Sistematización de una experiencia de extensión : Aportes a la integralidad universitaria

Se presenta la sistematización de una experiencia realizada en el marco del proyecto de extensión “Avicultura Familiar. Organización para la Producción” de la Facultad de Ciencias Veterinarias de la Universidad Nacional de La Plata, durante el periodo comprendido entre el año 2018 hasta el 2020, llevada a cabo en las instalaciones de la facultad Ciencias Veterinarias y en las distintas unidades socio-productivas avícolas, periurbanas y rurales que se encuentran situadas a lo largo de la franja costera comprendida desde la localidad de Punta Lara del partido de Ensenada, hasta la localidad de Pipinas perteneciente al partido de Punta Indio, de la Provincia de Buenos Aires, de la misma participaron docentes, alumnos, no docentes y productores/avicultores. La sistematización se hizo en base a 3 entrevistas, 4 testimonios, datos recopilados en encuestas, material presentado en Jornadas de investigación y divulgación, además de material fotográfico y registros personales de la autora. La sistematización se llevará a cabo y se reflexionará críticamente desde una mirada tendiente a la integralidad entre Docencia, Investigación y Extensión, para poder así intercambiar y compartir aprendizajes con otras experiencias similares.Facultad de Ciencias Veterinaria

Spontaneous Skin Tumor in a Companion Dwarf Rabbit

Background: Scarce reports have been published worldwide on primary skin tumours in pet rabbits. Trichoblastoma is a rare benign neoplasm of animals and humans derived from or reduplicating the primitive germ of embryonic follicular development; it was previously classified as a basal cell tumor, meanwhile its malignant counterpart is referred to as malignant trichoblastoma or trichoblastic carcinoma. Neoplasms of domestic animals that once were lumped into the broad histologic diagnosis of basal cell tumors have since been split into distinct entities, dependent on evidence of differentiation, although a cytologic diagnosis of basal cell tumor continues to be used indistinctly to represent the large, heterogeneous group of epidermal, trichofollicular, and adnexal skin tumors with basal cell characteristics. Hereby, it is described the morphological and immunohistochemical findings of a case of spontaneous malignant trichoblastoma on a domestic companion rabbit from South America. Case: A 4-year-old, male black dwarf rabbit (Oryctolagus cuniculus) was brought to the Veterinary College when appeared with a sudden appearance of a grayish nodular subcutaneous mass measuring 3.0 x 2.0 cm and located on left tarsal zone, during march 2014. A case of malignant trichoblastoma with a predominance of trabecular architecture was diagnosed based on morphologic and microscopic results. Fine-needle aspiration, histopathology and immunohistochemistry were performed on the ulcerated mass. The mass was sectioned and stained with hematoxylin and eosin (HE), periodic acid-Schiff (PAS) and Masson trichrome stain. Replicate serial sections from the paraffin-embedded tissue blocks were immunostained for cytokeratin (AE1/AE3), smooth muscle actin, polyclonal nestin, desmin and myoglobin, using commercial kits. Mayer’s hematoxylin solution was used as a counterstain. Negative controls were produced by substituting the primary antibody with 5% bovine serum albumin in phosphate buffered saline. To assess mitotic activity, mitoses count in 10 randomly selected high-power fields was done. Diagnoses was made in accordance with the recommendations for the histological classification of tumors of domestic animals. After surgical excision, the animal remains healthy. Discussion: Epithelial nonviral skin neoplasms are uncommon in rabbits and have been cited in sporadic case reports or few case series including basal cell tumors and squamous cell carcinomas. All these cases emphasized the need for cytologic criteria and nomenclature on rabbit tumors that better reflect potential variation in tissue differentiation. As a consequence, based on limited current knowledge, the practitioner is left to make decisions for diagnostics and therapeutics in these cases based upon current recommendations for other companion animals with adjustments for lagomorph physiology. This tumor, though variable in size (0.2 to 10 cm in diameter) in domestic animals (i.e., older dogs and cats), it most often appears as a solitary, well-circumscribed hairless mass in the skin, and occasionally ulcerated. The pathological data, that included tumor location, gross appearance, tumor size, growth pattern, cellular atypia, mitosis and immunohistochemistry studies, led to the malignant trichoblastoma diagnosis and these features concurred to those described in the literature. Increasing number of sporadic or induced tumors may be expected in pet rabbits in the veterinary clinic, as these animals have a high consideration among the people, and also they live longer (natural life span range: 5-10 years) than wild or farmed rabbits.Facultad de Ciencias VeterinariasComisión de Investigaciones Científicas de la provincia de Buenos AiresConsejo Nacional de Investigaciones Científicas y Técnica

Detection of Chlamydia psittaci in pet and production birds during March 2013 to March 2014

Las bacterias del género Chlamydia, familia Chlamydiaceae, orden Chlamydiales, son de interés médico, veterinario, zoonótico y ornitológico. Estas bacterias están presentes de manera latente en muchas especies de vertebrados y pueden causar enfermedad sistémica clínicamente evidente en mamíferos, aves, reptiles y anfibios. Las clamidias son de importancia para la salud pública debido al hábito de mantener aves (principalmente psitácidos) como mascotas y representan un riesgo ocupacional, especialmente para veterinarios, trabajadores de mataderos y plantas de procesamiento de mamíferos y aves de corral, personal de plantas de incubación y cuidadores de animales. La clamidiosis, producida por Chlamydia psittaci, es una de las enfermedades zoonóticas mas graves que pueden causar miembros de esta familia, debido a la inhalación de cuerpos elementales presentes en heces y secreciones respiratorias de aves enfermas, sean estas sintomáticas o no. En general los casos en humanos se deben a la exposición frente a aves mascotas infectadas, principalmente psitácidos. Los signos clínicos típicos en aves enfermas son conjuntivitis y descarga nasal mucopurulenta, diarrea, poliuria, decaimiento y mala condición corporal. En humanos los síntomas en general son similares a un cuadro de influenza, pero también pueden darse casos de neumonía severa, endocarditis y encefalitis. El objetivo de este trabajo es determinar la presencia de familia Chlamydiaceae y Chlamydia psittaci (Cp) en muestras de aves mascotas y de producción utilizando dos técnicas de PCR en tiempo real.Facultad de Ciencias Veterinaria

Adenoma de glándula tarsal en cobayo (Cavia porcellus)

Los cobayos son menos susceptibles a padecer neoplasias respecto de otros pequeños mamíferos. Si se asume un máximo de longevidad en esta especie de seis años, la aparición más frecuente es a partir de los tres años de vida. El sistema respiratorio es el sitio más afectado, siguiendo piel y subcutis, en donde la incidencia alcanza un 15%. Los tricofoliculomas son las neoplasias cutáneas más comunes, algunas otras reportadas comprenden adenomas sebáceos, fibromas, fibrosarcomas, lipomas, liposarcomas, schawanomas y linfomas. El adenoma sebáceo ha sido escasamente reportado en el cobayo, siendo generalmente localizado en la región del flanco. En el presente trabajo se realiza la descripción de un adenoma sebáceo de glándula tarsal.Facultad de Ciencias Veterinaria

Multicentre observational study on multisystem inflammatory syndrome related to COVID-19 in Argentina

Background: The impact of the pediatric inflammatory multisystem syndrome temporally associated with SARS-CoV-2 (PIMS-TS) in low- and middle-income countries remains poorly understood. Our aim was to understand the characteristics and outcomes of PIMS-TS in Argentina. Methods: This observational, prospective, and retrospective multicenter study enrolled patients younger than 18 years-old manifesting PIMS-TS, Kawasaki disease (KD) or Kawasaki shock syndrome (KSS) between March 2020 and May 2021. Patients were followed-up until hospital discharge or death (one case). The primary outcome was pediatric intensive care unit (PICU) admission. Multiple logistic regression was used to identify variables predicting PICU admission. Results: Eighty-one percent, 82%, and 14% of the 176 enrolled patients fulfilled the suspect case criteria for PIMS-TS, KD, and KSS, respectively. Temporal association with SARS-CoV-2 was confirmed in 85% of the patients and 38% were admitted to the PICU. The more common clinical manifestations were fever, abdominal pain, rash, and conjunctival injection. Lymphopenia was more common among PICU-admitted patients (87% vs. 51%, p < 0.0001), who also showed a lower platelet count and higher plasmatic levels of inflammatory and cardiac markers. Mitral valve insufficiency, left ventricular wall motion alterations, pericardial effusion, and coronary artery alterations were observed in 30%, 30%, 19.8%, and 18.6% of the patients, respectively. Days to initiation of treatment, rash, lymphopenia, and low platelet count were significant independent contributions to PICU admission. Conclusion: Rates of severe outcomes of PIMS-TS in the present study agreed with those observed in high-income countries. Together with other published studies, this work helps clinicians to better understand this novel clinical entity.Fil: Vainstein, Eduardo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Baleani, Silvia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Urrutia, Luis. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Affranchino, Nicolás. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Ackerman, Judith. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Cazalas, Mariana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Goldsman, Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Sardella, Angela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Tolin, Ana Laura. Gobierno de la Provincia de Mendoza. Hospital Pediátrico Humberto Notti; ArgentinaFil: Goldaracena, Pablo. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños "Sor María Ludovica" de La Plata; ArgentinaFil: Fabi, Mariana. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños "Sor María Ludovica" de La Plata; ArgentinaFil: Cosentino, Mariana. Hospital Británico de Buenos Aires; ArgentinaFil: Magliola, Ricardo. Hospital Británico de Buenos Aires; ArgentinaFil: Roggiero, Gustavo. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic; ArgentinaFil: Manso, Paula. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic; ArgentinaFil: Triguy, Jésica. Gobierno de la Provincia de Mendoza. Hospital Pediátrico Humberto Notti; ArgentinaFil: Ballester, Celeste. Gobierno de la Provincia de Mendoza. Hospital Pediátrico Humberto Notti; ArgentinaFil: Cervetto, Vanesa. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Vaccarello, María. Sanatorio de la Trinidad; ArgentinaFil: De Carli, Domingo Norberto. Clínica del Niño de Quilmes; ArgentinaFil: De Carli, Maria Estela. Clínica del Niño de Quilmes; ArgentinaFil: Ciotti, Ana Laura. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Sicurello, María Irene. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Rios Leiva, Cecilia. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; ArgentinaFil: Villalba, Claudia. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Hortas, María. Sanatorio de la Trinidad; ArgentinaFil: Peña, Sonia. Gobierno de la Provincia de Mendoza. Hospital Pediátrico Humberto Notti; ArgentinaFil: González, Gabriela. Gobierno de la Provincia de Mendoza. Hospital Pediátrico Humberto Notti; ArgentinaFil: Zold, Camila Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Murer, Mario Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Grippo, M.. No especifíca;Fil: Vázquez, H.. No especifíca;Fil: Morós, C.. No especifíca;Fil: Di Santo, M.. No especifíca;Fil: Villa, A.. No especifíca;Fil: Lazota, P.. No especifíca;Fil: Foti, M.. No especifíca;Fil: Napoli, N.. No especifíca;Fil: Katsikas, M. M.. No especifíca;Fil: Tonello, L.. No especifíca;Fil: Peña, J.. No especifíca;Fil: Etcheverry, M.. No especifíca;Fil: Iglesias, D.. No especifíca;Fil: Alcalde, A. L.. No especifíca;Fil: Bruera, M.J.. No especifíca;Fil: Bruzzo, V.. No especifíca;Fil: Giordano, P.. No especifíca;Fil: Pena Acero, F.. No especifíca;Fil: Netri Pelandi, G.. No especifíca;Fil: Pastaro, D.. No especifíca;Fil: Bleiz, J.. No especifíca;Fil: Rodríguez, M. F.. No especifíca;Fil: Laghezza, L.. No especifíca;Fil: Molina, M. B.. No especifíca;Fil: Patynok, N.. No especifíca;Fil: Chatelain, M. S.. No especifíca;Fil: Aguilar, M. J.. No especifíca;Fil: Gamboa, J.. No especifíca;Fil: Cervan, M.. No especifíca;Fil: Ruggeri, A.. No especifíca;Fil: Marinelli, I.. No especifíca;Fil: Checcacci, E.. No especifíca;Fil: Meregalli, C.. No especifíca;Fil: Damksy Barbosa, J.. No especifíca;Fil: Fernie, L.. No especifíca;Fil: Fernández, M. J.. No especifíca;Fil: Saenz Tejeira, M.M.. No especifíca;Fil: Cereigido, C.. No especifíca;Fil: Nunell, A.. No especifíca;Fil: Villar, D.. No especifíca;Fil: Mansilla, A. D.. No especifíca;Fil: Darduin, M. D.. No especifíca

Spontaneous Skin Tumor in a Companion Dwarf Rabbit

Background: Scarce reports have been published worldwide on primary skin tumours in pet rabbits. Trichoblastoma is a rare benign neoplasm of animals and humans derived from or reduplicating the primitive germ of embryonic follicular development; it was previously classified as a basal cell tumor, meanwhile its malignant counterpart is referred to as malignant trichoblastoma or trichoblastic carcinoma. Neoplasms of domestic animals that once were lumped into the broad histologic diagnosis of basal cell tumors have since been split into distinct entities, dependent on evidence of differentiation, although a cytologic diagnosis of basal cell tumor continues to be used indistinctly to represent the large, heterogeneous group of epidermal, trichofollicular, and adnexal skin tumors with basal cell characteristics. Hereby, it is described the morphological and immunohistochemical findings of a case of spontaneous malignant trichoblastoma on a domestic companion rabbit from South America.Case: A 4-year-old, male black dwarf rabbit (Oryctolagus cuniculus) was brought to the Veterinary College when appeared with a sudden appearance of a grayish nodular subcutaneous mass measuring 3.0 x 2.0 cm and located on left tarsal zone, during march 2014. A case of malignant trichoblastoma with a predominance of trabecular architecture was diagnosed based on morphologic and microscopic results. Fine-needle aspiration, histopathology and immunohistochemistry were performed on the ulcerated mass. The mass was sectioned and stained with hematoxylin and eosin (HE), periodic acid-Schiff (PAS) and Masson trichrome stain. Replicate serial sections from the paraffin-embedded tissue blocks were immunostained for cytokeratin (AE1/AE3), smooth muscle actin, polyclonal nestin, desmin and myoglobin, using commercial kits. Mayer’s hematoxylin solution was used as a counterstain. Negative controls were produced by substituting the primary antibody with 5% bovine serum albumin in phosphate buffered saline. To assess mitotic activity, mitoses count in 10 randomly selected high-power fields was done. Diagnoses was made in accordance with the recommendations for the histological classification of tumors of domestic animals. After surgical excision, the animal remains healthy.Discussion: Epithelial nonviral skin neoplasms are uncommon in rabbits and have been cited in sporadic case reports or few case series including basal cell tumors and squamous cell carcinomas. All these cases emphasized the need for cytologic criteria and nomenclature on rabbit tumors that better reflect potential variation in tissue differentiation. As a consequence, based on limited current knowledge, the practitioner is left to make decisions for diagnostics and therapeutics in these cases based upon current recommendations for other companion animals with adjustments for lagomorph physiology. This tumor, though variable in size (0.2 to 10 cm in diameter) in domestic animals (i.e., older dogs and cats), it most often appears as a solitary, well-circumscribed hairless mass in the skin, and occasionally ulcerated. The pathological data, that included tumor location, gross appearance, tumor size, growth pattern, cellular atypia, mitosis and immunohistochemistry studies, led to the malignant trichoblastoma diagnosis and these features concurred to those described in the literature. Increasing number of sporadic or induced tumors may be expected in pet rabbits in the veterinary clinic, as these animals have a high consideration among the people, and also they live longer (natural life span range: 5-10 years) than wild or farmed rabbits

Primer reporte de Coccidiosis renal fatal en un Biguá (Phalacrocorax brasilianus) en coinfección con nematodes de Argentina

After being rescued from a state of weakness, a biguá (Phalacrocorax brasilianus) specimen under clinical management conditions underwent a remarkable general improvement before abruptly deteriorating until dying within a few hours as a result of acute renal failure. Clinical analysis and post-mortem histopathological findings demonstrated the presence of a large burden of gastrointestinal nematodes and severe renal involvement by Eimeriorina representatives. These latter were found to be the cause of extensive kidney damage sufficient to cause acute renal failure and to be considered the primary cause of death. Throughout this work, we obtained an overview of the pathogenesis and clinical manifestations of avian renal coccidiosis, which had been poorly documented previously. This coccidial finding, never before reported in this species, implies a high probability of recording a new species of Eimeria.Un espécimen de biguá (Phalacrocorax brasilianus) estando bajo condiciones de manejo clínico, luego de ser rescatado en estado de debilidad, experimentó una marcada mejoría general para luego sufrir un cambio abrupto hacia una evolución desfavorable hasta desenlace fatal en el curso de pocas horas debido a una falla renal aguda. El análisis de resultados de los estudios clínicos y de los hallazgos histopatológicos post mortem demostraron la presencia de una gran carga de nematodes gastrointestinales y de severa afección renal por Eimeriorinos. Estos últimos resultaron ser los causales de un extensivo daño renal suficiente para causar la falla renal aguda y considerarse la causa primaria de muerte. A lo largo de este trabajo se obtuvo una visión general de la patogenia y manifestaciones clínicas de la coccidiosis renal aviar, escasamente documentadas anteriormente. Este hallazgo coccidial nunca antes reportado en esta especie implica una alta probabilidad de registro de una nueva especie de Eimeria