Hallazgos especiales del sitio Casa de Piedra de Ortega : provincia de Río Negro

Presentamos el análisis de los hallazgos especiales del sitio Casa de Piedra de Ortega, provincia de Río Negro. El conjunto abarca cuentas de vidrio, hueso, piedra y valva; valvas con decoración y/o adherencias, recortes de hueso cuadrangulares, un fragmento óseo decorado y placas de piedra y cáscaras de huevo de ñandú grabadas. Al estudio microscópico de los materiales se sumó la observación con lupa binocular de bajos aumentos, para conocer las técnicas de manufactura y los procesos de modificación posteriores a la confección, y la información proveniente de documentos escritos. Finalmente, realizamos consideraciones acerca del valor simbólico de estos objetos.Fil: Fernández, Mabel M.. Universidad Nacional de LujánFil: Ramos, Mariano S. . Consejo Nacional de Investigaciones Científicas y Técnica

Los artefactos líticos del Alero Carriqueo : informe de avance 2010

El denominado alero Carriqueo se ubica en Nordpatagonia, en la cuenca del río Limay. El actual sitio arqueológico fue recurrentemente habitado durante un amplio período. Hasta el presente el registro arqueológico, abundante y variado, se puede vincular al período ceramolítico de la región. En este trabajo brindamos información acerca de los resultados obtenidos luego del estudio morfológico-descriptivo de una muestra de artefactos líticos que hemos dividido en débitage lato sensu por un lado (débitage stricto sensu más las matrices bifaciales y los núcleos) y por el otro, en instrumentos. Todos proceden de las excavaciones realizadas en el sitio hasta la actualidad.The Carriqueo Nordpatagonia rock shelter is located in the Lima)/ River basin. The current archaeological site was occupied repeatedly over an extended period. So far the archaeological record, generous and varied, can be linked to the region ceramolítico period. In this paper we provide information about the results after morphologicaldescriptive study of a sample of lithic debitage we divided into sensu lato on one side (sensu stricto debitage more bifacial matrix and cores) and the other in instruments. All come from the excavations at the site until today.Fil: Palacios, Oscar M. . Universidad de Buenos AiresFil: Ramos, Mariano S. . Universidad Nacional de Lujá

El Taller lítico LY 041-La Divisoria (Departamento Pilcaniyeu, Provincia de Río Negro)

En el taller lítico LY 041-La Divisoria, situado en el sudoeste de la estepa rionegrina, se prepararon artefactos bifaciales en rocas dacíticas procedentes de la cercana cantera Paso Limay. Habría sido un satélite del alero Carriqueo, un pequeño sitio de vivienda muy próximo, del cual se habrían segregado algunas tareas de talla, que generan muchos desechos potencialmente molestos o peligrosos. Además, desde el sitio se tiene buena visibilidad del paisaje. Subsidiariamente, en LY 041-La Divisoria se habrían realizado algunas tareas de procesamiento de materiales orgánicos. Este sitio corrobora que para los cazadores-recolectores de la comarca, la cantera Paso Limay tuvo un papel central en el aprovisionamiento de roca para puntas de proyectil.LY 041-La Divisoria is a lithic workshop in the southwestern steppe of the Río Negro Province, Argentina, where dacite brought from the Paso Limay quarry was reduced bifacially. This workshop would have been a satellite of close-by Carriqueo rockshelter, a small living cite from which certain tasks were sometimes shifted because they generated encumbering refuse. LY 041-La Divisoria provided also a good view of the environment. Subsidiarily, some processing of organic materials was carried out. LY 041-La Divisoria corroborates that for the local hunter-gatherers, the Paso Limay quarry was of major importance for the provisioning of raw material for projectile-points.Fil: Crivelli Montero, Eduardo A. . Universidad de Buenos AiresFil: Ramos, Mariano S. . Universidad Nacional de Lujá

Trace fossils from the Upper Cretaceous Capacete Formation, Sanfranciscana Basin, Central Brazil

The Upper Cretaceous Capacete Formation, from the intracratonic Sanfranciscana Basin, central Brazil, consists of epiclastic sediments with a significant aeolian contribution. This unit has been traditionally interpreted as deposited by alluvial fans distally grading to braided rivers in an arid- to semiarid climate within the central part of southwestern Gondwana. Locally, some expositions with wide lateral extension show architectural ele-ments indicative of deposition in meandering rivers, such as floodplain fines (FF) interbedded with crevasse splay (CR), lateral-accretion macroforms (LA), and channels (CH). Bioturbation within the meandering fluvial deposits is commonly associated with the floodplain element, mainly occurring in fine-grained sandstone and mudstone. The lateral-accretion elements and channel deposits are barren of trace fossils. Sandstone with climbing ripples (Sr) from the element CR show low bioturbation of meniscate trace fossils assigned to Taenidium, Beaconites, and Scoyenia ichnogenera. Simple vertical and horizontal burrows identified as Skolithos and Palaeophycus occur associated with Taenidium and Camborygma in sandstone facies (Sr, St and Sh), as well as rhizoliths, representing the Scoyenia ichnofacies. The predominance of trace fossils related to the FF and CR architectural elements attests to the colonization mostly by insects in episodic and vegetated flood plains of the meandering river system from the Capacete Formation. Thus, despite the primarily arid to semiarid conditions interpreted for the inner parts of the newly formed South American continent during the Upper Cretaceous, our new data support deposition under humid conditions in some intervals of the Capacete Formation from the Sanfranciscana Basin

Arterial stiffness is associated with adipokine dysregulation in non-hypertensive obese mice

This is the peer reviewed version of the following article: Vascular Pharmacology 77 (2016): 38-47, which has been published in final form at http://dx.doi.org/10.1016/j.vph.2015.05.012The aim of this study was to characterize alterations in vascular structure and mechanics in murine mesenteric arteries from obese non-hypertensive mice, as well as their relationship with adipokines. Four-week old C57BL/6J male mice were assigned either to a control (C, 10% kcal from fat) or a high-fat diet (HFD, 45% kcal from fat) for 32 weeks. HFD animals weighed 30% more than controls (p < 0.001), exhibited similar blood pressure, increased leptin, insulin and superoxide anion (O2radical dot−) levels, and reduced adiponectin levels and nitric oxide (NO) bioavailability. Arterial structure showed an outward remodeling with an increase in total number of both adventitial and smooth muscle cells in HFD. Moreover, HFD mice exhibited an increased arterial stiffness assessed by β-values (C = 2.4 ± 0.5 vs HFD = 5.3 ± 0.8; p < 0.05) and aortic pulse wave velocity (PWV, C = 3.4 ± 0.1 vs HFD = 3.9 ± 0.1; p < 0.05). β-Values and PWV positively correlated with leptin, insulin or O2radical dot− levels, whereas they negatively correlated with adiponectin levels and NO bioavailability (p < 0.01). A reduction in fenestrae number together with an increase in type-I collagen amount (p < 0.05) were observed in HFD. These data demonstrate that HFD accounts for the development of vascular remodeling and arterial stiffness associated with adipokine dysregulation and oxidative stress, independently of hypertension developmentThis work was supported by grants from Ministerio de Ciencia e Investigación (BFU2011-25303), Ministerio de Economía y Competitividad (SAF2009-09714, SAF2011-25303, BFU2012-35353), Grupos Universidad Complutense de Madrid (UCM; GR-921641), Fundación Universitaria CEU-San Pablo, Fundación Mutua Madrileña and Sociedad para el Estudio de la Salud Cardiometabólica (SESCAMET). MGO is recipient of a Ministerio de Educación y Ciencia fellowshi

Analysis of fluid velocity inside an agricultural sprayer using generalized linear mixed models

The fluid velocity inside the tank of agricultural sprayers is an indicator of the quality of the mixture. This study aims to formulate the best generalized linear mixed model to infer the fluid velocity inside a tank under specific operational parameters of the agitation system, such as liquid level, circuit pressures, and number of active nozzles. A complex model was developed that included operational parameters as fixed eects (FE) and the section of the tank as the random eect. The goodness of fit of the model was evaluated by considering the lowest values of Akaike''s information criteria and Bayesian information criterion, and by estimating the residual variance. The gamma distribution and log-link function enhanced the goodness of fit of the best model. The Toeplitz structure was chosen as the structure of the covariance matrix. SPSS and SAS software were used to compute the model. The analysis showed that the greatest influence on the fluid velocity was exerted by the liquid level in the tank, followed by the circuit pressure and, finally, the number of active nozzles. The development presented here could serve as a guide for formulating models to evaluate the eciency of the agitation system of agricultural sprayers

High Quality Long-Term CD4+ and CD8+ Effector Memory Populations Stimulated by DNA-LACK/MVA-LACK Regimen in Leishmania major BALB/c Model of Infection

Heterologous vaccination based on priming with a plasmid DNA vector and boosting with an attenuated vaccinia virus MVA recombinant, with both vectors expressing the Leishmania infantum LACK antigen (DNA-LACK and MVA-LACK), has shown efficacy conferring protection in murine and canine models against cutaneus and visceral leishmaniasis, but the immune parameters of protection remain ill defined. Here we performed by flow cytometry an in depth analysis of the T cell populations induced in BALB/c mice during the vaccination protocol DNA-LACK/MVA-LACK, as well as after challenge with L. major parasites. In the adaptive response, there is a polyfunctional CD4+ and CD8+ T cell activation against LACK antigen. At the memory phase the heterologous vaccination induces high quality LACK-specific long-term CD4+ and CD8+ effector memory cells. After parasite challenge, there is a moderate boosting of LACK-specific CD4+ and CD8+ T cells. Anti-vector responses were largely CD8+-mediated. The immune parameters induced against LACK and triggered by the combined vaccination DNA/MVA protocol, like polyfunctionality of CD4+ and CD8+ T cells with an effector phenotype, could be relevant in protection against leishmaniasis

Snake Venom Disintegrins and Cell Migration

Cell migration is a key process for the defense of pluricellular organisms against pathogens, and it involves a set of surface receptors acting in an ordered fashion to contribute directionality to the movement. Among these receptors are the integrins, which connect the cell cytoskeleton to the extracellular matrix components, thus playing a central role in cell migration. Integrin clustering at focal adhesions drives actin polymerization along the cell leading edge, resulting in polarity of cell movement. Therefore, small integrin-binding proteins such as the snake venom disintegrins that inhibit integrin-mediated cell adhesion are expected to inhibit cell migration. Here we review the current knowledge on disintegrin and disintegrin-like protein effects on cell migration and their potential use as pharmacological tools in anti-inflammatory therapy as well as in inhibition of metastatic invasion

Immunogenicity and efficacy of a novel multi-patch SARS-CoV-2/COVID-19 vaccine candidate

23 Pág.While there has been considerable progress in the development of vaccines against SARS-CoV-2, largely based on the S (spike) protein of the virus, less progress has been made with vaccines delivering different viral antigens with cross-reactive potential.This research was supported by La Caixa Banking Foundation grant CF01-00008, Spanish Ministry of Science and Innovation (MCIN)/Spanish Research Agency grant PID2020-117425RB-C22, Fondo COVID-19 grant COV20/00151 (Spanish Health Ministry and Instituto de Salud Carlos III), CSIC grant 2020E84 and Ferrovial donations (to ME). AcknowledgmentsPeer reviewe