154,134 research outputs found
Two center multipole expansion method: application to macromolecular systems
We propose a new theoretical method for the calculation of the interaction
energy between macromolecular systems at large distances. The method provides a
linear scaling of the computing time with the system size and is considered as
an alternative to the well known fast multipole method. Its efficiency,
accuracy and applicability to macromolecular systems is analyzed and discussed
in detail.Comment: 23 pages, 7 figures, 1 tabl
Macromolecular crowding modulates folding mechanism of alpha/beta protein apoflavodoxin
Protein dynamics in cells may be different from that in dilute solutions in
vitro since the environment in cells is highly concentrated with other
macromolecules. This volume exclusion due to macromolecular crowding is
predicted to affect both equilibrium and kinetic processes involving protein
conformational changes. To quantify macromolecular crowding effects on protein
folding mechanisms, here we have investigated the folding energy landscape of
an alpha/beta protein, apoflavodoxin, in the presence of inert macromolecular
crowding agents using in silico and in vitro approaches. By coarse-grained
molecular simulations and topology-based potential interactions, we probed the
effects of increased volume fraction of crowding agents (phi_c) as well as of
crowding agent geometry (sphere or spherocylinder) at high phi_c. Parallel
kinetic folding experiments with purified Desulfovibro desulfuricans
apoflavodoxin in vitro were performed in the presence of Ficoll (sphere) and
Dextran (spherocylinder) synthetic crowding agents. In conclusion, we have
identified in silico crowding conditions that best enhance protein stability
and discovered that upon manipulation of the crowding conditions, folding
routes experiencing topological frustrations can be either enhanced or
relieved. The test-tube experiments confirmed that apoflavodoxin's
time-resolved folding path is modulated by crowding agent geometry. We propose
that macromolecular crowding effects may be a tool for manipulation of protein
folding and function in living cells.Comment: to appear in Biophysical Journal (2009). to appear in Biophysical
Journal (2009
Molecular Dynamics Simulation of Macromolecules Using Graphics Processing Unit
Molecular dynamics (MD) simulation is a powerful computational tool to study
the behavior of macromolecular systems. But many simulations of this field are
limited in spatial or temporal scale by the available computational resource.
In recent years, graphics processing unit (GPU) provides unprecedented
computational power for scientific applications. Many MD algorithms suit with
the multithread nature of GPU. In this paper, MD algorithms for macromolecular
systems that run entirely on GPU are presented. Compared to the MD simulation
with free software GROMACS on a single CPU core, our codes achieve about 10
times speed-up on a single GPU. For validation, we have performed MD
simulations of polymer crystallization on GPU, and the results observed
perfectly agree with computations on CPU. Therefore, our single GPU codes have
already provided an inexpensive alternative for macromolecular simulations on
traditional CPU clusters and they can also be used as a basis to develop
parallel GPU programs to further speedup the computations.Comment: 21 pages, 16 figure
A new approach to high resolution, high contrast electron microscopy of macromolecular block copolymer assemblies
Determining the structure of macromolecular samples is vital for understanding and adapting their function. Transmission electron microscopy (TEM) is widely used to achieve this, but, owing to the weak electron scattering cross-section of carbon, TEM images of macromolecular samples are generally low contrast and low resolution. Here we implement a fast and practically simple routine to achieve high-contrast imaging of macromolecular samples using exit wave reconstruction (EWR), revealing a new level of structural detail. This is only possible using ultra-low contrast supports such as the graphene oxide (GO) used here and as such represents a novel application of these substrates. We apply EWR on GO membranes to study self-assembled block copolymer structures, distinguishing not only the general morphology or nanostructure, but also evidence for the substructure (i.e. the polymer chains) which gives insight into their formation mechanisms and functional properties
Stochastic dynamics of macromolecular-assembly networks
The formation and regulation of macromolecular complexes provides the
backbone of most cellular processes, including gene regulation and signal
transduction. The inherent complexity of assembling macromolecular structures
makes current computational methods strongly limited for understanding how the
physical interactions between cellular components give rise to systemic
properties of cells. Here we present a stochastic approach to study the
dynamics of networks formed by macromolecular complexes in terms of the
molecular interactions of their components. Exploiting key thermodynamic
concepts, this approach makes it possible to both estimate reaction rates and
incorporate the resulting assembly dynamics into the stochastic kinetics of
cellular networks. As prototype systems, we consider the lac operon and phage
lambda induction switches, which rely on the formation of DNA loops by proteins
and on the integration of these protein-DNA complexes into intracellular
networks. This cross-scale approach offers an effective starting point to move
forward from network diagrams, such as those of protein-protein and DNA-protein
interaction networks, to the actual dynamics of cellular processes.Comment: Open Access article available at
http://www.nature.com/msb/journal/v2/n1/full/msb4100061.htm
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