Tetra­aqua­bis(2-oxo-1,2-dihydro­quinoline-4-carboxyl­ato-κO 4)nickel(II)

In the title compound, [Ni(C10H6NO3)2(H2O)4], the central NiII atom is located on an inversion center and coordinated in a slightly distorted octa­hedral geometry by two O atoms from two 2-oxo-1,2-dihydro­quinoline-4-carboxyl­ate ligands and four water mol­ecules, all of which act as monodentate ligands. The crystal structure features an extensive network of inter­molecular hydrogen-bonding inter­actions (O—H⋯O and N—H⋯O) and offset face-to-face π–π stacking inter­actions [centroid–centroid distances = 3.525 (3) and 3.281 (5) Å]

Phase-resolved gamma-ray spectroscopy of the Crab pulsar observed by POLAR

The POLAR detector is a space based Gamma-Ray Burst (GRB) polarimeter sensitive in the 15-500 keV energy range. Apart from its main scientific goal as a Gamma-Ray Burst polarimeter it is also able to detect photons from pulsars in orbit. By using the six-months in-orbit observation data, significant pulsation from the PSR B0531+21 (Crab pulsar) was obtained. In this work, we present the precise timing analysis of the Crab pulsar, together with a phase-resolved spectroscopic study using a joint-fitting method adapted for wide field of view instruments like POLAR. By using single power law fitting over the pulsed phase, we obtained spectral indices ranging from 1.718 to 2.315, and confirmed the spectral evolution in a reverse S shape which is homogenous with results from other missions over broadband. We will also show, based on the POLAR in-orbit performance and Geant4 Monte-Carlo simulation, the inferred capabilities of POLAR-2, the proposed follow-up mission of POLAR on board the China Space Station (CSS), for pulsars studies.Comment: 15 pages, 9 figures, 2 tables, accepted for publication in Journal of High Energy Astrophysic

Genotyping and biofilm formation of Mycoplasma hyopneumoniae and their association with virulence

Mycoplasma hyopneumoniae, the causative agent of swine respiratory disease, demonstrates differences in virulence. However, factors associated with this variation remain unknown. We herein evaluated the association between differences in virulence and genotypes as well as phenotype (i.e., biofilm formation ability). Strains 168 L, RM48, XLW-2, and J show low virulence and strains 232, 7448, 7422, 168, NJ, and LH show high virulence, as determined through animal challenge experiments, complemented with in vitro tracheal mucosa infection tests. These 10 strains with known virulence were then subjected to classification via multilocus sequence typing (MLST) with three housekeeping genes, P146-based genotyping, and multilocus variable-number tandem-repeat analysis (MLVA) of 13 loci. MLST and P146-based genotyping identified 168, 168 L, NJ, and RM48 as the same type and clustered them in a single branch. MLVA assigned a different sequence type to each strain. Simpson’s index of diversity indicates a higher discriminatory ability for MLVA. However, no statistically significant correlation was found between genotypes and virulence. Furthermore, we investigated the correlation between virulence and biofilm formation ability. The strains showing high virulence demonstrate strong biofilm formation ability, while attenuated strains show low biofilm formation ability. Pearson correlation analysis revealed a significant positive correlation between biofilm formation ability and virulence. To conclude, there was no association between virulence and our genotyping data, but virulence was found to be significantly associated with the biofilm formation ability of M. hyopneumoniae

Experimental study on LBL beams

Six specimens were made and tested to study the mechanical properties of LBL beams. The mean ultimate loading value is 68.39 MPa with a standard deviation of 6.37 MPa, giving a characteristic strength (expected to be exceeded by 95% of specimens) of 57.91 MPa, and the mean ultimate deflection is 53.3 mm with a standard deviation of 5.5 mm, giving the characteristic elastic modulus of 44.3 mm. The mean ultimate bending moment is 20.18 kN.m with a standard deviation of 1.88 kN.m, giving the characteristic elastic modulus of 17.08 kN.m. The mean elastic modulus is 9688 MPa with a standard deviation of 1765 MPa, giving the characteristic elastic modulus of 6785 MPa, and the mean modulus of rupture is 93.3 MPa with a standard deviation of 8.6 MPa, giving the characteristic elastic modulus of 79.2 MPa. The strain across the cross-section for all LBL beams is basically linear throughout the loading process, following standard beam theory

Online state of charge estimation for the aerial lithium-ion battery packs based on the improved extended Kalman filter method.

An effective method to estimate the integrated state of charge (SOC) value for the lithium-ion battery (LIB) pack is proposed, because of its capacity state estimation needs in the high-power energy supply applications, which is calculated by using the improved extended Kalman filter (EKF) method together with the one order equivalent circuit model (ECM) to evaluate its remaining available power state. It is realized by the comprehensive estimation together with the discharging and charging maintenance (DCM) process, implying an accurate remaining power estimation with low computational calculation demand. The battery maintenance and test system (BMTS) equipment for the aerial LIB pack is developed, which is based on the proposed SOC estimation method. Experimental results show that, it can estimate SOC value of the LIB pack effectively. The BMTS equipment has the advantages of high detection accuracy and stability and can guarantee its power-supply reliability. The SOC estimation method is realized on it, the results of which are compared with the conventional SOC estimation method. The estimation has been done with an accuracy rate of 95% and has an absolute root mean square error (RMSE) of 1.33% and an absolute maximum error of 4.95%. This novel method can provide reliable technical support for the LIB power supply application, which plays a core role in promoting its power supply applications

Suppress HBV by therapeutic vaccine

乙肝预防性疫苗显著减少了乙肝新发感染，但目前全球仍有约2.5亿慢性乙肝感染者，若未得到有效治疗，可能发展为肝癌、肝硬化等终末期肝病并导致死亡。夏宁邵教授团队研究发展了一种新型的B细胞表位嵌合型类病毒颗粒乙肝治疗性疫苗（治疗性蛋白），在多种模型中证实了其对慢性乙肝感染的治疗潜力，为研发治疗慢性乙肝的原创药物提供了新思路。 我校博士后张天英、博士生郭雪染和博士生巫洋涛为该论文共同第一作者，夏宁邵教授、袁权副教授、张军教授为该论文的共同通讯作者。【Abstract】Objective: This study aimed to develop a novel therapeutic vaccine based on a unique B cell epitope and investigate its therapeutic potential against chronic hepatitis B (CHB) in animal models. Methods: A series of peptides and carrier proteins were evaluated in HBV-tolerant mice to obtain an optimized therapeutic molecule. The immunogenicity,therapeutic efficacy and mechanism of the candidate were investigated systematically. Results: Among the HBsAg-aa119-125-containing peptides evaluated in this study, HBsAg-aa113-135 (SEQ13) exhibited the most striking therapeutic effects. A novel immuno-enhanced virus-like particle carrier (CR-T3) derived from the roundleaf bat HBV core antigen (RBHBcAg) was created and used to display SEQ13, forming candidate molecule CR-T3-SEQ13. Multiple copies of SEQ13 displayed on the surface of this particulate antigen promote the induction of a potent anti-HBs antibody response in mice, rabbits and cynomolgus monkeys. Sera and purified polyclonal IgG from the immunized animals neutralized HBV infection in vitro and mediated efficient HBV/HBsAg clearance in the mice. CR-T3-SEQ13-based vaccination induced long-term suppression of HBsAg and HBV DNA in HBV transgenic mice and eradicated the virus completely in hydrodynamic-based HBV carrier mice. The suppressive effects on HBsAg were strongly correlated with the anti-HBs level after vaccination, suggesting that the main mechanism of CR-T3-SEQ13 vaccination therapy was the induction of a SEQ13-specific antibody response that mediated HBV/HBsAg clearance. Conclusions: The novel particulate protein CR-T3-SEQ13 suppressed HBsAg effectively through induction of a humoral immune response in HBV-tolerant mice. This B cell epitope-based therapeutic vaccine may provide a novel immunotherapeutic agent against chronic HBV infection in humans.This work was supported by the National Scientific and Technological Major project (2017ZX10202203-001), the National Natural Science Foundation of China (31730029, 81672023, 81871316 and 81702006) and the Xiamen University President Fund Project (20720160063). 该研究获得了“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项、国家自然科学基金等资助

Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection

An ultra-high sensitivity label-free optical fiber biosensor for inactivated Staphylococcus aureus (S. aureus) detection is proposed and investigated in this study, with additional advantages of robust and stability compared to traditional tapered fiber structure. The proposed fiber biosensor is based on a tapered singlemode- no core-singlemode fiber coupler (SNSFC) structure, where the no core fiber was tapered to small diameter (taper-waist diameter of about 10 µm) and functionalized with the pig immunoglobulin G (IgG) antibody for detection of S. aureus. The measured maximum wavelength shift of the sensor for an S. aureus concentration of 7 × 101 CFU/ml (colony forming unit per milliliter) is 2.04 nm, which is equivalent to a limit of detection (LOD) of 3.1 CFU/ml (a highest LOD reported so far for optical fiber biosensors), considering the maximum wavelength variation of the sensor in phosphate buffered saline (PBS) is ±0.03 nm over 40 minutes, where 3 times of maximum wavelength variation (3 × 0.03 = 0.09 nm) is defined as measurement limit. The response time of the developed fiber sensor is less than 30 minutes. The ultra-sensitive biosensor has potential to be widely applied to various areas such as disease, medical diagnostics and food safety inspection

Meta-Analysis Results on the Association Between TP53 Codon 72 Polymorphism With the Susceptibility to Oral Cancer

Objectives: TP53 is an important tumor suppressor gene to maintain genomic integrity, and its mutations increase the susceptibility to oral carcinoma. Previous published studies have reported the relation of TP53 codon 72 polymorphism with the risk of oral carcinoma, but the results remain controversial and inconclusive.Methods: We therefore utilized meta-analysis based on a comprehensive search in PubMed, EMBASE, and Google of Scholar databases up to August 19, 2017.Results: Total 3,525 cases and 3,712 controls from 21 case-control studies were selected. We found no significant association between TP53 codon 72 polymorphism and oral carcinoma susceptibility in all genetic contrast models, including subgroup analysis based on control source and ethnicity. Furthermore, TP53 codon 72 polymorphism was not significant associated with oral carcinoma susceptibility in tobacco or alcohol use, and HPV infection status. Our results were confirmed by sensitivity analysis and no publication bias was found.Conclusions: Taken together, our data indicate that TP53 codon 72 polymorphism is not associated with the susceptibility to oral carcinoma