Patron Driven Acquisition: Wie wird die Ebook Library (EBL) an der Universität Leipzig genutzt?

In this study, an in-depth analysis of usage data covering one year of patron-driven acquisition on the ebook platform Ebook Library (EBL) is presented. A special focus lies on the evaluation of use and re-use of short term loans. As a result, relevant licensing parameters can be adjusted according to existing usage patterns and a more efficient operation of PDA can be achieve

Sistema De Gestión Financiera Para Mejorar El Control De Costos En Mypes De Calzado De APIAT – 2016

El presente trabajo de investigación se realizó con la finalidad de mejorar el Control de Costos de los Microempresarios de Calzado de APIAT, mediante la implementación de un Sistema de Gestión Financiera, obteniendo la información de las encuestas y el registro de la observación de sus actividades diarias de 34 microempresarios de calzado de la Asociación de Pequeños Industriales y Artesanos de Trujillo, se ha conseguido que el promedio de indicadores de gestión ha aumentado en un 26.94%, clasificando los ingresos y egresos, que le permita al microempresario tomar decisión en una determinada área, aumentando también en un 44.34% el tiempo promedio en la obtención de indicadores ha aumentado para al final evidenciar que y el nivel de satisfacción del microempresario ha aumentado en un 44.34% Como conclusión podemos afirmar que el Sistema de Gestión Financiera mejoro el control de costos de una Microempresa de Calzado de APIAT, ya que le permite llevar el registro de sus actividades económicas diarias, y reportarla ya clasificada

The underrepresentation of black and minority ethnic people in the leadership of children’s learning: chance, coincidence or design?

My research thesis reports on the under-representation of Black and minority ethnic (BAME) people in the leadership of children’s learning. The research utilises Critical Race Theory and a mixed method approach. The mixed methods involved 22 semi- structured interviews; a survey of 152 Local Authorities (LA) with responsibility for education in their area; documentary web-based research of 50 sampled LA’s Workforce Monitoring (WFM) Reports; and the use of current newspaper reports to highlight and broaden the links to inequality more generally. The purpose of the research is to answer the question of whether the under-representation of BAME people in the leadership of children’s learning, initially identified as far back as 1985, remains because of chance, coincidence or by design? The research identifies over 40 separate codes emerging from interviews with BAME leaders in children’s learning. These codes include low expectations, awareness of their position, the need to be better, monitoring, the complexity of racism and the difficulties of talking about racism. These codes analysed with data from the LA survey and WFM reports have been grouped into nine themes presented in four empirical chapters. Each theme, for example, Surveillance, Professional Development, Leadership and Policy draw on the data derived from the different methods of data gathering and analysis. My research data evidences that discrimination on an individual, institutional and structural level is still experienced in the leadership of children’s learning. The extent of this discrimination can only be explained in that it is designed. Policies that further deregulate and fragment the provision of education through schools and allied support structures only exacerbate this situation and therefore maintain White male supremacy in the leadership of children’s learning. Christopher Guy Vieler-Porter, The under-representation of black and minority ethnic educators in education: chance, coincidence or design?, Published November 2, 2020 by Routledge https://www.routledge.com/978100305143

Subcritical co-solvents extraction of lipid from wet microalgae pastes of Nannochloropsis sp

In this paper subcritical co-solvents extraction (SCE) of algal lipid from wet pastes of Nannochloropsis sp. is examined. The influences of five operating parameters including the ratio between ethanol to hexane, the ratio of mixed solvents to algal biomass (dry weight), extraction temperature, pressure, and time were investigated. The determined optimum extraction conditions were 3:1 (hexane to ethanol ratio), 10:1 ratio (co-solvents to microalgae (dry weight) ratio), 90°C, 1.4 MPa, and 50 min, which could produce 88% recovery rate of the total lipids. In addition, electron micrographs of transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were conducted to show that the algal cell presented shrunken, collapsed with some wrinkles and microholes after SCE extraction. The main composition of total lipids extracted under the optimum conditions was TAG which represented more than 80%. And the fatty acid profile of triglycerides revealed that C16:0 (35.67 ± 0.2%), C18:1 (26.84 ± 0.044%) and C16:1 (25.96 ± 0.011%) were dominant

Conservation of core complex subunits shaped the structure and function of photosystem I in the secondary endosymbiont alga Nannochloropsis gaditana

Photosystem I (PSI) is a pigment protein complex catalyzing the light-driven electron transport from plastocyanin to ferredoxin in oxygenic photosynthetic organisms. Several PSI subunits are highly conserved in cyanobacteria, algae and plants, whereas others are distributed differentially in the various organisms. Here we characterized the structural and functional properties of PSI purified from the heterokont alga Nannochloropsis gaditana, showing that it is organized as a supercomplex including a core complex and an outer antenna, as in plants and other eukaryotic algae. Differently from all known organisms, the N. gaditana PSI supercomplex contains five peripheral antenna proteins, identified by proteome analysis as type-R light-harvesting complexes (LHCr4-8). Two antenna subunits are bound in a conserved position, as in PSI in plants, whereas three additional antennae are associated with the core on the other side. This peculiar antenna association correlates with the presence of PsaF/J and the absence of PsaH, G and K in the N. gaditana genome and proteome. Excitation energy transfer in the supercomplex is highly efficient, leading to a very high trapping efficiency as observed in all other PSI eukaryotes, showing that although the supramolecular organization of PSI changed during evolution, fundamental functional properties such as trapping efficiency were maintained

Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga \u3ci\u3eNannochloropsis oceanica\u3c/i\u3e CCMP1779

Unicellular marine algae have promise for providing sustainable and scalable biofuel feedstocks, although no single species has emerged as a preferred organism. Moreover, adequate molecular and genetic resources prerequisite for the rational engineering of marine algal feedstocks are lacking for most candidate species. Heterokonts of the genus Nannochloropsis naturally have high cellular oil content and are already in use for industrial production of high-value lipid products. First success in applying reverse genetics by targeted gene replacement makes Nannochloropsis oceanica an attractive model to investigate the cell and molecular biology and biochemistry of this fascinating organism group. Here we present the assembly of the 28.7 Mb genome of N. oceanica CCMP1779. RNA sequencing data from nitrogen-replete and nitrogendepleted growth conditions support a total of 11,973 genes, of which in addition to automatic annotation some were manually inspected to predict the biochemical repertoire for this organism. Among others, more than 100 genes putatively related to lipid metabolism, 114 predicted transcription factors, and 109 transcriptional regulators were annotated. Comparison of the N. oceanica CCMP1779 gene repertoire with the recently published N. gaditana genome identified 2,649 genes likely specific to N. oceanica CCMP1779. Many of these N. oceanica–specific genes have putative orthologs in other species or are supported by transcriptional evidence. However, because similarity-based annotations are limited, functions of most of these species-specific genes remain unknown. Aside from the genome sequence and its analysis, protocols for the transformation of N. oceanica CCMP1779 are provided. The availability of genomic and transcriptomic data for Nannochloropsis oceanica CCMP1779, along with efficient transformation protocols, provides a blueprint for future detailed gene functional analysis and genetic engineering of Nannochloropsis species by a growing academic community focused on this genus

The Effect of Diel Temperature and Light Cycles on the Growth of Nannochloropsis oculata in a Photobioreactor Matrix

A matrix of photobioreactors integrated with metabolic sensors was used to examine the combined impact of light and temperature variations on the growth and physiology of the biofuel candidate microalgal species Nannochloropsis oculata. The experiments were performed with algal cultures maintained at a constant 20u C versus a 15°C to 25°C diel temperature cycle, where light intensity also followed a diel cycle with a maximum irradiance of 1920 μmol photons m-2 s-1. No differences in algal growth (Chlorophyll a) were found between the two environmental regimes; however, the metabolic processes responded differently throughout the day to the change in environmental conditions. The variable temperature treatment resulted in greater damage to photosystem II due to the combined effect of strong light and high temperature. Cellular functions responded differently to conditions before midday as opposed to the afternoon, leading to strong hysteresis in dissolved oxygen concentration, quantum yield of photosystem II and net photosynthesis. Overnight metabolism performed differently, probably as a result of the temperature impact on respiration. Our photobioreactor matrix has produced novel insights into the physiological response of Nannochloropsis oculata to simulated environmental conditions. This information can be used to predict the effectiveness of deploying Nannochloropsis oculata in similar field conditions for commercial biofuel production. © 2014 Tamburic et al

Microplate assay for quantitation of neutral lipids in extracts from microalgae

Lipid quantitation is widespread in the algae literature, but popular methods such as gravimetry, gas chromatography and mass spectrometry (GC-MS), and Nile red cell staining suffer drawbacks, including poor quantitation of neutral lipids, expensive equipment, and variable results among algae species, respectively. A high-throughput microplate assay was developed that uses Nile red dye to quantify neutral lipids that have been extracted from algae cells. Because the algal extracts contained pigments that quenched Nile red fluorescence, a mild bleach solution was used to destroy pigments, resulting in a nearly linear response for lipid quantities in the range of 0.75 to 40 μg. Corn oil was used as a standard for quantitation, although other vegetable oils displayed a similar response. The assay was tested on lipids extracted from three species of Chlorella and resulted in close agreement with triacylglycerol (TAG) levels determined by thin layer chromatography. The assay was found to more accurately measure algal lipids conducive to biodiesel production and nutrition applications than the widely used gravimetric assay. Assay response was also consistent among different species, in contrast to Nile red cell staining procedures