Plant mucilage increases pull-out resistance of root analogues from soil

ACKNOWLEDGEMENTS We wish to thank Dr. Muhammad Naveed and Dr. Ewan Oleghe for guidance on experimental approaches. This research was supported by the Natural Science Foundation of Shanxi Province of China (20210302123105) and the Shanxi Scholarship Council of China (2020-054).Peer reviewe

Long-term field pH manipulation influence on microbial activity, water repellency and physical properties of soil

Studies across multiple soils find increasing pH decreases water repellency. In this study, water repellency and a range of other soil physical properties of bulk soils, aggregates and intact specimens were measured on a long-term pH field experiment on a single sandy loam soil under a ley-arable crop rotation, with soil pH adjustments occurring annually by adding FeSO4 or CaCO3, to lower or raise the pH, respectively. Crop impacts were investigated by comparing 3rd year grass-white clover to spring oats, at the beginning (May) and end (September) of the growing season to allow soil structure comparisons. As in previous research, increased CO2 microbial respiration (p 55 years of chemical additions to amend soil pH

Plant exudates may stabilize or weaken soil depending on species, origin and time

We hypothesized that plant exudates could either gel or disperse soil depending on their chemical characteristics. Barley (Hordeum vulgare L. cv. Optic) and maize (Zea mays L.cv. Freya) root exudates were collected using an aerated hydroponic method and compared to chia (Salvia hispanica L.) seed exudate, a commonly used root exudate analogue. Sandy loam soil passed through a 500-μm mesh was treated with each exudate at a concentrationof 4.6 mg exudate g-1 dry soil. Two sets of soil samples were prepared, One set of treated soil samples was maintained at 4oC to suppress microbial processes. To characterize the effect of decomposition, the second set of samples was incubated at 16C for 2 weeks at – 30 kPa matric potential. Gas chromatography–mass spectrometry (GC–MS) analysis of the exudates found that barley had the largest organic acid content and chia the largest content of sugars (polysaccharide-derived or free), and maize was in between barley and chia. Yield stress of amended soil samples was measured by an oscillatory strain sweep test with a cone plate rheometer. When microbial decomposition was suppressed at 4oC, yield stress increased 20-fold for chia seed exudate and two-fold for maize root exudate compared to the control, whereas for barley root exudate it decreased to half. The yield stress after 2 weeks of incubation compared to soil with suppressed microbial decomposition increased by 85% for barley root exudate, but for chia and maize it decreased to by 87% and 54%, respectively. Barley root exudation might therefore disperse soil and this could facilitate nutrient release. The maize root and chia seed exudates gelled soil, which could create a more stable soil structure around roots or seeds

Extent and persistence of soil water repellency induced by pines in different geographic regions

This work was supported by the Slovak Scientific Grant Agency VEGA Project Nos. 2/0054/14 and 2/0009/2015, the Slovak Research and Development Agency Project No. APVV-15-0160, and it results from the project implementation of the “Centre of excellence for integrated flood protection of land” (ITMS 26240120004).Peer reviewedPublisher PD

Imaging microstructure of the barley rhizosphere:particle packing and root hair influences

Soil adjacent to roots has distinct structural and physical properties from bulk soil, affecting water and solute acquisition by plants. Detailed knowledge on how root activity and traits such as root hairs affect the three-dimensional pore structure at a fine scale is scarce and often contradictory. Roots of hairless barley (Hordeum vulgare L. cv Optic) mutant (NRH) and its wildtype (WT) parent were grown in tubes of sieved (&lt;250 μm) sandy loam soil under two different water regimes. The tubes were scanned by synchrotron-based X-ray computed tomography to visualise pore structure at the soil–root interface. Pore volume fraction and pore size distribution were analysed vs distance within 1 mm of the root surface. Less dense packing of particles at the root surface was hypothesised to cause the observed increased pore volume fraction immediately next to the epidermis. The pore size distribution was narrower due to a decreased fraction of larger pores. There were no statistically significant differences in pore structure between genotypes or moisture conditions. A model is proposed that describes the variation in porosity near roots taking into account soil compaction and the surface effect at the root surface.</p

High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation

In this paper, we provide direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high resolution (~5 μm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare L.), with and without root hairs, were grown for 8 days in microcosms packed with sandy loam soil at 1.2 g cm-3 36 dry bulk density. Root hairs were visualised within air filled pore spaces, but not in the fine-textured soil regions. - We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (&gt; 5 μm) in the rhizosphere, as compared with the no-hair mutants. Both genotypes showed decreasing pore-space between 0.8 mm and 0.1 mm from the root surface. Interestingly the root-hair-bearing genotype had a significantly greater soil pore volume-fraction at the root-soil interface. - Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image based modelling

Interaction between contrasting rice genotypes and soil physical conditions induced by hydraulic stresses typical of alternate wetting and drying irrigation of soil

Background and aims: Alternate wetting and drying (AWD) saves water in paddy rice production but could influence soil physical conditions and root growth. This study investigated the interaction between contrasting rice genotypes, soil structure and mechanical impedance influenced by hydraulic stresses typical of AWD. Methods: Contrasting rice genotypes, IR64 and deeper- rooting Black Gora were grown in various soil conditions for 2 weeks. For the AWD treatments the soil was either maintained in a puddled state, equilibrated to −5 kPa (WET), or dried to −50 kPa and then rewetted at thewater potential of −5 kPa (DRY-WET). There was an additional manipulated macropore structure treatment, i.e. the soil was broken into aggregates, packed into cores and equilibrated to −5 kPa (REPACKED). A flooded treatment (puddled soil remained flooded until harvest) was set as a control (FLOODED). Soil bulk density, penetration resistance and X-ray Computed Tomography (CT) derived macropore structure were measured. Total root length, root surface area, root volume, average diameter, and tip number were determined by WinRhizo. Results: AWD induced formation of macropores and slightly increased soil mechanical impedance. The total root length of the AWD and REPACKED treatments were 1.7–2.2 and 3.5–4.2 times greater than that of the FLOODED treatment. There was no significant difference between WET and DRY-WET treatments. The differences between genotypes were minimal. Conclusions: AWD influenced soil physical properties and some root characteristics of rice seedlings, but drying soil initially to −50 kPa versus −5 kPa had no impact. Macropores formed intentionally from repacking caused a large change in root characteristics

Extent and persistence of soil water repellency induced by pines in different geographic regions

The extent (determined by the repellency indices RI and RIc) and persistence (determined by the water drop penetration time, WDPT) of soil water repellency (SWR) induced by pines were assessed in vastly different geographic regions. The actual SWR characteristics were estimated in situ in clay loam soil at Ciavolo, Italy (CiF), sandy soil at Culbin, United Kingdom (CuF), silty clay soil at Javea, Spain (JaF), and sandy soil at Sekule, Slovakia (SeF). For Culbin soil, the potential SWR characteristics were also determined after oven-drying at 60°C (CuD). For two of the three pine species considered, strong (Pinus pinaster at CiF) and severe (Pinus sylvestris at CuD and SeF) SWR conditions were observed. Pinus halepensis trees induced slight SWR at JaF site. RI and RIc increased in the order: JaF < CuF < CiF < CuD < SeF, reflecting nearly the same order of WDPT increase. A lognormal distribution fitted well to histograms of RIc data from CuF and JaF, whereas CiF, CuD and SeF had multimodal distributions. RI correlated closely with WDPT, which was used to develop a classification of RI that showed a robust statistical agreement with WDPT classification according to three different versions of Kappa coefficient

Dataset for High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation

Dataset supports: Koebernick, N.et al (2017). High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation. New Phytologist. </span