Mass transfer, light pulsing and hydrodynamic stress effects in photobioreactor development

Photobioreactor scalability involves multiple different interacting aspects including mass transfer, light pulsing and hydrodynamic stress. An efficient carbon dioxide supply and a frequent displacement of cells from poorly to highly illuminated zones is desired to maximise the achieveable specific growth rate. However, a strong mixing is energy consuming and may reduce the specific growth rate because of induced cell damage. The current work examines mass transfer effects in photobioreactor development and estimates their relationship to light pulsing and hydrodynamic stress effects with a special reference to the novel inclined, thinlayer, wavy-bottomed cascading photobioreactor

Renewable sustainable biocatalyzed electricity production in a photosynthetic algal microbial fuel cell (PAMFC)

Electricity production via solar energy capturing by living higher plants and microalgae in combination with microbial fuel cells are attractive because these systems promise to generate useful energy in a renewable, sustainable, and efficient manner. This study describes the proof of principle of a photosynthetic algal microbial fuel cell (PAMFC) based on naturally selected algae and electrochemically active microorganisms in an open system and without addition of instable or toxic mediators. The developed solarpowered PAMFC produced continuously over 100 days renewable biocatalyzed electricity. The sustainable performance of the PAMFC resulted in a maximum current density of 539 mA/m2 projected anode surface area and a maximum power production of 110 mW/m2 surface area photobioreactor. The energy recovery of the PAMFC can be increased by optimization of the photobioreactor, by reducing the competition from non-electrochemically active microorganisms, by increasing the electrode surface and establishment of a further-enriched biofilm. Since the objective is to produce net renewable energy with algae, future research should also focus on the development of low energy input PAMFCs. This is because current algae production systems have energy inputs similar to the energy present in the outcoming valuable products

A new photobioreactor for continuous microalgal production in hatcheries based on external-loop airlift and swirling flow

This study deals with the scale of a new photobioreactor for continuous microalgal production in hatcheries. The combination of the state-of-art with the constraints inherent to hatcheries has turned the design into a closed, artificially illuminated and external-loop airlift configuration based on a succession of elementary modules, each one being composed of two transparent vertical interconnected columns. The liquid circulation is ensured pneumatically (air injections) with respect to a swirling motion (tangential inlets). A single module of the whole photobioreactor was built-up to investigate how parameters, such as air sparger type, gas flow rate, tangential inlet, column radius and height can influence radiative transfer, hydrodynamics, mass transfer and biological performances. The volumetric productivities were predicted by modeling radiative transfer and growth of Isochrysis affinis galbana (clone Tahiti). The hydrodynamics of the liquid phase was modeled in terms of global flow behavior (circulation and mixing times, Péclet number) and of swirling motion decay along the column (Particle Image Velocimetry). The aeration performances were determined by overall volumetric mass transfer measurements. Continuous cultures of Isochrysis affinis galbana (clone Tahiti) were run in two geometrical configurations, generating either an axial or a swirling flow. Lastly, the definitive options of design are presented as well as a 120 Liter prototype, currently implemented in a French mollusk hatchery and commercialized

Design and Bench-Scale Hydrodynamic Testing of Thin-Layer Wavy Photobioreactors

In a thin-volume photobioreactor where a concentrated suspension of microalgae is circulated throughout the established spatial irradiance gradient, microalgal cells experience a time-variable irradiance. Deploying this feature is the most convenient way of obtaining the so-called flashing light effect, improving biomass production in high irradiance. This work investigates the light flashing features of sloping wavy photobioreactors, a recently proposed type, by introducing and validating a computational fluid dynamics (CFD) model. Two characteristic flow zones (straight top-to-bottom stream and local recirculation stream), both effective toward light flashing, have been found and characterized: a recirculation-induced frequency of 3.7 Hz and straight flow-induced frequency of 5.6 Hz were estimated. If the channel slope is increased, the recirculation area becomes less stable while the recirculation frequency is nearly constant with flow rate. The validated CFD model is a mighty tool that could be reliably used to further increase the flashing frequency by optimizing the design, dimensions, installation, and operational parameters of the sloping wavy photobioreactor

Periodic optimal control for biomass productivity maximization in a photobioreactor using natural light

We address the question of optimization of the microalgal biomass long term productivity in the framework of production in photobioreactors under the influence of day/night cycles. For that, we propose a simple bioreactor model accounting for light attenuation in the reactor due to biomass density and obtain the control law that optimizes productivity over a single day through the application of Pontryagin's maximum principle, with the dilution rate being the main control. An important constraint on the obtained solution is that the biomass in the reactor should be at the same level at the beginning and at the end of the day so that the same control can be applied everyday and optimizes some form of long term productivity. Several scenarios are possible depending on the microalgae's strain parameters and the maximal admissible value of the dilution rate: bang-bang or bang-singular-bang control or, if the growth rate of the algae is very strong in the presence of light, constant maximal dilution. A bifurcation diagram is presented to illustrate for which values of the parameters these different behaviors occur. Finally, a simple sub-optimal bang-bang strategy is proposed that numerically achieves productivity levels that almost match those of the optimal strategy

Assessment of carotenoid production by Dunaliella salina in different culture systems and operation regimes

The effect of operation regime and culture system on carotenoid productivity by the halotolerant alga Dunaliella salina has been analyzed. Operation strategies tested included batch and semi continuous regime, as well as a two-stage approach run simultaneously in both, open tanks and closed reactor. The best results were obtained with the closed tubular photobioreactor. The highest carotenoid production (328.8 mg carotenoid l−1 culture per month) was achieved with this culture system operated following the two-stage strategy. Also, closed tubular photobioreactor provided the highest carotenoid contents (10% of dry weight) in Dunaliella biomass and β-carotene abundance (90% of total carotenoids) as well as the highest 9-cis to all-trans β-carotene isomer ratio (1.5 at sunrise).Comisión Interministerial de Ciencia y Tecnología IFD 1997-1780IFAPA CO3-125Plan Andaluz de Investigación CVI13

Investigations in an external-loop airlift photobioreactor with annular light chambers and swirling flow

Photosynthetic microorganisms could serve as valuable compounds, but also for environmental applications. Their production under controlled conditions implies to design specific reactors, named photobioreactors, in which light supply is the main constraint. This paper was devoted to an original external-loop airlift photobioreactor (PBR) with annular light chambers in which a swirling motion was induced. The aim was to characterize this novel geometrical configuration in terms of gas–liquid hydrodynamics, and to test its potentiality for algal cultures. This PBR consisted of two identical columns connected by flanges defining tangential inlets, each column being made of two transparent concentric tubes (6 L in liquid volume, 50 m−1 in specific illuminated area). Firstly, the global flow characteristics (circulation and mixing times) were determined by a tracer method and modelled by an axial dispersed plug flow with complete recirculation (Péclet number). By means of a double optical probe, both local and global time-averaged parameters of the gas phase were measured, namely void fraction, bubble velocity, frequency and size. The gas–liquid mass transfer were also characterized, in tap water and in culture medium, by measuring overall volumetric mass transfer coefficients. In a second time, cultures of the microalga Chlamydomonas reinhardtii were run in batch mode. The variations of biomass concentration and pigment content with time from inoculation were successfully obtained. All these findings highlighted: (i) some significant differences in terms of gas–liquid hydrodynamics between the present PBR and the usual airlift systems, (ii) the interest of this configuration for algal cultures, even if complementary studies and technological improvements are still required for definitively validating its scale-up

Model-based versus model-free control designs for improving microalgae growth in a closed photobioreactor: Some preliminary comparisons

Controlling microalgae cultivation, i.e., a crucial industrial topic today, is a challenging task since the corresponding modeling is complex, highly uncertain and time-varying. A model-free control setting is therefore introduced in order to ensure a high growth of microalgae in a continuous closed photobioreactor. Computer simulations are displayed in order to compare this design to an input-output feedback linearizing control strategy, which is widely used in the academic literature on photobioreactors. They assess the superiority of the model-free standpoint both in terms of performances and implementation simplicity.Comment: The 24th Mediterranean Conference on Control and Automation (MED'16), Athens, Greece (June 21-24, 2016

Influence of liquid-to-biogas ratio and alkalinity on the biogas upgrading performance in a demo scale algal-bacterial photobioreactor

The influence of the liquid-to-biogas ratio (L/G) and alkalinity on methane quality was evaluated in a 11.7 m3 outdoors horizontal semi-closed tubular photobioreactor interconnected to a 45-L absorption column (AC). CO2 concentrations in the upgraded methane ranged from <0.1 to 9.6% at L/G of 2.0 and 0.5, respectively, with maximum CH4 concentrations of 89.7% at a L/G of 1.0. Moreover, an enhanced CO2 removal (mediating a decrease in CO2 concentration from 9.6 to 1.2%) and therefore higher CH4 contents (increasing from 88.0 to 93.2%) were observed when increasing the alkalinity of the AC cultivation broth from 42 ± 1 mg L−1 to 996 ± 42 mg L−1. H2S was completely removed regardless of the L/G or the alkalinity in AC. The continuous operation of the photobioreactor with optimized operating parameters resulted in contents of CO2 (<0.1%–1.4%), H2S (<0.7 mg m−3) and CH4 (94.1%–98.8%) complying with international regulations for methane injection into natural gas grids.Peer ReviewedPostprint (published version