplexed Imaging

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    In this era of modern technology, image processing is one the most studied disciplines of signal processing and its applications can be found in every aspect of our daily life. In this work three main applications for image processing has been studied. In chapter 1, frequency division multiplexed imaging (FDMI), a novel idea in the field of computational photography, has been introduced. Using FDMI, multiple images are captured simultaneously in a single shot and can later be extracted from the multiplexed image. This is achieved by spatially modulating the images so that they are placed at different locations in the Fourier domain. Finally, a Texas Instruments digital micromirror device (DMD) based implementation of FDMI is presented and results are shown. Chapter 2 discusses the problem of image reassembly which is to restore an image back to its original form from its pieces after it has been fragmented due to different destructive reasons. We propose an efficient algorithm for 2D image fragment reassembly problem based on solving a variation of Longest Common Subsequence (LCS) problem. Our processing pipeline has three steps. First, the boundary of each fragment is extracted automatically; second, a novel boundary matching is performed by solving LCS to identify the best possible adjacency relationship among image fragment pairs; finally, a multi-piece global alignment is used to filter out incorrect pairwise matches and compose the final image. We perform experiments on complicated image fragment datasets and compare our results with existing methods to show the improved efficiency and robustness of our method. The problem of reassembling a hand-torn or machine-shredded document back to its original form is another useful version of the image reassembly problem. Reassembling a shredded document is different from reassembling an ordinary image because the geometric shape of fragments do not carry a lot of valuable information if the document has been machine-shredded rather than hand-torn. On the other hand, matching words and context can be used as an additional tool to help improve the task of reassembly. In the final chapter, document reassembly problem has been addressed through solving a graph optimization problem

    Development of an image converter of radical design

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    A long term investigation of thin film sensors, monolithic photo-field effect transistors, and epitaxially diffused phototransistors and photodiodes to meet requirements to produce acceptable all solid state, electronically scanned imaging system, led to the production of an advanced engineering model camera which employs a 200,000 element phototransistor array (organized in a matrix of 400 rows by 500 columns) to secure resolution comparable to commercial television. The full investigation is described for the period July 1962 through July 1972, and covers the following broad topics in detail: (1) sensor monoliths; (2) fabrication technology; (3) functional theory; (4) system methodology; and (5) deployment profile. A summary of the work and conclusions are given, along with extensive schematic diagrams of the final solid state imaging system product

    Toxicity Assays in Nanodrops Combining Bioassay and Morphometric Endpoints

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    BACKGROUND: Improved chemical hazard management such as REACH policy objective as well as drug ADMETOX prediction, while limiting the extent of animal testing, requires the development of increasingly high throughput as well as highly pertinent in vitro toxicity assays. METHODOLOGY: This report describes a new in vitro method for toxicity testing, combining cell-based assays in nanodrop Cell-on-Chip format with the use of a genetically engineered stress sensitive hepatic cell line. We tested the behavior of a stress inducible fluorescent HepG2 model in which Heat Shock Protein promoters controlled Enhanced-Green Fluorescent Protein expression upon exposure to Cadmium Chloride (CdCl(2)), Sodium Arsenate (NaAsO(2)) and Paraquat. In agreement with previous studies based on a micro-well format, we could observe a chemical-specific response, identified through differences in dynamics and amplitude. We especially determined IC50 values for CdCl(2) and NaAsO(2), in agreement with published data. Individual cell identification via image-based screening allowed us to perform multiparametric analyses. CONCLUSIONS: Using pre/sub lethal cell stress instead of cell mortality, we highlighted the high significance and the superior sensitivity of both stress promoter activation reporting and cell morphology parameters in measuring the cell response to a toxicant. These results demonstrate the first generation of high-throughput and high-content assays, capable of assessing chemical hazards in vitro within the REACH policy framework

    Supernova / Acceleration Probe: A Satellite Experiment to Study the Nature of the Dark Energy

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    The Supernova / Acceleration Probe (SNAP) is a proposed space-based experiment designed to study the dark energy and alternative explanations of the acceleration of the Universe's expansion by performing a series of complementary systematics-controlled measurements. We describe a self-consistent reference mission design for building a Type Ia supernova Hubble diagram and for performing a wide-area weak gravitational lensing study. A 2-m wide-field telescope feeds a focal plane consisting of a 0.7 square-degree imager tiled with equal areas of optical CCDs and near infrared sensors, and a high-efficiency low-resolution integral field spectrograph. The SNAP mission will obtain high-signal-to-noise calibrated light-curves and spectra for several thousand supernovae at redshifts between z=0.1 and 1.7. A wide-field survey covering one thousand square degrees resolves ~100 galaxies per square arcminute. If we assume we live in a cosmological-constant-dominated Universe, the matter density, dark energy density, and flatness of space can all be measured with SNAP supernova and weak-lensing measurements to a systematics-limited accuracy of 1%. For a flat universe, the density-to-pressure ratio of dark energy can be similarly measured to 5% for the present value w0 and ~0.1 for the time variation w'. The large survey area, depth, spatial resolution, time-sampling, and nine-band optical to NIR photometry will support additional independent and/or complementary dark-energy measurement approaches as well as a broad range of auxiliary science programs. (Abridged)Comment: 40 pages, 18 figures, submitted to PASP, http://snap.lbl.go

    Change blindness: eradication of gestalt strategies

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    Arrays of eight, texture-defined rectangles were used as stimuli in a one-shot change blindness (CB) task where there was a 50% chance that one rectangle would change orientation between two successive presentations separated by an interval. CB was eliminated by cueing the target rectangle in the first stimulus, reduced by cueing in the interval and unaffected by cueing in the second presentation. This supports the idea that a representation was formed that persisted through the interval before being 'overwritten' by the second presentation (Landman et al, 2003 Vision Research 43149–164]. Another possibility is that participants used some kind of grouping or Gestalt strategy. To test this we changed the spatial position of the rectangles in the second presentation by shifting them along imaginary spokes (by ±1 degree) emanating from the central fixation point. There was no significant difference seen in performance between this and the standard task [F(1,4)=2.565, p=0.185]. This may suggest two things: (i) Gestalt grouping is not used as a strategy in these tasks, and (ii) it gives further weight to the argument that objects may be stored and retrieved from a pre-attentional store during this task

    Holography

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    Holography - Basic Principles and Contemporary Applications is a collection of fifteen chapters, describing the basic principles of holography and some recent innovative developments in the field. The book is divided into three sections. The first, Understanding Holography, presents the principles of hologram recording illustrated with practical examples. A comprehensive review of diffraction in volume gratings and holograms is also presented. The second section, Contemporary Holographic Applications, is concerned with advanced applications of holography including sensors, holographic gratings, white-light viewable holographic stereograms. The third section of the book Digital Holography is devoted to digital hologram coding and digital holographic microscopy

    Phenotypic monitoring of cell growth and motility using image-based metrics and lensless microscopy

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    ENHANCEMENTS TO THE MODIFIED COMPOSITE PATTERN METHOD OF STRUCTURED LIGHT 3D CAPTURE

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    The use of structured light illumination techniques for three-dimensional data acquisition is, in many cases, limited to stationary subjects due to the multiple pattern projections needed for depth analysis. Traditional Composite Pattern (CP) multiplexing utilizes sinusoidal modulation of individual projection patterns to allow numerous patterns to be combined into a single image. However, due to demodulation artifacts, it is often difficult to accurately recover the subject surface contour information. On the other hand, if one were to project an image consisting of many thin, identical stripes onto the surface, one could, by isolating each stripe center, recreate a very accurate representation of surface contour. But in this case, recovery of depth information via triangulation would be quite difficult. The method described herein, Modified Composite Pattern (MCP), is a conjunction of these two concepts. Combining a traditional Composite Pattern multiplexed projection image with a pattern of thin stripes allows for accurate surface representation combined with non-ambiguous identification of projection pattern elements. In this way, it is possible to recover surface depth characteristics using only a single structured light projection. The technique described utilizes a binary structured light projection sequence (consisting of four unique images) modulated according to Composite Pattern methodology. A stripe pattern overlay is then applied to the pattern. Upon projection and imaging of the subject surface, the stripe pattern is isolated, and the composite pattern information demodulated and recovered, allowing for 3D surface representation. In this research, the MCP technique is considered specifically in the context of a Hidden Markov Process Model. Updated processing methodologies explained herein make use of the Viterbi algorithm for the purpose of optimal analysis of MCP encoded images. Additionally, we techniques are introduced which, when implemented, allow fully automated processing of the Modified Composite Pattern image
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