Evaluation of the mechanical and physical properties of a posterior resin composite in posterior adult teeth

To evaluate the mechanical and physical properties of a micro-hybrid resin composite used in adult posterior restorations A micro-hybrid, light curing resin composite Unolux BCS Composite Restorative, (UnoDent, England) was used to restore 74 carious classes I and II cavities on posterior teeth of 62 adult patients. The restorations were evaluated immediately following placement (Baseline), at 1 week, 3months, 6months and12months using the United States Public Health Service Criteria/Modified Ryge criteria for direct evaluation. Color matching, marginal stains and adaptation, wear and surface texture were evaluated. Ranging from best tothe worst, the ratings were,Alfa, Bravo and Charlie. 58 restorations were available for review at the 12 month evaluation, 15 patients bearing 21.6% of the restorations were lost to recall. Colour match scores were 89.6% Alpha at baseline and 74.1% at 12-month review. Marginal staining were 100%Alpha at baseline and 98.3%Alpha at the end of the evaluation period. Marginal adaptation at baseline was 100%Alpha this value dropped to 94.8% by the 12 month.Anatomical wear scores were 100%Alpha at baseline and scores dropped to 93.1% at the 12-month evaluation.At baseline,Alpha scores for Surface texture were 100%.Adrop inAlpha scores to 93.1% at the first week review was maintained till the 12 month.Carefully controlled placement of micro-hybrid resin composite using the total etch and type 2 (one-bottle ) adhesive can produce satisfactory posterior restorations on permanent teeth

Evaluation of post-operative sensitivity and secondary caries in posterior composite restorations: A 12month study

To evaluate post-operative sensitivity and secondary caries associated with posterior composite restoration. The study involved restoration of occlusal and proximo-occlusal caries on premolars and molars of 62 patients seen at the out-patient clinic of the restorative department of the Lagos University Teaching Hospital. The restorations were completed with a micro-hybrid light curing resin composite (Unolux BCS, UnoDent, England). Following total etch procedure and use of a type 2 (5 generation/one-bottle) adhesive. The USPHSC or modified Ryge criteria was employed for direct evaluation of the restorations over the 12 month period.Post-operative sensitivity was 3.5% Bravo at baseline and 1.7% Bravo at 12 months. No significant change in result was recorded for post-operative sensitivity at the end of the evaluation period. 100% was recorded for the absence of secondary caries throughout the evaluation period up till the 6 month when a failed restoration scored 1.7% Bravo.At 12 monthsAlpha scores for secondary caries was 98.3%. Post-operative sensitivity was kept to a minimum and there were no occurrences of secondary caries

Effective and Efficient Masking with Low Noise Using Small-Mersenne-Prime Ciphers

Embedded devices used in security applications are natural targets for physical attacks. Thus, enhancing their side-channel resistance is an important research challenge. A standard solution for this purpose is the use of Boolean masking schemes, as they are well adapted to current block ciphers with efficient bitslice representations. Boolean masking guarantees that the security of an implementation grows exponentially in the number of shares under the assumption that leakages are sufficiently noisy (and independent). Unfortunately, it has been shown that this noise assumption is hardly met on low-end devices. In this paper, we therefore investigate techniques to mask cryptographic algorithms in such a way that their resistance can survive an almost complete lack of noise. Building on seed theoretical results of Dziembowski et al., we put forward that arithmetic encodings in prime fields can reach this goal. We first exhibit the gains that such encodings lead to thanks to a simulated information theoretic analysis of their leakage (with up to six shares). We then provide figures showing that on platforms where optimized arithmetic adders and multipliers are readily available (i.e., most MCUs and FPGAs), performing masked operations in small to medium Mersenne-prime fields as opposed to binary extension fields will not lead to notable implementation overheads. We compile these observations into a new AES-like block cipher, called AES-prime, which is well-suited to illustrate the remarkable advantages of masking in prime fields. We also confirm the practical relevance of our findings by evaluating concrete software (ARM Cortex-M3) and hardware (Xilinx Spartan-6) implementations. Our experimental results show that security gains over Boolean masking (and, more generally, binary encodings) can reach orders of magnitude despite the same amount of information being leaked per share

Different rates of defense evolution and niche preferences in clonal and nonclonal milkweeds (Asclepias spp.)

Summary Given the dual role of many plant traits to tolerate both herbivore attack and abiotic stress, the climatic niche of a species should be integrated into the study of plant defense strategies. Here we investigate the impact of plant reproductive strategy and components of species' climatic niche on the rate of chemical defense evolution in the milkweeds using a common garden experiment of 49 species. We found that across Asclepias species, clonal reproduction repeatedly evolved in lower temperature conditions, in species generally producing low concentrations of a toxic defense (cardenolides). Additionally, we found that rates of cardenolide evolution were lower for clonal than for nonclonal species. We thus conclude that because the clonal strategy is based on survival, long generation times, and is associated with tolerance of herbivory, it may be an alternative to toxicity in colder ecosystems. Taken together, these results indicate that the rate of chemical defense evolution is influenced by the intersection of life-history strategy and climatic niches into which plants radiate

Male Microchimerism at High Levels in Peripheral Blood Mononuclear Cells from Women with End Stage Renal Disease before Kidney Transplantation

Patients with end stage renal diseases (ESRD) are generally tested for donor chimerism after kidney transplantation for tolerance mechanism purposes. But, to our knowledge, no data are available on natural and/or iatrogenic microchimerism (Mc), deriving from pregnancy and/or blood transfusion, acquired prior to transplantation. In this context, we tested the prevalence of male Mc using a real time PCR assay for DYS14, a Y-chromosome specific sequence, in peripheral blood mononuclear cells (PBMC) from 55 women with ESRD, prior to their first kidney transplantation, and compared them with results from 82 healthy women. Male Mc was also quantified in 5 native kidney biopsies obtained two to four years prior to blood testing and in PBMC from 8 women collected after female kidney transplantation, several years after the initial blood testing. Women with ESRD showed statistically higher frequencies (62%) and quantities (98 genome equivalent cells per million of host cells, gEq/M) of male Mc in their PBMC than healthy women (16% and 0.3 gEq/M, p<0.00001 and p = 0.0005 respectively). Male Mc was increased in women with ESRD whether they had or not a history of male pregnancy and/or of blood transfusion. Three out of five renal biopsies obtained a few years prior to the blood test also contained Mc, but no correlation could be established between earlier Mc in a kidney and later presence in PBMC. Finally, several years after female kidney transplantation, male Mc was totally cleared from PBMC in all women tested but one. This intriguing and striking initial result of natural and iatrogenic male Mc persistence in peripheral blood from women with ESRD raises several hypotheses for the possible role of these cells in renal diseases. Further studies are needed to elucidate mechanisms of recruitment and persistence of Mc in women with ESRD

Prioritising surveillance for alien organisms transported as stowaways on ships travelling to South Africa

The global shipping network facilitates the transportation and introduction of marine and terrestrial organisms to regions where they are not native, and some of these organisms become invasive. South Africa was used as a case study to evaluate the potential for shipping to contribute to the introduction and establishment of marine and terrestrial alien species (i.e. establishment debt) and to assess how this varies across shipping routes and seasons. As a proxy for the number of species introduced (i.e. 'colonisation pressure') shipping movement data were used to determine, for each season, the number of ships that visited South African ports from foreign ports and the number of days travelled between ports. Seasonal marine and terrestrial environmental similarity between South African and foreign ports was then used to estimate the likelihood that introduced species would establish. These data were used to determine the seasonal relative contribution of shipping routes to South Africa's marine and terrestrial establishment debt. Additionally, distribution data were used to identify marine and terrestrial species that are known to be invasive elsewhere and which might be introduced to each South African port through shipping routes that have a high relative contribution to establishment debt. Shipping routes from Asian ports, especially Singapore, have a particularly high relative contribution to South Africa's establishment debt, while among South African ports, Durban has the highest risk of being invaded. There was seasonal variation in the shipping routes that have a high relative contribution to the establishment debt of the South African ports. The presented method provides a simple way to prioritise surveillance effort and our results indicate that, for South Africa, port-specific prevention strategies should be developed, a large portion of the available resources should be allocated to Durban, and seasonal variations and their consequences for prevention strategies should be explored further. (Résumé d'auteur

Multisensory information facilitates reaction speed by enlarging activity difference between superior colliculus hemispheres in rats

Animals can make faster behavioral responses to multisensory stimuli than to unisensory stimuli. The superior colliculus (SC), which receives multiple inputs from different sensory modalities, is considered to be involved in the initiation of motor responses. However, the mechanism by which multisensory information facilitates motor responses is not yet understood. Here, we demonstrate that multisensory information modulates competition among SC neurons to elicit faster responses. We conducted multiunit recordings from the SC of rats performing a two-alternative spatial discrimination task using auditory and/or visual stimuli. We found that a large population of SC neurons showed direction-selective activity before the onset of movement in response to the stimuli irrespective of stimulation modality. Trial-by-trial correlation analysis showed that the premovement activity of many SC neurons increased with faster reaction speed for the contraversive movement, whereas the premovement activity of another population of neurons decreased with faster reaction speed for the ipsiversive movement. When visual and auditory stimuli were presented simultaneously, the premovement activity of a population of neurons for the contraversive movement was enhanced, whereas the premovement activity of another population of neurons for the ipsiversive movement was depressed. Unilateral inactivation of SC using muscimol prolonged reaction times of contraversive movements, but it shortened those of ipsiversive movements. These findings suggest that the difference in activity between the SC hemispheres regulates the reaction speed of motor responses, and multisensory information enlarges the activity difference resulting in faster responses

Perspectives on the Trypanosoma cruzi-host cell receptor interaction

Chagas disease is caused by the parasite Trypanosoma cruzi. The critical initial event is the interaction of the trypomastigote form of the parasite with host receptors. This review highlights recent observations concerning these interactions. Some of the key receptors considered are those for thromboxane, bradykinin, and for the nerve growth factor TrKA. Other important receptors such as galectin-3, thrombospondin, and laminin are also discussed. Investigation into the molecular biology and cell biology of host receptors for T. cruzi may provide novel therapeutic targets

Silencing cytokeratin 18 gene inhibits intracellular replication of Trypanosoma cruzi in HeLa cells but not binding and invasion of trypanosomes

<p>Abstract</p> <p>Background</p> <p>As an obligatory intracellular parasite, <it>Trypanosoma cruzi</it>, the etiological agent of Chagas' disease, must invade and multiply within mammalian cells. Cytokeratin 18 (CK18) is among the host molecules that have been suggested as a mediator of important events during <it>T. cruzi</it>-host cell interaction. Based on that possibility, we addressed whether RNA interference (RNAi)-mediated down regulation of the CK18 gene could interfere with the parasite life cycle <it>in vitro</it>. HeLa cells transiently transfected with CK18-RNAi had negligible levels of CK18 transcripts, and significantly reduced levels of CK18 protein expression as determined by immunoblotting or immunofluorescence.</p> <p>Results</p> <p>CK18 negative or positive HeLa cells were invaded equally as well by trypomastigotes of different <it>T. cruzi </it>strains. Also, in CK18 negative or positive cells, parasites recruited host cells lysosomes and escaped from the parasitophorous vacuole equally as well. After that, the growth of amastigotes of the Y or CL-Brener strains, was drastically arrested in CK18 RNAi-treated cells. After 48 hours, the number of amastigotes was several times lower in CK18 RNAi-treated cells when compared to control cells. Simultaneous staining of parasites and CK18 showed that in HeLa cells infected with the Y strain both co-localize. Although the amastigote surface protein-2 contains the domain VTVXNVFLYNR previously described to bind to CK18, in several attempts, we failed to detect binding of a recombinant protein to CK-18.</p> <p>Conclusion</p> <p>The study demonstrates that silencing CK18 by transient RNAi, inhibits intracellular multiplication of the Y and CL strain of <it>T. cruzi </it>in HeLa cells, but not trypanosome binding and invasion.</p