890 research outputs found
Generic mission planning concepts for space astronomy missions
The past two decades have seen the rapid development of space astronomy, both manned and unmanned, and the concurrent proliferation of the operational concepts and software that have been produced to support each individual project. Having been involved in four of these missions since the '70's and three yet to fly in the present decade, the authors believe it is time to step back and evaluate this body of experience from a macro-systems point of view to determine the potential for generic mission planning concepts that could be applied to future missions. This paper presents an organized evaluation of astronomy mission planning functions, functional flows, iteration cycles, replanning activities, and the requirements that drive individual concepts to specific solutions. The conclusions drawn from this exercise are then used to propose a generic concept that could support multiple missions
Uveal melanoma cells use ameboid and mesenchymal mechanisms of cell motility crossing the endothelium
Uveal melanomas (UMs) are malignant cancers arising from the pigmented layers of the eye. UM cells spread through the bloodstream, and circulating UM cells are detectable in patients before metastases appear. Extravasation of UM cells is necessary for formation of metastases, and transendothelial migration (TEM) is a key step in extravasation. UM cells execute TEM via a stepwise process involving the actin-based processes of ameboid blebbing and mesenchymal lamellipodial protrusion. UM cancers are driven by oncogenic mutations that activate Gαq/11, and this activates TRIO, a guanine nucleotide exchange factor for RhoA and Rac1. We found that pharmacologic inhibition of Gαq/11 in UM cells reduced TEM. Inhibition of the RhoA pathway blocked amoeboid motility but led to enhanced TEM; in contrast, inhibition of the Rac1 pathway decreased mesenchymal motility and reduced TEM. Inhibition of Arp2/3 complex allowed cells to transmigrate without intercalation, a direct mechanism similar to the one often displayed by immune cells. BAP1-deficient (+/-) UM subclones displayed motility behavior and increased levels of TEM, similar to the effects of RhoA inhibitors. We conclude that RhoA and Rac1 signaling pathways, downstream of oncogenic Gαq/11, combine with pathways regulated by BAP1 to control the motility and transmigration of UM cells
The Black Hole Mass of NGC 4151. II. Stellar Dynamical Measurement from Near-Infrared Integral Field Spectroscopy
We present a revised measurement of the mass of the central black hole (Mbh)
in the Seyfert 1 galaxy NGC 4151. The new stellar dynamical mass measurement is
derived by applying an axisymmetric orbit-superposition code to near-infrared
integral field data obtained using adaptive optics with the Gemini NIFS
spectrograph. When our models attempt to fit both the NIFS kinematics and
additional low spatial resolution kinematics, our results depend sensitively on
how chi-squared is computed--probably a consequence of complex bar kinematics
that manifest immediately outside the nuclear region. The most robust results
are obtained when only the high spatial resolution kinematic constraints in the
nuclear region are included in the fit. Our best estimates for the BH mass and
H-band mass-to-light ratio are Mbh~(3.76+/-1.15)E7 Msun (1-sigma error) and
M/L(H-band)~0.34+/-0.03 Msun/Lsun (3-sigma error), respectively (the quoted
errors reflect the model uncertainties). Our BH mass measurement is consistent
with estimates from both reverberation mapping (3.57[+0.45/-0.37]E7 Msun) and
gas kinematics (3.0[+0.75/-2.2]E7 Msun; 1-sigma errors), and our best-fit
mass-to-light ratio is consistent with the photometric estimate of
M/L(H-band)=0.4+/-0.2 Msun/Lsun. The NIFS kinematics give a central bulge
velocity dispersion sigma_c=116+/-3 km/s, bringing this object slightly closer
to the M-sigma relation for quiescent galaxies. Although NGC 4151 is one of
only a few Seyfert 1 galaxies in which it is possible to obtain a direct
dynamical BH mass measurement--and thus, an independent calibration of the
reverberation mapping mass scale--the complex bar kinematics makes it less than
ideally suited for this purpose.Comment: 21 pages, 15 figures. Accepted for publication in Ap
BAP1 deficiency causes loss of melanocytic cell identity in uveal melanoma
BACKGROUND: Uveal melanoma is a highly aggressive cancer with a strong propensity for metastasis, yet little is known about the biological mechanisms underlying this metastatic potential. We recently showed that most metastasizing uveal melanomas, which exhibit a class 2 gene expression profile, contain inactivating mutations in the tumor suppressor BAP1. The aim of this study was to investigate the role of BAP1 in uveal melanoma progression. METHODS: Uveal melanoma cells were studied following RNAi-mediated depletion of BAP1 using proliferation, BrdU incorporation, flow cytometry, migration, invasion, differentiation and clonogenic assays, as well as in vivo tumorigenicity experiments in NOD-SCID-Gamma mice. RESULTS: Depletion of BAP1 in uveal melanoma cells resulted in a loss of differentiation and gain of stem-like properties, including expression of stem cell markers, increased capacity for self-replication, and enhanced ability to grow in stem cell conditions. BAP1 depletion did not result in increased proliferation, migration, invasion or tumorigenicity. CONCLUSIONS: BAP1 appears to function in the uveal melanocyte lineage primarily as a regulator of differentiation, with cells deficient for BAP1 exhibiting stem-like qualities. It will be important to elucidate how this effect of BAP1 loss promotes metastasis and how to reverse this effect therapeutically
The Mass of the Central Black Hole in the Seyfert Galaxy NGC 4151
In order to improve the reverberation-mapping based estimate of the mass of
the central supermassive black hole in the Seyfert 1 galaxy NGC 4151, we have
reanalyzed archival ultraviolet monitoring spectra from two campaigns
undertaken with the International Ultraviolet Explorer. We measure
emission-line time delays for four lines, C IV 1549, He II 1640, C III] 1909,
and Mg II 2798, from both campaigns. We combine these measurements with the
dispersion of the variable part of each respective emission line to obtain the
mass of the central object. Despite the problematic nature of some of the data,
we are able to measure a mass of 41.1 (+/- 7.3) million solar masses, although
this, like all reverberation-based masses, is probably systematically uncertain
by a factor of 3-4.Comment: 25 pages, 5 figures. Accepted for publication in The Astrophysical
Journa
The Lick AGN Monitoring Project: Reverberation Mapping of Optical Hydrogen and Helium Recombination Lines
We have recently completed a 64-night spectroscopic monitoring campaign at
the Lick Observatory 3-m Shane telescope with the aim of measuring the masses
of the black holes in 12 nearby (z < 0.05) Seyfert 1 galaxies with expected
masses in the range ~10^6-10^7M_sun and also the well-studied nearby active
galactic nucleus (AGN) NGC 5548. Nine of the objects in the sample (including
NGC 5548) showed optical variability of sufficient strength during the
monitoring campaign to allow for a time lag to be measured between the
continuum fluctuations and the response to these fluctuations in the broad
Hbeta emission, which we have previously reported. We present here the light
curves for the Halpha, Hgamma, HeII 4686, and HeI 5876 emission lines and the
time lags for the emission-line responses relative to changes in the continuum
flux. Combining each emission-line time lag with the measured width of the line
in the variable part of the spectrum, we determine a virial mass of the central
supermassive black hole from several independent emission lines. We find that
the masses are generally consistent within the uncertainties. The time-lag
response as a function of velocity across the Balmer line profiles is examined
for six of the AGNs. Finally we compare several trends seen in the dataset
against the predictions from photoionization calculations as presented by
Korista & Goad. We confirm several of their predictions, including an increase
in responsivity and a decrease in the mean time lag as the excitation and
ionization level for the species increases. Further confirmation of
photoionization predictions for broad-line gas behavior will require additional
monitoring programs for these AGNs while they are in different luminosity
states. [abridged]Comment: 37 pages, 18 figures and 15 tables, accepted for publication in the
Astrophysical Journa
Measuring pH variability using an experimental sensor on an underwater glider
Autonomous underwater gliders offer the capability of measuring oceanic parameters continuously at high resolution in both vertical and horizontal planes, with timescales that can extend to many months. An experimental ion sensitive field effect transistor (ISFET) sensor measuring pH on the total scale was attached to a glider during the REP14 – MED experiment in June 2014 in the northwestern Mediterranean Sea. During the deployment, pH was sampled at depths of up to 1000 m, along an 80 km transect over a period of 12 days. Water samples were collected from a nearby ship and analysed for dissolved inorganic carbon concentration and total alkalinity to derive pH for validating the ISFET measurements. The vertical resolution of the pH sensor was good (1 to 2 m), but stability was poor, and the sensor drifted in a non-monotonous fashion. In order to remove the sensor drift, a time-dependent, depth-invariant offset was applied throughout the water column for each dive, reducing the spread of the data by approximately two thirds. Furthermore, the ISFET sensor required temperature and pressure-based corrections, which were achieved using linear regression. Correcting for this decreased the apparent sensor pH variability by a further 13 to 31 %. Sunlight caused an apparent sensor pH decrease of up to 0.1 in surface waters around local noon, highlighting the importance of shielding the sensor away from light in future deployments. The corrected pH from the ISFET sensor is presented along with potential temperature, salinity, potential density anomalies (σθ), and dissolved oxygen concentrations (c(O2)) measured by the glider, providing insights into physical and biogeochemical variability in this region. pH maxima were identified at the depth of the summer chlorophyll maximum, where high c(O2) values were also found. Longitudinal pH variations at depth (σθ > 28.8 kg m−3) highlighted variability of water masses in this region. Higher pH was observed where salinity was > 38.65, and lower pH was found where salinity ranged between 38.3 and 38.65. It seemed that the higher pH was associated with saltier Levantine Intermediate Water. Furthermore, shoaling isopycnals closer to shore coinciding with low pH, high salinity, low c(O2) waters may be indicative of upwelling
Observational Limits on Type 1 AGN Accretion Rate in COSMOS
We present black hole masses and accretion rates for 182 Type 1 AGN in
COSMOS. We estimate masses using the scaling relations for the broad Hb, MgII,
and CIV emission lines in the redshift ranges 0.16<z<0.88, 1<z<2.4, and
2.7<z<4.9. We estimate the accretion rate using an Eddington ratio L_I/L_Edd
estimated from optical and X-ray data. We find that very few Type 1 AGN accrete
below L_I/L_Edd ~ 0.01, despite simulations of synthetic spectra which show
that the survey is sensitive to such Type 1 AGN. At lower accretion rates the
BLR may become obscured, diluted or nonexistent. We find evidence that Type 1
AGN at higher accretion rates have higher optical luminosities, as more of
their emission comes from the cool (optical) accretion disk with respect to
shorter wavelengths. We measure a larger range in accretion rate than previous
works, suggesting that COSMOS is more efficient at finding low accretion rate
Type 1 AGN. However the measured range in accretion rate is still comparable to
the intrinsic scatter from the scaling relations, suggesting that Type 1 AGN
accrete at a narrow range of Eddington ratio, with L_I/L_Edd ~ 0.1.Comment: Accepted for pulication in ApJ. 7 pages, 5 figures, table 1 available
on reques
SkyMapper Southern Survey: First Data Release (DR1)
We present the first data release (DR1) of the SkyMapper Southern Survey, a
hemispheric survey carried out with the SkyMapper Telescope at Siding Spring
Observatory in Australia. Here, we present the survey strategy, data
processing, catalogue construction and database schema. The DR1 dataset
includes over 66,000 images from the Shallow Survey component, covering an area
of 17,200 deg in all six SkyMapper passbands , while the full area
covered by any passband exceeds 20,000 deg. The catalogues contain over 285
million unique astrophysical objects, complete to roughly 18 mag in all bands.
We compare our point-source photometry with PanSTARRS1 DR1 and note an
RMS scatter of 2%. The internal reproducibility of SkyMapper photometry is on
the order of 1%. Astrometric precision is better than 0.2 arcsec based on
comparison with Gaia DR1. We describe the end-user database, through which data
are presented to the world community, and provide some illustrative science
queries.Comment: 31 pages, 19 figures, 10 tables, PASA, accepte
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