22 research outputs found
Widespread Endogenization of Genome Sequences of Non-Retroviral RNA Viruses into Plant Genomes
Non-retroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants. Here we report similarly endogenized NRVSs derived from plus-, negative-, and double-stranded RNA viruses in plant chromosomes. These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were subsequently detected by direct molecular analyses of plant DNAs. The most widespread NRVSs were related to the coat protein (CP) genes of the family Partitiviridae which have bisegmented dsRNA genomes, and included plant- and fungus-infecting members. The CP of a novel fungal virus (Rosellinia necatrix partitivirus 2, RnPV2) had the greatest sequence similarity to Arabidopsis thaliana ILR2, which is thought to regulate the activities of the phytohormone auxin, indole-3-acetic acid (IAA). Furthermore, partitivirus CP-like sequences much more closely related to plant partitiviruses than to RnPV2 were identified in a wide range of plant species. In addition, the nucleocapsid protein genes of cytorhabdoviruses and varicosaviruses were found in species of over 9 plant families, including Brassicaceae and Solanaceae. A replicase-like sequence of a betaflexivirus was identified in the cucumber genome. The pattern of occurrence of NRVSs and the phylogenetic analyses of NRVSs and related viruses indicate that multiple independent integrations into many plant lineages may have occurred. For example, one of the NRVSs was retained in Ar. thaliana but not in Ar. lyrata or other related Camelina species, whereas another NRVS displayed the reverse pattern. Our study has shown that single- and double-stranded RNA viral sequences are widespread in plant genomes, and shows the potential of genome integrated NRVSs to contribute to resolve unclear phylogenetic relationships of plant species
A survey of green plant tRNA 3'-end processing enzyme tRNase Zs, homologs of the candidate prostate cancer susceptibility protein ELAC2
<p>Abstract</p> <p>Background</p> <p>tRNase Z removes the 3'-trailer sequences from precursor tRNAs, which is an essential step preceding the addition of the CCA sequence. tRNase Z exists in the short (tRNase Z<sup>S</sup>) and long (tRNase Z<sup>L</sup>) forms. Based on the sequence characteristics, they can be divided into two major types: bacterial-type tRNase Z<sup>S </sup>and eukaryotic-type tRNase Z<sup>L</sup>, and one minor type, <it>Thermotoga maritima </it>(TM)-type tRNase Z<sup>S</sup>. The number of tRNase Zs is highly variable, with the largest number being identified experimentally in the flowering plant <it>Arabidopsis thaliana</it>. It is unknown whether multiple tRNase Zs found in <it>A. thaliana </it>is common to the plant kingdom. Also unknown is the extent of sequence and structural conservation among tRNase Zs from the plant kingdom.</p> <p>Results</p> <p>We report the identification and analysis of candidate tRNase Zs in 27 fully sequenced genomes of green plants, the great majority of which are flowering plants. It appears that green plants contain multiple distinct tRNase Zs predicted to reside in different subcellular compartments. Furthermore, while the bacterial-type tRNase Z<sup>S</sup>s are present only in basal land plants and green algae, the TM-type tRNase Z<sup>S</sup>s are widespread in green plants. The protein sequences of the TM-type tRNase Z<sup>S</sup>s identified in green plants are similar to those of the bacterial-type tRNase Z<sup>S</sup>s but have distinct features, including the TM-type flexible arm, the variant catalytic HEAT and HST motifs, and a lack of the PxKxRN motif involved in CCA anti-determination (inhibition of tRNase Z activity by CCA), which prevents tRNase Z cleavage of mature tRNAs. Examination of flowering plant chloroplast tRNA genes reveals that many of these genes encode partial CCA sequences. Based on our results and previous studies, we predict that the plant TM-type tRNase Z<sup>S</sup>s may not recognize the CCA sequence as an anti-determinant.</p> <p>Conclusions</p> <p>Our findings substantially expand the current repertoire of the TM-type tRNase Z<sup>S</sup>s and hint at the possibility that these proteins may have been selected for their ability to process chloroplast pre-tRNAs with whole or partial CCA sequences. Our results also support the coevolution of tRNase Zs and tRNA 3'-trailer sequences in plants.</p
Unravelling the genome of Holy basil: an “incomparable” “elixir of life” of traditional Indian medicine
Potential of pyrolysis of spacecraft solid waste for water recovery and plant-growth media production
La peau du bébé (formulation et développement de produits d'hygiène et de soin)
Les soins apportés au nouveau-né et au nourrisson nécessitent le respect de règles spécifiques, ainsi que l'utilisation de produit d'une extrême tolérance, du fait de l'immaturité cutanée et des particularités de l'environnement. Après avoir rappelé la structure et la physiologie de la peau de l'adulte, nous étudierons les spécificités de la peau du nourrisson. Puis nous évoquerons les différents types et formes de produits dermo-cosmétiques infantiles existants actuellement sur le marché. Et dans une dernière partie, nous traiterons des exigences imposées au niveau industriel pour le développement des produits dermo-cosmétiques à visée pédiatrique.TOULOUSE3-BU Santé-Centrale (315552105) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF