873 research outputs found
Structures from Powders: Polynuclear Hg(II) Complexes Containing the Flexible Bisimidazolylmethane Ligand
Ruthenium-Arene Complexes of Curcumin: X-Ray and Density Functional Theory Structure, Synthesis, and Spectroscopic Characterization, in Vitro Antitumor Activity, and DNA Docking Studies of (p-Cymene)Ru(curcuminato)chloro.
Statistical optimization of a culture medium for biomass and poly(3-hydroxybutyrate) production by a recombinant Escherichia coli strain using agroindustrial byproducts
A statistically based Plackett-Burman screening design identified milk whey and corn steep liquor concentrations as well as ionic strength (based on phosphate buffer concentration) as the three main independent components of the culture medium that significantly (p < 0.05) influenced biomass and poly(3-hydroxybutyrate) (PHB) production in recombinant cells of Escherichia coli. This strain carries a plasmid encoding phb genes from a natural isolate of Azotobacte sp. Response surface methodology, using a central composite rotatable design, demonstrated that the optimal concentrations of the three components, defined as those yielding maximal biomass and PHB production in shaken flasks, were 37.96 g deproteinated milk whey powder/l, 29.39 g corn steep liquor/l, and 23.76 g phosphates/l (r2 = 0.957). The model was validated by culturing the recombinant cells in medium containing these optimal concentrations, which yielded 9.41 g biomass/l and 6.12 g PHB/l in the culture broth. Similar amounts of PHB were obtained following batch fermentations in a bioreactor. These results show that PHB can be produced efficiently by culturing the recombinant strain in medium containing cheap carbon and nitrogen sources. [Int Microbiol 2005; 8(4):243-250
Effect of polyphenolic compounds on the proteolytic activities of constitutive and immuno-proteasomes
The effect of several polyphenols on the 20S proteasomes, both the constitutive and the LMP proteasomes, isolated from bovine tissues, has been investigated. Polyphenolic compounds show many biological activities such as antiviral, antibacterial, antifungal, anti-inflammatory, antimutagenic, and antiallergic activities.
However, the molecular mechanism underlying these effects has not been identified. It is well established that polyphenols possess inhibitory activities on several enzymes and among them the 20S proteasome. In the present work, the ChT-L, BrAAP, PGPH, and T-L activities of the isolated constitutive and immuno-proteasomes were assayed in order to get an overall information on the polyphenols binding to the complexes. The effects of the polyphenols on the proteasomal activities were analyzed, taking into account the different subunits composition of the two complexes. Furthermore the same activities were measured on whole extracts from cancer cells exposed to EGCG and gallic acid, evaluating, also, their antioxidant action under oxidative stress. EGCG and gallic acid are able to affect the 20S proteasomes functionality, depending on the complex subunit
composition and, in cell extracts, they behave both as antioxidants and proteasome effectors
20S proteasome mediated degradation of DHFR: implications in neurodegenerative disorders
The 20S proteasome is responsible for the degradation of protein substrates implicated in the onset and progression of neurodegenerative disorders, such as a-synuclein and tau protein. Here we show that the 20S proteasome isolated from bovine brain directly hydrolyzes, in vitro, the dihydrofolate reductase (DHFR), demonstrated to be involved in the pathogenesis of neurodegenerative diseases. Furthermore, the DHFR susceptibility to proteolysis is enhanced by oxidative conditions induced by peroxynitrite, mimicking the oxidative environment typical of these disorders. The results obtained suggest that the folate metabolism may be impaired by an increased degradation of DHFR, mediated by the 20S proteasome
Raman scattering reveals strong LO-phonon-hole-plasmon coupling in nominally undoped GaAsBi: optical determination of carrier concentration
We report room-temperature Raman scattering studies of nominally undoped (100) GaAs1−xBix epitaxial layers exhibiting Biinduced (p-type) longitudinal-optical-plasmon coupled (LOPC) modes for 0.018≤x≤0.048. Redshifts in the GaAs-like optical modes due to alloying are evaluated and are paralleled by strong damping of the LOPC. The relative integrated Raman intensities of LO(Γ) and LOPC ALO/ALOPC are characteristic of heavily doped p-GaAs, with a remarkable near total screening of the LO(Γ) phonon (ALO/ALOPC →0) for larger Bi concentrations. A method of spectral analysis is set out which yields estimates of hole concentrations in excess of 5 × 1017 cm−3 and correlates with the Bi molar fraction. These findings are in general agreement with recent electrical transport measurements performed on the alloy, and while the absolute size of the hole concentrations differ, likely origins for the discrepancy are discussed. We conclude that the damped LO-phonon-hole-plasmon coupling phenomena plays a dominant role in Raman scattering from unpassivated nominally undoped GaAsBi
Kinetic Inductance Detectors for the OLIMPO experiment: design and pre-flight characterization
We designed, fabricated, and characterized four arrays of horn--coupled,
lumped element kinetic inductance detectors (LEKIDs), optimized to work in the
spectral bands of the balloon-borne OLIMPO experiment. OLIMPO is a 2.6 m
aperture telescope, aimed at spectroscopic measurements of the
Sunyaev-Zel'dovich (SZ) effect. OLIMPO will also validate the LEKID technology
in a representative space environment. The corrected focal plane is filled with
diffraction limited horn-coupled KID arrays, with 19, 37, 23, 41 active pixels
respectively at 150, 250, 350, and 460GHz. Here we report on the full
electrical and optical characterization performed on these detector arrays
before the flight. In a dark laboratory cryostat, we measured the resonator
electrical parameters, such as the quality factors and the electrical
responsivities, at a base temperature of 300mK. The measured average
resonator s are 1.7, 7.0, 1.0, and
1.0 for the 150, 250, 350, and 460GHz arrays, respectively.
The average electrical phase responsivities on resonance are 1.4rad/pW,
1.5rad/pW, 2.1rad/pW, and 2.1rad/pW; the electrical noise
equivalent powers are 45, 160,
80, and 140, at 12 Hz. In the OLIMPO
cryostat, we measured the optical properties, such as the noise equivalent
temperatures (NET) and the spectral responses. The measured NETs are
, , ,
and , at 12 Hz; under 78, 88, 92, and 90 mK
Rayleigh-Jeans blackbody load changes respectively for the 150, 250, 350, and
460 GHz arrays. The spectral responses were characterized with the OLIMPO
differential Fourier transform spectrometer (DFTS) up to THz frequencies, with
a resolution of 1.8 GHz.Comment: Published on JCA
Herramientas para el seguimiento de procesos de interactividad mediatizados por facebook en educación superior
Esta ponencia presenta diversas herramientas para el seguimiento y evaluación de procesos de interactividad mediatizados por Facebook que pueden utilizarse para el análisis de propuestas educativas de nivel superior. A tal fin, fue necesario precisar y profundizar los conceptos de redes sociales y sus herramientas de análisis existentes. Enmarcados en la noción de Dispositivo Hipermedial Dinámico como una propuesta innovadora para construir aprendizajes significativos, abordamos dichos instrumentos tecnológicos con la clara finalidad de analizar su utilización y su capacidad real. Aunque Facebook tuvo su origen en un ambiente especÃficamente universitario, se evidencia que la mayorÃa de estas aplicaciones carecen de especificidad para un enfoque educativo y orientan su finalidad a contextos comerciales o de mercado. Sin embargo, existe un grupo reducido de estas cuyos resultados pueden aportar elementos de análisis, como asà también algunos desarrollos a nivel prototipo que podrÃan aportar soluciones originales.Eje: Workshop TecnologÃa informática aplicada en educación (WTIAE)Red de Universidades con Carreras en Informática (RedUNCI
Peculiarities of the hydrogenated In(AsN) alloy
The electronic properties of In(AsN) before and after post-growth sample irradiation with increasing doses of atomic hydrogen have been investigated by photoluminescence. The electron density increases in In(AsN) but not in N-free InAs, until a Fermi stabilization energy is established. A hydrogen ε+/− transition level just below the conduction band minimum accounts for the dependence of donor formation on N, in agreement with a recent theoretical report highlighting the peculiarity of InAs among III–V compounds. Raman scattering measurements indicate the formation of N–H complexes that are stable under thermal annealing up to ∼500 K. Finally, hydrogen does not passivate the electronic activity of N, thus leaving the band gap energy of In(AsN) unchanged, once more in stark contrast to what has been reported in other dilute nitride alloys
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