Antioxidant and antiproliferative activities of the essential oils from Thymbra capitata and Thymus Species grown in Portugal

The antioxidant and antiproliferative activities of the essential oils from Thymbra capitata and Thymus species grown in Portugal were evaluated. Thymbra and Thymus essential oils were grouped into two clusters: Cluster I in which carvacrol, thymol, p-cymene, alpha-terpineol, and gamma-terpinene dominated and Cluster II in which thymol and carvacrol were absent and the main constituent was linalool. The ability for scavenging ABTS(center dot+) and peroxyl free radicals as well as for preventing the growth of THP-1 leukemia cells was better in essential oils with the highest contents of thymol and carvacrol. These results show the importance of these two terpene-phenolic compounds as antioxidants and cytotoxic agents against THP-1 cells.Fundacao para a Ciencia e Tecnologia (FCT) [Pest-OE/EQB/LA0023/2011

Análisis de la percepción del alumnado ante la metodología de trabajar en grupos tutorizados

[EN] The main objective of this paper is to analyze the opinion and perspective that the students have in view of the methodology of working in tutored groups. This methodology adopted in the teaching-learning process is important for the development of competences. The ability to work in a team and the ability to communicate are very significant transversal competences that have a great impact or potential in student learning. This paper presents an analysis of student assessments collected through anonymous opinions after the elaboration of a tutorial work carried out in a group in the Medicine Degree of the University of Valencia during the years 2015-2016 and 2016- 2017. It is essential to take into account the opinion of the students to adopt measures of teacher improvement, correct or modify the methodology and solve situations arising in the real learning environment. Our conclusion is the students' perspective is very positive and they like the methodology of working in tutored groups.[ES] El objetivo de este trabajo es analizar la opinión y perspectiva que tiene el alumnado ante la metodología de trabajar en grupos tutorizados. Esta metodología adoptada en el proceso de enseñanza-aprendizaje es importante para el desarrollo de competencias. La capacidad de trabajo en equipo y la capacidad para comunicarse son competencias transversales muy significativas y que tienen un gran impacto o potencial en el aprendizaje del alumno. Se presenta en este trabajo un análisis de las valoraciones del alumnado recogidas mediante opiniones anónimas después de la elaboración de un trabajo tutorizado realizado en grupo en el Grado de Medicina de la Universitat de València durante los cursos 2015-2016 y 2016-2017. Es fundamental tener en cuenta la opinión del alumnado para adoptar medidas de mejora docente, corregir o modificar la metodología y resolver situaciones surgidas en el entorno real de aprendizaje. Nuestra conclusión es que la perspectiva del alumnado es muy positiva que les gusta la metodología de trabajar en grupos tutorizados.Serna, E.; San Miguel, T.; Megias, J.; Mauricio, MD. (2017). Análisis de la percepción del alumnado ante la metodología de trabajar en grupos tutorizados. En In-Red 2017. III Congreso Nacional de innovación educativa y de docencia en red. Editorial Universitat Politècnica de València. 999-1008. https://doi.org/10.4995/INRED2017.2017.6843OCS999100

Cost-Effectiveness of Therapeutic Drug Monitoring of Anti-TNF Therapy in Inflammatory Bowel Disease: A Systematic Review

Infliximab and adalimumab are monoclonal antibodies against tumor necrosis factor (anti-TNF) used to manage inflammatory bowel disease (IBD). Therapeutic Drug Monitoring (TDM) has been proven to prevent immunogenicity, to achieve better long-term clinical results and to save costs in IBD treatment. The aim of this study was to conduct a systematic review on cost-effectiveness analyses of studies that apply TDM of anti-TNF in IBD and to provide a critical analysis of the best scientific knowledge available in the literature. The quality of the included studies was assessed using Consolidated Health Economic Evaluation Reporting Standards (CHEERS). Cost-effectiveness of the TDM strategies was presented as total costs, cost savings, quality-adjusted life-years (QALY) and incremental cost-effectiveness ratio (ICER). Thirteen studies that examined the health economics of TDM of anti-TNF in IBD from 2013 to 2021 were included. Eight of them (61.5%) achieved a score between 17 and 23 on the CHEERS checklist. The comparison between the TDM strategy and an empirical strategy was cost saving. The ICER between reactive TDM and an empirical strategy was dominated (favorable) by reactive TDM, whereas the ICER value for proactive TDM compared to an empirical strategy ranged from EUR 56,845 to 3,901,554. This systematic review demonstrated that a TDM strategy is cost-effective or cost-saving in IBD.S.M.-M. received a predoctoral fellowship from Miguel Hernandez University (“Ayudas a la contratación de personal investigador en formación 2021”).S

Bio-Guided Isolation of Acetogenins from Annona cherimola Deciduous Leaves: Production of Nanocarriers to Boost the Bioavailability Properties

Annonaceous acetogenins (ACGs) are lipophilic polyketides isolated exclusively from Annonaceae. They are considered to be amongst the most potent antitumor compounds. Nevertheless, their applications are limited by their poor solubility. The isolation of ACGs from Annona cherimola leaves, an agricultural waste, has not been reported to date. Molvizarin (1) cherimolin-1 (2), motrilin (3), annonacin (4) and annonisin (5) are isolated for the first time from A. cherimola deciduous leaves. Annonacin was found to be four- and two-times more potent in tumoral cells (HeLa, 23.6% live cells; IGROV-1, 40.8% live cells for 24 h) than in HEK-293 at 50 mu M (24 h, 87.2% live cells). Supramolecular polymer micelles (SMPMs) were synthesized to encapsulate the major ACG isolated, annonacin, in order to improve its solubility in aqueous media. The bioavailability of this compound was increased by a factor of 13 in a simulated human digestive system when compared with free annonacin and an encapsulation efficiency of 35% was achieved. In addition, the cytotoxic activity of SMPMs that hosted annonacin (100 mu M, 24 h, 5.8% live cells) was increased compared with free annonacin in water (100 mu M, 24 h, 92% live cells). These results highlight the use of by-products of A. cherimola, and their pure compounds, as a promising source of anticancer agents. The use of SMPMs as nanocarriers of ACGs could be an alternative for their application in food field as nutraceutical to enhance the administration and efficacy

La motivación de las tareas digitales mediante “pseudo-ApS” en Biología Celular del grado de Medicina

[ES] Los grupos tutorizados de la asignatura “Biología” de 1º de Medicina (Universitat de València) son un entorno estupendo en el que desarrollas actividades que aúnen el carácter formativo conceptual con el desarrollo competencial transversal. Para ello, en el curso 19-20, hemos implementado la vinculación de la cuenta institucional UV con Microsoft 365, gracias al convenio existente entre ambas entidades para facilitar la utilización de las Tecnologías de la Información y la Comunicación (TICs) en la gestión del tiempo y del intercambio de información. Se propone a los alumnos la elaboración de objetos de aprendizaje propios y originales, en formato animación, que serán utilizados durante los próximos dos años por otros estudiantes y en último término, por asociaciones externas, en un proyecto que trata de aunar el Aprendizaje-Servicio con la utilización de las TIC en un contexto en que el alumno se hace responsable de su aprendizaje y del de sus compañeros.[EN] The tutored groups of the subject “Biology” of 1st year of Medicine (Universitat de València) are a wonderful environment in which you develop activities that combine the character of conceptual training with the transversal development of competences. For this purpose, during the academic year 19-20, we have implemented the link between the UV institutional account with Microsoft 365, thanks to the existing agreement between both entities to facilitate the use of Information and Communication Technologies (ICTs) in the management of the time and information exchange. Students are proposed to create their own original learning objects, in animation format, which will be used over the next two years by other students and ultimately by external associations, in a project that tries to combine Service-Learning with the use of ICT in a context in which students are responsible for their own learning and that of his classmates.San-Miguel, T.; Megias, J.; Serna, E.; Calabuig, S.; Morales, JM.; Montoliu, C.; Monleón, D. (2021). La motivación de las tareas digitales mediante “pseudo-ApS” en Biología Celular del grado de Medicina. En IN-RED 2020: VI Congreso de Innovación Educativa y Docencia en Red. Editorial Universitat Politècnica de València. 866-873. https://doi.org/10.4995/INRED2020.2020.11966OCS86687

Arquitetura de hospital e cuidados durante a XV ao século XIX

El presente texto pretende ofrecer un acercamiento a la arquitectura para la curación y el cuidado, revisando los diferentes modelos arquitectónicos, que desde el siglo XV se han venido desarrollando a lo largo de la Historia de la Humanidad para poder dar una respuesta a las necesidades de salud que se han originado hasta llegar a los nuevos ejemplos que surgen tras los descubrimientos científicos propios del siglo XIX, produciéndose un giro importante en la concepción del edificio hospitalario. El breve recorrido por la arquitectura hospitalaria, que se centra en el panorama europeo, además aborda la importancia que presentó en su momento, y que nos ha llegado hasta la actualidad, la figura de Florence Nightingale, tanto en el entorno anglosajón, como en el resto del planeta.This text aims to offer an approach to architecture for healing and care, reviewing the different architectural models, since the fifteenth century have been developed throughout the history of humanity to respond to the needs of health have sprung up to the new examples that arise after their own scientific discoveries of the nineteenth century, a shift in the conception of the hospital building. The brief tour of the hospital architecture, which focuses on the European scene also addresses the importance presented at the time, and has survived until today, the figure of Florence Nightingale, in the Anglo-Saxon environment, such as the rest of the planet.Este texto destina-se a fornecer uma abordagem de arquitetura para a cura e cuidado, analisando diferentes modelos arquitectónicos, desde o século XV foram desenvolvido ao longo da história da humanidade para dar uma resposta às necessidades de saúde originadas até chegar os novos exemplos que surgem após as descobertas científicas do século XIX produzindo uma mudança significativa na concepção do edifício hospitalar. A breve turnê da arquitetura do hospital, que concentra-se na cena europeia, também aborda a importância que apresentou no momento, e que nos chegou até agora, a figura de Florence Nightingale, o ambiente de anglo-saxão, ambos no resto do planeta

El método del caso en la enseñanza del Derecho Mercantil (FINAL): Derecho de sociedades

El proyecto de innovación docente “El método del caso en la enseñanza del Derecho Mercantil (FINAL): Derecho de sociedades” supone el cierre del proyecto que se inicia en la convocatoria de Proyectos de Innovación Docente del curso 2017/2018 y se continúa en la edición de los Proyectos de Innovación Docente de 2018/2019 bajo el mismo título (I y II), cuyos investigadores principales fueron los profesores Megías López y Guerrero Trevijano, respectivamente. La finalidad de conjunto de estos tres proyectos ha sido la de analizar las posibilidades de implementación del conocido como “método del caso” en la Facultad de Derecho de la Universidad Complutense; método que se fundamenta, esencialmente, en una aproximación a la enseñanza desde la perspectiva de la dialéctica socrática, en la que el profesor propone preguntas a los alumnos que, tras un estudio previo de las cuestiones teóricas y los supuestos prácticos que se discutirán en la sesión, profundizan en el conocimiento del contenido de la asignatura mediante el debate en el aula, aplicando al caso concreto los conocimientos teóricos previamente adquiridos.Depto. de Derecho Mercantil, Financiero y TributarioFac. de DerechoFALSEsubmitte

Effectiveness of Gold Nanorods of Different Sizes in Photothermal Therapy to Eliminate Melanoma and Glioblastoma Cells

Gold nanorods are the most commonly used nanoparticles in photothermal therapy for cancer treatment due to their high efficiency in converting light into heat. This study aimed to investigate the efficacy of gold nanorods of different sizes (large and small) in eliminating two types of cancer cell: melanoma and glioblastoma cells. After establishing the optimal concentration of nanoparticles and determining the appropriate time and power of laser irradiation, photothermal therapy was applied to melanoma and glioblastoma cells, resulting in the highly efficient elimination of both cell types. The efficiency of the PTT was evaluated using several methods, including biochemical analysis, fluorescence microscopy, and flow cytometry. The dehydrogenase activity, as well as calcein-propidium iodide and Annexin V staining, were employed to determine the cell viability and the type of cell death triggered by the PTT. The melanoma cells exhibited greater resistance to photothermal therapy, but this resistance was overcome by irradiating cells at physiological temperatures. Our findings revealed that the predominant cell-death pathway activated by the photothermal therapy mediated by gold nanorods was apoptosis. This is advantageous as the presence of apoptotic cells can stimulate antitumoral immunity in vivo. Considering the high efficacy of these gold nanorods in photothermal therapy, large nanoparticles could be useful for biofunctionalization purposes. Large nanorods offer a greater surface area for attaching biomolecules, thereby promoting high sensitivity and specificity in recognizing target cancer cells. Additionally, large nanoparticles could also be beneficial for theranostic applications, involving both therapy and diagnosis, due to their superior detection sensitivity.Ministerio de Ciencia, Innovación y Universidades (España)Depto. de Química FísicaFac. de Ciencias QuímicasTRUEpubDescuento UC